BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0022.p (274 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK80.1 B3KK80_9BACI Purine nucleoside phosphorylase I, inosine... 397 e-109 B1DJC3.1 B1DJC3_9BACL Purine nucleoside phosphorylase I, inosine... 365 2e-99 C2WBK3.1 C2WBK3_BACCE Purine nucleoside phosphorylase 332 3e-89 A9VFJ1.1 A9VFJ1_BACWK Purine nucleoside phosphorylase I, inosine... 331 3e-89 C3AAD0.1 C3AAD0_BACMY Purine nucleoside phosphorylase 331 3e-89 >B3KK80.1 B3KK80_9BACI Purine nucleoside phosphorylase I, inosine and guanosine-specific Length = 274 Score = 397 bits (1020), Expect = e-109, Method: Compositional matrix adjust. Identities = 198/274 (72%), Positives = 221/274 (80%) Query: 1 MNVVTQSNIQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTV 60 M+ V Q +I+EAA YI+ + V PE A+LI + V I Y +IPHFPVSTV Sbjct: 1 MSSVHQQSIKEAAAYIRGQNGVQPEVGLILGSGLGVLAELIENPVSIAYTDIPHFPVSTV 60 Query: 61 EGHEGELLIGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVN 120 EGHEGELL+G I R VV+MKGRFHMYEGYGPE TAFPVRVMKELGV SLLVTNAAGG+N Sbjct: 61 EGHEGELLLGTIHDRPVVLMKGRFHMYEGYGPEVTAFPVRVMKELGVKSLLVTNAAGGIN 120 Query: 121 TDFTPGDLMLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFE 180 T F PGDLMLI+DHLN+TG NPL GPND ALGVRFPDMS AYS +L +AKE A QG Sbjct: 121 TSFEPGDLMLISDHLNMTGTNPLIGPNDPALGVRFPDMSQAYSRQLRQLAKEVAVGQGIV 180 Query: 181 FKEGVYAGLLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAA 240 +EGVYAGLLGP YETPAEIVMLR GADAVGMSTVSE IVARHAG+EVLGISCI+NMAA Sbjct: 181 LREGVYAGLLGPTYETPAEIVMLRTLGADAVGMSTVSEVIVARHAGLEVLGISCISNMAA 240 Query: 241 GILDQPLNHAEVMETADRVREHFLKLVLALIPKM 274 GILDQPL+H EVMETA++VRE FL LV+A+IPKM Sbjct: 241 GILDQPLSHDEVMETAEKVREKFLALVMAIIPKM 274 >B1DJC3.1 B1DJC3_9BACL Purine nucleoside phosphorylase I, inosine and guanosine-specific Length = 276 Score = 365 bits (938), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 178/268 (66%), Positives = 209/268 (77%) Query: 4 VTQSNIQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTVEGH 63 +T + I+EAA +I K V PE D I + V I Y +IPHFPVSTVEGH Sbjct: 6 MTAAQIKEAASFIAGKVQVKPEIGLIMGSGLGVLGDHIENPVTIDYSDIPHFPVSTVEGH 65 Query: 64 EGELLIGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTDF 123 GEL++G + GR VV+M+GRFHMYEGYGPE TAFPVRVMK LGV +LLVTNAAGGVN D+ Sbjct: 66 AGELVVGNLSGRAVVLMRGRFHMYEGYGPELTAFPVRVMKALGVTALLVTNAAGGVNLDY 125 Query: 124 TPGDLMLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFKE 183 G+LMLI+DHLN+TG+NPL GPNDN LGVRFPDMS AYS RL A+A++ AA QG + +E Sbjct: 126 EAGNLMLISDHLNMTGKNPLIGPNDNELGVRFPDMSEAYSKRLRAIARDTAAKQGLKLQE 185 Query: 184 GVYAGLLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGIL 243 GVYAGLLGP YETPAEI MLR GADAVGMSTV+E I ARH+GIEVLGISCI+NMAAG+L Sbjct: 186 GVYAGLLGPTYETPAEIRMLRVLGADAVGMSTVAEVIAARHSGIEVLGISCISNMAAGVL 245 Query: 244 DQPLNHAEVMETADRVREHFLKLVLALI 271 DQPL+H EV+ET +RV+ FL LV +I Sbjct: 246 DQPLSHDEVIETTERVKAEFLALVKEVI 273 >C2WBK3.1 C2WBK3_BACCE Purine nucleoside phosphorylase Length = 273 Score = 332 bits (850), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 159/266 (59%), Positives = 199/266 (74%) Query: 9 IQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTVEGHEGELL 68 I ++A Y+K+K P+ AD I + V +PY EIP FPVSTVEGH G+L+ Sbjct: 6 ITKSASYLKEKFQETPQVGLILGSGLGVLADEIENAVTVPYSEIPEFPVSTVEGHAGQLV 65 Query: 69 IGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTDFTPGDL 128 G ++G VV M+GRFH YEGY + FPVRVMKELGV +++VTNAAGGVNT+F PGDL Sbjct: 66 FGTLQGVTVVAMQGRFHFYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTEFEPGDL 125 Query: 129 MLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFKEGVYAG 188 MLI+DH+N G NPL GPND+ +GVRFPDMST+Y+P L +AK+ AA + +EGVY G Sbjct: 126 MLISDHINFMGTNPLIGPNDSEMGVRFPDMSTSYTPELREMAKQVAADLNIKVQEGVYVG 185 Query: 189 LLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGILDQPLN 248 + GP YETPAEI M+R G DAVGMSTV E IVARHAG++VLGISCI+NMAAGILDQPL+ Sbjct: 186 MTGPVYETPAEIRMIRTVGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGILDQPLH 245 Query: 249 HAEVMETADRVREHFLKLVLALIPKM 274 H EV+ET +RV+ +FL LV A++ +M Sbjct: 246 HDEVIETTERVKANFLTLVKAIVKQM 271 >A9VFJ1.1 A9VFJ1_BACWK Purine nucleoside phosphorylase I, inosine and guanosine-specific Length = 273 Score = 331 bits (849), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 160/266 (60%), Positives = 198/266 (74%) Query: 9 IQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTVEGHEGELL 68 I ++A Y+K+K P+ AD I + V +PY EIP FPVSTVEGH G+L+ Sbjct: 6 ITKSASYLKEKFQETPQVGLILGSGLGVLADEIENAVTVPYSEIPEFPVSTVEGHAGQLV 65 Query: 69 IGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTDFTPGDL 128 G ++G VV M+GRFH YEGY + FPVRVMKELGV +++VTNAAGGVNT F PGDL Sbjct: 66 FGTLQGVTVVAMQGRFHFYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTSFEPGDL 125 Query: 129 MLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFKEGVYAG 188 MLI+DH+N G NPL GPND+ +GVRFPDMST+Y+P L +AK+ AA + +EGVY G Sbjct: 126 MLISDHINFMGTNPLIGPNDSEMGVRFPDMSTSYTPELREMAKQVAADLNIKVQEGVYVG 185 Query: 189 LLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGILDQPLN 248 + GP YETPAEI MLR G DAVGMSTV E IVARHAG++VLGISCI+NMAAGILDQPL+ Sbjct: 186 MTGPVYETPAEIRMLRTLGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGILDQPLH 245 Query: 249 HAEVMETADRVREHFLKLVLALIPKM 274 H EV+ET +RV+ +FL LV A++ +M Sbjct: 246 HDEVIETTERVKANFLALVKAIVKQM 271 >C3AAD0.1 C3AAD0_BACMY Purine nucleoside phosphorylase Length = 273 Score = 331 bits (849), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 160/266 (60%), Positives = 198/266 (74%) Query: 9 IQEAAVYIKDKCPVAPEXXXXXXXXXXXXADLITDEVVIPYHEIPHFPVSTVEGHEGELL 68 I ++A Y+K+K P+ AD I + V +PY EIP FPVSTVEGH G+L+ Sbjct: 6 ITKSASYLKEKFQETPQVGLILGSGLGVLADEIENAVTVPYSEIPEFPVSTVEGHAGQLV 65 Query: 69 IGMIEGRRVVMMKGRFHMYEGYGPETTAFPVRVMKELGVASLLVTNAAGGVNTDFTPGDL 128 G ++G VV M+GRFH YEGY + FPVRVMKELGV +++VTNAAGGVNT F PGDL Sbjct: 66 FGTLQGVTVVAMQGRFHFYEGYDMQKVTFPVRVMKELGVETVVVTNAAGGVNTSFEPGDL 125 Query: 129 MLITDHLNLTGRNPLTGPNDNALGVRFPDMSTAYSPRLIAVAKEAAAAQGFEFKEGVYAG 188 MLI+DH+N G NPL GPND+ +GVRFPDMST+Y+P L +AK+ AA + +EGVY G Sbjct: 126 MLISDHINFMGTNPLIGPNDSEMGVRFPDMSTSYTPELREMAKQVAADLNIKVQEGVYVG 185 Query: 189 LLGPCYETPAEIVMLRRQGADAVGMSTVSETIVARHAGIEVLGISCITNMAAGILDQPLN 248 + GP YETPAEI MLR G DAVGMSTV E IVARHAG++VLGISCI+NMAAGILDQPL+ Sbjct: 186 MTGPVYETPAEIRMLRTLGGDAVGMSTVPEVIVARHAGMKVLGISCISNMAAGILDQPLH 245 Query: 249 HAEVMETADRVREHFLKLVLALIPKM 274 H EV+ET +RV+ +FL LV A++ +M Sbjct: 246 HDEVIETTERVKANFLALVKAIVKQM 271 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,525,248,721 Number of extensions: 61478969 Number of successful extensions: 136444 Number of sequences better than 1.0e-05: 1085 Number of HSP's gapped: 135682 Number of HSP's successfully gapped: 1088 Length of query: 274 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 140 Effective length of database: 1,798,280,193 Effective search space: 251759227020 Effective search space used: 251759227020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)