BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0010.p (240 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK69.1 B3KK69_9BACI Lipoate-protein ligase B 327 5e-88 B1DJD5.1 B1DJD5_9BACL Lipoate-protein ligase B 272 2e-71 A9AV27.1 A9AV27_HERA2 Lipoate-protein ligase B 223 1e-56 Q1AT14.1 LIPB_RUBXD Octanoyltransferase EC=2.3.1.181 192 2e-47 C0UUD7.1 C0UUD7_9BACT Lipoate-protein ligase B 186 1e-45 >B3KK69.1 B3KK69_9BACI Lipoate-protein ligase B Length = 227 Score = 327 bits (838), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 154/219 (70%), Positives = 180/219 (82%) Query: 9 LEISYLPLIEYGAAWELQKAAVQAIDAGTGPERLILLQHPPTYTIGSQHHPEHLLLSAEQ 68 L++SY P+++YG AWELQK V AID G E L+LLQHPPTYT+GSQ HPEHLLLS E+ Sbjct: 5 LDVSYTPIVDYGMAWELQKNHVSAIDQGERHESLMLLQHPPTYTMGSQRHPEHLLLSKEE 64 Query: 69 LKEKGIALFEIDRGGDITYHGPGQLVGYPLLKLGMDGKVDLHGYLRRLEAVIIDFLASYG 128 L+++GI LFEIDRGGDITYHGPGQLVGYPLL L ++LHG+LR +E VIID+LASY Sbjct: 65 LEKQGIGLFEIDRGGDITYHGPGQLVGYPLLLLDGGKNLNLHGFLRDVEQVIIDYLASYD 124 Query: 129 IEGSRKPEYTGVWVGDQKICAIGVKFNKSKHRRGFITSHGFAFNVTAGIGERGFQGIIPC 188 IE RKPEYTGVWVGD KICAIGVKFNK + RGF+TSHGFAFN+ +GI + GF GIIPC Sbjct: 125 IEAGRKPEYTGVWVGDSKICAIGVKFNKCRRHRGFVTSHGFAFNIKSGIADAGFSGIIPC 184 Query: 189 GIAEYGVTSLEECAGRSFELGEVARELVPCFLKHFPYEA 227 GI EYGVTSLE+C G+ FE+ EVARE+VP F + FPYEA Sbjct: 185 GIQEYGVTSLEDCTGQMFEVEEVAREIVPFFTRVFPYEA 223 >B1DJD5.1 B1DJD5_9BACL Lipoate-protein ligase B Length = 220 Score = 272 bits (695), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 129/217 (59%), Positives = 163/217 (75%), Gaps = 1/217 (0%) Query: 16 LIEYGAAWELQKAAVQAIDAGTGPERLILLQHPPTYTIGSQHHPEHLLLSAEQLKEKGIA 75 +I Y AWE QK+ V+ I E L+LLQHPPTYT+G+ H EHLLLS ++L E+GI+ Sbjct: 1 MIGYAEAWETQKSIVRQISQEERQETLLLLQHPPTYTLGTDRHHEHLLLSEDELNERGIS 60 Query: 76 LFEIDRGGDITYHGPGQLVGYPLLKLGMDGKVDLHGYLRRLEAVIIDFLASYGIEGSRKP 135 +FEIDRGGDITYHGPGQLVGYP+L L M+G +DLH YLR +E II++LA YGIE RKP Sbjct: 61 VFEIDRGGDITYHGPGQLVGYPMLFLPMEG-LDLHAYLRNVEQAIINWLAEYGIEAGRKP 119 Query: 136 EYTGVWVGDQKICAIGVKFNKSKHRRGFITSHGFAFNVTAGIGERGFQGIIPCGIAEYGV 195 EYTGVWVG++KI AIGVKFN+++ R GF+TSHGFA N+ AGIG GF GI+PCGI EYGV Sbjct: 120 EYTGVWVGNEKIAAIGVKFNRARDRGGFVTSHGFALNIEAGIGNEGFSGIVPCGIQEYGV 179 Query: 196 TSLEECAGRSFELGEVARELVPCFLKHFPYEAAAAVE 232 TS + +G + + E AR+L+P F + F + +E Sbjct: 180 TSFNDLSGVTLSVEEAARQLLPHFCRVFGFAVPNLLE 216 >A9AV27.1 A9AV27_HERA2 Lipoate-protein ligase B Length = 224 Score = 223 bits (567), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 117/216 (54%), Positives = 148/216 (68%), Gaps = 8/216 (3%) Query: 8 LLEISYLPLIEYGAAWELQKAAVQAIDAGTGPERLILLQHPPTYTIGSQHHPEHLLLSAE 67 LL++ L +++Y AAWELQ+ V A T P+ L+LL+HP TYTIG EHLL+S E Sbjct: 9 LLKVQILGMLDYNAAWELQRELVSQRSAETIPDTLVLLEHPATYTIGRSGGYEHLLVSPE 68 Query: 68 QLKEKGIALFEIDRGGDITYHGPGQLVGYPLLKLGMDGKVDLHGYLRRLEAVIIDFLASY 127 L+++G AL E+DRGGDITYHGPGQLVGYP+L++ G+ DLH YLR LEAVII+ LA Y Sbjct: 69 TLQQRGAALIEVDRGGDITYHGPGQLVGYPILQIRDHGR-DLHQYLRMLEAVIIELLAEY 127 Query: 128 GIEGSRKPEYTGVWVGDQKICAIGVKFNKSKHRRGFITSHGFAFNVTAGIGERGFQGIIP 187 G+ R P YTGVWVGD+KI AIGV+ N RG +TSHGFA NVT + + F I+P Sbjct: 128 GLSARRFPGYTGVWVGDRKIAAIGVRSNT----RG-VTSHGFALNVTTNLAD--FTAIVP 180 Query: 188 CGIAEYGVTSLEECAGRSFELGEVARELVPCFLKHF 223 CGI +YGVTSL E +V + L+ FL+ F Sbjct: 181 CGIHDYGVTSLAELLPVVPAFNDVQQRLIRIFLRVF 216 >Q1AT14.1 LIPB_RUBXD Octanoyltransferase EC=2.3.1.181 Length = 221 Score = 192 bits (487), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 107/213 (50%), Positives = 131/213 (61%), Gaps = 12/213 (5%) Query: 9 LEISYLP-LIEYGAAWELQKAAVQAIDAGTGPERLILLQHPPTYTIG-SQHHPEHLLLSA 66 L++ LP L Y AWELQ+ V+ +G P+ L+LL+HPP YT+G + +L Sbjct: 10 LDVRRLPGLTPYAEAWELQRELVRKRRSGEIPDTLLLLEHPPVYTVGRAARDASNLGAGE 69 Query: 67 EQLKEKGIALFEIDRGGDITYHGPGQLVGYPLLKLGMDGKVDLHGYLRRLEAVIIDFLAS 126 E L+ G +F DRGGD T+HGPGQLVGYP+++L + D H YLR LE V+I LA Sbjct: 70 EYLRSLGAEVFWSDRGGDATFHGPGQLVGYPIIRLKVR---DTHRYLRDLEEVVIRALAG 126 Query: 127 YGIEGSRKPEYTGVWVGDQKICAIGVKFNKSKHRRGFITSHGFAFNVTAGIGERGFQGII 186 YG+EG R P YTGVWV KICAIGVKF+ G+I SHGFA NV + F I Sbjct: 127 YGLEGRRHPRYTGVWVNGSKICAIGVKFSS-----GWIASHGFALNVNTDLS--WFDRIT 179 Query: 187 PCGIAEYGVTSLEECAGRSFELGEVARELVPCF 219 PCGI EYGVTSLE GR L V RE+V F Sbjct: 180 PCGIREYGVTSLERELGREIPLAGVEREIVSGF 212 >C0UUD7.1 C0UUD7_9BACT Lipoate-protein ligase B Length = 207 Score = 186 bits (472), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 102/208 (49%), Positives = 126/208 (60%), Gaps = 9/208 (4%) Query: 16 LIEYGAAWELQKAAVQAIDAGTGPERLILLQHPPTYTIGSQHHPEHLLLSAEQLKEKGIA 75 ++ Y AWELQK+ V +I + PE LILLQHP TYTIG + +HLL S EQL+ + Sbjct: 3 IVPYMEAWELQKSLVASIASNEHPESLILLQHPHTYTIGRKGGHDHLLASIEQLRAMNVE 62 Query: 76 LFEIDRGGDITYHGPGQLVGYPLLKLGMDGKVDLHGYLRRLEAVIIDFLASYGIEGSRKP 135 + E DRGGDITYHGP Q+VGYPL+ L G D+H YLR LE V+I L YG R Sbjct: 63 VIETDRGGDITYHGPEQIVGYPLINLRNIGS-DVHKYLRMLEEVLIRTLNDYGFSAHRDE 121 Query: 136 EYTGVWVGDQKICAIGVKFNKSKHRRGFITSHGFAFNVTAGIGERGFQGIIPCGIAEYGV 195 +YTGVW+G KI AIGVK ++ IT HGFA N+ + R F IIPCGI V Sbjct: 122 QYTGVWIGKAKIAAIGVKISRG------ITMHGFAININTDL--RYFDMIIPCGITGRSV 173 Query: 196 TSLEECAGRSFELGEVARELVPCFLKHF 223 TSL+ GR+ L EV + F + F Sbjct: 174 TSLQVLLGRTIPLEEVMDHIEHHFSEVF 201 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.141 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,551,087,526 Number of extensions: 67898903 Number of successful extensions: 146284 Number of sequences better than 1.0e-05: 1050 Number of HSP's gapped: 143550 Number of HSP's successfully gapped: 1051 Length of query: 240 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 108 Effective length of database: 1,817,122,223 Effective search space: 196249200084 Effective search space used: 196249200084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)