BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0009.p (276 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK68.1 B3KK68_9BACI Catalytic domain of component of various d... 475 e-132 B1DJD6.1 B1DJD6_9BACL Catalytic domain of components of various ... 410 e-113 C0ZC39.1 C0ZC39_BREBN Lipoamide acyltransferase component of bra... 341 4e-92 B4BLG4.1 B4BLG4_9BACI Catalytic domain of component of various d... 321 4e-86 A4IQQ0.1 A4IQQ0_GEOTN Dihydrolipoamide acetyltransferase 321 5e-86 >B3KK68.1 B3KK68_9BACI Catalytic domain of component of various dehydrogenase complexes Length = 470 Score = 475 bits (1223), Expect = e-132, Method: Compositional matrix adjust. Identities = 223/261 (85%), Positives = 247/261 (94%) Query: 16 ALEPVRHSGLHLSETPRIPTIEVEGGRGSSSEYLIDVTPIRNTIATRMRQSVSEIPHAWT 75 A+ VRHSGLHL+E+P+IPTIEVEG G SEY IDVTP+RN IA MRQSV+EIPHAWT Sbjct: 210 AIPDVRHSGLHLTESPKIPTIEVEGMEGGRSEYFIDVTPMRNAIARNMRQSVTEIPHAWT 269 Query: 76 MIEVDVTNLVILRNKLKDEFKRKEGINLTYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVK 135 MIEVDVTN+V+LRN+LKDEFKRKEG+NLTYLAFL+KAVV+AIKDYPIMNS WAVDKI+VK Sbjct: 270 MIEVDVTNIVLLRNQLKDEFKRKEGVNLTYLAFLLKAVVNAIKDYPIMNSFWAVDKIVVK 329 Query: 136 RDINISLAVGTEDSVMTPVIKKADQKNVAGLAREIDELAMKTREGKLRLEDMQGGTFTVN 195 RDIN+SLAVGTEDSV+TPVI +ADQKN+AGLAREI++LA KTREGKL+L+DMQGGTFTVN Sbjct: 330 RDINLSLAVGTEDSVLTPVIHRADQKNIAGLAREIEDLARKTREGKLKLDDMQGGTFTVN 389 Query: 196 NTGSFGSILSYPIINYPQAAILTFESIVKKPVVINDMIAVRSMANICLSLDHRILDGVIC 255 NTGSFGSILSYPIINYPQAAILTFESIVKKPVVINDMIAVRSMAN+CLSLDHRILDGVIC Sbjct: 390 NTGSFGSILSYPIINYPQAAILTFESIVKKPVVINDMIAVRSMANLCLSLDHRILDGVIC 449 Query: 256 GRFLQRIKDNVEGYTPDTKLY 276 GRFLQR+KDN+EGYT DTKLY Sbjct: 450 GRFLQRVKDNLEGYTLDTKLY 470 >B1DJD6.1 B1DJD6_9BACL Catalytic domain of components of various dehydrogenase complexes Length = 436 Score = 410 bits (1054), Expect = e-113, Method: Compositional matrix adjust. Identities = 198/261 (75%), Positives = 226/261 (86%), Gaps = 7/261 (2%) Query: 20 VRHSGLHLSETPRIPTIEVEG----GRGSSSEYLIDVTPIRNTIATRMRQSVSEIPHAWT 75 VR SGLHL+ETPR PT+ + GRG E IDV+PIR+ IA SV+EIPH W Sbjct: 179 VRSSGLHLTETPRTPTVPSQSTSIPGRG---EDFIDVSPIRSAIARNTMLSVNEIPHGWM 235 Query: 76 MIEVDVTNLVILRNKLKDEFKRKEGINLTYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVK 135 MIEVDVTNLV LRNK+KDEF ++EGINLTYLAF++KAVV+A+KD+PIMNS+WAVDKIIVK Sbjct: 236 MIEVDVTNLVQLRNKVKDEFMKREGINLTYLAFVLKAVVNALKDFPIMNSMWAVDKIIVK 295 Query: 136 RDINISLAVGTEDSVMTPVIKKADQKNVAGLAREIDELAMKTREGKLRLEDMQGGTFTVN 195 RDINISLAVGTE+SV TPVIK AD K +AGLAREID+LA + REGKL L DMQGGTFTVN Sbjct: 296 RDINISLAVGTEESVATPVIKNADHKTIAGLAREIDDLARRGREGKLTLSDMQGGTFTVN 355 Query: 196 NTGSFGSILSYPIINYPQAAILTFESIVKKPVVINDMIAVRSMANICLSLDHRILDGVIC 255 NTGSFGSI++ PIINYPQAAILTFESIVK+PVVINDMIA+RSMAN+CLSLDHR+LDGVIC Sbjct: 356 NTGSFGSIITNPIINYPQAAILTFESIVKRPVVINDMIAIRSMANMCLSLDHRVLDGVIC 415 Query: 256 GRFLQRIKDNVEGYTPDTKLY 276 GRFLQR+KDN+EG+ DTKLY Sbjct: 416 GRFLQRVKDNLEGFNVDTKLY 436 >C0ZC39.1 C0ZC39_BREBN Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex EC=2.3.1.168 Length = 445 Score = 341 bits (875), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 155/233 (66%), Positives = 193/233 (82%) Query: 44 SSSEYLIDVTPIRNTIATRMRQSVSEIPHAWTMIEVDVTNLVILRNKLKDEFKRKEGINL 103 +S + ++ VT IR TIA RM QS E PHAWTM+EVDVTNLV RN+ K EF RKEG+NL Sbjct: 213 ASGDQVVPVTSIRRTIANRMVQSKHEAPHAWTMVEVDVTNLVNFRNQAKGEFARKEGLNL 272 Query: 104 TYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVKRDINISLAVGTEDSVMTPVIKKADQKNV 163 T+L F +KAVV A+K++P++NS WA DKIIVK+DINIS+AV TE+++ PVIK ADQK++ Sbjct: 273 TFLPFFIKAVVEALKEFPMINSTWAHDKIIVKKDINISIAVATEEALYVPVIKHADQKSI 332 Query: 164 AGLAREIDELAMKTREGKLRLEDMQGGTFTVNNTGSFGSILSYPIINYPQAAILTFESIV 223 G+A+ +D+LA +TR GKL ++DM GGTFTVNNTGSFGS+LS PIIN PQAAIL+ ESIV Sbjct: 333 LGIAKAVDDLAARTRAGKLTMDDMTGGTFTVNNTGSFGSVLSQPIINAPQAAILSVESIV 392 Query: 224 KKPVVINDMIAVRSMANICLSLDHRILDGVICGRFLQRIKDNVEGYTPDTKLY 276 K+PVVINDMIAVRSM N+C+SLDHR+LDG++CGRFLQ +K +E PDTKLY Sbjct: 393 KRPVVINDMIAVRSMVNLCMSLDHRVLDGLVCGRFLQSVKQKLENVGPDTKLY 445 >B4BLG4.1 B4BLG4_9BACI Catalytic domain of component of various dehydrogenase complexes Length = 441 Score = 321 bits (823), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 155/250 (62%), Positives = 190/250 (76%), Gaps = 6/250 (2%) Query: 28 SETPRIPTIEVEGGRGSSSEYLIDVTPIRNTIATRMRQSVSEIPHAWTMIEVDVTNLVIL 87 S P +PTIEV G + I VTP+R IA M +S E PHAWTM+EVDVTNLV Sbjct: 197 STKPAVPTIEVGAG-----DIEIPVTPVRKAIAANMLRSKHEAPHAWTMVEVDVTNLVAY 251 Query: 88 RNKLKDEFKRKEGINLTYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVKRDINISLAVGTE 147 R+ +KDEFKR+EG NLTY AF +KAV A+K++P +NSVWA DKII ++DINIS+AV T+ Sbjct: 252 RDAIKDEFKRREGFNLTYFAFFVKAVAQALKEFPQLNSVWAGDKIIQRKDINISIAVATD 311 Query: 148 DSVMTPVIKKADQKNVAGLAREIDELAMKTREGKLRLEDMQGGTFTVNNTGSFGSILSYP 207 D++ PVIK AD+K + G+AREI ELA KTR GKLR EDMQGGTFTVNNTG+FGS+ S Sbjct: 312 DALFVPVIKHADEKTIKGIAREIAELAAKTRAGKLRPEDMQGGTFTVNNTGAFGSVQSMG 371 Query: 208 IINYPQAAILTFESIVKKPVVINDMIAVRSMANICLSLDHRILDGVICGRFLQRIKDNVE 267 IINYPQAAIL E+IVK+PVV + MIA+R M N+CLSLDHR+LDG++CGRFL R+K +E Sbjct: 372 IINYPQAAILQVETIVKRPVVKDGMIAIRDMVNLCLSLDHRVLDGLVCGRFLARVKAILE 431 Query: 268 GYTPD-TKLY 276 D T +Y Sbjct: 432 NMNKDNTPVY 441 >A4IQQ0.1 A4IQQ0_GEOTN Dihydrolipoamide acetyltransferase Length = 434 Score = 321 bits (822), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 155/250 (62%), Positives = 190/250 (76%), Gaps = 6/250 (2%) Query: 28 SETPRIPTIEVEGGRGSSSEYLIDVTPIRNTIATRMRQSVSEIPHAWTMIEVDVTNLVIL 87 S P +PTIEV G + I VTP+R IA M +S E PHAWTM+EVDVTNLV Sbjct: 190 STKPAVPTIEVGAG-----DIEIPVTPVRKAIAANMLRSKHEAPHAWTMVEVDVTNLVAY 244 Query: 88 RNKLKDEFKRKEGINLTYLAFLMKAVVSAIKDYPIMNSVWAVDKIIVKRDINISLAVGTE 147 R+ +KDEFKR+EG NLTY AF +KAV A+K++P +NSVWA DKII ++DINIS+AV T+ Sbjct: 245 RDAIKDEFKRREGFNLTYFAFFVKAVAQALKEFPQLNSVWAGDKIIQRKDINISIAVATD 304 Query: 148 DSVMTPVIKKADQKNVAGLAREIDELAMKTREGKLRLEDMQGGTFTVNNTGSFGSILSYP 207 D++ PVIK AD+K + G+AREI ELA KTR GKLR EDMQGGTFTVNNTG+FGS+ S Sbjct: 305 DALFVPVIKHADEKTIKGIAREIAELAAKTRAGKLRPEDMQGGTFTVNNTGAFGSVQSMG 364 Query: 208 IINYPQAAILTFESIVKKPVVINDMIAVRSMANICLSLDHRILDGVICGRFLQRIKDNVE 267 IINYPQAAIL E+IVK+PVV + MIA+R M N+CLSLDHR+LDG++CGRFL R+K +E Sbjct: 365 IINYPQAAILQVETIVKRPVVKDGMIAIRDMVNLCLSLDHRVLDGLVCGRFLARVKAILE 424 Query: 268 GYTPD-TKLY 276 D T +Y Sbjct: 425 NMNKDNTPVY 434 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,536,186,430 Number of extensions: 59221545 Number of successful extensions: 160026 Number of sequences better than 1.0e-05: 3574 Number of HSP's gapped: 156320 Number of HSP's successfully gapped: 3575 Length of query: 276 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 142 Effective length of database: 1,798,280,193 Effective search space: 255355787406 Effective search space used: 255355787406 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)