BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0005.p (260 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK67.1 B3KK67_9BACI Transketolase central region 417 e-115 B1DJD7.1 B1DJD7_9BACL Transketolase central region 395 e-108 P37941.1 ODBB_BACSU 2-oxoisovalerate dehydrogenase subunit beta ... 362 2e-98 A7Z6G7.1 A7Z6G7_BACA2 BkdAB EC=1.2.4.4 361 4e-98 B1HRV8.1 B1HRV8_LYSSC 2-oxoisovalerate dehydrogenase subunit bet... 360 5e-98 >B3KK67.1 B3KK67_9BACI Transketolase central region Length = 328 Score = 417 bits (1073), Expect = e-115, Method: Compositional matrix adjust. Identities = 211/243 (86%), Positives = 222/243 (91%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 MA+MEYIDAIRLAMKEEMERDESVFVLGED L +QFGE RV+DTPLAE Sbjct: 1 MAVMEYIDAIRLAMKEEMERDESVFVLGEDVGVKGGVFTTTKGLMDQFGEMRVLDTPLAE 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 SAIAGVAIGAAMYGMKPIAEMQYSDFM PATNQIISEAAKIRYRSNNDWSCPVVIRAPIG Sbjct: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMFPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 GGIFGGLYHSQCPES+FFGTPGLKIVAPYSAYDAKGLLKAA+RDPDPVLFFENKKCYKLI Sbjct: 121 GGIFGGLYHSQCPESVFFGTPGLKIVAPYSAYDAKGLLKAAIRDPDPVLFFENKKCYKLI 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 DVP+ DY VPIG+ANLLREGSDITVI YSLPLHFAMQAAEELE+EEGI+AH+LDLRT+ Sbjct: 181 TGDVPDDDYVVPIGKANLLREGSDITVISYSLPLHFAMQAAEELEKEEGISAHVLDLRTI 240 Query: 241 QPL 243 QPL Sbjct: 241 QPL 243 >B1DJD7.1 B1DJD7_9BACL Transketolase central region Length = 327 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 204/251 (81%), Positives = 219/251 (87%), Gaps = 1/251 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 MA M+YIDAIRLAMKEEMERD+ VFVLGED L EQFGE R +DTPLAE Sbjct: 1 MAKMDYIDAIRLAMKEEMERDDDVFVLGEDVGVKGGVFTTTKGLLEQFGEMRALDTPLAE 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 SAIAGVAIGAAMYGMKPIAEMQYSDFM PATNQIISEAAKIRYRSNNDWSCP+VIRAPIG Sbjct: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMFPATNQIISEAAKIRYRSNNDWSCPLVIRAPIG 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 GGIFGGLYHSQCPES+FFGTPG+KIVAPY+AYDAKGLLKAAVRDPDPV+FFENKKCYKLI Sbjct: 121 GGIFGGLYHSQCPESVFFGTPGIKIVAPYTAYDAKGLLKAAVRDPDPVIFFENKKCYKLI 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 DVPE DY V IG+AN+LREG DITVIGYS+PL F QAA EL +EGI+ HILDLRTL Sbjct: 181 NGDVPEDDYVVEIGKANVLREGDDITVIGYSMPLMFVEQAAAELA-QEGISTHILDLRTL 239 Query: 241 QPLDREAIIAA 251 QPLD+EAI+AA Sbjct: 240 QPLDKEAILAA 250 >P37941.1 ODBB_BACSU 2-oxoisovalerate dehydrogenase subunit beta EC=1.2.4.4 Length = 327 Score = 362 bits (928), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 176/251 (70%), Positives = 205/251 (81%), Gaps = 1/251 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 M++M YIDAI LAMKEEMERD VFVLGED L EQFGEERVMDTPLAE Sbjct: 1 MSVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAE 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 SAIAGV IGAAMYGM+PIAEMQ++DF++PA NQIISEAAKIRYRSNNDWSCP+V+RAP G Sbjct: 61 SAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIISEAAKIRYRSNNDWSCPIVVRAPYG 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 GG+ G LYHSQ E+IF PGLKIV P + YDAKGLLKAAVRD DPVLFFE+K+ Y+LI Sbjct: 121 GGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLI 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 K +VP DY +PIG+A++ REG DITVI Y L +HFA+QAAE LE+ +GI+AH++DLRT+ Sbjct: 181 KGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAERLEK-DGISAHVVDLRTV 239 Query: 241 QPLDREAIIAA 251 PLD+EAII A Sbjct: 240 YPLDKEAIIEA 250 >A7Z6G7.1 A7Z6G7_BACA2 BkdAB EC=1.2.4.4 Length = 327 Score = 361 bits (926), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 178/251 (70%), Positives = 205/251 (81%), Gaps = 1/251 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 M++M YID+I AMKEEMERD VFVLGED L EQFGEERVMDTPLAE Sbjct: 1 MSVMSYIDSINAAMKEEMERDPRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAE 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 SAIAGV IGAAMYGM+PIAEMQ++DF+LPA NQIISEAAKIRYRSNNDWSCP+VIRAP G Sbjct: 61 SAIAGVGIGAAMYGMRPIAEMQFADFILPAVNQIISEAAKIRYRSNNDWSCPMVIRAPYG 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 GG+ G LYHSQ E+IF PGLKIV P + YDAKGLLKAAVRD DPVLFFE+K+ Y+LI Sbjct: 121 GGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLI 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 K +VP DY +PIG+A++ REGSDITVI Y L +HFA+QAAE LE+ +GI+AH+LDLRT+ Sbjct: 181 KGEVPADDYVLPIGKADVKREGSDITVITYGLCVHFALQAAERLEK-DGISAHVLDLRTV 239 Query: 241 QPLDREAIIAA 251 PLD+EAII A Sbjct: 240 YPLDKEAIIEA 250 >B1HRV8.1 B1HRV8_LYSSC 2-oxoisovalerate dehydrogenase subunit beta EC=1.2.4.4 Length = 327 Score = 360 bits (925), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 175/251 (69%), Positives = 206/251 (82%), Gaps = 1/251 (0%) Query: 1 MAIMEYIDAIRLAMKEEMERDESVFVLGEDXXXXXXXXXXXXXLQEQFGEERVMDTPLAE 60 MA+M YIDAI LAMKEEMERDE VF+LGED L +QFGE RV+DTPLAE Sbjct: 1 MAVMSYIDAITLAMKEEMERDERVFILGEDVGRKGGVFKATTGLYDQFGEYRVLDTPLAE 60 Query: 61 SAIAGVAIGAAMYGMKPIAEMQYSDFMLPATNQIISEAAKIRYRSNNDWSCPVVIRAPIG 120 SAIAGV IGAAMYGM+PIAEMQ++DF++PA NQI+SEAAKIRYRSNNDW+CP+VIRAP G Sbjct: 61 SAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIVSEAAKIRYRSNNDWTCPMVIRAPFG 120 Query: 121 GGIFGGLYHSQCPESIFFGTPGLKIVAPYSAYDAKGLLKAAVRDPDPVLFFENKKCYKLI 180 GGI G LYHSQ E++F GTPGLKIV P + YDAKGLLKAA+RD DPVLFFE+K+ Y+LI Sbjct: 121 GGIHGALYHSQSVEALFAGTPGLKIVIPSTPYDAKGLLKAAIRDEDPVLFFEHKRAYRLI 180 Query: 181 KEDVPEGDYTVPIGEANLLREGSDITVIGYSLPLHFAMQAAEELEREEGITAHILDLRTL 240 K +VP DYT+PIG+A++ REG D+TVI Y L +HFA+QAAE L +GI+AHILDLRT+ Sbjct: 181 KGEVPLDDYTLPIGKADVKREGDDVTVITYGLAVHFALQAAERLA-ADGISAHILDLRTV 239 Query: 241 QPLDREAIIAA 251 PLD+EAII A Sbjct: 240 YPLDKEAIIEA 250 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,443,460,611 Number of extensions: 59460991 Number of successful extensions: 137451 Number of sequences better than 1.0e-05: 2710 Number of HSP's gapped: 133782 Number of HSP's successfully gapped: 2714 Length of query: 260 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 127 Effective length of database: 1,807,701,208 Effective search space: 229578053416 Effective search space used: 229578053416 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)