BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0004.p (343 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK66.1 B3KK66_9BACI 3-methyl-2-oxobutanoate dehydrogenase (2-m... 556 e-156 B1DJD8.1 B1DJD8_9BACL 3-methyl-2-oxobutanoate dehydrogenase (2-m... 531 e-149 C3J0M8.1 C3J0M8_9BACI 3-methyl-2-oxobutanoate dehydrogenase (2-m... 431 e-119 B7YCI3.1 B7YCI3_9BACI 3-methyl-2-oxobutanoate dehydrogenase (2-m... 431 e-119 Q5KXC3.1 Q5KXC3_GEOKA Branched-chain alpha-keto acid dehydrogena... 429 e-118 >B3KK66.1 B3KK66_9BACI 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) EC=1.2.4.4 Length = 342 Score = 556 bits (1433), Expect = e-156, Method: Compositional matrix adjust. Identities = 265/342 (77%), Positives = 291/342 (85%), Gaps = 2/342 (0%) Query: 2 ESQGTVETINRHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXX 61 E + NRH+ LGL+DGQV+DMY+ M L RK+DER+LLLQRAGKINFHVSGIGQE Sbjct: 3 EHETVTANNNRHEQLGLTDGQVLDMYKYMVLARKFDERNLLLQRAGKINFHVSGIGQEAC 62 Query: 62 XXXXXXXLDRENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSK 121 LDRE DYFLPYYRDY FVL+VGMT RELMLS FAKAEDPNSGGRQMPGHFGSK Sbjct: 63 QVAAAFALDREKDYFLPYYRDYGFVLAVGMTPRELMLSAFAKAEDPNSGGRQMPGHFGSK 122 Query: 122 RLRIVTGSSPVTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKL 181 RLRIVTGSSPVTTQVPHAVG ALAAKMQKK FVSFVTFGEGSSNQGDFHE CNFAGV+KL Sbjct: 123 RLRIVTGSSPVTTQVPHAVGIALAAKMQKKDFVSFVTFGEGSSNQGDFHEGCNFAGVHKL 182 Query: 182 PVIIFCQNNQYAISVPAHKQLGGKVSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALA 241 PVII C+NNQYAISVP HKQ+ GKVSDRALGYGFPGVRVDGND LEVY VKEARERA+ Sbjct: 183 PVIIMCENNQYAISVPLHKQISGKVSDRALGYGFPGVRVDGNDALEVYAAVKEARERAIR 242 Query: 242 GEGPTLIEAMMYRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQES 301 GEGPTLIE+MMYRLSPHSTSDNDLAYRTKEEV+ NW KDG+A F++YL + GLW + ++ Sbjct: 243 GEGPTLIESMMYRLSPHSTSDNDLAYRTKEEVEENWKKDGVARFKNYLIDCGLWDEARDQ 302 Query: 302 DLAAEYNLELKAAIEYAENAPFPKPEDTLLHVYSESDLKGGA 343 DL A+ NLE+K A E A+ APFPKPEDTLLHVY +D +GGA Sbjct: 303 DLIAQLNLEIKEATESADLAPFPKPEDTLLHVY--ADAEGGA 342 >B1DJD8.1 B1DJD8_9BACL 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) EC=1.2.4.4 Length = 337 Score = 531 bits (1368), Expect = e-149, Method: Compositional matrix adjust. Identities = 253/336 (75%), Positives = 282/336 (83%), Gaps = 1/336 (0%) Query: 3 SQGTVETINRHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXX 62 SQ T +T RH LGLS+ Q ++MY M L RK+DER+LLLQRAGKINFHVSGIGQE Sbjct: 2 SQSTEQTF-RHNELGLSNEQAVEMYAMMMLARKFDERNLLLQRAGKINFHVSGIGQEVAQ 60 Query: 63 XXXXXXLDRENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKR 122 LDRENDYFLPYYRDY FVL+VGMT +ELMLSVFAKAEDPNSGGRQMPGHFGSKR Sbjct: 61 VAAAFALDRENDYFLPYYRDYGFVLAVGMTVKELMLSVFAKAEDPNSGGRQMPGHFGSKR 120 Query: 123 LRIVTGSSPVTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLP 182 LRIVTGSSPVTTQVPHAVG ALAAKM+ K VSFVTFGEGSSNQGDFHE CNFAGV+KLP Sbjct: 121 LRIVTGSSPVTTQVPHAVGIALAAKMKNKPIVSFVTFGEGSSNQGDFHEGCNFAGVHKLP 180 Query: 183 VIIFCQNNQYAISVPAHKQLGGKVSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAG 242 VI+ C+NNQYAISVP HKQLGG+V+DRALGYGFPG +VDGND LEV+RVVKEARERA+AG Sbjct: 181 VILMCENNQYAISVPVHKQLGGRVADRALGYGFPGYQVDGNDALEVFRVVKEARERAIAG 240 Query: 243 EGPTLIEAMMYRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQESD 302 EGPTLIEAMMYRLSPHSTSDNDLAYRTKEEVD N +KDGI +++YL E GLW++E E++ Sbjct: 241 EGPTLIEAMMYRLSPHSTSDNDLAYRTKEEVDENRSKDGIPKYKTYLEECGLWNEELEAE 300 Query: 303 LAAEYNLELKAAIEYAENAPFPKPEDTLLHVYSESD 338 L A + A EY +NAPF PE LLHVY+E + Sbjct: 301 LLARIRKMVDEATEYGDNAPFAAPESVLLHVYAEEE 336 >C3J0M8.1 C3J0M8_9BACI 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) EC=1.2.4.4 Length = 331 Score = 431 bits (1108), Expect = e-119, Method: Compositional matrix adjust. Identities = 213/327 (65%), Positives = 246/327 (75%), Gaps = 1/327 (0%) Query: 11 NRHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXXXLD 70 NRH+ LGLSD V++MY TM L RK DER LL RAGKI F +S GQE LD Sbjct: 4 NRHQALGLSDDTVLEMYETMLLARKLDERMWLLNRAGKIPFVISCQGQEAAQVGAAFALD 63 Query: 71 RENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVTGSS 130 R DY LPYYRD VL+ GMT RELML+ FAKAEDPNSGGRQMPGHFG K+ RIVTGSS Sbjct: 64 RTKDYVLPYYRDVGVVLTFGMTPRELMLAAFAKAEDPNSGGRQMPGHFGKKKNRIVTGSS 123 Query: 131 PVTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFCQNN 190 PVTTQVPHAVGFALAAKM+KK FV+FVTFGEGSSNQGDFHE NFAGV+KLPVI C+NN Sbjct: 124 PVTTQVPHAVGFALAAKMEKKDFVAFVTFGEGSSNQGDFHEGANFAGVHKLPVIFMCENN 183 Query: 191 QYAISVPAHKQLGG-KVSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPTLIE 249 +YAISVP KQL KVSDRA+GYG PG VDG DPLEVYRVVKEA +RA GEGPTLIE Sbjct: 184 KYAISVPISKQLACEKVSDRAIGYGMPGYTVDGTDPLEVYRVVKEAADRARRGEGPTLIE 243 Query: 250 AMMYRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQESDLAAEYNL 309 A+ YRL+ HS+ D+ YRT+EE+ AKD I +F +YL E G+ +DEQ+ ++ A Sbjct: 244 AVTYRLTSHSSDDDHRVYRTEEELAEARAKDPIVSFANYLKETGVLTDEQDEEIQARVMK 303 Query: 310 ELKAAIEYAENAPFPKPEDTLLHVYSE 336 E+ A +YAE AP+ +PE L +VY+E Sbjct: 304 EVNEATDYAEKAPYAEPEHALRYVYAE 330 >B7YCI3.1 B7YCI3_9BACI 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) EC=1.2.4.4 Length = 331 Score = 431 bits (1108), Expect = e-119, Method: Compositional matrix adjust. Identities = 213/327 (65%), Positives = 246/327 (75%), Gaps = 1/327 (0%) Query: 11 NRHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXXXLD 70 NRH+ LGLSD V++MY TM L RK DER LL RAGKI F +S GQE LD Sbjct: 4 NRHQALGLSDDTVLEMYETMLLARKLDERMWLLNRAGKIPFVISCQGQEAAQVGAAFALD 63 Query: 71 RENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVTGSS 130 R DY LPYYRD VL+ GMT RELML+ FAKAEDPNSGGRQMPGHFG K+ RIVTGSS Sbjct: 64 RTKDYVLPYYRDVGVVLTFGMTPRELMLAAFAKAEDPNSGGRQMPGHFGKKKNRIVTGSS 123 Query: 131 PVTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFCQNN 190 PVTTQVPHAVGFALAAKM+KK FV+FVTFGEGSSNQGDFHE NFAGV+KLPVI C+NN Sbjct: 124 PVTTQVPHAVGFALAAKMEKKDFVAFVTFGEGSSNQGDFHEGANFAGVHKLPVIFMCENN 183 Query: 191 QYAISVPAHKQLGG-KVSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPTLIE 249 +YAISVP KQL KVSDRA+GYG PG VDG DPLEVYRVVKEA +RA GEGPTLIE Sbjct: 184 KYAISVPISKQLACEKVSDRAIGYGMPGYTVDGTDPLEVYRVVKEAADRARRGEGPTLIE 243 Query: 250 AMMYRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQESDLAAEYNL 309 A+ YRL+ HS+ D+ YRT+EE+ AKD I +F +YL E G+ +DEQ+ ++ A Sbjct: 244 AVTYRLTSHSSDDDHRVYRTEEELAEARAKDPIVSFANYLKETGVLTDEQDEEIQARVMK 303 Query: 310 ELKAAIEYAENAPFPKPEDTLLHVYSE 336 E+ A +YAE AP+ +PE L +VY+E Sbjct: 304 EVNEATDYAEKAPYAEPEHALRYVYAE 330 >Q5KXC3.1 Q5KXC3_GEOKA Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain (2-oxoisovalerate dehydrogenase alpha subunit) EC=1.2.4.4 Length = 331 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 212/327 (64%), Positives = 245/327 (74%), Gaps = 1/327 (0%) Query: 11 NRHKPLGLSDGQVIDMYRTMQLGRKYDERSLLLQRAGKINFHVSGIGQEXXXXXXXXXLD 70 NRH+ LGLSD V++MY TM L RK DER LL RAGKI F +S GQE LD Sbjct: 4 NRHQALGLSDDTVLEMYETMLLARKLDERMWLLNRAGKIPFVISCQGQEAAQVGAAFALD 63 Query: 71 RENDYFLPYYRDYAFVLSVGMTTRELMLSVFAKAEDPNSGGRQMPGHFGSKRLRIVTGSS 130 R DY LPYYRD VL+ GMT RELML+ FAKAEDPNSGGRQMPGHFG K+ RIVTGSS Sbjct: 64 RTKDYVLPYYRDVGVVLTFGMTPRELMLAAFAKAEDPNSGGRQMPGHFGKKKNRIVTGSS 123 Query: 131 PVTTQVPHAVGFALAAKMQKKKFVSFVTFGEGSSNQGDFHEACNFAGVNKLPVIIFCQNN 190 PVTTQVPHAVGFALAAKM+KK FV+FVTFGEGSSNQGDFHE NFAGV+KLPVI C+NN Sbjct: 124 PVTTQVPHAVGFALAAKMEKKDFVAFVTFGEGSSNQGDFHEGANFAGVHKLPVIFMCENN 183 Query: 191 QYAISVPAHKQLGG-KVSDRALGYGFPGVRVDGNDPLEVYRVVKEARERALAGEGPTLIE 249 +YAISVP KQL KVSDRA+GYG PG VDG DPLEVYRVVKEA +RA GEGPTLIE Sbjct: 184 KYAISVPISKQLACEKVSDRAIGYGMPGYTVDGTDPLEVYRVVKEAADRARRGEGPTLIE 243 Query: 250 AMMYRLSPHSTSDNDLAYRTKEEVDANWAKDGIAAFRSYLTELGLWSDEQESDLAAEYNL 309 A+ YRL+ HS+ D+ YRT+EE+ AKD I +F +YL E G+ +D+ + D+ A Sbjct: 244 AVTYRLTSHSSDDDHRVYRTEEELAEARAKDPIVSFANYLKETGVLTDQLDEDIQARVMK 303 Query: 310 ELKAAIEYAENAPFPKPEDTLLHVYSE 336 E+ A +YAE AP+ +PE L +VY+E Sbjct: 304 EVNEATDYAEKAPYAEPEHALRYVYAE 330 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,088,882,573 Number of extensions: 87061082 Number of successful extensions: 182382 Number of sequences better than 1.0e-05: 2177 Number of HSP's gapped: 177859 Number of HSP's successfully gapped: 2180 Length of query: 343 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 207 Effective length of database: 1,779,438,163 Effective search space: 368343699741 Effective search space used: 368343699741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)