BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0003.p (224 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK65.1 B3KK65_9BACI Dihydrolipoyl dehydrogenase EC=1.8.1.4 318 2e-85 P54533.1 DLDH2_BACSU Dihydrolipoyl dehydrogenase EC=1.8.1.4 298 2e-79 Q65HK7.1 Q65HK7_BACLD Dihydrolipoyl dehydrogenase EC=1.8.1.4 292 1e-77 B7GHH6.1 B7GHH6_ANOFW Dihydrolipoyl dehydrogenase EC=1.8.1.4 289 1e-76 B1DJD9.1 B1DJD9_9BACL Dihydrolipoyl dehydrogenase EC=1.8.1.4 287 5e-76 >B3KK65.1 B3KK65_9BACI Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 473 Score = 318 bits (815), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 152/223 (68%), Positives = 182/223 (81%) Query: 2 ITESEVTIEARKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTN 61 I V+I + T++LSA+KLL+SVGRV N ENIGLENTD+ G+I VN NMQT Sbjct: 251 IRNQSVSIAIEHKDDTRTLSADKLLLSVGRVGNTENIGLENTDVRVENGIIAVNGNMQTT 310 Query: 62 EPHIYAIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYT 121 EPHIYAIGDCIGGLQLAHAASHEGI AV+HLAGE + +PRC+YTR EVASVG T Sbjct: 311 EPHIYAIGDCIGGLQLAHAASHEGIAAVNHLAGEKTESFRSEWIPRCIYTRHEVASVGLT 370 Query: 122 EKEARALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIG 181 EK+A+ G ++ G+FPFSAIGK++++G K+GFVKV+AD+ + DILGV M+GPHVT+LIG Sbjct: 371 EKQAKERGHEIKIGRFPFSAIGKSLIHGTKEGFVKVIADQKTQDILGVHMMGPHVTELIG 430 Query: 182 EAALAQLLDATPWEIGEAVHAHPTLSEIIGEAMLAVDGRAIGF 224 EAALAQ+LDATPWE+G AVHAHP+LSEIIGEAMLAVDGRAIG Sbjct: 431 EAALAQVLDATPWEVGTAVHAHPSLSEIIGEAMLAVDGRAIGL 473 >P54533.1 DLDH2_BACSU Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 474 Score = 298 bits (763), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 146/222 (65%), Positives = 173/222 (77%) Query: 3 TESEVTIEARKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTNE 62 T +++I+A K +T + SAEK+LVS+GR ANIE IGLENTDI G+I VN++ QT E Sbjct: 253 TSDDISIQAEKDGETVTYSAEKMLVSIGRQANIEGIGLENTDIVTENGMISVNESCQTKE 312 Query: 63 PHIYAIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYTE 122 HIYAIGD IGGLQLAH ASHEGI AV+H AG HP P LVP+C+Y+ PE ASVG TE Sbjct: 313 SHIYAIGDVIGGLQLAHVASHEGIIAVEHFAGLNPHPLDPTLVPKCIYSSPEAASVGLTE 372 Query: 123 KEARALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIGE 182 EA+A G +V GKFPF AIGKA+VYG DGFVK+VADR + DILGV MIGPHVTD+I E Sbjct: 373 DEAKANGHNVKIGKFPFMAIGKALVYGESDGFVKIVADRDTDDILGVHMIGPHVTDMISE 432 Query: 183 AALAQLLDATPWEIGEAVHAHPTLSEIIGEAMLAVDGRAIGF 224 A LA++LDATPWE+G+ +H HPTLSE IGEA LA DG+AI F Sbjct: 433 AGLAKVLDATPWEVGQTIHPHPTLSEAIGEAALAADGKAIHF 474 >Q65HK7.1 Q65HK7_BACLD Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 473 Score = 292 bits (748), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 146/218 (66%), Positives = 166/218 (76%) Query: 7 VTIEARKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTNEPHIY 66 V+I+A K + QS SAEK+LVSVGR ANIE IGLENTDI G I N QT E HIY Sbjct: 256 VSIQAEKDGEKQSYSAEKMLVSVGRQANIEGIGLENTDIQVENGFIVTNSMYQTKESHIY 315 Query: 67 AIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYTEKEAR 126 AIGD IGGLQLAH ASHEGI AV+H+AGE H LV +C+Y+ PE ASVG TE EA+ Sbjct: 316 AIGDVIGGLQLAHVASHEGITAVEHIAGENPHAIDYSLVSKCIYSSPEAASVGLTEGEAK 375 Query: 127 ALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIGEAALA 186 A G V GKFPF AIGKA+VYG DGFVK+VADR + DILGV MIGPHVTD+I EA LA Sbjct: 376 AQGKSVKVGKFPFQAIGKALVYGETDGFVKIVADRETDDILGVHMIGPHVTDMISEAGLA 435 Query: 187 QLLDATPWEIGEAVHAHPTLSEIIGEAMLAVDGRAIGF 224 ++LDATPWE+G+ +H HPTLSE IGEA LAVDG+AI F Sbjct: 436 KVLDATPWEVGQTIHPHPTLSEAIGEAALAVDGKAIHF 473 >B7GHH6.1 B7GHH6_ANOFW Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 474 Score = 289 bits (740), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 140/224 (62%), Positives = 173/224 (77%) Query: 1 VITESEVTIEARKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQT 60 ++ E+ V I+A + ++ +AEK+LVSVGR AN+E IGLENTDI KG I+ N+ QT Sbjct: 251 LVVENGVRIQAEINGKNETFTAEKMLVSVGRQANVEGIGLENTDIVVEKGFIQTNEYYQT 310 Query: 61 NEPHIYAIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGY 120 E HIYAIGD IGGLQLAH ASHEGI A++HLAG+ +HP +VP+C+Y+RPE ASVG Sbjct: 311 KEKHIYAIGDVIGGLQLAHVASHEGIIAIEHLAGKEVHPLDYAMVPKCIYSRPEAASVGL 370 Query: 121 TEKEARALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLI 180 TE+EARA G DV GKFPF AIGKA+VYG +GFVK+VAD + D+LGV M+GPHVTD+I Sbjct: 371 TEEEARAKGYDVKVGKFPFKAIGKALVYGEAEGFVKIVADAKTDDLLGVHMVGPHVTDMI 430 Query: 181 GEAALAQLLDATPWEIGEAVHAHPTLSEIIGEAMLAVDGRAIGF 224 EA LA++LDATPWEI +H HPTLSE + EA LAVDG+AI F Sbjct: 431 SEAGLARVLDATPWEIAHTIHPHPTLSEAMMEAALAVDGKAIHF 474 >B1DJD9.1 B1DJD9_9BACL Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 475 Score = 287 bits (735), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 140/223 (62%), Positives = 170/223 (76%) Query: 2 ITESEVTIEARKGEQTQSLSAEKLLVSVGRVANIENIGLENTDIHFGKGVIEVNDNMQTN 61 I EV+I A E L+A K+L+SVGR ANIENIGLENTDI GVI VN+ QT Sbjct: 253 INGDEVSITAETSEGNILLTASKVLLSVGRQANIENIGLENTDIQTKNGVIYVNEFGQTT 312 Query: 62 EPHIYAIGDCIGGLQLAHAASHEGIRAVDHLAGETLHPYHPHLVPRCVYTRPEVASVGYT 121 EPHIYAIGD IGG+QLAHAASHEG+ A +H+AG P+L+PRC+Y+RPEVAS G T Sbjct: 313 EPHIYAIGDVIGGVQLAHAASHEGVAAAEHIAGHKKAAVDPNLIPRCIYSRPEVASYGLT 372 Query: 122 EKEARALGADVVTGKFPFSAIGKAIVYGMKDGFVKVVADRSSGDILGVQMIGPHVTDLIG 181 E AR+ G D+ TGK PF+AIGKA+V G DGFVKV+ADR + DI+GV MIGPH TDLI Sbjct: 373 EDGARSKGHDIKTGKIPFAAIGKALVLGEADGFVKVIADRKTNDIIGVHMIGPHATDLIS 432 Query: 182 EAALAQLLDATPWEIGEAVHAHPTLSEIIGEAMLAVDGRAIGF 224 EA+LA LL+ATPWE+G+ +H HPTLSE +GEAMLA+DG+++ Sbjct: 433 EASLAGLLNATPWEVGQLIHPHPTLSEALGEAMLAIDGKSLAL 475 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,292,865,541 Number of extensions: 53857750 Number of successful extensions: 231072 Number of sequences better than 1.0e-05: 5364 Number of HSP's gapped: 223545 Number of HSP's successfully gapped: 5364 Length of query: 224 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 93 Effective length of database: 1,826,543,238 Effective search space: 169868521134 Effective search space used: 169868521134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)