BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0002.p (221 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KK65.1 B3KK65_9BACI Dihydrolipoyl dehydrogenase EC=1.8.1.4 309 1e-82 B1DJD9.1 B1DJD9_9BACL Dihydrolipoyl dehydrogenase EC=1.8.1.4 271 3e-71 C5D454.1 C5D454_9BACI Dihydrolipoamide dehydrogenase 242 2e-62 B7GHH6.1 B7GHH6_ANOFW Dihydrolipoyl dehydrogenase EC=1.8.1.4 238 2e-61 C3J0M9.1 C3J0M9_9BACI Dihydrolipoamide dehydrogenase 238 4e-61 >B3KK65.1 B3KK65_9BACI Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 473 Score = 309 bits (792), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 152/217 (70%), Positives = 174/217 (80%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M I+CDVAILGGGTGGYVAAIRAAQLGK+VV+IE DKLGGTCLHRGCIPSK+LLRSAE+Y Sbjct: 1 MAITCDVAILGGGTGGYVAAIRAAQLGKNVVIIEQDKLGGTCLHRGCIPSKALLRSAELY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 AE+++SESYGIET GV+L F KVQ RK+ +VE+LHQGVQ+LMRKNKI V++GKGR+IGPS Sbjct: 61 AEMKDSESYGIETDGVRLSFNKVQKRKDGIVEKLHQGVQYLMRKNKITVVQGKGRVIGPS 120 Query: 121 IFSPRSGAVAVELENGEMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXXX 180 IFSPRSGAVAVEL +GEMETVV +LI+ATGSRPR LPGL+PDG I Sbjct: 121 IFSPRSGAVAVELPDGEMETVVPTHLIIATGSRPRHLPGLEPDGIHILTSDHALQLDELP 180 Query: 181 XXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGVQVT+VEA+ LLP Sbjct: 181 SSIIIVGGGVIGVEWASMLNDFGVQVTIVEASSHLLP 217 >B1DJD9.1 B1DJD9_9BACL Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 475 Score = 271 bits (693), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 138/223 (61%), Positives = 161/223 (72%), Gaps = 2/223 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 MTI DVA+LGGG GGY AAIRAAQLGKSV ++E+DKLGGTCLHRGCIPSKSLLRSAEVY Sbjct: 1 MTIQVDVAVLGGGPGGYTAAIRAAQLGKSVAIVELDKLGGTCLHRGCIPSKSLLRSAEVY 60 Query: 61 AEIQESESYGIETA--GVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIG 118 A + E++ YG+ + L F KVQ RKE VEQLH+G++ LM+K IQ++ GKGR+IG Sbjct: 61 ATLLEADKYGVSVTEGALSLDFNKVQDRKEKTVEQLHRGLKGLMKKYDIQIINGKGRVIG 120 Query: 119 PSIFSPRSGAVAVELENGEMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXX 178 PSIFSPRSG++AVEL +GEME+VVS NLI+ATGSRPR LPGLKPDG I Sbjct: 121 PSIFSPRSGSLAVELADGEMESVVSTNLIIATGSRPRHLPGLKPDGVHILSSDDALQLEK 180 Query: 179 XXXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLPXXXA 221 EWASML DFGVQVT+VE +LLP A Sbjct: 181 LPESILIVGGGVIGVEWASMLQDFGVQVTLVEVGARLLPGEDA 223 >C5D454.1 C5D454_9BACI Dihydrolipoamide dehydrogenase Length = 473 Score = 242 bits (618), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 122/218 (55%), Positives = 151/218 (69%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M D+ ILGGGTGGYVAAIRA+QLG V+E KLGGTCLH GCIPSK+LLRSAEVY Sbjct: 1 MAKEYDLVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 A+ + SE++G+ + V+L F KVQARK A++EQLH+GVQ LM+K KI V G GRI+GPS Sbjct: 61 AQTKNSETFGVMASDVRLDFAKVQARKAAIIEQLHKGVQHLMKKGKIDVYAGTGRILGPS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G ++VE+ +G E E +V N+I+ATGSRPR LPGL DGK++ Sbjct: 121 IFSPMPGTISVEMNDGSENEMLVPKNVIIATGSRPRTLPGLDIDGKLVMTSDEALQMETL 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGV+VTV+E A ++LP Sbjct: 181 PSSIIIVGGGVIGIEWASMLNDFGVEVTVLEYAERILP 218 >B7GHH6.1 B7GHH6_ANOFW Dihydrolipoyl dehydrogenase EC=1.8.1.4 Length = 474 Score = 238 bits (608), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 119/218 (54%), Positives = 150/218 (68%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M DV ILGGGTGGYVAAIRAAQLG V+E KLGGTCLH GCIPSK+LLRSAEVY Sbjct: 2 MAKEYDVVILGGGTGGYVAAIRAAQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVY 61 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 A+ +ESE++G+ + V+L F KVQARK+A+++QLH+G+ LM+K KI V +G GRI+GPS Sbjct: 62 AQTKESETFGVIASEVRLDFSKVQARKQAIIDQLHKGIHMLMKKGKIDVYEGTGRILGPS 121 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G ++VE+ +G E E +V +++ATGSRPR LPGL DG+ + Sbjct: 122 IFSPMPGTISVEMNDGTENEMLVPKYVVIATGSRPRTLPGLTIDGQFVMTSDEALRMETL 181 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGV VTV+E A ++LP Sbjct: 182 PSSIIIVGGGVIGIEWASMLNDFGVDVTVIEYADRILP 219 >C3J0M9.1 C3J0M9_9BACI Dihydrolipoamide dehydrogenase Length = 473 Score = 238 bits (606), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 122/218 (55%), Positives = 149/218 (68%), Gaps = 1/218 (0%) Query: 1 MTISCDVAILGGGTGGYVAAIRAAQLGKSVVVIEMDKLGGTCLHRGCIPSKSLLRSAEVY 60 M DV ILGGGTGGYVAAIRA+QLG V+E KLGGTCLH GCIPSK+LLRSAEVY Sbjct: 1 MAKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVY 60 Query: 61 AEIQESESYGIETAGVQLVFPKVQARKEAVVEQLHQGVQFLMRKNKIQVLKGKGRIIGPS 120 A+ + E++G+ GV+L F KVQARK A+VEQLH+GVQ LM+K KI V G GR++GPS Sbjct: 61 AQTKNGEAFGVIADGVRLDFAKVQARKAAIVEQLHKGVQHLMKKGKIDVYAGTGRLLGPS 120 Query: 121 IFSPRSGAVAVELENG-EMETVVSGNLIVATGSRPRVLPGLKPDGKVIXXXXXXXXXXXX 179 IFSP G V+VE+ +G E E +V +++ATGSRPR LPGL+PDG+ + Sbjct: 121 IFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPGLEPDGEFVMTSDEALQMETL 180 Query: 180 XXXXXXXXXXXXXXEWASMLADFGVQVTVVEAAGQLLP 217 EWASML DFGV VTV+E A ++LP Sbjct: 181 PSSILIVGGGVIGMEWASMLNDFGVDVTVLEYADRILP 218 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,064,076,880 Number of extensions: 37713409 Number of successful extensions: 162696 Number of sequences better than 1.0e-05: 5001 Number of HSP's gapped: 154687 Number of HSP's successfully gapped: 5001 Length of query: 221 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 90 Effective length of database: 1,826,543,238 Effective search space: 164388891420 Effective search space used: 164388891420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)