BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0019.p (221 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K741.1 B3K741_9BACI Lipolytic protein G-D-S-L family 273 7e-72 B1DJS9.1 B1DJS9_9BACL Lipolytic protein G-D-S-L family 253 1e-65 C4CS88.1 C4CS88_9SPHI Lysophospholipase L1-like esterase 201 3e-50 A6DGF5.1 A6DGF5_9BACT Probable lipase/esterase 172 2e-41 B4D7D9.1 B4D7D9_9BACT Lipolytic protein G-D-S-L family 168 3e-40 >B3K741.1 B3K741_9BACI Lipolytic protein G-D-S-L family Length = 218 Score = 273 bits (699), Expect = 7e-72, Method: Compositional matrix adjust. Identities = 125/215 (58%), Positives = 153/215 (71%) Query: 4 FSGKSKVFLFQGDSITDGNRGRDEDPNHILGHSYAYIIGGRLGNELAEQKPVFYNRGISG 63 + K LFQGDSITDGN GR++DPNHILGH Y Y+I +LG E A+ +P+F NRG+SG Sbjct: 1 MENQRKTILFQGDSITDGNWGRNQDPNHILGHGYVYLIAAQLGLEFAQSQPLFINRGVSG 60 Query: 64 DRISDLYARWNEDAIYLKPDVLSILIGVNDLWRIMKGEPSGVTDRFERAYRHVLVETREV 123 +R+S+LYARWNEDA LKPD++SIL GVND RI++G+P G TDRFER YR +L ETREV Sbjct: 61 NRVSELYARWNEDAFSLKPDLISILAGVNDAGRIIQGQPEGATDRFERIYRQLLSETREV 120 Query: 124 LPQTKLVLCEPFILRTGAPAEAWDEWSKHMAHYQQVVRALAEEFGALHVPLQXXXXXXXX 183 LP+T LVLCEPF+L+T A A WD+W + Y +VR LAEE+ L VPLQ Sbjct: 121 LPETGLVLCEPFVLQTAATANGWDQWRSKIDEYGSIVRQLAEEYHTLFVPLQEVFNQEAQ 180 Query: 184 XXXXXYWLWDGVHPTHAGHDLIANEWLSAAGKSGI 218 YWLWDGVHPT AGH L+A EWL +S + Sbjct: 181 NVTAEYWLWDGVHPTAAGHQLLAREWLKTVQQSDL 215 >B1DJS9.1 B1DJS9_9BACL Lipolytic protein G-D-S-L family Length = 215 Score = 253 bits (645), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 127/208 (61%), Positives = 144/208 (69%) Query: 9 KVFLFQGDSITDGNRGRDEDPNHILGHSYAYIIGGRLGNELAEQKPVFYNRGISGDRISD 68 KV LFQGDSITD R R+ +LG YAY I GRLGNE AE F NRGISG+R+SD Sbjct: 3 KVILFQGDSITDCGRSREGYAGQLLGQGYAYHIAGRLGNEQAEAGHQFINRGISGNRVSD 62 Query: 69 LYARWNEDAIYLKPDVLSILIGVNDLWRIMKGEPSGVTDRFERAYRHVLVETREVLPQTK 128 LYARWNEDAI L+PDVLSILIGVND WRIM PSG TDRFERAYR +L ET+EVLP T Sbjct: 63 LYARWNEDAIALRPDVLSILIGVNDTWRIMDKLPSGATDRFERAYRVLLEETKEVLPDTG 122 Query: 129 LVLCEPFILRTGAPAEAWDEWSKHMAHYQQVVRALAEEFGALHVPLQXXXXXXXXXXXXX 188 LVLCEPF+LRTG A W W + + +VV+ LAEE+ A+ VP Q Sbjct: 123 LVLCEPFVLRTGVTARDWSGWRERVERQSEVVKQLAEEYHAVFVPFQQTFDQAAEKAEPD 182 Query: 189 YWLWDGVHPTHAGHDLIANEWLSAAGKS 216 YWL+DGVHPT AGH L+A+EWL A S Sbjct: 183 YWLFDGVHPTAAGHYLMASEWLKAVQSS 210 >C4CS88.1 C4CS88_9SPHI Lysophospholipase L1-like esterase Length = 264 Score = 201 bits (512), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 98/207 (47%), Positives = 126/207 (60%), Gaps = 1/207 (0%) Query: 10 VFLFQGDSITDGNRGRDEDPNHILGHSYAYIIGGRLGNELAEQKPVFYNRGISGDRISDL 69 VFLFQGDSITDGNRGR+ DPNHI+GH YAY I R+G + E+ VFYNRGISG++I+DL Sbjct: 46 VFLFQGDSITDGNRGRNTDPNHIMGHGYAYAIASRIGADFPERDFVFYNRGISGNKITDL 105 Query: 70 YARWNEDAIYLKPDVLSILIGVNDL-WRIMKGEPSGVTDRFERAYRHVLVETREVLPQTK 128 RW D + LKPDVLSILIG+ND I K + ++FE YR +L + R P+T Sbjct: 106 QKRWQPDTLALKPDVLSILIGINDTGATINKPAEATTPEQFETIYRSLLDDCRRANPETL 165 Query: 129 LVLCEPFILRTGAPAEAWDEWSKHMAHYQQVVRALAEEFGALHVPLQXXXXXXXXXXXXX 188 VL PF+ G+ + W+ W + Q V+R LA EF A+ V Sbjct: 166 FVLGIPFVYPVGSRQDNWERWQSDTRNRQAVIRKLATEFNAVVVDYPAVLDQAIAQKNTE 225 Query: 189 YWLWDGVHPTHAGHDLIANEWLSAAGK 215 YW+WDG+HPT H+L+A EW+ K Sbjct: 226 YWVWDGIHPTVFAHELMAREWIKQVSK 252 >A6DGF5.1 A6DGF5_9BACT Probable lipase/esterase Length = 697 Score = 172 bits (437), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 90/217 (41%), Positives = 131/217 (60%), Gaps = 10/217 (4%) Query: 1 MSTFSGKSKVFLFQGDSITDGNRGRDE-DPNHILGHSYAYIIGGRLGNELAEQKPVFYNR 59 + F S+V LF GDSITD NRGR+E D NH LGHS+ ++I G+LG +L + + FYNR Sbjct: 327 LPAFKKGSRV-LFIGDSITDMNRGRNERDRNHYLGHSFVFLIAGKLGVDLPKAQLDFYNR 385 Query: 60 GISGDRISDLYARWNEDAIYLKPDVLSILIGVNDLWRIMKGEPSGVTDRFERAYRHVLVE 119 GISG+ I+ L ARW +D I ++PD+L+ILIG ND+ + +K FE YR +L Sbjct: 386 GISGNTIAQLKARWQKDVIAMRPDLLTILIGTNDVGKKVKPH------DFENDYRTILSA 439 Query: 120 TREVLPQTKLVLCEPFILRTGA--PAEAWDEWSKHMAHYQQVVRALAEEFGALHVPLQXX 177 +R++ P+ K+VL +PF+L++G+ +W+ + +V LA++F A+H+ Q Sbjct: 440 SRKMNPKLKIVLLDPFVLQSGSLKNERSWNSRRSATDQLRPIVAKLAKDFDAVHIKTQDL 499 Query: 178 XXXXXXXXXXXYWLWDGVHPTHAGHDLIANEWLSAAG 214 YWLWDG+HP GH+LI WL A Sbjct: 500 FDTAAQSSSPDYWLWDGIHPLPQGHELITRHWLRAVS 536 >B4D7D9.1 B4D7D9_9BACT Lipolytic protein G-D-S-L family Length = 240 Score = 168 bits (425), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 89/209 (42%), Positives = 122/209 (58%), Gaps = 7/209 (3%) Query: 1 MSTFSGKSKVFLFQGDSITDGNRGRDEDPNHILGHSYAYIIGGRLGNELAEQKPVFYNRG 60 ++ FS ++V LFQGDSITDGNRGR+ DPNHILGH Y +II + G A F NRG Sbjct: 28 IAAFSSGTRV-LFQGDSITDGNRGRNADPNHILGHGYVFIIAAKYGAAFAALDLDFANRG 86 Query: 61 ISGDRISDLYARWNEDAIYLKPDVLSILIGVNDLWRIMKGEPSGVTDRFERAYRHVLVET 120 +SG+ + +L RW +D + +KPDVLSILIGVND + D +ER Y ++ + Sbjct: 87 VSGNTVRELQKRWEKDTLEMKPDVLSILIGVNDQSHDVP------IDEYERTYDELITQA 140 Query: 121 REVLPQTKLVLCEPFILRTGAPAEAWDEWSKHMAHYQQVVRALAEEFGALHVPLQXXXXX 180 + P+ ++VLCEPF L G + + W + Q VV LA + A V Q Sbjct: 141 KTANPKLRIVLCEPFTLPVGKRKDGYAAWRAGIQARQDVVARLAAKHDAALVRFQPVFDA 200 Query: 181 XXXXXXXXYWLWDGVHPTHAGHDLIANEW 209 +W+WDGVHPT++GH L+A+EW Sbjct: 201 ACKIAPAEHWIWDGVHPTYSGHQLMADEW 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,408,886,046 Number of extensions: 57122858 Number of successful extensions: 126522 Number of sequences better than 1.0e-05: 71 Number of HSP's gapped: 126414 Number of HSP's successfully gapped: 71 Length of query: 221 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 90 Effective length of database: 1,826,543,238 Effective search space: 164388891420 Effective search space used: 164388891420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)