BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0014.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5P1.1 B3K5P1_9BACI Binding-protein-dependent transport system... 458 e-127 B1D7Q3.1 B1D7Q3_9BACL Binding-protein-dependent transport system... 389 e-106 B3KEQ7.1 B3KEQ7_9BACI Binding-protein-dependent transport system... 375 e-102 B1DAC3.1 B1DAC3_9BACL Binding-protein-dependent transport system... 185 4e-45 Q2BFH7.1 Q2BFH7_9BACI Sugar ABC transporter permease 177 1e-42 >B3K5P1.1 B3K5P1_9BACI Binding-protein-dependent transport systems inner membrane component Length = 300 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 217/298 (72%), Positives = 256/298 (85%) Query: 1 MNKSKNAAFAYSFLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRILRD 60 MNK+K+ AF+YSFLLPS +LT+VLGIYPI WA RYMFY+YKG+G RF+GL NF R+++D Sbjct: 1 MNKAKSVAFSYSFLLPSFILTMVLGIYPIMWAMRYMFYDYKGFGAERFVGLANFERVVKD 60 Query: 61 TEFWNSVVNTFVYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVV 120 +FW SV NTF+YAGGK N +I+GR LLRA+FFMPT+ISTAVMAVV Sbjct: 61 HQFWESVANTFIYAGGKLLLSIPLALILAVILNWRIKGRNLLRAIFFMPTIISTAVMAVV 120 Query: 121 FFTIFNSYNGILNQFLIKFNISSSGIDWLGPKHAMLTVILVAVWGAVGNYMLLFLAGLQN 180 FFTIFNSYNGILNQFL+K+ I + IDWLG +AMLTVILVAVWGA+GNYMLLFLAGLQN Sbjct: 121 FFTIFNSYNGILNQFLLKYQIIGNAIDWLGVDYAMLTVILVAVWGAIGNYMLLFLAGLQN 180 Query: 181 IPEDVYESSSLDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLTEGGPAG 240 IPEDVYESS+LDGA+K QQFR+IT+PMLGPV+QM+IMLAIINALKGYESIMV+T+GGP G Sbjct: 181 IPEDVYESSALDGASKFQQFRYITIPMLGPVMQMIIMLAIINALKGYESIMVMTDGGPIG 240 Query: 241 KTEVMFLYLYKLFFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASRRMNR 298 KT VMFLY+YKLFFP GS ASTQ+QEFGYGSAVAFV+AVIVG+I+++YFY S+RM+ Sbjct: 241 KTNVMFLYVYKLFFPPAGSTASTQIQEFGYGSAVAFVTAVIVGVITILYFYGSKRMSH 298 >B1D7Q3.1 B1D7Q3_9BACL Binding-protein-dependent transport systems inner membrane component Length = 298 Score = 389 bits (999), Expect = e-106, Method: Compositional matrix adjust. Identities = 186/299 (62%), Positives = 239/299 (79%), Gaps = 2/299 (0%) Query: 1 MNKSKNAAFAYSFLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRILRD 60 M + +YSF+LPS + T+VLGIYPI WA RYMFY+Y+GYG FIG NF+ ++ D Sbjct: 1 MTSLRKLGESYSFILPSLVFTVVLGIYPILWAIRYMFYDYQGYGEEVFIGFGNFANLMSD 60 Query: 61 TEFWNSVVNTFVYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVV 120 +W+SVVNT VYA GK N+ ++G+ LRA++FMPTV+STAV+++V Sbjct: 61 GAYWSSVVNTAVYAMGKIIITIPLSLILAVILNKGLKGKNFLRAIYFMPTVLSTAVISIV 120 Query: 121 FFTIFNSYNGILNQFLIKFNISSSGIDWLGPKHAMLTVILVAVWGAVGNYMLLFLAGLQN 180 FF IFNSYNGILNQFL+K++I S+ IDWLGP AM T I+VA+WGA+GNYMLLFLAGLQN Sbjct: 121 FFNIFNSYNGILNQFLLKYHIISNSIDWLGPDLAMATSIIVAIWGAIGNYMLLFLAGLQN 180 Query: 181 IPEDVYESSSLDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLTEGGPAG 240 IP+DVYES+SLDGAN Q+F +IT+PMLGPVLQM+IMLAI +LKGYESIMVLTEGGP G Sbjct: 181 IPDDVYESASLDGANASQKFFYITIPMLGPVLQMIIMLAITISLKGYESIMVLTEGGPIG 240 Query: 241 KTEVMFLYLYKLFFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASRRMNRI 299 +TEVM+LY+Y++FFP+ S++ST +Q+ GYGSAV F+SAVIVG+I++ YF+ S+R+NRI Sbjct: 241 RTEVMYLYVYRMFFPI--SSSSTMIQQIGYGSAVGFMSAVIVGLITVGYFWLSKRLNRI 297 >B3KEQ7.1 B3KEQ7_9BACI Binding-protein-dependent transport systems inner membrane component Length = 299 Score = 375 bits (962), Expect = e-102, Method: Compositional matrix adjust. Identities = 184/287 (64%), Positives = 224/287 (78%), Gaps = 2/287 (0%) Query: 13 FLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRILRDTEFWNSVVNTFV 72 FL PS +LT+ LGIYP+ W RYMFY+Y GYG A F+GLDNF R++RD FW SV NTF+ Sbjct: 14 FLSPSIILTVALGIYPLLWMLRYMFYDYAGYGDALFVGLDNFRRLMRDALFWESVGNTFI 73 Query: 73 YAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVVFFTIFNSYNGIL 132 YAGGK N K+RG LLR ++FMPT+ISTAV++VVF+TIFNSYNG++ Sbjct: 74 YAGGKLLLTLPLSLLLAVILNGKLRGGNLLRGIYFMPTIISTAVISVVFYTIFNSYNGMV 133 Query: 133 NQFLIKFNISSSGIDWLGPKHAMLTVILVAVWGAVGNYMLLFLAGLQNIPEDVYESSSLD 192 N L+K ++ S IDWLGP HAMLTVILVAVWGAVGNYMLLFLAGLQ+IP+D+YES+++D Sbjct: 134 NTVLMKLHLVSQPIDWLGPNHAMLTVILVAVWGAVGNYMLLFLAGLQSIPQDLYESAAMD 193 Query: 193 GANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLTEGGPAGKTEVMFLYLYKL 252 GAN ++F ITLPML PV Q+VIMLAII +LKGYESIM++TEGGP GKTEVM+LYLYKL Sbjct: 194 GANAGRRFWSITLPMLAPVAQLVIMLAIIASLKGYESIMLITEGGPIGKTEVMYLYLYKL 253 Query: 253 FFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASRRMNRI 299 FPV S S Q+ GYGSAV F +AVIVG I+ +YF SR+MN++ Sbjct: 254 LFPV--STGSPVEQQLGYGSAVGFATAVIVGAITGLYFLLSRKMNKL 298 >B1DAC3.1 B1DAC3_9BACL Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 185 bits (470), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 102/292 (34%), Positives = 165/292 (56%), Gaps = 11/292 (3%) Query: 5 KNAAFAYSFLLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRILRDTEFW 64 +N Y+FLLP+ L L+ +P+ +F F + G+G +F G N++R+ D F Sbjct: 8 RNRLTGYTFLLPNILGFLIFICFPVCASFLISFTEWNGFGEIKFTGFSNYARLWSDDTFR 67 Query: 65 NSVVNTFVYAGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVVFFTI 124 S++N+ V NR I+G +L R F+P + +T +AVV+ + Sbjct: 68 ISLINSLVMTAVSVPVTLMLAILAAVALNRGIKGVKLFRTAIFLPHITATIAVAVVWQLL 127 Query: 125 FNSYNGILNQFLIKFNISSSGIDWLGPKH-AMLTVILVAVWGAVGNYMLLFLAGLQNIPE 183 +N G +N FL I+ WL A+++VI+V++W ++G YM+L+LAGLQ IP+ Sbjct: 128 YNPTMGPINGFLRSLGIAHPP-AWLASTQWALISVIIVSIWHSIGYYMVLYLAGLQGIPK 186 Query: 184 DVYESSSLDGANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLTEGGPAGKTE 243 D+YE++ +DGA K+ QFR ITLPML PV+ +++ IIN+ K ++ + VLT+GGP T Sbjct: 187 DLYEAAEIDGAGKVSQFRNITLPMLSPVIFFTVIMGIINSFKVFDLVYVLTKGGPGRSTH 246 Query: 244 VMFLYLYKLFFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASRR 295 ++ +Y F + E+GY SA+A+V I+ +I+LI F +R Sbjct: 247 MLVYDIYDTAF---------KRYEYGYASAMAYVLFAIILVITLIQFRGQKR 289 >Q2BFH7.1 Q2BFH7_9BACI Sugar ABC transporter permease Length = 291 Score = 177 bits (449), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 100/284 (35%), Positives = 164/284 (57%), Gaps = 14/284 (4%) Query: 14 LLPSALLTLVLGIYPIAWAFRYMFYNYKGYGTARFIGLDNFSRILRDTEFWNSVVNTFVY 73 L P+ L+ LV I PI +F Y + G+ +F+GLDNF ++ +D+ FWNSV N Sbjct: 15 LAPALLIYLVFAILPILQSFYYSLMEWNGFSAMKFVGLDNFVQLFQDSLFWNSVKNNIYV 74 Query: 74 AGGKXXXXXXXXXXXXXXXNRKIRGRQLLRAVFFMPTVISTAVMAVVFFTIFNSYNGILN 133 NRKI+G ++ R + F+P V+ST V+++ + IFNS G++N Sbjct: 75 VLASVFGQVPIALFIALLLNRKIKGLKIFRTIGFLPVVLSTVVISLTWSLIFNSEQGLIN 134 Query: 134 QFLIKFNISSSGIDWLGP-KHAMLTVILVAVWGAVGNYMLLFLAGLQNIPEDVYESSSLD 192 + L + +WLG K +M+ V++V +W VG Y+++FLA LQN+P++V E++ +D Sbjct: 135 ELLRAVGLDFLAQNWLGDTKFSMIAVLVVVIWQFVGLYLIIFLAALQNVPQEVLEAAKID 194 Query: 193 GANKIQQFRFITLPMLGPVLQMVIMLAIINALKGYESIMVLTEGGPAGKTEVMFLYLYKL 252 GA++ IT+PM+ + + ++LAI +LK ++ I V+T GGPA T+VM LY++ Sbjct: 195 GASEWTTTWKITIPMIWDTIIVAVILAISGSLKTFDLIYVMTHGGPAHSTDVMALYMFNE 254 Query: 253 FFPVGGSAASTQVQEFGYGSAVAFVSAVIVGMISLIYFYASRRM 296 F +++Q +GYGSAV +V++ SLI Y ++ Sbjct: 255 TF--------SKLQ-YGYGSAV----SVVIFFFSLILIYTVTKL 285 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.142 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,573,874,188 Number of extensions: 59112107 Number of successful extensions: 230962 Number of sequences better than 1.0e-05: 5897 Number of HSP's gapped: 220197 Number of HSP's successfully gapped: 5904 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)