BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0010.p (334 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZAX6.1 C0ZAX6_BREBN Putative uncharacterized protein 253 3e-65 C1T972.1 C1T972_9FIRM Bacterial capsule synthesis protein PGA_cap 224 8e-57 A4J6T5.1 A4J6T5_DESRM Poly-gamma-glutamate synthesis protein (Ca... 215 6e-54 B0K8S9.1 B0K8S9_THEP3 Putative enzyme of poly-gamma-glutamate bi... 213 3e-53 B0K2U5.1 B0K2U5_THEPX Putative enzyme of poly-gamma-glutamate bi... 213 3e-53 >C0ZAX6.1 C0ZAX6_BREBN Putative uncharacterized protein Length = 439 Score = 253 bits (645), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 141/324 (43%), Positives = 187/324 (57%), Gaps = 17/324 (5%) Query: 18 SFDALFAKVAPYLKQADLTIGNLETTFAG-NGPNSRRTLRSSGPIFKCPDELAPALKKAG 76 SF +F VAP L ADLTIGNLETTF+G P + R+ P F CPDELA LK +G Sbjct: 116 SFTDMFHPVAPILSAADLTIGNLETTFSGKTQPYQIGSARTGYPRFNCPDELARDLKTSG 175 Query: 77 FDVLVTANNHCMDYGTPGLIRTLQVLDRNGISHTGTSRSLEESKQVLIKNVKGIKIGILS 136 FDVL T NNHC+D G GL RTL VLDR + HTGT RS +E+ LIK V GI+I +L+ Sbjct: 176 FDVLTTVNNHCLDGGVTGLCRTLDVLDRYKLVHTGTYRSPDEANTYLIKEVNGIRIAMLA 235 Query: 137 YTAGTNRIPVPANXXXXPWLVNRIETGKISREIRXXXXXXXXXXXYLHFGNEYHYTPNKR 196 YT GTN+ +PA+ PW+V + I ++R LHFG E+ YTP R Sbjct: 236 YTYGTNKQVIPAH---TPWIVRLLHLDTILSDLRKVRPLVDVVIISLHFGIEFRYTPTTR 292 Query: 197 QKQLVNLFFKHGANIILGSHPHVLQP------MESRGKKK--FVIYSLGNFVSTKLMNIP 248 Q+QLV +GA++ILG+HPHVLQP M G K+ V YSLGNF S K+++ Sbjct: 293 QRQLVQSLLDNGADVILGAHPHVLQPVVTPSIMTPHGTKRQTLVAYSLGNFTSEKMLSFD 352 Query: 249 YTQSGIILNLTIQKDQKGNTNITNVNYIPTGVDRRMASGSTMTEVVPIRDALKTGVPDRT 308 +Q G IL T++KD++ ++ ++ IPT R + G V+P+R LK+ PD Sbjct: 353 QSQCGAILRFTVEKDERNQISLEQISVIPTFSQRYVNQGRICFRVIPMRSYLKS--PDLY 410 Query: 309 GR---RNHLNRMLNHTESILKREK 329 R R L + + I+ R + Sbjct: 411 VRPLLRKKLQLLWSRAIRIIGRSR 434 >C1T972.1 C1T972_9FIRM Bacterial capsule synthesis protein PGA_cap Length = 397 Score = 224 bits (571), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 128/287 (44%), Positives = 171/287 (59%), Gaps = 13/287 (4%) Query: 16 THSFDALFAKVAPYLKQADLTIGNLETTFAGNGPNSRRTLRSSGPIFKCPDELAPALKKA 75 ++F +FA V YL+ +DLTIGNLET AG P S P+F CP +LA LKK Sbjct: 73 AYNFQPVFAPVEEYLQDSDLTIGNLETRLAG--PEKGY---SGYPLFNCPADLAVDLKKL 127 Query: 76 GFDVLVTANNHCMDYGTPGLIRTLQVLDRNGISHTGTSRSLEESKQVLIKNVKGIKIGIL 135 G D++ ANNHC+D G GLI T+ L++ G+ + G +RS EE +LIK++KGIK+G L Sbjct: 128 GIDIITLANNHCLDMGWNGLITTMNNLEQAGMQYIGNARSPEERDNLLIKDIKGIKLGFL 187 Query: 136 SYTAGTNRIPVPANXXXXPWLVNRIETGKISREIRXXXXX-XXXXXXYLHFGNEYHYTPN 194 +YT TN +PVP VN I I ++I YLHFG EY P Sbjct: 188 NYTETTNGLPVPEGKDFG---VNLINLDIIKKDIASLISNRADIVIVYLHFGTEYKRYPT 244 Query: 195 KRQKQLVNLFFKHGANIILGSHPHVLQPMESRGKKKFVIYSLGNFVSTKLMNIPYTQSGI 254 + QK+L F GA+IILG H HVLQPME G +KF+IYSLGNF+S + Y+ SGI Sbjct: 245 ENQKKLARQVFDAGADIILGDHVHVLQPMEREG-EKFIIYSLGNFISNQRWQ--YSDSGI 301 Query: 255 ILNLTIQKDQKGN-TNITNVNYIPTGVDRRMASGSTMTEVVPIRDAL 300 ILNLT +K+ + N T++ N++YIP VD A+G V+ + A+ Sbjct: 302 ILNLTFEKNLEQNKTHLKNIDYIPVWVDTYPAAGMLRYRVLAVEKAI 348 >A4J6T5.1 A4J6T5_DESRM Poly-gamma-glutamate synthesis protein (Capsule biosynthesis protein) Length = 396 Score = 215 bits (547), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 118/303 (38%), Positives = 175/303 (57%), Gaps = 18/303 (5%) Query: 6 IISEARRSDGTHSFDALFAKVAPYLKQADLTIGNLETTFAGNGPNSRRTLRSSGPIFKCP 65 II A+ G + F +F++V +L A+LT+ NLET AG + S P+F CP Sbjct: 58 IIHAAQLPGGNYDFKPIFSEVRSFLSTAELTLVNLETRLAG-----KEQGYSGYPVFNCP 112 Query: 66 DELAPALKKAGFDVLVTANNHCMDYGTPGLIRTLQVLDRNGISHTGTSRSLEESKQVLIK 125 +ELA +K+ G DV+ TANNH +D G G+++TL L+ G+ GT RS E++++V Sbjct: 113 EELAQNMKELGIDVVTTANNHSLDRGWTGIVKTLDHLEAAGLQSLGTYRSAEDAQRVFCT 172 Query: 126 NVKGIKIGILSYTAGTNRIPVPANXXXXPWLVNRIETGKISREI-RXXXXXXXXXXXYLH 184 +KGIKIGIL+YT TN IP+P + V+ I++GKI ++I + Y+H Sbjct: 173 EIKGIKIGILNYTESTNGIPLPKG---KDYAVDMIQSGKIYQDIEKLKAAKTDVIIAYIH 229 Query: 185 FGNEYHYTPNKRQKQLVNLFFKHGANIILGSHPHVLQPMESR------GKKKFVIYSLGN 238 FG EY PN+ Q+ LV FK G I+ G H HV+QPM+ + KK FV YSLGN Sbjct: 230 FGTEYQRHPNESQRTLVENLFKQGVTIVFGDHVHVIQPMDLKVSSTNNEKKCFVAYSLGN 289 Query: 239 FVSTKLMNIPYTQSGIILNLTIQKDQKGNTNITNVNYIPTGVDRRMASGSTMTEVVPIRD 298 F+S + + Y+ G+I N+ + K + ITNV+Y+P VD +G V+P++ Sbjct: 290 FISNQ--SWRYSNCGLITNVKLTK-EANRVTITNVDYVPVWVDTYFKNGQKKYRVLPVQK 346 Query: 299 ALK 301 A++ Sbjct: 347 AIE 349 >B0K8S9.1 B0K8S9_THEP3 Putative enzyme of poly-gamma-glutamate biosynthesis (Capsule formation)-like protein Length = 392 Score = 213 bits (541), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 123/296 (41%), Positives = 179/296 (60%), Gaps = 17/296 (5%) Query: 10 ARRSDGTHSFDALFAKVAPYLKQADLTIGNLETTFAGNGPNSRRTLRSSGPIFKCPDELA 69 A + DG++ F F +V Y+K +D+ I NLETT + P T P F+ P+E+ Sbjct: 68 AIKPDGSYDFTPHFEEVREYIKDSDIAICNLETTIST--PVKGYT---GYPRFRSPEEII 122 Query: 70 PALKKAGFDVLVTANNHCMDYGTPGLIRTLQVLDRNGISHTGTSRSLEESKQVLIKNVKG 129 PA+K GF+V++TANNH +D G++ TL LD+ G+ H GT R+ EESK++LI + KG Sbjct: 123 PAIKYTGFNVVITANNHSLDNLEFGVVNTLNALDKYGLFHAGTYRNEEESKKILIIDKKG 182 Query: 130 IKIGILSYTAGTNRIPVPANXXXXPWLVNRIETGKISREI-RXXXXXXXXXXXYLHFGNE 188 IK+ IL+YT GTN + V + ++VN IE KI ++I R YLH+G E Sbjct: 183 IKVAILAYTFGTNGMEVHIDRGKLKYMVNYIEEEKILKDINRAKEEGADIVILYLHWGME 242 Query: 189 YHYTPNKRQKQLVNLFFKHGANIILGSHPHVLQPMESR------GKKK--FVIYSLGNFV 240 Y T ++ +K L + F+ GA+IILG+HPHV+QPME + G++K FV YSLGNF+ Sbjct: 243 YARTVDEERKTLTDKLFRAGADIILGTHPHVIQPMEKKKITTDDGEEKEVFVAYSLGNFI 302 Query: 241 STKLMNIPYTQSGIILNLTIQKDQKGNTNITNVNYIPTGVDRRMASGSTMTEVVPI 296 S + Y SG+I+N+TI K + T+IT V+Y+PT V G T ++P+ Sbjct: 303 SNQRDR--YQDSGVIVNITITK-KNDKTSITEVSYVPTWVRVFKKEGKTQYRILPV 355 >B0K2U5.1 B0K2U5_THEPX Putative enzyme of poly-gamma-glutamate biosynthesis (Capsule formation)-like protein Length = 392 Score = 213 bits (541), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 123/296 (41%), Positives = 179/296 (60%), Gaps = 17/296 (5%) Query: 10 ARRSDGTHSFDALFAKVAPYLKQADLTIGNLETTFAGNGPNSRRTLRSSGPIFKCPDELA 69 A + DG++ F F +V Y+K +D+ I NLETT + P T P F+ P+E+ Sbjct: 68 AIKPDGSYDFTPHFEEVREYIKDSDIAICNLETTIST--PVKGYT---GYPRFRSPEEII 122 Query: 70 PALKKAGFDVLVTANNHCMDYGTPGLIRTLQVLDRNGISHTGTSRSLEESKQVLIKNVKG 129 PA+K GF+V++TANNH +D G++ TL LD+ G+ H GT R+ EESK++LI + KG Sbjct: 123 PAIKYTGFNVVITANNHSLDNLEFGVVNTLNALDKYGLFHAGTYRNEEESKKILIIDKKG 182 Query: 130 IKIGILSYTAGTNRIPVPANXXXXPWLVNRIETGKISREI-RXXXXXXXXXXXYLHFGNE 188 IK+ IL+YT GTN + V + ++VN IE KI ++I R YLH+G E Sbjct: 183 IKVAILAYTFGTNGMEVHIDRGKLKYMVNYIEEEKILKDINRAKEEGADIVILYLHWGME 242 Query: 189 YHYTPNKRQKQLVNLFFKHGANIILGSHPHVLQPMESR------GKKK--FVIYSLGNFV 240 Y T ++ +K L + F+ GA+IILG+HPHV+QPME + G++K FV YSLGNF+ Sbjct: 243 YARTVDEERKTLTDKLFRAGADIILGTHPHVIQPMEKKKITTDEGEEKEVFVAYSLGNFI 302 Query: 241 STKLMNIPYTQSGIILNLTIQKDQKGNTNITNVNYIPTGVDRRMASGSTMTEVVPI 296 S + Y SG+I+N+TI K + T+IT V+Y+PT V G T ++P+ Sbjct: 303 SNQRDR--YQDSGVIVNITITK-KNDKTSITEVSYVPTWVRVFKKEGKTQYRILPV 355 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,826,219,622 Number of extensions: 72523010 Number of successful extensions: 158393 Number of sequences better than 1.0e-05: 552 Number of HSP's gapped: 157188 Number of HSP's successfully gapped: 568 Length of query: 334 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 198 Effective length of database: 1,779,438,163 Effective search space: 352328756274 Effective search space used: 352328756274 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)