BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0007.p (490 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEQ6.1 B3KEQ6_9BACI Extracellular solute-binding protein family 1 796 0.0 B1D7Q4.1 B1D7Q4_9BACL Extracellular solute-binding protein family 1 497 e-139 B3K5P2.1 B3K5P2_9BACI Extracellular solute-binding protein family 1 485 e-135 A9KI47.1 A9KI47_CLOPH Extracellular solute-binding protein family 1 294 1e-77 Q98EP6.1 Q98EP6_RHILO Sugar ABC transporter, sugar-binding protein 98 2e-18 >B3KEQ6.1 B3KEQ6_9BACI Extracellular solute-binding protein family 1 Length = 510 Score = 796 bits (2056), Expect = 0.0, Method: Compositional matrix adjust. Identities = 390/488 (79%), Positives = 416/488 (85%), Gaps = 7/488 (1%) Query: 3 DEFTIQMLIKGVERMQKKWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 62 +E TIQ LIKGVE M+KKW Sbjct: 30 NELTIQTLIKGVECMRKKWLSLGLSLILAAGIAGCGGNDNSSNGGTTNGKSDGGTTAP-- 87 Query: 63 XXXXXNTAKSGPVQLKYWTDDRHDQEYIKELINKFNETNSDNIQVELTVMSENYTQSVDI 122 +T PVQLKYWTDDRHDQEYIKELINKFNETN DNIQVELTVMSENY QSVDI Sbjct: 88 -----STETGQPVQLKYWTDDRHDQEYIKELINKFNETNGDNIQVELTVMSENYAQSVDI 142 Query: 123 AFSSNQAPDVLRLKSANTSEFVKKGYLAPVDSYLTDDMKTKFSSLMLDNVNRFDGKLYSL 182 AFSSNQAPDVLRLKSANTS FVKKGYLAP+D YLTDD+K KF SL++D+VNRFDGK+YSL Sbjct: 143 AFSSNQAPDVLRLKSANTSGFVKKGYLAPIDEYLTDDLKAKFGSLLIDDVNRFDGKVYSL 202 Query: 183 PNTGLTMRLVYNKDIFAKAGIQNPPVSLQEMVDDAKKITEAGKSEGIYGFALNFKNPKQA 242 NTGLTMRLVYNKD+FAKAGI+NPP SLQEMV+DAKKITE GK+EGIYGFALNFKNPKQA Sbjct: 203 ANTGLTMRLVYNKDLFAKAGIENPPKSLQEMVEDAKKITEVGKAEGIYGFALNFKNPKQA 262 Query: 243 FDRSIREILSLSGYQGLGFDLKTGQFDFAPYSQVIEYFKQMYKDGSVLPGAETLDIDPLR 302 FDRS+REILSLSGYQGLGFDLKTGQFDF PYSQVIEYFKQM+KDGS+LPGAE LDIDPLR Sbjct: 263 FDRSVREILSLSGYQGLGFDLKTGQFDFEPYSQVIEYFKQMHKDGSILPGAEALDIDPLR 322 Query: 303 AQFAAGKIGMYLSFSTEPGVYKDQFPTEINWAGALAPTLDGQIKGTSEIVSAGTWLGISA 362 AQFAAGKIGMYLSFSTEPGVY DQFPTEINW GALAPTLDG+IKGTSEIVSAGTWLGISA Sbjct: 323 AQFAAGKIGMYLSFSTEPGVYMDQFPTEINWGGALAPTLDGEIKGTSEIVSAGTWLGISA 382 Query: 363 KSANQDAAWKFMQYMYGDEVLKTYHEKGFGIAVVPSIVEQAKNPDIKGMEGFLVGEHDSL 422 +SAN++AAWKFMQYMYGDEVLKTYHEKGFGIA+VPSIVEQAK+P+I GMEGFLVGEHDSL Sbjct: 383 QSANKEAAWKFMQYMYGDEVLKTYHEKGFGIAIVPSIVEQAKDPEINGMEGFLVGEHDSL 442 Query: 423 WPATPSVTPEGSNYADAFFKYILTGGDAKAITEDLNTRYNAALSKAVEKGEVKVTPDPAF 482 WPA+P+VTPEGS YADAFFKYILT D KAITEDLNTRYNAALSKAVEKGEV VTP+PAF Sbjct: 443 WPASPNVTPEGSTYADAFFKYILTDEDLKAITEDLNTRYNAALSKAVEKGEVTVTPNPAF 502 Query: 483 DPTKPQGE 490 DPTKPQGE Sbjct: 503 DPTKPQGE 510 >B1D7Q4.1 B1D7Q4_9BACL Extracellular solute-binding protein family 1 Length = 475 Score = 497 bits (1280), Expect = e-139, Method: Compositional matrix adjust. Identities = 230/421 (54%), Positives = 310/421 (73%) Query: 69 TAKSGPVQLKYWTDDRHDQEYIKELINKFNETNSDNIQVELTVMSENYTQSVDIAFSSNQ 128 T K+ V+L YWT+DRHD YI+E++++FNETN+DNI+VEL +SENY QSVDIAFSS Q Sbjct: 51 TTKAEKVKLNYWTEDRHDLTYIQEVVDRFNETNTDNIEVELKGLSENYNQSVDIAFSSKQ 110 Query: 129 APDVLRLKSANTSEFVKKGYLAPVDSYLTDDMKTKFSSLMLDNVNRFDGKLYSLPNTGLT 188 APD++R +N FVKKGYL P+D YL D+MKTKF ++D N +G++YSLPN G + Sbjct: 111 APDIIRTTPSNFIPFVKKGYLEPLDGYLNDEMKTKFQPALVDKSNMMNGQIYSLPNYGYS 170 Query: 189 MRLVYNKDIFAKAGIQNPPVSLQEMVDDAKKITEAGKSEGIYGFALNFKNPKQAFDRSIR 248 RL+YN D+F KAGI +PP +L +MV+ AKK+TEAGK G YGFA+NFK +F+++ R Sbjct: 171 SRLIYNVDLFEKAGITSPPTTLNDMVEVAKKLTEAGKDIGAYGFAINFKGADNSFEKAPR 230 Query: 249 EILSLSGYQGLGFDLKTGQFDFAPYSQVIEYFKQMYKDGSVLPGAETLDIDPLRAQFAAG 308 I LSG G G+D KTG++DF+ ++ +++ FKQM DGS+LPG E LDIDPLRAQFA G Sbjct: 231 SIAELSGLGGYGYDFKTGKYDFSGFTDIMKAFKQMKDDGSMLPGVEALDIDPLRAQFAEG 290 Query: 309 KIGMYLSFSTEPGVYKDQFPTEINWAGALAPTLDGQIKGTSEIVSAGTWLGISAKSANQD 368 KIGMYLS S EP VY+ QFPT++NW+ AL PT++G I G + G WL IS+++ N+ Sbjct: 291 KIGMYLSISAEPYVYQTQFPTKVNWSAALPPTINGNIAGVAGYWKTGPWLSISSQTKNKK 350 Query: 369 AAWKFMQYMYGDEVLKTYHEKGFGIAVVPSIVEQAKNPDIKGMEGFLVGEHDSLWPATPS 428 AW F++YMY DEVLKTY+E G+G++++P IV Q+KNP++ G+ GFL ++D LWP +PS Sbjct: 351 EAWTFLEYMYSDEVLKTYYEDGYGMSLLPEIVAQSKNPEMPGVAGFLPTQYDGLWPISPS 410 Query: 429 VTPEGSNYADAFFKYILTGGDAKAITEDLNTRYNAALSKAVEKGEVKVTPDPAFDPTKPQ 488 VT EG+++++ F KY+++GGD + D NTRYNAAL KA G+V P FD Q Sbjct: 411 VTVEGTSFSNLFIKYVISGGDLDKMISDANTRYNAALDKARASGDVTTEAMPDFDAKSMQ 470 Query: 489 G 489 G Sbjct: 471 G 471 >B3K5P2.1 B3K5P2_9BACI Extracellular solute-binding protein family 1 Length = 456 Score = 485 bits (1248), Expect = e-135, Method: Compositional matrix adjust. Identities = 222/416 (53%), Positives = 305/416 (73%), Gaps = 3/416 (0%) Query: 75 VQLKYWTDDRHDQEYIKELINKFNETNSDNIQVELTVMSENYTQSVDIAFSSNQAPDVLR 134 ++ YWT DRHD E++KE I+ FN+TN DNI+VEL + +++ Q++D++F +++ PD++R Sbjct: 41 TKITYWTGDRHDSEFVKETIDTFNKTNKDNIEVELVIKGDDFDQALDMSFQTSEPPDIIR 100 Query: 135 LKSANTSEFVKKGYLAPVDSYLTDDMKTKFSSLMLDNVNRFDGKLYSLPNTGLTMRLVYN 194 +K F KKG+LAP+D +LTD+MK KF +++++N F+GK YSLPN G TMRL+YN Sbjct: 101 VKENTIQTFYKKGFLAPIDEFLTDEMKEKFP--LMEDLNSFEGKRYSLPNYGTTMRLIYN 158 Query: 195 KDIFAKAGIQNPPVSLQEMVDDAKKITEAGKSEGIYGFALNFKNPKQAFDRSIREILSLS 254 K++FAKAGI+ PP +L E+V+ AKKITEAGK+ G YGFA NFKNP A RS R I +S Sbjct: 159 KELFAKAGIEQPPTTLDELVETAKKITEAGKASGAYGFAQNFKNPSSALGRSARVIAEVS 218 Query: 255 GYQGLGFDLKTGQFDFAPYSQVIEYFKQMYKDGSVLPGAETLDIDPLRAQFAAGKIGMYL 314 G+ G G+D KT ++DF+ + ++E FKQ+ DGS+LPG E+LDIDPLRAQFA GKIGMYL Sbjct: 219 GFGGFGYDFKTARYDFSGFKPIMEAFKQIKDDGSMLPGVESLDIDPLRAQFAEGKIGMYL 278 Query: 315 SFSTEPGVYKDQFPTEINWAGALAPTLDGQIKGTSEIVSAGTWLGISAKSANQDAAWKFM 374 SFS E GVY+ QFP +I WA A P++DGQ G S + G WL IS++S +++AAWKF+ Sbjct: 279 SFSAEAGVYQSQFPAKIEWAAAPVPSIDGQFNGASGFL-GGQWLAISSESEHKEAAWKFL 337 Query: 375 QYMYGDEVLKTYHEKGFGIAVVPSIVEQAKNPDIKGMEGFLVGEHDSLWPATPSVTPEGS 434 YMY + +L+ Y EKGFGI++VPS+ A P++KG++GFL ++D +WP P+V +G Sbjct: 338 TYMYDESILQQYQEKGFGISMVPSVSAVAAKPEVKGIDGFLPNQYDGVWPVYPTVPVQGM 397 Query: 435 NYADAFFKYILTGGDAKAITEDLNTRYNAALSKAVEKGEVKVTPDPAFDPTKPQGE 490 DAFFKYIL+GGD +I +DLNTRYNAAL A+ GEVK P F+P G+ Sbjct: 398 KSDDAFFKYILSGGDLDSIIDDLNTRYNAALDAAIASGEVKAEPMADFNPLDLAGQ 453 >A9KI47.1 A9KI47_CLOPH Extracellular solute-binding protein family 1 Length = 465 Score = 294 bits (753), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 161/417 (38%), Positives = 232/417 (55%), Gaps = 10/417 (2%) Query: 80 WTDDRHDQEYIKELINKFNETNSDNIQVELTVMSENYTQSVDIAFSSNQAPDVLRLKSAN 139 WT DRHD E+ E I ++N +NSDNI++ ++ S+NY Q+VD+AF SN APD+L S Sbjct: 50 WTKDRHDAEFQLEKIEEYNASNSDNIEIRYSIFSDNYLQAVDLAFQSNSAPDILVFTSQV 109 Query: 140 TSEFVKKGYLAPVDSYLTDDMKTKFSSLMLDNVNRFDGKLYSLPNTGLTMRLVYNKDIFA 199 + +V A + ++ + K FSS M++ +N DGK Y +P RL YNKDIF Sbjct: 110 FNNYVVSDQFADLTPFMDKEFKETFSSSMIEGLNVIDGKCYYIPTAATICRLFYNKDIFE 169 Query: 200 KAGIQNPPVSLQEMVDDAKKITEAGKSEGIYGFALNFKNPKQAFDRSIREILSLSGYQGL 259 + G+ PP +L EMV+ A+KIT+ EGIYGF+ N K P A +RS+ + + Sbjct: 170 RVGVLEPPRTLDEMVEIAQKITKELSGEGIYGFSANMKYPNSALNRSLMPMAQMGLGIRS 229 Query: 260 GFDLKTGQFDFAPYSQVIEYFKQMYKDGSVLPGAETLDIDPLRAQFAAGKIGMYLSFS-T 318 GFD K G +DF+ Y ++E ++ + P +++LDIDPLR FAAGKIGMY+S++ + Sbjct: 230 GFDFKKGVYDFSGYQTILEEWRTLLSPECAYPNSDSLDIDPLRKLFAAGKIGMYMSYAHS 289 Query: 319 EPGVYKDQFPTEINWAGALAPTLDGQIKGTSEIVSAGTWLGISAKSANQDAAWKFMQYMY 378 E GVY++QFP E W P + G I G +L +AKS N DAAWK ++ Sbjct: 290 ELGVYENQFPMEQEWRCTEIPVVGGIILGAQNYSLNNGYL-FNAKSKNLDAAWKAYVSVF 348 Query: 379 GD-EVLKTYHEKGFGIAVVPSIVEQAK-NPDIKGMEGFLVGEHDSLWPATP------SVT 430 D + L YH +G GI+ VP ++E+A N LVGE+D +WP P ++ Sbjct: 349 ADIDNLAEYHSQGLGISTVPKVLERATLNQSYIDNPALLVGENDKIWPKVPHEANVNAIV 408 Query: 431 PEGSNYADAFFKYILTGGDAKAITEDLNTRYNAALSKAVEKGEVKVTPDPAFDPTKP 487 +G + + F + I D K DL RYN A + + G +V FDP P Sbjct: 409 VDGLDMYNTFAELIYGTMDIKEGLSDLTKRYNKAYQEGINSGIGEVYKIDDFDPLNP 465 >Q98EP6.1 Q98EP6_RHILO Sugar ABC transporter, sugar-binding protein Length = 408 Score = 97.8 bits (242), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 96/338 (28%), Positives = 148/338 (43%), Gaps = 36/338 (10%) Query: 142 EFVKKGYLAPVDSYLTDDMKTKFSSLMLDNVNRFDGKLYSLPNTGLTMRLVYNKDIFAKA 201 +FV++ P+DSY+ D K +F L + + DGK Y LP L YNK++F KA Sbjct: 95 DFVQQDVAEPLDSYIKPDFKGRFIDTFL-SPSIMDGKTYGLPIAASARALYYNKELFEKA 153 Query: 202 GIQNPPVSLQEMVDDAKKITEAGKSEGIYGFALNFKNPKQ------AFDRSIREILSLSG 255 GI PP + E+ DDA+KI K+ G +GF L K + A EIL+ G Sbjct: 154 GIAKPPATWTELQDDARKI----KALGSFGFGLQGKEIETDVYYYYAMWSQGTEILNKDG 209 Query: 256 YQGLGFDLKTGQFDFAPYSQVIEYFKQMYKDGSVLPGAETLDIDPLRAQFAAGKIGMYLS 315 GLG G + A + +K M +G PG + + + ++ F GK+GM ++ Sbjct: 210 TSGLG---TPGALEAA------KLYKSMIDEGLTEPGVTSNNREDVQNLFKQGKVGMMIT 260 Query: 316 FSTEPGVYKDQFPTEINWAGALAPTLDGQIKGTSEIVSAGTWLGISAKSANQDAAWKFMQ 375 KD+ P ++ + A P +GT + + + + S N+D AWK + Sbjct: 261 APFLSNQIKDEAP-KLKYGVAAIPAGPTGARGTYGVTDS---IIMFKNSKNKDEAWKLLD 316 Query: 376 YMYGDEVLKTYHEKGFGIAVVPSIVEQAK------NPDIKGMEGFLVGEHDSLWPATPSV 429 +++ E + + G +P E+AK N D+ L P P Sbjct: 317 FLFTTEQRAKFTQ---GEGFLPVNKEEAKMDYYVNNADLAAFTALL--PDARFAPVIPGW 371 Query: 430 TPEGSNYADAFFKYILTGGDAKAITEDLNTRYNAALSK 467 +DA K I GGD +A +D + NA L K Sbjct: 372 EEIAQITSDAMQK-IYLGGDPEAGLKDAAAKANAVLKK 408 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,718,742,908 Number of extensions: 113952961 Number of successful extensions: 256444 Number of sequences better than 1.0e-05: 1180 Number of HSP's gapped: 256530 Number of HSP's successfully gapped: 1180 Length of query: 490 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 350 Effective length of database: 1,741,754,103 Effective search space: 609613936050 Effective search space used: 609613936050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)