BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0385.0005.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEQ8.1 B3KEQ8_9BACI Binding-protein-dependent transport system... 529 e-148 B1D7Q2.1 B1D7Q2_9BACL Binding-protein-dependent transport system... 382 e-104 B3K5P0.1 B3K5P0_9BACI Binding-protein-dependent transport system... 356 1e-96 Q2CEJ0.1 Q2CEJ0_9RHOB Sugar permease 160 2e-37 Q8R7J2.1 Q8R7J2_THETN Sugar permeases 157 1e-36 >B3KEQ8.1 B3KEQ8_9BACI Binding-protein-dependent transport systems inner membrane component Length = 300 Score = 529 bits (1362), Expect = e-148, Method: Compositional matrix adjust. Identities = 263/300 (87%), Positives = 274/300 (91%), Gaps = 1/300 (0%) Query: 1 MNEQVLSQQPPVREKMAARP-SGNAGRILGRTVMWXXXXXXXXXXXXXXXMTVSGSLKTG 59 MNEQVL QQP R K+AARP SG+ GRI+GRTVMW MT+SGSLKTG Sbjct: 1 MNEQVLPQQPDARGKIAARPPSGHTGRIVGRTVMWLFLLITAILTLFPLLMTLSGSLKTG 60 Query: 60 AEMMTGGSLLPSKLQFANYAEAWKQANFARYTWNSAFVSIMVTVGTLLVASMAAYVVDRR 119 AEMMTGGSLLP+KLQF NYAEAWKQANFARYTWNSAFVSIMVT GTLLVA+MAAYVVDRR Sbjct: 61 AEMMTGGSLLPAKLQFVNYAEAWKQANFARYTWNSAFVSIMVTAGTLLVAAMAAYVVDRR 120 Query: 120 DFPGKSLYVTVQASMMFISVGAIVLRPQFDLMVALHLNTTLWGVILILVSAHSSTFFMLQ 179 FPGKSLYVT QASMMFISVGAIVLRPQFDLMVAL+LNTTLWGVILIL+SAHSSTFFML Sbjct: 121 HFPGKSLYVTTQASMMFISVGAIVLRPQFDLMVALNLNTTLWGVILILISAHSSTFFMLL 180 Query: 180 GFFKAIPRELDEAAMVDGSGFIRTFFRIILPLLTPGLGVAGLFAFRHAWNEYILPLVFTM 239 GFFKAIPRELDEAAMVDGSGF+RTFFRIILPLLTPGLGVAGLFAFRHAWNEYILPLVFTM Sbjct: 181 GFFKAIPRELDEAAMVDGSGFVRTFFRIILPLLTPGLGVAGLFAFRHAWNEYILPLVFTM 240 Query: 240 TNPQLQTLTVGLANLRYGSSAAMQIHLMMAGACLSILPMLLAYILANKTFIQVTAGSVKG 299 TNPQLQTLTVGLANLRYGSSAAMQIHLMMAGACLSILPMLLAYI+ANK+FIQVTAGSVKG Sbjct: 241 TNPQLQTLTVGLANLRYGSSAAMQIHLMMAGACLSILPMLLAYIVANKSFIQVTAGSVKG 300 >B1D7Q2.1 B1D7Q2_9BACL Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 382 bits (981), Expect = e-104, Method: Compositional matrix adjust. Identities = 180/299 (60%), Positives = 232/299 (77%), Gaps = 5/299 (1%) Query: 1 MNEQVLSQQPPVREKMAARPSGNAGRILGRTVMWXXXXXXXXXXXXXXXMTVSGSLKTGA 60 MN + P+ K+ R A RI ++W +T+ GS KT A Sbjct: 1 MNTAPATNTAPI-TKIGRR----AKRIFSGFILWVFLGIVAVFTVFPVLITIFGSFKTNA 55 Query: 61 EMMTGGSLLPSKLQFANYAEAWKQANFARYTWNSAFVSIMVTVGTLLVASMAAYVVDRRD 120 E+ G ++LPS F+NY +AWKQA+FA++TWNS F+S + T+GTLL+ASMAAYV+DRR+ Sbjct: 56 ELTAGATILPSVWHFSNYYDAWKQADFAKFTWNSLFISTITTIGTLLLASMAAYVIDRRN 115 Query: 121 FPGKSLYVTVQASMMFISVGAIVLRPQFDLMVALHLNTTLWGVILILVSAHSSTFFMLQG 180 F GKS+YV++QA+ MFIS+GA+V+RPQF+LMV LHLN++LWG+I IL+SAH++TFF+L G Sbjct: 116 FFGKSIYVSLQAATMFISIGAVVIRPQFELMVKLHLNSSLWGIIFILMSAHATTFFILIG 175 Query: 181 FFKAIPRELDEAAMVDGSGFIRTFFRIILPLLTPGLGVAGLFAFRHAWNEYILPLVFTMT 240 FFK IPR+LDEAA +DG F ++RI+LPLL PGLGVA LFAFR+AWNEYILPLVFTMT Sbjct: 176 FFKGIPRDLDEAAHIDGCNFFSVYWRIVLPLLKPGLGVAALFAFRNAWNEYILPLVFTMT 235 Query: 241 NPQLQTLTVGLANLRYGSSAAMQIHLMMAGACLSILPMLLAYILANKTFIQVTAGSVKG 299 NP+LQTLTVGLA+LRYG++AA Q HL+MAGACLSILP+L+ Y+ ANK+F+QVT GSVKG Sbjct: 236 NPKLQTLTVGLADLRYGTTAATQTHLIMAGACLSILPILIVYVFANKSFMQVTGGSVKG 294 >B3K5P0.1 B3K5P0_9BACI Binding-protein-dependent transport systems inner membrane component Length = 275 Score = 356 bits (914), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 172/274 (62%), Positives = 211/274 (77%) Query: 26 RILGRTVMWXXXXXXXXXXXXXXXMTVSGSLKTGAEMMTGGSLLPSKLQFANYAEAWKQA 85 RI T++W +T+ GS KT AE+ G + LP+ QF+NY EAW+QA Sbjct: 2 RIAWNTILWAFLIVVAFLTLFPVVITLLGSFKTNAELTAGATFLPADWQFSNYIEAWEQA 61 Query: 86 NFARYTWNSAFVSIMVTVGTLLVASMAAYVVDRRDFPGKSLYVTVQASMMFISVGAIVLR 145 NF+ YT NS FV+ T+GTLLVASMAAYVV+R DF GK LYV +QA MF+++GA+VLR Sbjct: 62 NFSTYTLNSLFVAGAATIGTLLVASMAAYVVNRMDFIGKKLYVGLQAFTMFVAIGAVVLR 121 Query: 146 PQFDLMVALHLNTTLWGVILILVSAHSSTFFMLQGFFKAIPRELDEAAMVDGSGFIRTFF 205 PQFDLM+ LHL+ +LWGVILIL+SAH+S FF+L F IPRELDEAA +DG RTF+ Sbjct: 122 PQFDLMIRLHLHNSLWGVILILISAHASAFFILLSFMNGIPRELDEAARIDGCSPGRTFW 181 Query: 206 RIILPLLTPGLGVAGLFAFRHAWNEYILPLVFTMTNPQLQTLTVGLANLRYGSSAAMQIH 265 RII+PLLTPGLGV LFAFR AWNEY+LP VFT++ P+LQTLTVGLANL+YG +AA Q H Sbjct: 182 RIIIPLLTPGLGVCALFAFRGAWNEYLLPFVFTLSKPELQTLTVGLANLKYGVAAASQTH 241 Query: 266 LMMAGACLSILPMLLAYILANKTFIQVTAGSVKG 299 MMAGACLSILP+L+AYI ANK+F+Q+TAGS+KG Sbjct: 242 FMMAGACLSILPILIAYIFANKSFMQMTAGSLKG 275 >Q2CEJ0.1 Q2CEJ0_9RHOB Sugar permease Length = 294 Score = 160 bits (404), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 85/249 (34%), Positives = 145/249 (58%), Gaps = 4/249 (1%) Query: 52 VSGSLKTGAEMMTGGSLLPSKLQFANYAEAWKQANFARYTWNSAFVSIMVTVGTLLVASM 111 +S S KT E+ L+P F NY AW++AN +Y +N+ FV++ + T + A++ Sbjct: 49 ISASFKTNGEIFADSGLIPDAPTFDNYTRAWREANIGQYFFNTLFVTVGSVIITTIAAAL 108 Query: 112 AAYVVDRRDFPGKSLYVTVQASMMFISVGAIVLRPQFDLMVALHLNTTLWGVILILVSAH 171 YV+ RR PGK+ + + +FI G ++ P F+L+ ++ L +LWGV+L Sbjct: 109 MGYVLGRRPIPGKTALMGLMVFTLFIPQGFTII-PVFELLTSVGLGQSLWGVMLATCGHS 167 Query: 172 SSTF-FMLQGFFKAIPRELDEAAMVDGSGFIRTFFRIILPLLTPGLGVAGLFAFRHAWNE 230 F F+ G+F +P+EL+EA+ +DG G ++TF+ ++LPL P + + AWN+ Sbjct: 168 IVIFTFLFAGYFGQMPKELEEASRMDGVGPLKTFWYVMLPLSKPIIVTVIVMQTLQAWND 227 Query: 231 YILPLVFTMTNPQLQTLTVGLANLRYGSSAAMQIHLMMAGACLSILPMLLAYILANKTFI 290 ++LPLV T+ NP L+TL+VG+ + R + M A + ++I+P++L ++ + FI Sbjct: 228 FLLPLVVTLANPDLRTLSVGVYSFR--GEHFIDWGGMTAASAITIIPIILLFLFLQRYFI 285 Query: 291 QVTAGSVKG 299 AG+VKG Sbjct: 286 DGLAGAVKG 294 >Q8R7J2.1 Q8R7J2_THETN Sugar permeases Length = 272 Score = 157 bits (396), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 93/250 (37%), Positives = 145/250 (58%), Gaps = 8/250 (3%) Query: 53 SGSLKTGAEMMTGGSLLPSKLQFANYAEAWKQANFARYTWNSAFVSIMVTVGTLLVASMA 112 S SLKT +E+ T SL+PS F NY +AW + NF+ Y NS +I + G ++V+++A Sbjct: 28 STSLKTRSEVYTSRSLIPSHWNFDNYVKAWTEGNFSIYFLNSVIYTIAIVAGVVIVSTLA 87 Query: 113 AYVVDRRDFPGKSLYVTVQASMMFISV-GAIVLRPQFDLMVALHLNTTLWGVILILV-SA 170 AY R DFP K+L+ M I + G+ + P + L+V L + T G IL ++ S Sbjct: 88 AYGFARLDFPLKNLFYYAFLVFMMIPIPGSFI--PLYVLLVKLGIQNTRIGYILPMINSG 145 Query: 171 HSSTFFMLQGFFKAIPRELDEAAMVDGSGFIRTFFRIILPLLTPGLGVAGLFAFRHAWNE 230 + F+L+GFF+ IP+EL+EAA++DG + +++I+ PL P + +F AWNE Sbjct: 146 LAVAIFILKGFFEEIPKELEEAAIMDGCNKFQIYYKIMFPLAMPAISTIIIFNTLSAWNE 205 Query: 231 YILPLVFTMTNPQLQTLTVGLANLRYGSSAAMQIHLMMAGACLSILPMLLAYILANKTFI 290 YIL V TNP L + GL + + Q L+MA ++ LP++L Y++ K I Sbjct: 206 YILAAV-NFTNPNLMPIQQGL--VVFQGQYFTQYELLMAANVITTLPIILVYLVLQKYII 262 Query: 291 Q-VTAGSVKG 299 + +TAG++KG Sbjct: 263 KGITAGAIKG 272 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,528,679,412 Number of extensions: 54691768 Number of successful extensions: 202021 Number of sequences better than 1.0e-05: 6099 Number of HSP's gapped: 195175 Number of HSP's successfully gapped: 6100 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)