BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0031.p (418 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3HA40.1 C3HA40_BACTU PKS acyltransferase domain 590 e-167 B9E2D2.1 B9E2D2_CLOK1 Putative uncharacterized protein 441 e-122 A5N8Z3.1 A5N8Z3_CLOK5 Predicted PKS acyltransferase domain 441 e-122 B8I5H7.1 B8I5H7_CLOCE Malonyl CoA-acyl carrier protein transacylase 419 e-115 B8I5C8.1 B8I5C8_CLOCE Malonyl CoA-acyl carrier protein transacylase 399 e-109 >C3HA40.1 C3HA40_BACTU PKS acyltransferase domain Length = 418 Score = 590 bits (1521), Expect = e-167, Method: Compositional matrix adjust. Identities = 277/419 (66%), Positives = 345/419 (82%), Gaps = 2/419 (0%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 MSK+ FLFSGQGSQY+GMGK+LY+NF+IAKETY EASEVLGFDLG+LCFEG+++ELS+TE Sbjct: 1 MSKLGFLFSGQGSQYIGMGKNLYNNFAIAKETYDEASEVLGFDLGKLCFEGNANELSQTE 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 NTQPAIL S+AQ+RVYM+EIGE+P Y AGHSLGEY+AL AG I+F+DAVKLVRARG++ Sbjct: 61 NTQPAILTTSMAQFRVYMEEIGEEPHYLAGHSLGEYTALVCAGVIDFSDAVKLVRARGMF 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERL 180 MK IR++++ ++EEE +S GR+IGISNYNG KD+V+SGEA+AV ++ Sbjct: 121 MKEIANDGLGAMAVIRNIDEKIVEEESYKISLGGRVIGISNYNGEKDVVISGEAEAVVQV 180 Query: 181 VEVLKNQGAQIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYEDK 240 E LK++GA+IFKLNVSAAFHSPIME AA K EL Y F P+Y+++SNV+ALPYEDK Sbjct: 181 CEELKSKGAEIFKLNVSAAFHSPIMELAAEKFKNELNRYAFKDPKYKILSNVTALPYEDK 240 Query: 241 GQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVRNN-TGIAAYSYDKQ 299 QI DIL +QIV PVNW+A MKYL + +D IEFGPKATLRNLVRNN + I AYSYDK+ Sbjct: 241 DQILDILGEQIVKPVNWHAIMKYLGQQELDNVIEFGPKATLRNLVRNNISNIGAYSYDKK 300 Query: 300 EDIEEIQAALLPEKGTSNSSSTFTLISRSLGISVATKNNNWNNEEYTRGVIEPYERIAKL 359 ED++E++ + K S+ + +++ISRSLGI+VATKNNNWNNEEY RGVI+PYE+IA L Sbjct: 301 EDVKEMKEKIFA-KEVSHQKNKYSIISRSLGIAVATKNNNWNNEEYMRGVIKPYEKIAML 359 Query: 360 QAELEETGKQPTVEQMHQAVDMLRSVFQTKQTPKEEQIERFNQLFQETNTRKYFENFSV 418 Q ELEET K PT++QM AV+ML+SVFQTK TPK+EQ ERFNQLF+ETNT++YFENF + Sbjct: 360 QTELEETNKMPTIDQMQMAVEMLKSVFQTKGTPKDEQQERFNQLFEETNTKRYFENFEI 418 >B9E2D2.1 B9E2D2_CLOK1 Putative uncharacterized protein Length = 424 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 208/419 (49%), Positives = 294/419 (70%), Gaps = 3/419 (0%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 M K+A LF GQGSQY+GMGK Y+ + IAKET+ EA++VLGFD+ +LCFEG+ +L+ TE Sbjct: 1 MKKVALLFPGQGSQYIGMGKKFYEGYPIAKETFEEANDVLGFDIRKLCFEGNMEKLTMTE 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 NTQPAIL +A +RVYMQEIG +P Y AGHSLGE +AL+ AGAI+F DAVK+VR RG + Sbjct: 61 NTQPAILTMGVAMFRVYMQEIGIEPSYLAGHSLGEITALSCAGAIQFQDAVKMVRNRGKF 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERL 180 M+ I + IE+EC+ G+++ ISNYN +V+SG AVE + Sbjct: 121 MQEATAFGTGSMAAISGLSWKEIEDECKIKREWGKLVVISNYNAWDQVVISGYKDAVEEV 180 Query: 181 VEVLKNQGAQIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYEDK 240 L +G I LNVSA FHSP+M+ A+ K EL Y +S+ ++ V+SN++ALPYED Sbjct: 181 AANLYEKGGGIIPLNVSAPFHSPLMKLASEKFENELTKYEYSKLKWPVMSNITALPYEDS 240 Query: 241 GQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVRNNTG-IAAYSYDKQ 299 +I L +QIV PV W AT++YLE+N ++ IE GPK L+NL++ N+ I YSYDK+ Sbjct: 241 SRIVKNLKKQIVEPVKWQATIEYLEKNKVEIGIELGPKTVLKNLMKKNSKRIKVYSYDKE 300 Query: 300 EDIEEIQAALLPEK--GTSNSSSTFTLISRSLGISVATKNNNWNNEEYTRGVIEPYERIA 357 ED++ ++ L + G+ + ++I+RS+ I+V TKNNNW+++EY +GVI+PY ++ Sbjct: 301 EDVQVLKTELNMNRNIGSIEKDNKMSVITRSMAIAVCTKNNNWDDKEYEKGVIQPYRKVK 360 Query: 358 KLQAELEETGKQPTVEQMHQAVDMLRSVFQTKQTPKEEQIERFNQLFQETNTRKYFENF 416 ++QAE+E+ K+PT+EQM +A+ M+RSVF TK+TP++EQIERFNQ+F ET TR F +F Sbjct: 361 EIQAEIEKEEKEPTIEQMREALKMMRSVFITKRTPQKEQIERFNQIFNETGTRHLFSDF 419 >A5N8Z3.1 A5N8Z3_CLOK5 Predicted PKS acyltransferase domain Length = 424 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 208/419 (49%), Positives = 294/419 (70%), Gaps = 3/419 (0%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 M K+A LF GQGSQY+GMGK Y+ + IAKET+ EA++VLGFD+ +LCFEG+ +L+ TE Sbjct: 1 MKKVALLFPGQGSQYIGMGKKFYEGYPIAKETFEEANDVLGFDIRKLCFEGNMEKLTMTE 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 NTQPAIL +A +RVYMQEIG +P Y AGHSLGE +AL+ AGAI+F DAVK+VR RG + Sbjct: 61 NTQPAILTMGVAMFRVYMQEIGIEPSYLAGHSLGEITALSCAGAIQFQDAVKMVRNRGKF 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERL 180 M+ I + IE+EC+ G+++ ISNYN +V+SG AVE + Sbjct: 121 MQEATAFGTGSMAAISGLSWKEIEDECKIKREWGKLVVISNYNAWDQVVISGYKDAVEEV 180 Query: 181 VEVLKNQGAQIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYEDK 240 L +G I LNVSA FHSP+M+ A+ K EL Y +S+ ++ V+SN++ALPYED Sbjct: 181 AANLYEKGGGIIPLNVSAPFHSPLMKLASEKFENELTKYEYSKLKWPVMSNITALPYEDS 240 Query: 241 GQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVRNNTG-IAAYSYDKQ 299 +I L +QIV PV W AT++YLE+N ++ IE GPK L+NL++ N+ I YSYDK+ Sbjct: 241 SRIVKNLKKQIVEPVKWQATIEYLEKNKVEIGIELGPKTVLKNLMKKNSKRIKVYSYDKE 300 Query: 300 EDIEEIQAALLPEK--GTSNSSSTFTLISRSLGISVATKNNNWNNEEYTRGVIEPYERIA 357 ED++ ++ L + G+ + ++I+RS+ I+V TKNNNW+++EY +GVI+PY ++ Sbjct: 301 EDVQVLKTELNMNRNIGSIEKDNKMSVITRSMAIAVCTKNNNWDDKEYEKGVIQPYRKVK 360 Query: 358 KLQAELEETGKQPTVEQMHQAVDMLRSVFQTKQTPKEEQIERFNQLFQETNTRKYFENF 416 ++QAE+E+ K+PT+EQM +A+ M+RSVF TK+TP++EQIERFNQ+F ET TR F +F Sbjct: 361 EIQAEIEKEEKEPTIEQMREALKMMRSVFITKRTPQKEQIERFNQIFNETGTRHLFSDF 419 >B8I5H7.1 B8I5H7_CLOCE Malonyl CoA-acyl carrier protein transacylase Length = 423 Score = 419 bits (1077), Expect = e-115, Method: Compositional matrix adjust. Identities = 208/420 (49%), Positives = 275/420 (65%), Gaps = 6/420 (1%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 M I LF GQGSQY GMGK LYDN+S+AK + EA++ LGFDL LCF GS EL++TE Sbjct: 1 MKNITLLFPGQGSQYEGMGKKLYDNYSVAKHVFDEANDALGFDLKELCFSGSLDELTKTE 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 N QPAIL SIA Y VYM+E G P Y AGHSLGE SALA AG FAD+VK+VR RG Sbjct: 61 NAQPAILTCSIAAYEVYMKEFGILPTYYAGHSLGELSALACAGIFSFADSVKIVRQRGRL 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERL 180 M+ + ++ ++++ +C S I+ ISNYN IV+SG AVE++ Sbjct: 121 MQEAAASGVGAMAAVSGIDKNILQAKCEEYSNDNNIVVISNYNSPSQIVISGHKSAVEKI 180 Query: 181 VEVLKNQGAQIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYEDK 240 L+ A + L VSA FHSP+ME K L +Y + VISNV+ LP++ K Sbjct: 181 SLELQGINAMVVPLKVSAPFHSPLMETIKGKFREVLESYPCGDFKGTVISNVTGLPHKSK 240 Query: 241 GQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNL-VRNNTGIAAYSYDKQ 299 + D+LT+Q+V PV W +TMK+L NG+ T +E GPK L NL ++N I A+SYDK Sbjct: 241 DILVDLLTEQLVQPVQWESTMKFLYENGVRTVMEIGPKTVLANLAIKNIPDIKAFSYDKP 300 Query: 300 EDIEEIQAALLPEKGTSNSSSTF---TLISRSLGISVATKNNNWNNEEYTRGVIEPYERI 356 EDIE+ + + TSN S T+++R L I+V TKN NWNNEEY +GVIEPY RI Sbjct: 301 EDIEKFRKII--TNNTSNRSENLPQPTVVTRCLAIAVCTKNKNWNNEEYQKGVIEPYRRI 358 Query: 357 AKLQAELEETGKQPTVEQMHQAVDMLRSVFQTKQTPKEEQIERFNQLFQETNTRKYFENF 416 +LQ EL++TG +P++EQM +A++ML+SVF+TK+TP +EQ+ER+NQ+F ET TR F +F Sbjct: 359 QRLQEELDKTGDKPSLEQMKEALEMLKSVFETKKTPVQEQVERYNQVFDETGTRHLFADF 418 >B8I5C8.1 B8I5C8_CLOCE Malonyl CoA-acyl carrier protein transacylase Length = 409 Score = 399 bits (1024), Expect = e-109, Method: Compositional matrix adjust. Identities = 195/411 (47%), Positives = 273/411 (66%), Gaps = 12/411 (2%) Query: 1 MSKIAFLFSGQGSQYVGMGKSLYDNFSIAKETYAEASEVLGFDLGRLCFEGSSSELSRTE 60 M+KIAFLF GQG+QY+GMG L +NF++A + + EA+E LG DL +LCF+GS +L++TE Sbjct: 1 MNKIAFLFPGQGAQYIGMGSKLCENFTVAGQVFDEANEALGLDLKKLCFQGSMDDLTKTE 60 Query: 61 NTQPAILAASIAQYRVYMQEIGEQPCYAAGHSLGEYSALAAAGAIEFADAVKLVRARGLY 120 NTQPAIL S+A +RVYMQEIG +P YAAGHSLGE SAL AG IEF DAV LVR RG + Sbjct: 61 NTQPAILTVSVAAFRVYMQEIGVKPDYAAGHSLGELSALCCAGGIEFTDAVSLVRQRGRF 120 Query: 121 MKXXXXXXXXXXXXIRDVEDSVIEEECRTLSAQGRIIGISNYNGNKDIVVSGEAQAVERL 180 M+ + VE +IE EC +S G I+ +SNYN + V+SG +AV + Sbjct: 121 MQEAVPEGTGSMAAVSGVEKEIIERECSLVSKGGNIVVVSNYNSLEQTVISGHVKAVNDV 180 Query: 181 VEVLKNQGAQIFKLNVSAAFHSPIMEPAAVKLNRELGNYNFSQPQYQVISNVSALPYEDK 240 E LK+ GA++ L VSA FHSP+M+PAA + L Y+F + ++ VISNV++ PY K Sbjct: 181 GEQLKSMGARVVYLKVSAPFHSPLMQPAADRFRDVLKKYSFGEMKWPVISNVTSEPYPGK 240 Query: 241 GQIRDILTQQIVNPVNWYATMKYLERNGIDTAIEFGPKATLRNLVRNNTGIAAYSYDKQE 300 +I D L+ QI NPV W +TM+YL+ +GID A+E GPKA L+NL+R+N I +S + E Sbjct: 241 DKIIDYLSLQITNPVRWQSTMEYLQNSGIDKAVEMGPKAVLKNLLRSNKSITVFSSETTE 300 Query: 301 DIEEIQAALLPEKGTSNSSST-------FTLISRSLGISVATKNNNWNNEEYTRGVIEPY 353 DI +L+ +K S++S + +I+R + +V+TKN NW+ Y +GV+EPY Sbjct: 301 DI-----SLIKKKFDSDASGSKKPANNGLMVITRCIAAAVSTKNRNWDENAYQKGVVEPY 355 Query: 354 ERIAKLQAELEETGKQPTVEQMHQAVDMLRSVFQTKQTPKEEQIERFNQLF 404 ++ +++ EL + QPT E + +AVD+L+ +F TK EEQ ER +QLF Sbjct: 356 RKLVEMKEELVKQRIQPTNEHIEEAVDLLKQIFNTKLLTIEEQNERLSQLF 406 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.130 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,222,999,383 Number of extensions: 87494176 Number of successful extensions: 228539 Number of sequences better than 1.0e-05: 4328 Number of HSP's gapped: 218445 Number of HSP's successfully gapped: 5031 Length of query: 418 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 280 Effective length of database: 1,760,596,133 Effective search space: 492966917240 Effective search space used: 492966917240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)