BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0028.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3HA43.1 C3HA43_BACTU FAD dependent oxidoreductase 418 e-115 B5IFV0.1 B5IFV0_9EURY FAD dependent oxidoreductase, putative 181 9e-44 B5H419.1 B5H419_STRCL FAD dependent oxidoreductase 172 3e-41 Q2FMH2.1 Q2FMH2_METHJ FAD dependent oxidoreductase 168 7e-40 C3PTT5.1 C3PTT5_LYSEN Ox1 167 2e-39 >C3HA43.1 C3HA43_BACTU FAD dependent oxidoreductase Length = 517 Score = 418 bits (1074), Expect = e-115, Method: Compositional matrix adjust. Identities = 191/277 (68%), Positives = 227/277 (81%) Query: 1 MDNHTTXXXXXXXXXXXXXCYLQMNGYDTMIMEAGALPGGLCTGWKRKGYYFDGCISWLV 60 M N T CYLQMNGY T I E G LPGGLCTGW RKGYYFDGCISWLV Sbjct: 7 MRNKTAIIIGAGVAGMAAGCYLQMNGYKTKIYEMGLLPGGLCTGWSRKGYYFDGCISWLV 66 Query: 61 GSGPNNTFHKFWSELNVIQNMEFVNDEEFAVVNFKDGTKFTLYADVDKLEQELLRVAPED 120 GSGPNNTFHKFW ELNVIQNMEF+NDEEFAVVNFKDG+ FTLY+DVDKLEQE LRVAPED Sbjct: 67 GSGPNNTFHKFWKELNVIQNMEFINDEEFAVVNFKDGSTFTLYSDVDKLEQEFLRVAPED 126 Query: 121 AALIKILTDAIKVISSVDLRIDKAPELFDLSDFQAFKAKAGEYMEVMKKWGSVTIEEFAL 180 IK+LTDAI+VISSVDL +DKAPELFDLSDF+ FKAKAG YM+VMK+WG VTIEEF+ Sbjct: 127 EETIKVLTDAIRVISSVDLDLDKAPELFDLSDFERFKAKAGPYMKVMKEWGKVTIEEFSK 186 Query: 181 QFKSEFVRTNYRKIFWFNVDTAVAYFMATMGWVNAKASGYPVDSSMQLSRIIEQRYMGLG 240 +FKS VR N+ +IFWFN+DT+++YF+ATMGWVN+K++G+PV+ S+Q ++IIE+RY+ LG Sbjct: 187 RFKSPLVRENFSRIFWFNIDTSISYFIATMGWVNSKSAGHPVEGSLQFAKIIEKRYVDLG 246 Query: 241 GKIHYQSKVSRILTAEDKVTGVELDNGRIYHADLVIS 277 G+IHY+SKV+ IL ++K G++L NG+ Y D VIS Sbjct: 247 GEIHYKSKVANILVEDNKAIGIKLKNGQTYKGDFVIS 283 >B5IFV0.1 B5IFV0_9EURY FAD dependent oxidoreductase, putative Length = 503 Score = 181 bits (458), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 94/258 (36%), Positives = 144/258 (55%), Gaps = 1/258 (0%) Query: 20 CYLQMNGYDTMIMEAGALPGGLCTGWKRKGYYFDGCISWLVGSGPNNTFHKFWSELNVIQ 79 CY +MNGY + E LPGGLCT WKR GY DG I WL+GS P +F++ W EL +Q Sbjct: 19 CYARMNGYAAEVYEMHDLPGGLCTSWKRYGYTIDGSIHWLMGSKPGTSFYRLWEELGAVQ 78 Query: 80 NMEFVNDEEFAVVNFKDGTKFTLYADVDKLEQELLRVAPEDAALIKILTDAIKVISSVDL 139 N + + F + G + T Y + DKLE+EL PED I++L + I+ S+ + Sbjct: 79 NRKMYYHDVFYIFTDNQGQEITWYRNADKLEEELKSKFPEDKENIELLCNLIRKFSNFPM 138 Query: 140 RIDKAPELFDLSDFQAFKAKAGEYMEVMKKWGSVTIEEFALQFKSEFVRTNYRKIFWFNV 199 IDK EL+++ DF + + +K +TI EFA +F + +R I Sbjct: 139 PIDKPYELYNIFDFFKMMKIMRPFFKDLKFSSQITIGEFAERFSNPVLRELVSLILG-ER 197 Query: 200 DTAVAYFMATMGWVNAKASGYPVDSSMQLSRIIEQRYMGLGGKIHYQSKVSRILTAEDKV 259 D + + T+G ++A +P S++ ++ IE+R + LG KIHY+ KV++I+ ++ K Sbjct: 198 DFPLISLLVTLGAMHAGDGSFPQGGSLEFAKAIEKRCLELGAKIHYRRKVTKIIVSKGKA 257 Query: 260 TGVELDNGRIYHADLVIS 277 G+EL+NG + D VIS Sbjct: 258 VGIELENGEKIYGDYVIS 275 >B5H419.1 B5H419_STRCL FAD dependent oxidoreductase Length = 569 Score = 172 bits (437), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 86/269 (31%), Positives = 140/269 (52%), Gaps = 20/269 (7%) Query: 20 CYLQMNGYDTMIMEAGALPGGLCTGWKRKGYYFDGCISWLVGSGPNNTFHKFWSELNVIQ 79 CY QMNG + I E LPGG CT W R GY FD CI WL+G+ P N H+ W EL + Sbjct: 42 CYAQMNGMRSRIFEKHVLPGGCCTAWGRDGYVFDYCIEWLIGTAPGNEAHRIWRELGALD 101 Query: 80 NMEFVNDEEFAVVNFKDGTKFTLYADVDKLEQELLRVAPEDAALIKILTDAIKVISSVDL 139 + + F V + G Y D +LE LL ++P DA LI+ ++ + + Sbjct: 102 GKSITHFDMFNRVTDESGRSVMFYNDPGRLEAHLLGISPADAPLIRSFCRDLRRFTDI-- 159 Query: 140 RIDKAPELFDLSDFQAFKAKAGEYMEVMK----------KWGSVTIEEFALQFKSEFVRT 189 EL+ A ++ AGE + +++ + + + FA +F+ +R Sbjct: 160 ------ELYPFLTAPALRS-AGERLRMLRTVLPAFRLFWRTAATPMHAFADRFEDPLLRR 212 Query: 190 NYRKIFWFNVDT-AVAYFMATMGWVNAKASGYPVDSSMQLSRIIEQRYMGLGGKIHYQSK 248 +R +F+ + + + ++ M + + +G+P S+ L+R IE+RY LGG + Y+S+ Sbjct: 213 AFRNVFFQDPEGFPLLPYLFNMAAAHRRNAGFPQGGSLGLARSIEERYTALGGTVSYRSR 272 Query: 249 VSRILTAEDKVTGVELDNGRIYHADLVIS 277 V+R+L D+ G+EL NGR Y+AD V++ Sbjct: 273 VARVLVERDRAVGIELGNGRRYYADHVVA 301 >Q2FMH2.1 Q2FMH2_METHJ FAD dependent oxidoreductase Length = 484 Score = 168 bits (425), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 92/273 (33%), Positives = 147/273 (53%), Gaps = 15/273 (5%) Query: 20 CYLQMNGYDTMIMEAGALPGGLCTGWKRKGYYFDGCISWLVGSGPNNTFHKFWSELNVIQ 79 C+ +MNG++T I+E +PGGLCT W RKGY FD CI WL GS P ++ + +L +++ Sbjct: 19 CFARMNGFETEILEMHTIPGGLCTAWTRKGYTFDLCIHWLCGSKPGTPLYQVYDKLGLME 78 Query: 80 NMEFVNDEEFAVVNFKDGTKFTLYADVDKLEQELLRVAPEDAALIKILTDAIKVISSVDL 139 F + + + V + G + T+Y D D+L EL+R++PED I L IK +S ++ Sbjct: 79 GRRFHHTDSWTSVRDEHGNEITIYTDPDRLYDELIRISPEDTPFISRLCKDIKKLSRMET 138 Query: 140 RIDKAPELFDLSDFQAFKAKAGEYMEVMKKWGSVTIEEFALQFKSEFVRTNYRKIFWFNV 199 +D + D+ + Y +MK++ T+ + + KS +R F + Sbjct: 139 LVDMT--ILDIIRYIP-------YFSLMKRY-KPTVHDILSEVKSPVLRNLLTAGFGWE- 187 Query: 200 DTAVAYFMATMGWV--NAKASGYPVDSSMQLSRIIEQRYMGLGGKIHYQSKVSRILTAED 257 + F MG AK +GYP+ S+ ++ IE+RY+ LGG I Y+SKV+ I+T ++ Sbjct: 188 --GQSLFFPLMGLAMQGAKNAGYPIGGSLPCAKAIEKRYLDLGGVIRYKSKVTAIITEKN 245 Query: 258 KVTGVELDNGRIYHADLVISXXXXGVSARRWLQ 290 + GV L +G +AD VIS + WLQ Sbjct: 246 RAKGVRLADGTERYADYVISCADGHTTIFEWLQ 278 >C3PTT5.1 C3PTT5_LYSEN Ox1 Length = 547 Score = 167 bits (422), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 90/260 (34%), Positives = 136/260 (52%), Gaps = 2/260 (0%) Query: 20 CYLQMNGYDTMIMEAGALPGGLCTGWKRKGYYFDGCISWLVGSGPNNTFHKFWSELNVIQ 79 CY QM+G +T I E LPGG CT W R GY FD CI WL G+ P N ++ W EL + Sbjct: 8 CYAQMSGIETRIFEKHVLPGGCCTAWSRGGYIFDYCIEWLNGTAPGNNANQVWRELGALD 67 Query: 80 NMEFVNDEEFAVVNFKDGTKFTLYADVDKLEQELLRVAPEDAALIKILTDAIKVISSVDL 139 N E F V + G + Y D D+LE+EL+ V+P+DA LIK + + + L Sbjct: 68 GKTVRNFELFNKVVDERGREVNFYNDPDRLERELIAVSPQDAPLIKAFCADFRRFTKLAL 127 Query: 140 R-IDKAPELFDLSDFQAFKAKAGEYMEVMKKWGSVTIEEFALQFKSEFVRTNYRKIFWFN 198 K P L + + A + + + G+ + +A +F +R +R +F+ + Sbjct: 128 HPFLKPPPLENWKEKLATLRQVLPAFALFWRTGAAQMGPYADRFADPLLRRGFRNMFFQD 187 Query: 199 VDT-AVAYFMATMGWVNAKASGYPVDSSMQLSRIIEQRYMGLGGKIHYQSKVSRILTAED 257 AV F+ + G+P S+ LSR +E+RY+GLGG I Y+++V R+L + Sbjct: 188 PGNFAVLPFLYNLAEAYNDNVGFPQGGSLGLSRSVEERYLGLGGHIAYRTRVDRVLVEDG 247 Query: 258 KVTGVELDNGRIYHADLVIS 277 + GV L NG ++AD V+S Sbjct: 248 RAVGVRLKNGECHYADHVVS 267 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,640,065,519 Number of extensions: 65014398 Number of successful extensions: 147950 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 147947 Number of HSP's successfully gapped: 41 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)