BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0382.0002.p (338 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEK2.1 B3KEK2_9BACI Metallophosphoesterase 342 2e-92 B1DA18.1 B1DA18_9BACL Metallophosphoesterase 326 2e-87 C2RQ96.1 C2RQ96_BACCE Phosphohydrolase 91 1e-16 C2YCK3.1 C2YCK3_BACCE Phosphohydrolase 91 2e-16 C3FMB3.1 C3FMB3_BACTB Phosphohydrolase 89 6e-16 >B3KEK2.1 B3KEK2_9BACI Metallophosphoesterase Length = 292 Score = 342 bits (878), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 172/326 (52%), Positives = 201/326 (61%), Gaps = 40/326 (12%) Query: 8 SRMSTNESGGQQPLLTFQIITDTHVTADPSHEYNLNFGRALQDLALHAQGSSGIMHIGDI 67 R E+ LL FQ+ITDTHV ADP H YN N RAL+D+ A S GIMH GD+ Sbjct: 3 KRNQDGENRKGDVLLRFQVITDTHVKADPGHTYNCNLARALEDIKRMAPDSDGIMHAGDL 62 Query: 68 TDHGFTEEYEEVHRILQLHQAALPEIRYTLGNHDVGLGHWESRLAMYTSRTGMTGPYHDH 127 TD G TEEY E RI + H A LP + GNHDVGLGHW SRL Y TGM GPYHDH Sbjct: 63 TDQGSTEEYAEFRRIWREHGAGLPVSYFAAGNHDVGLGHWPSRLGAYLDATGMRGPYHDH 122 Query: 128 WICGYHFIFLGTEEGLPTFCNLSEDQLRWLDRKLGERAAEARELGRQLTDPLELSGQTVV 187 W+ GYHFIFLGTEEGL F +LSE QL WLD KL E Sbjct: 123 WVNGYHFIFLGTEEGLELFASLSETQLTWLDTKLTE------------------------ 158 Query: 188 EHEPGRLPVSVQAFDDTAAQSQPIFLFLHQPLKDTVAGSLESQEWYGVTQDKELRAILSK 247 + PV QP F+FLHQPLKDTVAGS ESQ WYGV QD+ L+ +L+K Sbjct: 159 ----SKNPV------------QPAFVFLHQPLKDTVAGSYESQRWYGVAQDEALKKVLAK 202 Query: 248 YPQTLLFTGHTHWELEVSNTMYPGNGETATMFNAASVAYLWTNADEHKSGSQGYYVEVYC 307 + +LFTGHTHWELE +TM+ G MFN +SVAYLWT+ DEHK GSQG +VEVY Sbjct: 203 HRHAILFTGHTHWELEAQHTMFDGQERLPYMFNGSSVAYLWTDKDEHKDGSQGLFVEVYA 262 Query: 308 DKVLVRGRDFTTGSWIESAQYEVATP 333 D+V+V+GRDF G W+E+ ++E P Sbjct: 263 DRVIVKGRDFAAGKWLENVRFECNIP 288 >B1DA18.1 B1DA18_9BACL Metallophosphoesterase Length = 283 Score = 326 bits (836), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 166/321 (51%), Positives = 201/321 (62%), Gaps = 41/321 (12%) Query: 13 NESGGQQPLLTFQIITDTHVTADPSHEYNLNFGRALQDLALHAQGSSGIMHIGDITDHGF 72 +E G ++ L FQ+ITDTHV DP H +N N RAL+D+ A S GIMH+GDITDHG Sbjct: 3 SEEGKEKAKLQFQVITDTHVRTDPGHTHNGNLARALEDMKREAPDSDGIMHVGDITDHGH 62 Query: 73 TEEYEEVHRILQLHQAALPEIRYTLGNHDVGLGHWESRLAMYTSRTGMTGPYHDHWICGY 132 EEYEE +I + H LP + GNHDVG G W SRL Y + T M GPYHDHW+ GY Sbjct: 63 AEEYEEFRKIWREHGEGLPAAYFASGNHDVGGGEWPSRLGAYLNATEMGGPYHDHWVKGY 122 Query: 133 HFIFLGTEEGLPTFCNLSEDQLRWLDRKLGERAAEARELGRQLTDPLELSGQTVVEHEPG 192 FIFLGTE+GL F LSE QL WLD KL E++ L R Sbjct: 123 SFIFLGTEQGLDLFSFLSEAQLSWLDGKL----EESKSLNR------------------- 159 Query: 193 RLPVSVQAFDDTAAQSQPIFLFLHQPLKDTVAGSLESQEWYGVTQDKELRAILSKYPQTL 252 P F+FLHQPLKDTVAGS ESQ WYGVTQD++L+ +LS++ + Sbjct: 160 -----------------PAFVFLHQPLKDTVAGSYESQNWYGVTQDEQLKEVLSRHRHAI 202 Query: 253 LFTGHTHWELEVSNTMYPGNGETATMFNAASVAYLWTNADEHKSGSQGYYVEVYCDKVLV 312 LFTGHTHWELE +T+Y + + MFNAASVAYLWT+ DEHK GSQG +VEVY D+++V Sbjct: 203 LFTGHTHWELEAPHTLYD-DEQLPRMFNAASVAYLWTDEDEHKDGSQGLFVEVYEDRIIV 261 Query: 313 RGRDFTTGSWIESAQYEVATP 333 RGRDF G W+E +YE P Sbjct: 262 RGRDFAAGQWLEKVRYEWEIP 282 >C2RQ96.1 C2RQ96_BACCE Phosphohydrolase Length = 410 Score = 91.3 bits (225), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 84/334 (25%), Positives = 136/334 (40%), Gaps = 86/334 (25%) Query: 23 TFQIITDTHVTADPSHEYNLNFGRALQDLALHAQGSSGIMHIGDITDHGFTEEYEEVHRI 82 TF +I+D + D +F L+D+ S ++ GDIT G +Y++V R+ Sbjct: 34 TFNVISD--IQGDLG-----DFDHVLKDMNKVTPLSRALIMNGDITPTGQQSQYDDVKRV 86 Query: 83 LQLHQAALPE-IRYTLGNHDVGLGHW-----------------ESRLAMYTSRTGMTGPY 124 L ++ PE + T+GNH+ G W E+ Y +G Y Sbjct: 87 LNKNKH--PENVWSTVGNHEFYAGKWTADGKLSQSTWPNGVTEETLFGRYLQFSGQEKVY 144 Query: 125 HDHWICGYHFIFLGTEEGLPTFCN-------LSEDQLRWLDRKLGERAAEARELGRQLTD 177 H + GY F+FLGTE+ + + +S++QL WL + L E + + + Sbjct: 145 HKKELDGYPFLFLGTEKYMKYHDSKMWDEVYMSDEQLGWLKQNLEEYSQKDK-------- 196 Query: 178 PLELSGQTVVEHEPGRLPVSVQAFDDTAAQSQPIFLFLHQPLKDTVAGSLESQEWYGVTQ 237 ++PIF+F H L DTV+GS +S Sbjct: 197 ------------------------------NKPIFIFSHHVLPDTVSGSRQSPYLQDYLN 226 Query: 238 DKELRAILSKYPQTLLFTGHTHWELEVSN-----TMYPGNGETATMFNAASVAYLWTNAD 292 +L IL YPQ + FT HTHW+L + + + G+ + T+ N + W +A Sbjct: 227 VDKLYDILKDYPQVVFFTSHTHWDLNLPDWAGKKKIKGGDEKGFTVVNTGGIETGWMSAG 286 Query: 293 EHK---------SGSQGYYVEVYCDKVLVRGRDF 317 + S QG V+ Y + V+V D+ Sbjct: 287 PNGGEKTAPDGYSFKQGLQVKAYGNDVIVTAYDY 320 >C2YCK3.1 C2YCK3_BACCE Phosphohydrolase Length = 410 Score = 90.9 bits (224), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 84/334 (25%), Positives = 135/334 (40%), Gaps = 86/334 (25%) Query: 23 TFQIITDTHVTADPSHEYNLNFGRALQDLALHAQGSSGIMHIGDITDHGFTEEYEEVHRI 82 TF +I+D + D +F L+D+ S ++ GDIT G +Y++V R+ Sbjct: 34 TFNVISD--IQGDLG-----DFDHVLKDMNKVTPLSRALIMNGDITPTGQQSQYDDVKRV 86 Query: 83 LQLHQAALPE-IRYTLGNHDVGLGHW-----------------ESRLAMYTSRTGMTGPY 124 L ++ PE + T+GNH+ G W E+ Y +G Y Sbjct: 87 LNKNKH--PENVWSTVGNHEFYAGKWTADGKLSQSTWPNGVNEETLFNRYLKFSGQEKVY 144 Query: 125 HDHWICGYHFIFLGTEEGLPTF-------CNLSEDQLRWLDRKLGERAAEARELGRQLTD 177 H + GY F+FLGTE+ + +S++QL WL + L E + + + Sbjct: 145 HKKELDGYPFLFLGTEKYMKYHDSKMWDEVYMSDEQLGWLKQNLEEYSQKDK-------- 196 Query: 178 PLELSGQTVVEHEPGRLPVSVQAFDDTAAQSQPIFLFLHQPLKDTVAGSLESQEWYGVTQ 237 ++PIF+F H L DTV+GS +S Sbjct: 197 ------------------------------NKPIFIFSHHVLPDTVSGSRQSPYLQDYLN 226 Query: 238 DKELRAILSKYPQTLLFTGHTHWELEVSN-----TMYPGNGETATMFNAASVAYLWTNAD 292 +L IL YPQ + FT HTHW+L + + + G+ + T+ N + W +A Sbjct: 227 VDKLYDILKDYPQVVFFTSHTHWDLNLPDWAGKKKIAGGDEKGFTVVNTGGIETGWMSAG 286 Query: 293 EHK---------SGSQGYYVEVYCDKVLVRGRDF 317 + S QG V+ Y + V+V D+ Sbjct: 287 PNGGEKTAPDGYSFKQGLQVKAYGNDVIVTAYDY 320 >C3FMB3.1 C3FMB3_BACTB Phosphohydrolase Length = 410 Score = 89.0 bits (219), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 83/334 (24%), Positives = 135/334 (40%), Gaps = 86/334 (25%) Query: 23 TFQIITDTHVTADPSHEYNLNFGRALQDLALHAQGSSGIMHIGDITDHGFTEEYEEVHRI 82 TF +I+D + D +F L+D+ S ++ GDIT G +Y++V R+ Sbjct: 34 TFNVISD--IQGDLG-----DFDHVLKDMNKVTPLSRALIMNGDITPTGQQSQYDDVKRV 86 Query: 83 LQLHQAALPE-IRYTLGNHDVGLGHW-----------------ESRLAMYTSRTGMTGPY 124 L ++ PE + T+GNH+ G W E+ Y +G Y Sbjct: 87 LNKNKH--PENVWSTVGNHEFYAGKWTADGKLSQSTWPNGVTEETLFGRYLQFSGQEKVY 144 Query: 125 HDHWICGYHFIFLGTEEGLPTFCN-------LSEDQLRWLDRKLGERAAEARELGRQLTD 177 H + GY +FLGTE+ + + +S++QL WL + L E + + + Sbjct: 145 HKKELDGYPLLFLGTEKYMKYHDSKMWDEVYMSDEQLGWLKQNLEEYSQKDK-------- 196 Query: 178 PLELSGQTVVEHEPGRLPVSVQAFDDTAAQSQPIFLFLHQPLKDTVAGSLESQEWYGVTQ 237 ++PIF+F H L DTV+GS +S Sbjct: 197 ------------------------------NKPIFIFSHHVLPDTVSGSRQSPYLQDYLN 226 Query: 238 DKELRAILSKYPQTLLFTGHTHWELEVSN-----TMYPGNGETATMFNAASVAYLWTNAD 292 +L IL YPQ + FT HTHW+L + + + G+ + T+ N + W +A Sbjct: 227 VDKLYDILKDYPQVVFFTSHTHWDLNLPDWAGKKKIKGGDEKGFTVVNTGGIETGWMSAG 286 Query: 293 EHK---------SGSQGYYVEVYCDKVLVRGRDF 317 + S QG V+ Y + V+V D+ Sbjct: 287 PNGGEKTAPDGYSFKQGLQVKAYGNDVIVTAYDY 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,124,668,613 Number of extensions: 89048963 Number of successful extensions: 179160 Number of sequences better than 1.0e-05: 150 Number of HSP's gapped: 178947 Number of HSP's successfully gapped: 226 Length of query: 338 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 202 Effective length of database: 1,779,438,163 Effective search space: 359446508926 Effective search space used: 359446508926 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)