BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0378.0006.p (465 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBN6.1 B3KBN6_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 725 0.0 B1DDX7.1 B1DDX7_9BACL DNA polymerase III, alpha subunit EC=2.7.7.7 669 0.0 Q2RHB7.1 Q2RHB7_MOOTA DNA polymerase III catalytic subunit, DnaE... 535 e-150 B0K6L0.1 B0K6L0_THEPX DNA polymerase III, alpha subunit EC=2.7.7.7 529 e-148 B0K7U5.1 B0K7U5_THEP3 DNA polymerase III, alpha subunit EC=2.7.7.7 527 e-148 >B3KBN6.1 B3KBN6_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 Length = 1241 Score = 725 bits (1871), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/450 (77%), Positives = 383/450 (85%), Gaps = 10/450 (2%) Query: 1 MSPFVHLHVHSEYSLLDGAARITDLVRRAGEYGMTTLALTDHGVMYGAIPFYKACKASGI 60 MS FVHLHVHSEYSLLDGAARI DLVR A GM +LALTDHGVMYGA+PFYKAC A GI Sbjct: 1 MSSFVHLHVHSEYSLLDGAARIGDLVREAAALGMKSLALTDHGVMYGAVPFYKACLAQGI 60 Query: 61 KPIIGWEAXXXGCEAYLTAGSRRERGSRKDQPIYHLILLAKNETGYRNLMRLVTIGHLEG 120 KPIIG CEAY+TAGSR+ERGSRKDQPIYHLILLAKNETGYRNLM+L +IGHLEG Sbjct: 61 KPIIG-------CEAYITAGSRKERGSRKDQPIYHLILLAKNETGYRNLMKLCSIGHLEG 113 Query: 121 QHYKPRIDMESLAAHAEGVICLXXXXACLGGEVPQHLLHGREEEARKAALRYKEIFGGDF 180 HY+PRIDME+LAAH EG+ICL ACLGGEVPQHLLHGRE EA+ AALRY+ IFG D+ Sbjct: 114 YHYRPRIDMEALAAHHEGIICLS---ACLGGEVPQHLLHGREAEAKAAALRYQAIFGEDY 170 Query: 181 YLELQDHGMPEQKRVNPQLIALAAECGIPLVATNDVHYLAKEDAEVQDVLICIGTGKTVD 240 YLELQDHGMPEQKRVNPQLI L+ E GIPLVATNDVHYL + DAEVQDVLICIGTGKTV+ Sbjct: 171 YLELQDHGMPEQKRVNPQLIQLSRETGIPLVATNDVHYLTENDAEVQDVLICIGTGKTVE 230 Query: 241 DEERLKIGTDQLFLKSGEQMAALFPHVPEAIANTLNIAEKCNLELTFGEHILPAYSPLPE 300 DE+RL+I T+QL+LKSG M L+PHVPEAI NT IA++C+LEL FG+ ILP YS LPE Sbjct: 231 DEDRLQIQTNQLYLKSGSDMERLYPHVPEAILNTAKIADQCHLELEFGKSILPEYSSLPE 290 Query: 301 GMDAAAYLRELCWSGLEARYADTPRWSSPEEKDAAGKRLAYELGVIESMGFSDYFLIVWD 360 G +AA YLR+LC SGL RY +T RW ++ +RL YELGVIESMGFSDYFLIVWD Sbjct: 291 GKNAAEYLRDLCLSGLRERYEETERWQDEVQRSELEQRLDYELGVIESMGFSDYFLIVWD 350 Query: 361 FIAFCHRQGIVTGPGRGSSAGSLTAYCLRITDVDPLKYNLLFERFLNPERITMPDIDIDF 420 FIA+ HR+GI GPGRGSSAGSL AY L+IT+VDP+KY LLFERFLNPERITMPDIDIDF Sbjct: 351 FIAYAHRKGIAVGPGRGSSAGSLVAYTLQITNVDPMKYKLLFERFLNPERITMPDIDIDF 410 Query: 421 SDERRDEVIAYVVDKYGKQHVAQIITFGTM 450 SDERRDEVI YVVDKYGK+HVAQIITFGTM Sbjct: 411 SDERRDEVIDYVVDKYGKEHVAQIITFGTM 440 >B1DDX7.1 B1DDX7_9BACL DNA polymerase III, alpha subunit EC=2.7.7.7 Length = 1220 Score = 669 bits (1726), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/450 (73%), Positives = 364/450 (80%), Gaps = 11/450 (2%) Query: 2 SPFVHLHVHSEYSLLDGAARITDLVRRAGEYGMTTLALTDHGVMYGAIPFYKACKASGIK 61 S FVHLHVHSEYSLLDGAARI DLV RA E GM LALTDHGVMYGAIPFY+AC A GIK Sbjct: 6 SSFVHLHVHSEYSLLDGAARIRDLVARAAEQGMKALALTDHGVMYGAIPFYRACLARGIK 65 Query: 62 PIIGWEAXXXGCEAYLTAGSRRERGSRKDQPIYHLILLAKNETGYRNLMRLVTIGHLEGQ 121 PIIG CE Y TAGSR E+G+RKD PIYHLILLAKNETGYRNLM+LV+IGHLEG Sbjct: 66 PIIG-------CEMYFTAGSRFEKGTRKDNPIYHLILLAKNETGYRNLMKLVSIGHLEGF 118 Query: 122 HYKPRIDMESLAAHAEGVICLXXXXACLGGEVPQHLLHGREEEARKAALRYKEIFGGDFY 181 HYKPRID E L AH+EG++CL +CL GE+ QHLLH R EEA AA+RYK IFG DFY Sbjct: 119 HYKPRIDPEVLEAHSEGLVCLS---SCLHGEISQHLLHDRREEALTAAMRYKRIFGDDFY 175 Query: 182 LELQDHGMPEQKRVNPQLIALAAECGIPLVATNDVHYLAKEDAEVQDVLICIGTGKTVDD 241 LELQDHGM +QK+V +I LA E GI L ATNDVHYL EDA VQDVLICIGTGK+VDD Sbjct: 176 LELQDHGMLDQKKVAAAMIELARETGIKLTATNDVHYLTPEDAAVQDVLICIGTGKSVDD 235 Query: 242 EERLKIGTDQLFLKSGEQMAALFPHVPEAIANTLNIAEKCNLELTFGEHILPAYSPLPEG 301 +R+K+GTDQL++KSGE+M LF HVPEAI +T IA+KC+LELTFG LP + P+P G Sbjct: 236 TDRMKMGTDQLYMKSGEEMELLFRHVPEAIRHTAEIADKCSLELTFGRAALPVFRPVPGG 295 Query: 302 MDAAAYLRELCWSGLEARYADTPRWS-SPEEKDAAGKRLAYELGVIESMGFSDYFLIVWD 360 M + AYL ELC SGL +RYA P+WS P ++ A RLAYEL VIE+MGFSDYFLIVWD Sbjct: 296 MTSTAYLAELCRSGLASRYAGLPQWSEDPAYREQAEARLAYELSVIENMGFSDYFLIVWD 355 Query: 361 FIAFCHRQGIVTGPGRGSSAGSLTAYCLRITDVDPLKYNLLFERFLNPERITMPDIDIDF 420 F+ F H QGI TGPGRGSSAGSL AY LRITDVDPLKY LLFERFLNPERITMPDIDIDF Sbjct: 356 FVRFAHEQGIRTGPGRGSSAGSLVAYVLRITDVDPLKYKLLFERFLNPERITMPDIDIDF 415 Query: 421 SDERRDEVIAYVVDKYGKQHVAQIITFGTM 450 +DERRDEVIAYV KYG +HVAQIITFGTM Sbjct: 416 NDERRDEVIAYVSAKYGDEHVAQIITFGTM 445 >Q2RHB7.1 Q2RHB7_MOOTA DNA polymerase III catalytic subunit, DnaE type Length = 1156 Score = 535 bits (1377), Expect = e-150, Method: Compositional matrix adjust. Identities = 269/451 (59%), Positives = 325/451 (72%), Gaps = 20/451 (4%) Query: 1 MSPFVHLHVHSEYSLLDGAARITDLVRRAGEYGMTTLALTDHGVMYGAIPFYKACKASGI 60 M FVHLHVHSEYSLLDGA RI DLVR AGE GM LALTDHGVMYGA+ FYKA + GI Sbjct: 1 MKSFVHLHVHSEYSLLDGAGRIKDLVRAAGEMGMPALALTDHGVMYGAVEFYKAAREQGI 60 Query: 61 KPIIGWEAXXXGCEAYLTAGSRRERGSRKDQPIYHLILLAKNETGYRNLMRLVTIGHLEG 120 KPIIG CE Y+ SR +R +D YHL+LLA + TGYRNL LV+ LEG Sbjct: 61 KPIIG-------CEVYVAPRSRHDREPHRDDYQYHLVLLATDATGYRNLTALVSAAFLEG 113 Query: 121 QHYKPRIDMESLAAHAEGVICLXXXXACLGGEVPQHLLHGREEEARKAALRYKEIFGG-D 179 +YKPR+D E L+ H++G+I L ACL GEVP HLL RE+ A +AA +E+FG + Sbjct: 114 FYYKPRVDRELLSRHSQGLIALS---ACLAGEVPAHLLKNREDAAYEAATWLREVFGPEN 170 Query: 180 FYLELQDHGMPEQKRVNPQLIALAAECGIPLVATNDVHYLAKEDAEVQDVLICIGTGKTV 239 FYLELQD G+PEQ+++N +L+ LAA IPLVATNDVHY+ ++ A DVL+CI TGKT+ Sbjct: 171 FYLELQDQGLPEQRQLNRRLVDLAARLKIPLVATNDVHYIRQDQARAHDVLLCIQTGKTL 230 Query: 240 DDEERLKIGTDQLFLKSGEQMAALFPHVPEAIANTLNIAEKCNLELTFGEHILPAYSPLP 299 DD RL+ T Q +LKS +M LF VP A+ANTL IAE+C+ + TFG LPAY +P Sbjct: 231 DDPNRLRFPTAQFYLKSPAEMDNLFREVPSALANTLAIAERCHFDFTFGRLHLPAYQ-VP 289 Query: 300 EGMDAAAYLRELCWSGLEARYADTPRWSSPEEKDAAGKRLAYELGVIESMGFSDYFLIVW 359 G DAA+YLR L + GL+ RY P + A +RL YELG+IE MG+ YFLIVW Sbjct: 290 AGEDAASYLRRLSYEGLKRRY--------PHDDGTARQRLEYELGIIEQMGYPGYFLIVW 341 Query: 360 DFIAFCHRQGIVTGPGRGSSAGSLTAYCLRITDVDPLKYNLLFERFLNPERITMPDIDID 419 D + F R+GI GPGRGS+AGSL AYCL IT +DPL+YNLLFERFLNPER++MPDID+D Sbjct: 342 DMVNFARRRGIPVGPGRGSAAGSLVAYCLGITSIDPLRYNLLFERFLNPERVSMPDIDMD 401 Query: 420 FSDERRDEVIAYVVDKYGKQHVAQIITFGTM 450 F ERRDEVI YV++KYG+ HVAQIITFGTM Sbjct: 402 FCFERRDEVIQYVLEKYGRDHVAQIITFGTM 432 >B0K6L0.1 B0K6L0_THEPX DNA polymerase III, alpha subunit EC=2.7.7.7 Length = 1145 Score = 529 bits (1362), Expect = e-148, Method: Compositional matrix adjust. Identities = 263/448 (58%), Positives = 321/448 (71%), Gaps = 20/448 (4%) Query: 4 FVHLHVHSEYSLLDGAARITDLVRRAGEYGMTTLALTDHGVMYGAIPFYKACKASGIKPI 63 FVHLHVHSEYSLLDG+ RI DL+ + E M +A+TDHGVMYG I FYK A GIKPI Sbjct: 2 FVHLHVHSEYSLLDGSCRIKDLIAKTKELNMKAIAITDHGVMYGVIDFYKEAVAQGIKPI 61 Query: 64 IGWEAXXXGCEAYLTAGSRRERGSRKDQPIYHLILLAKNETGYRNLMRLVTIGHLEGQHY 123 IG CE Y+ S ++R D YHL+LLAK+ TGY+NLM++VT+ +EG +Y Sbjct: 62 IG-------CEIYVAPRSMQDREYGIDNENYHLVLLAKDMTGYKNLMKIVTVASIEGFYY 114 Query: 124 KPRIDMESLAAHAEGVICLXXXXACLGGEVPQHLLHGREEEARKAALRYKEIFG-GDFYL 182 KPR+D E L H+EG+I L ACL GEVP ++L G E+A++AAL Y IFG G+FYL Sbjct: 115 KPRVDREFLKEHSEGLIALS---ACLAGEVPSYILRGEYEKAKEAALFYDSIFGRGNFYL 171 Query: 183 ELQDHGMPEQKRVNPQLIALAAECGIPLVATNDVHYLAKEDAEVQDVLICIGTGKTVDDE 242 ELQDHG+ EQ++VN +LI L+ E GIPLVATNDVHYL K+DA+ +VL+CI TGKT+DDE Sbjct: 172 ELQDHGILEQQKVNKELIRLSKETGIPLVATNDVHYLEKKDAKAHEVLLCIQTGKTIDDE 231 Query: 243 ERLKIGTDQLFLKSGEQMAALFPHVPEAIANTLNIAEKCNLELTFGEHILPAYSPLPEGM 302 +R+ TD+ +LKS E+M LF EAI NT IA+ CN+E F + LP Y LPEG+ Sbjct: 232 DRMSFPTDEFYLKSPEEMENLFSCCKEAIENTEKIADMCNVEFEFNKTKLPKYD-LPEGV 290 Query: 303 DAAAYLRELCWSGLEARYADTPRWSSPEEKDAAGKRLAYELGVIESMGFSDYFLIVWDFI 362 D+ YLR LC+ GL RY + + +RL YEL VIE MG+ DYFLIVWDFI Sbjct: 291 DSCEYLRNLCYEGLYKRYKNPSK--------EVIERLDYELSVIEQMGYVDYFLIVWDFI 342 Query: 363 AFCHRQGIVTGPGRGSSAGSLTAYCLRITDVDPLKYNLLFERFLNPERITMPDIDIDFSD 422 F GI+TGPGRGS+AGSL AY L IT++DP+KYNLLFERFLNPER++MPDID DF Sbjct: 343 KFAKDNGIMTGPGRGSAAGSLVAYTLGITNIDPIKYNLLFERFLNPERVSMPDIDSDFCY 402 Query: 423 ERRDEVIAYVVDKYGKQHVAQIITFGTM 450 ERR EVI YVV KYGK +VAQIITFGTM Sbjct: 403 ERRQEVIDYVVKKYGKDNVAQIITFGTM 430 >B0K7U5.1 B0K7U5_THEP3 DNA polymerase III, alpha subunit EC=2.7.7.7 Length = 1145 Score = 527 bits (1358), Expect = e-148, Method: Compositional matrix adjust. Identities = 262/448 (58%), Positives = 321/448 (71%), Gaps = 20/448 (4%) Query: 4 FVHLHVHSEYSLLDGAARITDLVRRAGEYGMTTLALTDHGVMYGAIPFYKACKASGIKPI 63 FVHLHVHSEYSLLDG+ RI DL+ + E M +A+TDHGVMYG I FYK A GIKPI Sbjct: 2 FVHLHVHSEYSLLDGSCRIKDLIAKTKELNMKAIAITDHGVMYGVIDFYKEAVAQGIKPI 61 Query: 64 IGWEAXXXGCEAYLTAGSRRERGSRKDQPIYHLILLAKNETGYRNLMRLVTIGHLEGQHY 123 IG CE Y+ S ++R D YHL+LLAK+ TGY+NLM++VT+ +EG +Y Sbjct: 62 IG-------CEIYVAPRSMQDREYGIDNENYHLVLLAKDMTGYKNLMKIVTVASIEGFYY 114 Query: 124 KPRIDMESLAAHAEGVICLXXXXACLGGEVPQHLLHGREEEARKAALRYKEIFG-GDFYL 182 KPR+D E L H+EG+I L ACL GEVP ++L G E+A++AAL Y IFG G+FYL Sbjct: 115 KPRVDREFLKEHSEGLIALS---ACLAGEVPSYILRGEYEKAKEAALFYDSIFGRGNFYL 171 Query: 183 ELQDHGMPEQKRVNPQLIALAAECGIPLVATNDVHYLAKEDAEVQDVLICIGTGKTVDDE 242 ELQDHG+ EQ++VN +LI L+ E GIPLVATNDVHYL K+DA+ +VL+CI TGKT+DDE Sbjct: 172 ELQDHGILEQQKVNKELIRLSKETGIPLVATNDVHYLEKKDAKAHEVLLCIQTGKTIDDE 231 Query: 243 ERLKIGTDQLFLKSGEQMAALFPHVPEAIANTLNIAEKCNLELTFGEHILPAYSPLPEGM 302 +R+ TD+ +LKS E+M LF EAI NT IA+ CN+E F + LP Y LPEG+ Sbjct: 232 DRMSFPTDEFYLKSPEEMENLFSCCKEAIENTEKIADMCNVEFEFNKTKLPKYD-LPEGV 290 Query: 303 DAAAYLRELCWSGLEARYADTPRWSSPEEKDAAGKRLAYELGVIESMGFSDYFLIVWDFI 362 D+ YLR LC+ GL RY + + +RL YEL VI+ MG+ DYFLIVWDFI Sbjct: 291 DSCEYLRNLCYEGLYKRYKNPGK--------EVIERLDYELSVIKQMGYVDYFLIVWDFI 342 Query: 363 AFCHRQGIVTGPGRGSSAGSLTAYCLRITDVDPLKYNLLFERFLNPERITMPDIDIDFSD 422 F GI+TGPGRGS+AGSL AY L IT++DP+KYNLLFERFLNPER++MPDID DF Sbjct: 343 KFAKDNGIMTGPGRGSAAGSLVAYTLGITNIDPIKYNLLFERFLNPERVSMPDIDSDFCY 402 Query: 423 ERRDEVIAYVVDKYGKQHVAQIITFGTM 450 ERR EVI YVV KYGK +VAQIITFGTM Sbjct: 403 ERRQEVIDYVVKKYGKDNVAQIITFGTM 430 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,796,326,643 Number of extensions: 122041169 Number of successful extensions: 277269 Number of sequences better than 1.0e-05: 2534 Number of HSP's gapped: 268589 Number of HSP's successfully gapped: 2729 Length of query: 465 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 326 Effective length of database: 1,751,175,118 Effective search space: 570883088468 Effective search space used: 570883088468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)