BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0035.p (430 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDN1.1 B3KDN1_9BACI O-acetylhomoserine/O-acetylserine sulfhydr... 748 0.0 B1DFF2.1 B1DFF2_9BACL O-acetylhomoserine/O-acetylserine sulfhydr... 693 0.0 Q9K9P2.1 Q9K9P2_BACHD O-acetylhomoserine sulfhydrylase 661 0.0 A8FBC1.1 A8FBC1_BACP2 O-acetylhomoserine aminocarboxypropyltrans... 649 0.0 B4AFY0.1 B4AFY0_BACPU O-acetylhomoserine (Thiol)-lyase (O-acetyl... 648 0.0 >B3KDN1.1 B3KDN1_9BACI O-acetylhomoserine/O-acetylserine sulfhydrylase EC=2.5.1.49 Length = 430 Score = 748 bits (1931), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/430 (83%), Positives = 394/430 (91%) Query: 1 LSEDRKLSFETLAVHAGQEIDPTTFARAVPLYQTTSYGFRDAEHAANLFGLKEFGNIYTR 60 +SE+R+ S ETLAVHAGQE+DPTT +RAVPLYQTTSYGF+D+EHAANLF LKEFGNIYTR Sbjct: 1 MSEERQFSIETLAVHAGQELDPTTLSRAVPLYQTTSYGFKDSEHAANLFALKEFGNIYTR 60 Query: 61 LMNPTTDVFEQRXXXXXXXXXXXXXXXXXXXISFSVLNIAGAGDEIVSSASLYGGTYNLF 120 +MNPTTDVFE+R I+FS+LNIAGAGDEIVSS+SLYGGTYNLF Sbjct: 61 IMNPTTDVFEKRIAALEGGAGALATSSGQAAITFSILNIAGAGDEIVSSSSLYGGTYNLF 120 Query: 121 STTLPKLGVKVHFVDSDNPENFRSAITDKTKAIYAETIGNPQGNVLDIEAVAAIAHEHGI 180 S TL KLG+KVHFVDS +PENFR AI KTKA++AETIGNPQGNVLD+EAVAAIAHE+GI Sbjct: 121 SNTLAKLGIKVHFVDSTDPENFRQAINGKTKALFAETIGNPQGNVLDVEAVAAIAHENGI 180 Query: 181 PLIVDNTFPSPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFDWKASGKFPGL 240 PLIVDNTFPSPYLLRPIE+GADIVVHSATKFIGGHGTSIGGVIVDSGKFDW A+ KFPGL Sbjct: 181 PLIVDNTFPSPYLLRPIEYGADIVVHSATKFIGGHGTSIGGVIVDSGKFDWTANDKFPGL 240 Query: 241 TEPDPSYHGLVYTEAVGPIAYIIKARVQLLRDLGASISPFNSWLLLQGLETLHLRLERHS 300 T+PDPSYHG+VYTEAVGPIAYIIKARVQLLRD+GAS+SPFNSWLLLQGLETLHLR+ERHS Sbjct: 241 TQPDPSYHGVVYTEAVGPIAYIIKARVQLLRDIGASLSPFNSWLLLQGLETLHLRVERHS 300 Query: 301 QNALKVAQYLEQHEAVEWVSYAGLPSHPSHKLAQKYLPKGQGAILTFGIKGGAANGVKLI 360 +NALKVA+YLE HEAVEWVSYAGLPSHPS++LAQKYLPKGQGAILTFGIKGGAA G K+I Sbjct: 301 ENALKVAKYLENHEAVEWVSYAGLPSHPSYELAQKYLPKGQGAILTFGIKGGAAAGQKVI 360 Query: 361 ENVKLFSHLANVGDSKSLIIHPASTTHQQLSDAEQITAGVTPELIRLSIGTEAIDDILYD 420 ENVKLFSHLANVGDSKSLIIHPASTTHQQLS+ EQ+TAGV PEL+RLSIGTE+IDDILYD Sbjct: 361 ENVKLFSHLANVGDSKSLIIHPASTTHQQLSEDEQLTAGVNPELLRLSIGTESIDDILYD 420 Query: 421 LEQAIAASQQ 430 LEQAIAASQ+ Sbjct: 421 LEQAIAASQK 430 >B1DFF2.1 B1DFF2_9BACL O-acetylhomoserine/O-acetylserine sulfhydrylase EC=2.5.1.49 Length = 433 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/427 (78%), Positives = 373/427 (87%) Query: 3 EDRKLSFETLAVHAGQEIDPTTFARAVPLYQTTSYGFRDAEHAANLFGLKEFGNIYTRLM 62 E K S +T+AVH GQEIDPTTF+RAVP+YQTTSYGFRD +HAANLF L+EFGNIYTRLM Sbjct: 5 EQNKRSLDTVAVHGGQEIDPTTFSRAVPIYQTTSYGFRDTDHAANLFSLQEFGNIYTRLM 64 Query: 63 NPTTDVFEQRXXXXXXXXXXXXXXXXXXXISFSVLNIAGAGDEIVSSASLYGGTYNLFST 122 NPTTDVFE+R I++S++NIA AGDEIVS+ SLYGGTYNLFST Sbjct: 65 NPTTDVFEKRVAELEGAPAALAVASGQAAITYSIMNIAEAGDEIVSATSLYGGTYNLFST 124 Query: 123 TLPKLGVKVHFVDSDNPENFRSAITDKTKAIYAETIGNPQGNVLDIEAVAAIAHEHGIPL 182 TLPKLG++V FVD NPENFR+AIT++TKA+YAET+GNP+G++LDIEAVAAIAHE+GIPL Sbjct: 125 TLPKLGIQVKFVDPSNPENFRAAITERTKALYAETVGNPKGDILDIEAVAAIAHENGIPL 184 Query: 183 IVDNTFPSPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFDWKASGKFPGLTE 242 IVDNTFPSPYLLRPIE GADIVVHSATKFIGGHGTSIGGVIVD G FDWKASGKFPGLT Sbjct: 185 IVDNTFPSPYLLRPIEHGADIVVHSATKFIGGHGTSIGGVIVDGGNFDWKASGKFPGLTT 244 Query: 243 PDPSYHGLVYTEAVGPIAYIIKARVQLLRDLGASISPFNSWLLLQGLETLHLRLERHSQN 302 PDPSYHG+VYTEAVGPIAYIIKARVQLLRD+GA++SPFNS+LLLQGLETLHLR+ERHS N Sbjct: 245 PDPSYHGVVYTEAVGPIAYIIKARVQLLRDMGAALSPFNSFLLLQGLETLHLRMERHSSN 304 Query: 303 ALKVAQYLEQHEAVEWVSYAGLPSHPSHKLAQKYLPKGQGAILTFGIKGGAANGVKLIEN 362 ALKVA++LE HEAVEWVSY GL SHPS++LA+KYLP GQGAILTFGIKGG G K+I + Sbjct: 305 ALKVAEFLESHEAVEWVSYPGLESHPSYELAKKYLPDGQGAILTFGIKGGVDAGKKVINS 364 Query: 363 VKLFSHLANVGDSKSLIIHPASTTHQQLSDAEQITAGVTPELIRLSIGTEAIDDILYDLE 422 VKLFSHLANVGDSKSLIIHPASTTHQQLS AEQ AGVT LIRLSIGTE I DIL DL+ Sbjct: 365 VKLFSHLANVGDSKSLIIHPASTTHQQLSAAEQEAAGVTAGLIRLSIGTEGIKDILADLD 424 Query: 423 QAIAASQ 429 QA+ ASQ Sbjct: 425 QALRASQ 431 >Q9K9P2.1 Q9K9P2_BACHD O-acetylhomoserine sulfhydrylase Length = 430 Score = 661 bits (1706), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/429 (73%), Positives = 370/429 (86%) Query: 1 LSEDRKLSFETLAVHAGQEIDPTTFARAVPLYQTTSYGFRDAEHAANLFGLKEFGNIYTR 60 ++ + + ET AVH+GQEIDPTT +RAVPLYQTTSYGF+D +HAANLF L EFGNIYTR Sbjct: 1 MNHENQWQLETKAVHSGQEIDPTTLSRAVPLYQTTSYGFKDTDHAANLFSLSEFGNIYTR 60 Query: 61 LMNPTTDVFEQRXXXXXXXXXXXXXXXXXXXISFSVLNIAGAGDEIVSSASLYGGTYNLF 120 LMNPTTDVFE+R I++S+LNIA AGDEIVS++SLYGGTYNLF Sbjct: 61 LMNPTTDVFEKRVAALEGGAAALATASGQAAITYSILNIAEAGDEIVSASSLYGGTYNLF 120 Query: 121 STTLPKLGVKVHFVDSDNPENFRSAITDKTKAIYAETIGNPQGNVLDIEAVAAIAHEHGI 180 S TLPKLGV V FVD +PENF++AIT+KTKAI+AE+IGNP+G+VLDIEAVA +AH+H + Sbjct: 121 SITLPKLGVNVRFVDPSDPENFKAAITEKTKAIFAESIGNPKGDVLDIEAVAKVAHDHHL 180 Query: 181 PLIVDNTFPSPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFDWKASGKFPGL 240 PLIVDNTFPSPYLL+PI+ GADIVVHSATKFIGGHGTSIGG+IVD G FDW + ++PGL Sbjct: 181 PLIVDNTFPSPYLLQPIKHGADIVVHSATKFIGGHGTSIGGIIVDGGTFDWAKTDRYPGL 240 Query: 241 TEPDPSYHGLVYTEAVGPIAYIIKARVQLLRDLGASISPFNSWLLLQGLETLHLRLERHS 300 T PDPSYHG+VYT+AVGPIAYIIKARVQLLRD+GA+ISPFNS+LLLQGLETLHLR+ERHS Sbjct: 241 TTPDPSYHGVVYTDAVGPIAYIIKARVQLLRDMGAAISPFNSFLLLQGLETLHLRMERHS 300 Query: 301 QNALKVAQYLEQHEAVEWVSYAGLPSHPSHKLAQKYLPKGQGAILTFGIKGGAANGVKLI 360 +NA KVA++LEQH+AVE VSY+GLPSHPS+ LA+KYLPKGQGAILTF +KGG G KLI Sbjct: 301 ENAYKVAEFLEQHQAVESVSYSGLPSHPSYPLAKKYLPKGQGAILTFEVKGGVEAGKKLI 360 Query: 361 ENVKLFSHLANVGDSKSLIIHPASTTHQQLSDAEQITAGVTPELIRLSIGTEAIDDILYD 420 +V+LFSHLANVGDSKSLIIHPASTTHQQLS+AEQ AGVTP +IRLS+GTE+I DI+ D Sbjct: 361 HSVQLFSHLANVGDSKSLIIHPASTTHQQLSEAEQRDAGVTPGMIRLSVGTESIHDIITD 420 Query: 421 LEQAIAASQ 429 L+QAI ASQ Sbjct: 421 LKQAIEASQ 429 >A8FBC1.1 A8FBC1_BACP2 O-acetylhomoserine aminocarboxypropyltransferase EC=2.5.1.49 Length = 431 Score = 649 bits (1674), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/429 (73%), Positives = 360/429 (83%) Query: 1 LSEDRKLSFETLAVHAGQEIDPTTFARAVPLYQTTSYGFRDAEHAANLFGLKEFGNIYTR 60 +SE+R ET A+HAGQE+D T++RAVP+YQT+S+GFRD+EHAANLFGL+E GNIY+R Sbjct: 1 MSEERPFRLETKAIHAGQELDSATYSRAVPIYQTSSFGFRDSEHAANLFGLQEPGNIYSR 60 Query: 61 LMNPTTDVFEQRXXXXXXXXXXXXXXXXXXXISFSVLNIAGAGDEIVSSASLYGGTYNLF 120 +MNPT DVFE+R ++S+LNIAGAGDEIVSS+SLYGGTYNLF Sbjct: 61 IMNPTNDVFEKRIAELEGGIGALAVSSGQAATTYSILNIAGAGDEIVSSSSLYGGTYNLF 120 Query: 121 STTLPKLGVKVHFVDSDNPENFRSAITDKTKAIYAETIGNPQGNVLDIEAVAAIAHEHGI 180 + TL KLG+ V FVDS N E +AITDKTKA+YAE+IGNP+G++L IEA++ IAH+H I Sbjct: 121 AHTLKKLGITVKFVDSSNLEELEAAITDKTKAVYAESIGNPKGDILHIEAISTIAHKHHI 180 Query: 181 PLIVDNTFPSPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFDWKASGKFPGL 240 PLIVDNT SPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKF W S KF GL Sbjct: 181 PLIVDNTLASPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFPWADSDKFKGL 240 Query: 241 TEPDPSYHGLVYTEAVGPIAYIIKARVQLLRDLGASISPFNSWLLLQGLETLHLRLERHS 300 TEPDPSYHGL YTEA+G AYI KARVQLLRDLGA++SPFNS+LLLQGLETLHLRLERHS Sbjct: 241 TEPDPSYHGLTYTEAIGEAAYITKARVQLLRDLGAALSPFNSFLLLQGLETLHLRLERHS 300 Query: 301 QNALKVAQYLEQHEAVEWVSYAGLPSHPSHKLAQKYLPKGQGAILTFGIKGGAANGVKLI 360 +NALK AQ+L+ H V+WVSY GL SH S++LAQ YLPKGQGAILTFGIKGG G KLI Sbjct: 301 ENALKTAQFLQDHPLVDWVSYPGLESHESYELAQTYLPKGQGAILTFGIKGGREAGKKLI 360 Query: 361 ENVKLFSHLANVGDSKSLIIHPASTTHQQLSDAEQITAGVTPELIRLSIGTEAIDDILYD 420 ++VKLFSHLANVGDSKSLIIHPASTTHQQLS+AEQI GVTPELIRLS+GTEAI+DI+ D Sbjct: 361 DSVKLFSHLANVGDSKSLIIHPASTTHQQLSEAEQIATGVTPELIRLSVGTEAIEDIIAD 420 Query: 421 LEQAIAASQ 429 LEQAI SQ Sbjct: 421 LEQAITKSQ 429 >B4AFY0.1 B4AFY0_BACPU O-acetylhomoserine (Thiol)-lyase (O-acetylhomoserinesulfhydrylase) (OAH sulfhydrylase) (Homocysteine synthase) EC=2.5.1.49 Length = 431 Score = 648 bits (1671), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/429 (73%), Positives = 360/429 (83%) Query: 1 LSEDRKLSFETLAVHAGQEIDPTTFARAVPLYQTTSYGFRDAEHAANLFGLKEFGNIYTR 60 +SE+R ET A+HAGQE+D T++RAVP+YQT+S+GFRD+EHAANLFGL+E GNIY+R Sbjct: 1 MSEERPFRLETKAIHAGQELDSATYSRAVPIYQTSSFGFRDSEHAANLFGLQEPGNIYSR 60 Query: 61 LMNPTTDVFEQRXXXXXXXXXXXXXXXXXXXISFSVLNIAGAGDEIVSSASLYGGTYNLF 120 +MNPT DVFE+R ++S+LNIAGAGDEIVSS+SLYGGTYNLF Sbjct: 61 IMNPTNDVFEKRIAELEGGIGALAVSSGQAATTYSILNIAGAGDEIVSSSSLYGGTYNLF 120 Query: 121 STTLPKLGVKVHFVDSDNPENFRSAITDKTKAIYAETIGNPQGNVLDIEAVAAIAHEHGI 180 + TL KLG+ V FVDS N E +AITDKTKA+YAE+IGNP+G++L IEA++AIAH+H I Sbjct: 121 AHTLKKLGITVKFVDSSNLEELEAAITDKTKAVYAESIGNPKGDILHIEAISAIAHKHHI 180 Query: 181 PLIVDNTFPSPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFDWKASGKFPGL 240 PLIVDNT SPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKF W S KF GL Sbjct: 181 PLIVDNTLASPYLLRPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFPWADSDKFKGL 240 Query: 241 TEPDPSYHGLVYTEAVGPIAYIIKARVQLLRDLGASISPFNSWLLLQGLETLHLRLERHS 300 TEPDPSYHGL YTEA+G AYI KARVQLLRDLGA++SPFNS+LLLQGLETLHLRLERHS Sbjct: 241 TEPDPSYHGLTYTEAIGEAAYITKARVQLLRDLGAALSPFNSFLLLQGLETLHLRLERHS 300 Query: 301 QNALKVAQYLEQHEAVEWVSYAGLPSHPSHKLAQKYLPKGQGAILTFGIKGGAANGVKLI 360 +NALK AQ+L+ H V+WVSY GL SH S++LAQ YLPKGQGAILTFGIKGG G KLI Sbjct: 301 ENALKTAQFLQDHPLVDWVSYPGLESHESYELAQTYLPKGQGAILTFGIKGGREAGKKLI 360 Query: 361 ENVKLFSHLANVGDSKSLIIHPASTTHQQLSDAEQITAGVTPELIRLSIGTEAIDDILYD 420 ++VKLFSHLANVGDSKSLIIHPASTTHQQLS+AEQ GVTPELIRLS+GTEAI+DI+ D Sbjct: 361 DSVKLFSHLANVGDSKSLIIHPASTTHQQLSEAEQKATGVTPELIRLSVGTEAIEDIIAD 420 Query: 421 LEQAIAASQ 429 LEQAI SQ Sbjct: 421 LEQAIKKSQ 429 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,518,505,019 Number of extensions: 105769044 Number of successful extensions: 270011 Number of sequences better than 1.0e-05: 4437 Number of HSP's gapped: 260832 Number of HSP's successfully gapped: 6765 Length of query: 430 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 291 Effective length of database: 1,751,175,118 Effective search space: 509591959338 Effective search space used: 509591959338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)