BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0014.p (141 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8ET97.1 Q8ET97_OCEIH Sulfate permease 161 2e-38 B7GG39.1 B7GG39_ANOFW Sulfate permease 160 3e-38 A6CPA8.1 A6CPA8_9BACI Sulfate permease 159 8e-38 A8W074.1 A8W074_9BACI PAS sensor protein 156 7e-37 B3K9V6.1 B3K9V6_9BACI Sulphate transporter 154 3e-36 >Q8ET97.1 Q8ET97_OCEIH Sulfate permease Length = 483 Score = 161 bits (407), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 79/141 (56%), Positives = 101/141 (71%) Query: 1 VLAGILQILMGMLKRGRFITFLPQPVMTGFVNALAILIFMAQLTHFSGKGWVMYALVVLT 60 +L G+LQIL G+ K RF+ F+P+ VMTGFVNALAILIF +QL HF G+ W+MYALV LT Sbjct: 94 ILTGVLQILFGIFKLARFMKFVPRTVMTGFVNALAILIFTSQLQHFVGETWMMYALVALT 153 Query: 61 LLIIYSVPRFTKAVPSAXXXXXXXXXXXXXXHLDVRTVGDMGDITPALPVFHLPQLPFTL 120 L IIY PR TKAVPS ++ VR VGDMG++T LP+F LP +P L Sbjct: 154 LAIIYIFPRITKAVPSTLVAIIVVTIMVLTMNIGVRNVGDMGELTQTLPMFALPNIPLNL 213 Query: 121 DTLLIIAPYSLSLAVVGLLES 141 +TL+II PY+L+L++VG+LES Sbjct: 214 ETLMIIFPYALALSIVGILES 234 >B7GG39.1 B7GG39_ANOFW Sulfate permease Length = 489 Score = 160 bits (405), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 81/141 (57%), Positives = 100/141 (70%) Query: 1 VLAGILQILMGMLKRGRFITFLPQPVMTGFVNALAILIFMAQLTHFSGKGWVMYALVVLT 60 ++ GI+QI +G+L+ G + F+P+PVM GFVNALAILIF AQL HF G+ WVMYALV L Sbjct: 94 IVTGIIQIGLGILQVGNLLKFIPRPVMVGFVNALAILIFAAQLPHFQGESWVMYALVGLG 153 Query: 61 LLIIYSVPRFTKAVPSAXXXXXXXXXXXXXXHLDVRTVGDMGDITPALPVFHLPQLPFTL 120 L IIY PR T A+PS LDVRTVGDMG+I+ LP F +P +P TL Sbjct: 154 LFIIYVFPRITTAIPSPLVAIVVITTIAFVAKLDVRTVGDMGEISATLPSFFIPNVPLTL 213 Query: 121 DTLLIIAPYSLSLAVVGLLES 141 +TL+II PYSLSLA+VGL+ES Sbjct: 214 ETLMIIFPYSLSLALVGLIES 234 >A6CPA8.1 A6CPA8_9BACI Sulfate permease Length = 482 Score = 159 bits (402), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 82/141 (58%), Positives = 98/141 (69%) Query: 1 VLAGILQILMGMLKRGRFITFLPQPVMTGFVNALAILIFMAQLTHFSGKGWVMYALVVLT 60 +L GILQIL G+ K R++ F+P+ VM GFVNALAILIF +QL HF G+ W+MYALV LT Sbjct: 95 ILTGILQILFGVFKLARYMKFIPRSVMVGFVNALAILIFSSQLQHFHGEEWIMYALVGLT 154 Query: 61 LLIIYSVPRFTKAVPSAXXXXXXXXXXXXXXHLDVRTVGDMGDITPALPVFHLPQLPFTL 120 L IIY +P FTK VPS + VRTVGDMG I+ LP F LP +P TL Sbjct: 155 LFIIYLLPYFTKVVPSTLVAIVTVTILSITMNYGVRTVGDMGTISQTLPQFFLPDIPLTL 214 Query: 121 DTLLIIAPYSLSLAVVGLLES 141 +TL+II PYS +LAVVGLLES Sbjct: 215 ETLMIIFPYSAALAVVGLLES 235 >A8W074.1 A8W074_9BACI PAS sensor protein Length = 487 Score = 156 bits (394), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 77/141 (54%), Positives = 98/141 (69%) Query: 1 VLAGILQILMGMLKRGRFITFLPQPVMTGFVNALAILIFMAQLTHFSGKGWVMYALVVLT 60 +L GI+QIL G+ K + F+P+ VM GFVNALAILIF +QL HF G+ W+MYALV LT Sbjct: 96 ILTGIIQILFGVFKLSSIMKFVPRSVMIGFVNALAILIFTSQLQHFVGETWIMYALVALT 155 Query: 61 LLIIYSVPRFTKAVPSAXXXXXXXXXXXXXXHLDVRTVGDMGDITPALPVFHLPQLPFTL 120 L IIY +PRFT AVPS ++ VRTVGDMG ++ LP+F LP +P T Sbjct: 156 LAIIYILPRFTTAVPSTLVAIIVVTAIAIVMNMGVRTVGDMGSLSSTLPIFALPNIPLTF 215 Query: 121 DTLLIIAPYSLSLAVVGLLES 141 +T +II PYSL+LA+VG+LES Sbjct: 216 ETFMIILPYSLALAIVGILES 236 >B3K9V6.1 B3K9V6_9BACI Sulphate transporter Length = 483 Score = 154 bits (388), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 81/141 (57%), Positives = 101/141 (71%) Query: 1 VLAGILQILMGMLKRGRFITFLPQPVMTGFVNALAILIFMAQLTHFSGKGWVMYALVVLT 60 +L GILQ LMG LK GRFITF+PQPV+TGFVN+LAI+IF+AQL +F G+ W MY +V+ T Sbjct: 94 ILTGILQWLMGALKFGRFITFVPQPVITGFVNSLAIIIFLAQLPNFKGESWPMYLMVIGT 153 Query: 61 LLIIYSVPRFTKAVPSAXXXXXXXXXXXXXXHLDVRTVGDMGDITPALPVFHLPQLPFTL 120 LLIIY +P TKAVPSA V+TVGDMG+IT ALP FH+P + +L Sbjct: 154 LLIIYLLPLVTKAVPSALVAIIVMTIVAVWFKAPVQTVGDMGNITQALPFFHIPNVTVSL 213 Query: 121 DTLLIIAPYSLSLAVVGLLES 141 D ++ I P SL+LAVVG+ ES Sbjct: 214 DMIMTILPISLALAVVGMTES 234 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.146 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 707,218,130 Number of extensions: 23236795 Number of successful extensions: 81431 Number of sequences better than 1.0e-05: 868 Number of HSP's gapped: 80454 Number of HSP's successfully gapped: 869 Length of query: 141 Length of database: 3,060,696,203 Length adjustment: 104 Effective length of query: 37 Effective length of database: 2,080,910,643 Effective search space: 76993693791 Effective search space used: 76993693791 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 126 (53.1 bits)