BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0007.p (427 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZC89.1 C0ZC89_BREBN Conserved hypothetical membrane protein 514 e-144 C4V572.1 C4V572_9FIRM Citrate transporter 489 e-136 B7AXZ1.1 B7AXZ1_9FIRM Putative uncharacterized protein 471 e-131 C1TI54.1 C1TI54_9FIRM Na+/H+ antiporter NhaD-like permease 447 e-124 A5D532.1 A5D532_PELTS Na+/H+ antiporter NhaD and related arsenit... 438 e-121 >C0ZC89.1 C0ZC89_BREBN Conserved hypothetical membrane protein Length = 427 Score = 514 bits (1324), Expect = e-144, Method: Compositional matrix adjust. Identities = 257/427 (60%), Positives = 321/427 (75%), Gaps = 1/427 (0%) Query: 1 MEQQGIWAIGIFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXVSQETALEHIDFNTXXXX 60 M Q ++AIGIFL+TY IISEK+HRT+ ++QE A+ HIDFNT Sbjct: 1 MTNQALFAIGIFLVTYAFIISEKLHRTIVAMSGGILMVLFGIITQEQAIHHIDFNTLGLL 60 Query: 61 XXXXXXXXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVP 120 ITA+TG+F YVA++AAK+AKG+P RI FLDNVTTVLL+VP Sbjct: 61 IGMMILVAITAQTGVFKYVAIRAAKVAKGKPIRILVYLSLITAIASAFLDNVTTVLLIVP 120 Query: 121 VTFSITRQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPV 180 VTFSI RQL+++P+PFL+S+IIASN GGTATLIGDPPNIMIGS+V EL FM F+ NL+P+ Sbjct: 121 VTFSIARQLQLNPIPFLISEIIASNAGGTATLIGDPPNIMIGSSVPELDFMKFLVNLSPI 180 Query: 181 ILIIILAYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFF 240 I++I+ + +L++ KQL TSPEL +M+++ER ITD +LL++ L V+ LTILGF Sbjct: 181 IIVIMAVTVVCLVLIYRKQLVTSPELSAKIMQLNERDEITDSRLLKKSLTVMALTILGFM 240 Query: 241 LHQAIHLESATVXXXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETGV 300 LH A+HLESAT+ E L A + VEW TIFFFIGLFVLVSGLVETGV Sbjct: 241 LHGALHLESATI-ALTGAFLLLLLTGEHYLEDAISKVEWNTIFFFIGLFVLVSGLVETGV 299 Query: 301 IAELAGQAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNLGIHNLEP 360 IA+LA +AM+LTGG+ +S+LILWLSAIASAF+DNIPFVATMIP+I+EMG+LGI NLEP Sbjct: 300 IAKLANEAMKLTGGDSLKTSLLILWLSAIASAFVDNIPFVATMIPMIKEMGSLGITNLEP 359 Query: 361 LWWSLALGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLY 420 LWWSLALGACLGGNGTLIGASAN+IVAGLAAKEG+ I F ++K+ FPLM+LS++I+ +Y Sbjct: 360 LWWSLALGACLGGNGTLIGASANVIVAGLAAKEGHHIGFFSFMKIAFPLMILSILIAHVY 419 Query: 421 IYLRYLI 427 +YLRY I Sbjct: 420 VYLRYFI 426 >C4V572.1 C4V572_9FIRM Citrate transporter Length = 425 Score = 489 bits (1260), Expect = e-136, Method: Compositional matrix adjust. Identities = 242/418 (57%), Positives = 307/418 (73%), Gaps = 1/418 (0%) Query: 11 IFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXVSQETALEHIDFNTXXXXXXXXXXXXIT 70 IF++ Y +I+SEK+HRTV +SQE AL H+DF T +T Sbjct: 8 IFVVMYMVIVSEKIHRTVAAMIGAVLMMLLGILSQEVALHHVDFETLGLLVGMMVLVGVT 67 Query: 71 AETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVPVTFSITRQLR 130 ETGLF YVA+KAAK A+ EP+RI FLDNVTTVLLMVPVTFSIT+ L+ Sbjct: 68 KETGLFDYVAIKAAKSAQAEPRRILVYLCVITAVFSAFLDNVTTVLLMVPVTFSITKILK 127 Query: 131 IHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPVILIIILAYLP 190 + P+P+LL+QIIASN+GGTATLIGDPPNIMIGSAVKELTF+ FI +L P+ ++ ++ L Sbjct: 128 LDPMPYLLTQIIASNIGGTATLIGDPPNIMIGSAVKELTFVMFIEHLAPIAIVCMVVVLF 187 Query: 191 LFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFFLHQAIHLESA 250 + ++ KQL T+PELQ +M+MDE+A ITD LL+R L+VLGLTILGFF H H+ES+ Sbjct: 188 IMSTIYRKQLVTTPELQAELMQMDEKAAITDHALLKRALFVLGLTILGFFTHSFTHIESS 247 Query: 251 TVX-XXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETGVIAELAGQAM 309 + E ++ KA +VEW TIFFFIGLF+ V GL+E G+IA+LA A+ Sbjct: 248 LIALTGGFLLLLLAGGSEHLVEKAMHAVEWPTIFFFIGLFIAVGGLIEVGIIAQLAETAV 307 Query: 310 ELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNLGIHNLEPLWWSLALGA 369 TGG+L A+S+LILW+SAI S+ LDNIPFVATMIPLIQ MG +GI NLEPLWWSLALGA Sbjct: 308 NATGGDLTATSLLILWMSAIISSVLDNIPFVATMIPLIQNMGAMGIANLEPLWWSLALGA 367 Query: 370 CLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLYIYLRYLI 427 CLGGNGTL+GASANLIVAG+AA+ G I+F+RY K+GFPLMLL++V+S++Y+Y+RYL+ Sbjct: 368 CLGGNGTLVGASANLIVAGMAAERGVHISFVRYFKIGFPLMLLTIVLSTVYVYVRYLL 425 >B7AXZ1.1 B7AXZ1_9FIRM Putative uncharacterized protein Length = 425 Score = 471 bits (1213), Expect = e-131, Method: Compositional matrix adjust. Identities = 236/418 (56%), Positives = 299/418 (71%), Gaps = 1/418 (0%) Query: 11 IFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXVSQETALEHIDFNTXXXXXXXXXXXXIT 70 IF+L Y LI+SEKVHRT+ + +TAL HIDFNT IT Sbjct: 8 IFVLMYVLIVSEKVHRTIVAMMGAILMIIVGIMPVDTALHHIDFNTLGLLIGMMILVSIT 67 Query: 71 AETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVPVTFSITRQLR 130 A+TGLF YVA+K AK+AK +P+R+ FLDNVTTVLLMVPVTF+IT +L Sbjct: 68 AKTGLFDYVAIKTAKVAKAQPRRLLIYLGFITALFSAFLDNVTTVLLMVPVTFAITNKLH 127 Query: 131 IHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPVILIIILAYLP 190 I +PFLL+QI+ASNVGGTATLIGDPPNIMIGSAVKELTF++FI NLTPV+++ + A L Sbjct: 128 IPVVPFLLTQILASNVGGTATLIGDPPNIMIGSAVKELTFVAFIENLTPVVILCMAAVLF 187 Query: 191 LFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFFLHQAIHLESA 250 + +++ KQ+ T PELQ +M+MDE+ + DRKLL R L+VLGLTILGFF H H+ES+ Sbjct: 188 VMDVLYRKQMVTKPELQAEIMQMDEKKSLKDRKLLARSLFVLGLTILGFFTHSFTHIESS 247 Query: 251 TVXXXXXXXXXXXXXXEKM-LHKAFASVEWVTIFFFIGLFVLVSGLVETGVIAELAGQAM 309 V + + + VEW TIFFFIGLFV V GL ETGVI +LA A+ Sbjct: 248 VVALFGAFLLLLLAGGSQHDVDFSMRKVEWATIFFFIGLFVAVGGLSETGVIRDLATGAV 307 Query: 310 ELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNLGIHNLEPLWWSLALGA 369 LT G++ +S+L+LWLSA+ S+ LDNIPFVATMIPLIQ MG +G+ NLEP+WWSL+LGA Sbjct: 308 ALTEGDVTKTSLLVLWLSALVSSVLDNIPFVATMIPLIQNMGAMGVDNLEPVWWSLSLGA 367 Query: 370 CLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLYIYLRYLI 427 CLGGNGTL+GASANLIVAG+AA G I+FM Y K+ FP+M++++ IS++Y+YLRYL+ Sbjct: 368 CLGGNGTLVGASANLIVAGMAADRGVKISFMNYFKISFPIMIMTIAISTVYVYLRYLL 425 >C1TI54.1 C1TI54_9FIRM Na+/H+ antiporter NhaD-like permease Length = 427 Score = 447 bits (1151), Expect = e-124, Method: Compositional matrix adjust. Identities = 235/426 (55%), Positives = 311/426 (73%), Gaps = 1/426 (0%) Query: 1 MEQQGIWAIGIFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXVSQETALEHIDFNTXXXX 60 MEQQ A GIFL+ Y LII+EK++RT+ V+QE+AL HIDFNT Sbjct: 1 MEQQAALAGGIFLIAYALIIAEKINRTIIAMIGGLMMIALGIVTQESALHHIDFNTLGLL 60 Query: 61 XXXXXXXXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVP 120 IT +TG+FSY+A+ AAK AKG+P +I FLDNVTTVLLMVP Sbjct: 61 AGMMIIVAITGKTGIFSYIAIAAAKKAKGDPIKILIYLGLITAVFSAFLDNVTTVLLMVP 120 Query: 121 VTFSITRQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPV 180 +TF+ITR+L+I+P P+L++QI+ASN+GGT+TLIGDPPNIMIGS V+EL+FM FI+NL + Sbjct: 121 ITFTITRKLKINPEPYLITQILASNIGGTSTLIGDPPNIMIGSVVQELSFMDFINNLALI 180 Query: 181 ILIIILAYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFF 240 +I+L + + ++ + K+++T+ EL+ S+M +DE I DRKLL+ C VLGLTILGFF Sbjct: 181 STLILLVTIAILIVFYRKKIRTTDELKASLMILDEIEEIKDRKLLKTCFIVLGLTILGFF 240 Query: 241 LHQAIHLESATVXXX-XXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETG 299 HQ LESAT+ + L AF VEW T+FFFIGLFVLV GL++TG Sbjct: 241 SHQIFDLESATIALSGAFILLLFTSKTDYDLEHAFYKVEWSTLFFFIGLFVLVGGLIDTG 300 Query: 300 VIAELAGQAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNLGIHNLE 359 +I LA A++ TGG+ +S LI+WLSA+ASAF+DNIPFVATMIPLIQ++G+ G+ N+E Sbjct: 301 IIGYLARLAIKFTGGDTLITSTLIIWLSAVASAFIDNIPFVATMIPLIQDIGSRGVSNME 360 Query: 360 PLWWSLALGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSL 419 LWWSL+LGACLGGNG+LIG+SANLIVAGLAA++G I+FMR+L +GFPLM++S++I+++ Sbjct: 361 TLWWSLSLGACLGGNGSLIGSSANLIVAGLAAQQGCKISFMRFLLIGFPLMVISIIIANI 420 Query: 420 YIYLRY 425 YIYLRY Sbjct: 421 YIYLRY 426 >A5D532.1 A5D532_PELTS Na+/H+ antiporter NhaD and related arsenite permeases Length = 427 Score = 438 bits (1127), Expect = e-121, Method: Compositional matrix adjust. Identities = 225/424 (53%), Positives = 295/424 (69%), Gaps = 4/424 (0%) Query: 4 QGIWAIGIFLLTYGLIISEKVHRTVXXXXXXXXXXXXXXVSQETALEHIDFNTXXXXXXX 63 Q +A +FL Y L+I++K+HRT+ +SQE A+ IDFNT Sbjct: 6 QITFASAVFLGIYALLIADKIHRTIVALCGAVIMLLAGIISQERAVRAIDFNTIGLLVGM 65 Query: 64 XXXXXITAETGLFSYVAVKAAKLAKGEPKRIXXXXXXXXXXXXXFLDNVTTVLLMVPVTF 123 IT TG+F Y+AV +AK AKGEP +I FLDNVTTVLL+VPVTF Sbjct: 66 MVIVGITRRTGIFEYLAVISAKWAKGEPVKIMLSLATVTAVLSAFLDNVTTVLLIVPVTF 125 Query: 124 SITRQLRIHPLPFLLSQIIASNVGGTATLIGDPPNIMIGSAVKELTFMSFISNLTPVILI 183 +I RQL+I+P+PFL ++IIASN+GGTATLIGDPPNIMIGSA L FM F+ LTPV+++ Sbjct: 126 AIARQLKINPVPFLFAEIIASNIGGTATLIGDPPNIMIGSATG-LGFMDFVIALTPVVIV 184 Query: 184 IILAYLPLFLLMFGKQLKTSPELQRSVMEMDERAMITDRKLLRRCLWVLGLTILGFFLHQ 243 + +A + +++ KQL T+P+LQ+++M MDE+ I D +L++ LW++GLTILGF LHQ Sbjct: 185 VHIANMIWLRVLYKKQLVTTPDLQKNIMNMDEKEEIKDPVMLKKSLWIIGLTILGFVLHQ 244 Query: 244 AIHLESATVXXXXXXXXXXXXXXEKMLHKAFASVEWVTIFFFIGLFVLVSGLVETGVIAE 303 +HLESAT+ + +A +VEW IFFF GLFV+V L E GVI Sbjct: 245 YLHLESATIALSGAAFLLLVTRDDP--GRALGAVEWPVIFFFAGLFVVVGALEEVGVIEY 302 Query: 304 LAGQAMELTGGNLAASSMLILWLSAIASAFLDNIPFVATMIPLIQEMGNL-GIHNLEPLW 362 +A ++++LTGG + + +LILWLSAIASAF+DNIPFVATMIPLIQ+MG L GI +L+PLW Sbjct: 303 IAKESLKLTGGAMVPTGLLILWLSAIASAFVDNIPFVATMIPLIQDMGRLGGIPDLKPLW 362 Query: 363 WSLALGACLGGNGTLIGASANLIVAGLAAKEGYPITFMRYLKVGFPLMLLSMVISSLYIY 422 WSL+LGACLGGNGTLIGASAN++VAGLA K G +TFM + KV FP+ML+S+ I + Y+ Sbjct: 363 WSLSLGACLGGNGTLIGASANVVVAGLAEKRGVIMTFMSFTKVAFPMMLMSVAICTGYLL 422 Query: 423 LRYL 426 LRYL Sbjct: 423 LRYL 426 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,009,396,007 Number of extensions: 70317182 Number of successful extensions: 277723 Number of sequences better than 1.0e-05: 1325 Number of HSP's gapped: 275723 Number of HSP's successfully gapped: 1515 Length of query: 427 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 288 Effective length of database: 1,751,175,118 Effective search space: 504338433984 Effective search space used: 504338433984 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)