BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0377.0004.p (367 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D7P4.1 B1D7P4_9BACL Luciferase family protein 379 e-103 Q9F9Q6.1 Q9F9Q6_BACAM Putative uncharacterized protein 366 2e-99 A7Z540.1 A7Z540_BACA2 Putative uncharacterized protein 363 2e-98 B5WQI6.1 B5WQI6_9BURK Luciferase-like monooxygenase 320 1e-85 C1XFV0.1 C1XFV0_MEIRU Flavin-dependent oxidoreductase, F420-depe... 315 5e-84 >B1D7P4.1 B1D7P4_9BACL Luciferase family protein Length = 353 Score = 379 bits (973), Expect = e-103, Method: Compositional matrix adjust. Identities = 184/346 (53%), Positives = 240/346 (69%), Gaps = 3/346 (0%) Query: 12 FEFGLYTLGDIVTDCHTGQRISPQQRMKEVIEAAKLADEAGLDVFGVGEHHRLDFVISSV 71 FEFG+YTLGD+ D TG + +RMKE+I AAK+ADEAG++VFGVGEHHRLDF +SS Sbjct: 10 FEFGIYTLGDLAVDSRTGGLKNAGERMKEIIRAAKVADEAGINVFGVGEHHRLDFALSSP 69 Query: 72 PVVLAAISQVTRRIKLTSATTVLSTIDPVRVFEDFATLDLLSGGRAEIISGRGAFLESFP 131 PV+LAAI+Q T RI+LTSATTVL T DPVRVFEDFATLDL+S GRAEII+GRGA++ESFP Sbjct: 70 PVILAAIAQATSRIRLTSATTVLGTADPVRVFEDFATLDLISDGRAEIIAGRGAYIESFP 129 Query: 132 LFGYDLEDYKQLFTXXXXXXXXXXXREVMNWQGNFRSSLKNAEIAPRPLQQKLPLWVXXX 191 LFG++L+DYK+LF E + W G RS L++AEIAPRP Q +P+WV Sbjct: 130 LFGFELDDYKELFAEKLELMRQLNLSERITWNGRLRSPLEDAEIAPRPKQAAIPIWVGVG 189 Query: 192 XXXXXXXXXXXXXVGMAIAILSGSPEPFQNLADTYRRSGAEAGHAASDLKIAITSHGYIA 251 GMA+A+L G P +++L D Y+ +G AG+ +L++AIT H Y+A Sbjct: 190 STAESAALAGRHGAGMALALLGGEPSKYKHLVDCYKEAGVAAGYRKEELRVAITGHCYVA 249 Query: 252 KTSQQALDEYYPYYYSYRNAISPHPGQEYRVSRRDFGRFVSPVNTLAVGSPQQIIEKILY 311 KT+ QALDE+Y Y Y + +SR DF + ++LA+GSP+ I EKI+ Sbjct: 250 KTTGQALDEFYAGYSVYYSQFMGGKENGTPLSREDFEKL---ADSLAIGSPELIAEKIIA 306 Query: 312 QHELFGHNRFMTQLDIGGLPYAKVAAAIELLATEVAPVVRREIAKK 357 QH+LFGH+RFM Q+DIGG+P A+V ++I LLA EVAP+VR + K Sbjct: 307 QHKLFGHSRFMAQMDIGGMPAAQVESSIRLLAKEVAPLVREALQIK 352 >Q9F9Q6.1 Q9F9Q6_BACAM Putative uncharacterized protein Length = 356 Score = 366 bits (939), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 178/346 (51%), Positives = 241/346 (69%), Gaps = 2/346 (0%) Query: 12 FEFGLYTLGDIVTDCHTGQRISPQQRMKEVIEAAKLADEAGLDVFGVGEHHRLDFVISSV 71 E G+Y+ ++ D TG+ +S +QR+ E+I+ AKLAD+AGLDVFGVGEHHRLD+ +SS Sbjct: 9 MEIGIYSFAELYPDPLTGKTVSAKQRLDELIKLAKLADDAGLDVFGVGEHHRLDYAVSSP 68 Query: 72 PVVLAAISQVTRRIKLTSATTVLSTIDPVRVFEDFATLDLLSGGRAEIISGRGAFLESFP 131 PV+L+AISQ+T IKLTSATTVLST DPVR+FEDFATLDL+S GRAEII+GRGAFL+SFP Sbjct: 69 PVILSAISQLTENIKLTSATTVLSTEDPVRLFEDFATLDLISNGRAEIIAGRGAFLDSFP 128 Query: 132 LFGYDLEDYKQLFTXXXXX-XXXXXXREVMNWQGNFRSSLKNAEIAPRPLQQKLPLWVXX 190 LFGYD+ YK+LF EV+ W+G +R L NAEI+P+P +PLW+ Sbjct: 129 LFGYDVGHYKELFEEHIELFLTLNSEEEVITWKGCYRPPLNNAEISPQPFHGSIPLWLGV 188 Query: 191 XXXXXXXXXXXXXXVGMAIAILSGSPEPFQNLADTYRRSGAEAGHAASDLKIAITSHGYI 250 G+A+A+LSG P + L D YR + ++AGH ++K+A+T H I Sbjct: 189 SGTPESAERAGSAGAGLALAVLSGDPRSSKPLVDKYREAASKAGHPPENVKVAVTGH-TI 247 Query: 251 AKTSQQALDEYYPYYYSYRNAISPHPGQEYRVSRRDFGRFVSPVNTLAVGSPQQIIEKIL 310 +KT++QA+ E+YPY+ +Y + + + G +++ DF R L VGS QQIIEKI+ Sbjct: 248 SKTNEQAIQEFYPYHSNYWSRVHSYHGIHEPLTKEDFERAAGKETALFVGSSQQIIEKIM 307 Query: 311 YQHELFGHNRFMTQLDIGGLPYAKVAAAIELLATEVAPVVRREIAK 356 +Q+ELFGH RF+ Q+DIGG+P+ K A IE LATEVAPV+R+E +K Sbjct: 308 HQYELFGHQRFLAQIDIGGMPFKKAAENIERLATEVAPVIRKETSK 353 >A7Z540.1 A7Z540_BACA2 Putative uncharacterized protein Length = 357 Score = 363 bits (932), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 174/348 (50%), Positives = 241/348 (69%), Gaps = 1/348 (0%) Query: 12 FEFGLYTLGDIVTDCHTGQRISPQQRMKEVIEAAKLADEAGLDVFGVGEHHRLDFVISSV 71 E G+Y+ ++ D TG+ +S +QR+ E+++ AKLAD+AGLDVFGVGEHHRLD+ +SS Sbjct: 9 MEIGIYSFAELYPDPLTGKTVSAKQRIDELMKLAKLADDAGLDVFGVGEHHRLDYAVSSP 68 Query: 72 PVVLAAISQVTRRIKLTSATTVLSTIDPVRVFEDFATLDLLSGGRAEIISGRGAFLESFP 131 P +L+AISQ+T IKLTSATTVLST DPVR+FEDFATLDL+S GRAEII+GRGAFL+SFP Sbjct: 69 PAILSAISQITENIKLTSATTVLSTADPVRLFEDFATLDLISDGRAEIIAGRGAFLDSFP 128 Query: 132 LFGYDLEDYKQLFTXXXXX-XXXXXXREVMNWQGNFRSSLKNAEIAPRPLQQKLPLWVXX 190 LFGYD+ YK+LF E + W+G +R L+ AEI+PRP + +PLW+ Sbjct: 129 LFGYDVGHYKELFEEHIDLFLKLNSGEESVTWKGRYRPPLREAEISPRPFRGSIPLWLGV 188 Query: 191 XXXXXXXXXXXXXXVGMAIAILSGSPEPFQNLADTYRRSGAEAGHAASDLKIAITSHGYI 250 G+A+A+LSG P + L D YR + ++AGH ++K+A+T H +I Sbjct: 189 SGTPESAERAGRAGAGLALAVLSGDPMSSKPLVDKYREAASKAGHPPENVKVAVTGHMFI 248 Query: 251 AKTSQQALDEYYPYYYSYRNAISPHPGQEYRVSRRDFGRFVSPVNTLAVGSPQQIIEKIL 310 +KT++QA+ E+YPY+ +Y + + + G + +++ DF R L VGS QQIIEKI+ Sbjct: 249 SKTNEQAIQEFYPYHSNYWSHVHSYHGIDEPLTKEDFERASGKETALFVGSSQQIIEKIM 308 Query: 311 YQHELFGHNRFMTQLDIGGLPYAKVAAAIELLATEVAPVVRREIAKKN 358 +Q+ELFGH RF+ Q+DIGG+P+ K A IE LA EVAPV+R+E K + Sbjct: 309 HQYELFGHQRFLAQIDIGGMPFKKAAENIERLAAEVAPVIRKETGKSS 356 >B5WQI6.1 B5WQI6_9BURK Luciferase-like monooxygenase Length = 349 Score = 320 bits (821), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 159/343 (46%), Positives = 223/343 (65%), Gaps = 4/343 (1%) Query: 12 FEFGLYTLGDIVTDCHTGQRISPQQRMKEVIEAAKLADEAGLDVFGVGEHHRLDFVISSV 71 EFG+YT GD+ + + ++R+ +++EAA ADEAG+DVFGVGEHHR DF ISS Sbjct: 1 MEFGIYTFGDLAHGASDMKHV--RERINDIVEAATFADEAGIDVFGVGEHHRPDFAISSP 58 Query: 72 PVVLAAISQVTRRIKLTSATTVLSTIDPVRVFEDFATLDLLSGGRAEIISGRGAFLESFP 131 VVLA I+ VT+RI+LTSA ++LS+ DPVR+++ F+TLDL+S GRAE++ GRGA++ESFP Sbjct: 59 SVVLATIAAVTQRIRLTSAVSILSSDDPVRLYQQFSTLDLVSAGRAELLVGRGAYIESFP 118 Query: 132 LFGYDLEDYKQLFTXXXXXXXXXXXREVMNWQGNFRSSLKNAEIAPRPLQQKLPLWVXXX 191 LFGY L DY+ LF R V+ WQG+ R +L A I PRPL LP+W+ Sbjct: 119 LFGYALVDYEALFEEKLALLQAIDERAVVTWQGHHRPALDAAAIMPRPLHGHLPIWIAAG 178 Query: 192 XXXXXXXXXXXXXVGMAIAILSGSPEPFQNLADTYRRSGAEAGHAASDLKIAITSHGYIA 251 + + +A + G PE F D YR+SG EAGH L++AI+ H ++ Sbjct: 179 GTPTSAIRAGRAGLPLNLANIGGMPERFVPFIDLYRQSGIEAGHPRDALQVAISGHFHVQ 238 Query: 252 KTSQQALDEYYPYYYSYRNAISPHPGQEYRVSRRDFGRFVSPVNTLAVGSPQQIIEKILY 311 + ++A DE++PYY SY + P +SR ++ SP L VGS Q+I++K+L Sbjct: 239 RDGRRARDEFFPYYRSY--IANNLPRGHAAMSREEYEALASPRGALFVGSVQEIVDKVLM 296 Query: 312 QHELFGHNRFMTQLDIGGLPYAKVAAAIELLATEVAPVVRREI 354 +HELFGH R+M Q+D+GG+P+ KV +IELLATEVAP VR+ + Sbjct: 297 EHELFGHQRYMAQMDVGGVPFEKVRDSIELLATEVAPAVRKAL 339 >C1XFV0.1 C1XFV0_MEIRU Flavin-dependent oxidoreductase, F420-dependent methylene-tetrahydromethanopterin reductase Length = 356 Score = 315 bits (806), Expect = 5e-84, Method: Compositional matrix adjust. Identities = 172/348 (49%), Positives = 221/348 (63%), Gaps = 3/348 (0%) Query: 12 FEFGLYTLGDIVTDCHTGQRISPQQRMKEVIEAAKLADEAGLDVFGVGEHHRLDFVISSV 71 FE GLYT + D +TGQ P QRM++++E A LAD+ GLDVF VGEHHR +++ S+ Sbjct: 6 FEIGLYTFVENTPDPYTGQLQDPAQRMRDLLEQAHLADQVGLDVFAVGEHHREEYLASAP 65 Query: 72 PVVLAAISQVTRRIKLTSATTVLSTIDPVRVFEDFATLDLLSGGRAEIISGRGAFLESFP 131 VVLAAI+ TRRI+LTSA TVLS+ DPVRVF+ FATLDLLSGGRAEI++GRG+F+ESFP Sbjct: 66 AVVLAAIAARTRRIRLTSAVTVLSSDDPVRVFQQFATLDLLSGGRAEIMAGRGSFIESFP 125 Query: 132 LFGYDLEDYKQLFTXXXXXXXXXXXREVMNWQGNFRSSLKNAEIAPRPLQQKLPLWVXXX 191 LFGYDL+DY LF +E + W G FR +L + + PRP+Q LP+WV Sbjct: 126 LFGYDLDDYSALFEEKLELLLKIRAQERVTWSGRFRPALHDQPVYPRPVQNPLPVWVAVG 185 Query: 192 XXXXXXXXXXXXXVGMAIAILSGSPEPFQNLADTYRRSGAEAGHAASDLKIAITSHGYIA 251 + MA+AI+ G PE F L D YR++ GHA L +I SHG+IA Sbjct: 186 GTPQSVVRAATLGLPMALAIIGGMPERFAPLFDLYRQTARRLGHAPQPL--SINSHGFIA 243 Query: 252 KTSQQALDEYYPYYYSYRNAISPHPGQEYRVSRRDFGRFVSPVNTLAVGSPQQIIEKILY 311 ++AL+ Y N I G + SR + VG P+QIIEKIL+ Sbjct: 244 DDPREALELAYTSSSYIMNRIGQERGWPPQ-SRAQYEASTGLRGANFVGHPEQIIEKILF 302 Query: 312 QHELFGHNRFMTQLDIGGLPYAKVAAAIELLATEVAPVVRREIAKKNS 359 QH++FGH RF+ QL +G LP+ KV AIELL TEVAPVVR+EIA K++ Sbjct: 303 QHKIFGHQRFLLQLTVGTLPHRKVLRAIELLGTEVAPVVRKEIASKSN 350 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,954,240,267 Number of extensions: 72957171 Number of successful extensions: 201314 Number of sequences better than 1.0e-05: 661 Number of HSP's gapped: 201016 Number of HSP's successfully gapped: 661 Length of query: 367 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 230 Effective length of database: 1,770,017,148 Effective search space: 407103944040 Effective search space used: 407103944040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)