BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0014.p (363 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K471.1 B3K471_9BACI Alcohol dehydrogenase zinc-binding domain ... 309 3e-82 B7R6C0.1 B7R6C0_9THEO Oxidoreductase, zinc-binding dehydrogenase... 264 8e-69 B8HUI7.1 B8HUI7_CYAP4 Oxidoreductase domain protein 254 1e-65 A6T0C4.1 A6T0C4_JANMA Oxidoreductase 251 6e-65 A8AA58.1 A8AA58_IGNH4 Alcohol dehydrogenase, zinc-binding domain... 241 7e-62 >B3K471.1 B3K471_9BACI Alcohol dehydrogenase zinc-binding domain protein Length = 341 Score = 309 bits (792), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 169/337 (50%), Positives = 231/337 (68%), Gaps = 11/337 (3%) Query: 11 MKAVVSQNGQISVADIPAPV-LEDGFVLVETEYSAISPGTEIMMNGIHRPKPVVLGYSAA 69 MKAV + +G + V I P L G V + TEYSA+SPGTE++ + V++GYSA Sbjct: 1 MKAVATIHGNVIVDHIERPAELPAGHVRIRTEYSAVSPGTEMIFIRKASDQKVIMGYSAV 60 Query: 70 GVIRSRGKGMEHWPEGQRVACYGAPYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGLGAI 129 G++ G+G++ GQRVACYGAPY +HA+WL +P +L VP+ V P+EA+ GLGAI Sbjct: 61 GIVEDLGEGVKGLAVGQRVACYGAPYVRHAQWLTVPSNLTAIVPDGVDPQEAAFAGLGAI 120 Query: 130 AVHAVRQADLQFGETLVLIGAGILGQLIAQIARAAGCRVIVYDLLAERCRTAESLGIR-H 188 A+HA+R ADL+FGE+ V++G GILGQ+IAQIA AA C V +DL ER + G+R H Sbjct: 121 AIHALRVADLRFGESAVVVGLGILGQIIAQIAHAAACPVAGFDLNPERVQ-----GMRQH 175 Query: 189 IAV---NPQRVSRHLDNLTAGMGADAVIICAGGKSGGLVDQGLEWVRNQGKVLLVGDVKP 245 IA N +++ + +T G GAD+VI+CA G L++ + W+R++GK+++VGD+ Sbjct: 176 IAYAYDNREQLEEQVGRMTGGHGADSVILCASGPGEELINASMGWIRDKGKIVIVGDLST 235 Query: 246 DFSRELMFGKEAQVLISRAGGPGRYDPVYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGD 305 +SR LMFGKEAQVLISRAGGPGRYD YE DYP G+VRWTEGRN+AEYIRL++EG Sbjct: 236 TYSRGLMFGKEAQVLISRAGGPGRYDASYELNNTDYPIGFVRWTEGRNIAEYIRLLAEGR 295 Query: 306 IRVKPLISSVFPLERSADAFRQYAESPAELLGAVLAY 342 I +KPLIS V P++ + A+ +Y + LG + Y Sbjct: 296 ISIKPLISHVVPVDEAGKAYERYGPG-STTLGTIFKY 331 >B7R6C0.1 B7R6C0_9THEO Oxidoreductase, zinc-binding dehydrogenase family Length = 344 Score = 264 bits (675), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 148/342 (43%), Positives = 208/342 (60%), Gaps = 8/342 (2%) Query: 7 ERGNMKAVVSQNGQISVADIPAPVLEDGFVLVETEYSAISPGTEIMMNGIHRPKPVVLGY 66 E M+A+++ G++ + ++ P L + +LV T YSAIS GTE+ + P LGY Sbjct: 2 EGKKMRAILTCEGKVYLDEVEEPQLGERTLLVRTLYSAISTGTELNLISSKTKNPFRLGY 61 Query: 67 SAAGVIRSRGKGMEHWPEGQRVACYGAPYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGL 126 SA GV+ G G++ + G VACYGAPY H E L +P L VP+ VS +EA+ VGL Sbjct: 62 SAVGVVEEIGNGVKGFEIGNLVACYGAPYVFHGEKLSVPITLCAKVPQGVSLKEAAFVGL 121 Query: 127 GAIAVHAVRQADLQFGETLVLIGAGILGQLIAQIARAAGCRVIVYDLLAERCRTAESLGI 186 GAIA+H +R+A+LQFGE++V++G G+LGQL QI+ A RVI E A+ Sbjct: 122 GAIAIHGIRKANLQFGESVVILGLGLLGQLSYQISAHANYRVIGVSYSNEHAEIAKKFNY 181 Query: 187 RHIAV------NPQRVSRHLDNLTAGMGADAVIICAGGKSGGLVDQGLEWVRNQGKVLLV 240 ++ V N + +++D +T G G DAV+I A L+D L+WVR GK+++V Sbjct: 182 QNGHVINLQEENEDSLLKYIDEITEGNGVDAVVITAHSPDTKLIDNALKWVRVGGKIVIV 241 Query: 241 GDVKPDFSRELMFGKEAQVLISRAGGPGRYDPVYEKQGIDYPYGYVRWTEGRNMAEYIRL 300 G+V+ +F RE F KEA+VLISRA GPGRYD YEK +DYP VRWTEGRNM EYIRL Sbjct: 242 GNVRMEFDREAFFQKEAEVLISRAAGPGRYDLNYEKNAVDYPVSLVRWTEGRNMKEYIRL 301 Query: 301 ISEGDIRVKPLISSVFPLERSADAFRQYAESPAELLGAVLAY 342 + E I + P I +P E++ + + + P +LG + Y Sbjct: 302 LKEKKIVISPFIKE-YPFEKAPEVYSSLKKEP-NILGTIFKY 341 >B8HUI7.1 B8HUI7_CYAP4 Oxidoreductase domain protein Length = 715 Score = 254 bits (649), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 146/372 (39%), Positives = 209/372 (56%), Gaps = 40/372 (10%) Query: 11 MKAVVSQNGQISVADIPAPVLEDGFVLVETEYSAISPGTE----------IMMNGIHRPK 60 MK V+ + GQ V ++PAP++E +LV+ +S IS GTE + I P+ Sbjct: 1 MKQVLIKKGQAIVEEVPAPLVEPQTLLVQVSHSCISTGTEMSGLKTSSTPLWKKAIQNPE 60 Query: 61 PVV---------------------------LGYSAAGVIRSRGKGMEHWPEGQRVACYGA 93 +V GYSAAG + + G G+E G RVAC GA Sbjct: 61 KLVKVMDMARTQGVARTASMVQGKLESGEPTGYSAAGTVLAVGSGIEDIQVGDRVACAGA 120 Query: 94 PYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAGIL 153 A HAE + +P++L P+P + ASTV LGAIA+ VR+A GET +IG G+L Sbjct: 121 QCAHHAEIIRVPRNLSAPIPAELPLSTASTVTLGAIALQGVRRAQPTLGETFTVIGLGVL 180 Query: 154 GQLIAQIARAAGCRVIVYDLLAERCRTAESLGIRHIAVNPQRVS--RHLDNLTAGMGADA 211 GQL Q+ +A GCRVI DL +R + A+ LG+ I ++P+ + + T GMG D Sbjct: 181 GQLTVQLLQANGCRVIGIDLDRKRIQLAQQLGMV-IGIHPEDCTPIEQVARYTEGMGTDG 239 Query: 212 VIICAGGKSGGLVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGRYD 271 VII A S ++ + R +G+V+LVGDV + +R + KE IS + GPGRYD Sbjct: 240 VIITAATPSDEVISTAFQMCRKKGRVVLVGDVGLNLNRADFYQKELDFFISTSYGPGRYD 299 Query: 272 PVYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAFRQYAES 331 YE++G+DYP Y+RWTE RNMAEY+RL++ G I+V+PLIS+ FP+E++ A+ S Sbjct: 300 RRYEERGLDYPLPYIRWTENRNMAEYLRLVALGKIKVEPLISATFPVEQATTAYETIRTS 359 Query: 332 PAELLGAVLAYP 343 + L +L+YP Sbjct: 360 EDKPLIVLLSYP 371 >A6T0C4.1 A6T0C4_JANMA Oxidoreductase Length = 716 Score = 251 bits (642), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 148/371 (39%), Positives = 212/371 (57%), Gaps = 38/371 (10%) Query: 11 MKAVVSQNGQISVADIPAPVLEDGFVLVETEYSAISPGTE-------------------- 50 MK V+ + G + + D+PAP +E G VLV+ + S IS GTE Sbjct: 1 MKQVLIKQGSVVLEDVPAPTVEPGSVLVQVQSSCISIGTEMSGVRATGLPLWRRALRQPE 60 Query: 51 --------IMMNGIHRPKPVV---------LGYSAAGVIRSRGKGMEHWPEGQRVACYGA 93 I G+ + + GYSAAGV+ G G++ + G RVAC GA Sbjct: 61 NVKKALAMIASQGLSKASSQINERLTAAMPTGYSAAGVVVEVGAGVDEFKVGDRVACAGA 120 Query: 94 PYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQADLQFGETLVLIGAGIL 153 A HAE++ +P++L V + + V+ + ASTV LGAIA+ VR+A GET V+IG GIL Sbjct: 121 QCAFHAEYICVPKNLAVFIDDAVNIDHASTVTLGAIALQGVRRASPTLGETFVVIGLGIL 180 Query: 154 GQLIAQIARAAGCRVIVYDLLAERCRTAESLGIR-HIAVNPQRVSRHLDNLTAGMGADAV 212 GQL Q+ +A GCRVI DL ER R A+ G+ I+ + H++ +T G+GAD V Sbjct: 181 GQLTVQLLKANGCRVIGVDLDNERVRLAQQHGMDVGISTETENDIEHVNRMTGGIGADGV 240 Query: 213 IICAGGKSGGLVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKEAQVLISRAGGPGRYDP 272 II A S ++ + R +G+ +LVGDV + +R + KE IS + GPGRYD Sbjct: 241 IITAATPSDAVISTAFKMCRKKGRTVLVGDVGLNLNRGDFYLKELDFFISSSYGPGRYDA 300 Query: 273 VYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVFPLERSADAFRQYAESP 332 YE+ G+DYP GYVRWTE RNMAEY+RLI+EG +R+ LIS+ FP++++ A+ ++ Sbjct: 301 NYEEHGLDYPIGYVRWTENRNMAEYLRLIAEGRVRLDALISTTFPVDQATAAYETLKDTE 360 Query: 333 AELLGAVLAYP 343 + L +L+YP Sbjct: 361 TKPLMVLLSYP 371 >A8AA58.1 A8AA58_IGNH4 Alcohol dehydrogenase, zinc-binding domain protein Length = 742 Score = 241 bits (616), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 143/386 (37%), Positives = 217/386 (56%), Gaps = 56/386 (14%) Query: 11 MKAVVSQNGQISVADIPAPVLEDGFVLVETEYSAISPGTEI------------MMNGIHR 58 MKAVV + ++SV ++P P + D VLV T YSAIS GTE+ +++ + Sbjct: 1 MKAVVIKGSKVSVEEVPPPRVGDNMVLVATAYSAISAGTELASLRAKKVKEGWLLDKLPL 60 Query: 59 PKPVVL----------------------------------------GYSAAGVIRSRGKG 78 PKPV + GYSA+G + +G G Sbjct: 61 PKPVRMVLENPYYARLALQKIREGGLKELLKYYQKAKEVAVKGRFSGYSASGYVIGKGSG 120 Query: 79 MEHWPEGQRVACYGAPYAKHAEWLLMPQHLMVPVPEHVSPEEASTVGLGAIAVHAVRQAD 138 + +G VA G +A HAE P+ L V +PE + +EASTV LGAIA+ +R+A+ Sbjct: 121 VIDVNKGDAVAIAGVGFASHAELNATPRRLAVKIPEGLGLKEASTVALGAIALQGIRRAE 180 Query: 139 LQFGETLVLIGAGILGQLIAQIARAAGCRVIVYDLLAERCRTAESLGIRHIAVNPQRVS- 197 L G+++ +IG G++G L AQIA++ +V+ +D+ +R A S G+ +A N V+ Sbjct: 181 LHQGDSVAIIGLGLIGNLAAQIAKSYNLKVVGFDINDKRVELARSKGV--LAFNSTSVNP 238 Query: 198 -RHLDNLTAGMGADAVIICAGGKSGGLVDQGLEWVRNQGKVLLVGDVKPDFSRELMFGKE 256 + LT G GADAVII A GL+D L+ +R++GKV+++G F R M+ K+ Sbjct: 239 VEKVMELTEGFGADAVIIAASSDKPGLIDLALDMLRHRGKVVVLGAFPMQFDRTRMYLKD 298 Query: 257 AQVLISRAGGPGRYDPVYEKQGIDYPYGYVRWTEGRNMAEYIRLISEGDIRVKPLISSVF 316 A++ IS + GPGRYDPVYE G+DYP GYVRWTE RNM EY+RL++EG + ++ ++ +V+ Sbjct: 299 AEIRISVSYGPGRYDPVYELGGVDYPIGYVRWTEERNMKEYLRLLAEGYVDLEGVVGAVY 358 Query: 317 PLERSADAFRQYAESPAELLGAVLAY 342 P+E + A++ E L A+++Y Sbjct: 359 PVEEAPKAYQDLLEGKINGLTALISY 384 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,193,257,074 Number of extensions: 98597672 Number of successful extensions: 281799 Number of sequences better than 1.0e-05: 3264 Number of HSP's gapped: 282614 Number of HSP's successfully gapped: 3264 Length of query: 363 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 226 Effective length of database: 1,770,017,148 Effective search space: 400023875448 Effective search space used: 400023875448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)