BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0009.p (253 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3JWC2.1 A3JWC2_9RHOB Dehydrogenase 174 5e-42 A1VLM8.1 A1VLM8_POLNA Short-chain dehydrogenase/reductase SDR 173 1e-41 Q5KZB7.1 Q5KZB7_GEOKA 3-oxoacyl-[acyl-carrier protein] reductase... 172 4e-41 A2SJY5.1 A2SJY5_METPP Dehydrogenase 168 3e-40 A4INJ4.1 A4INJ4_GEOTN 3-oxoacyl-(Acyl carrier protein) reductase... 168 4e-40 >A3JWC2.1 A3JWC2_9RHOB Dehydrogenase Length = 279 Score = 174 bits (442), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 96/250 (38%), Positives = 150/250 (60%), Gaps = 2/250 (0%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEV 60 M+L K V+VTGAA+G+G A RFL++GA VV+ D + +L +V +L ++ G++ V Sbjct: 20 MKLKNKTVIVTGAAQGLGYACALRFLQDGANVVMADVNAEKLAESVQDLIEFDGAVSSMV 79 Query: 61 CDLRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFRI 120 CD+ L+ A +++G +D+++NNAGIA + FL+IS + ++AV ++N+R F Sbjct: 80 CDVTKRENLQALVGLAVSNYGNLDVMINNAGIAKVQDFLEISDDEYDAVMDVNLRGAFIG 139 Query: 121 GQLAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIRV 180 Q+A +QM NMSS N + A+ L+ Y SK + LT AV LA + IRV Sbjct: 140 TQIAGKQMIAQGKGGVIINMSSINALVANPMLSTYAISKGGMNQLTNIAAVGLAPHNIRV 199 Query: 181 NAVAPGFVDTPL--DRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGE 238 + PG + T + D + + + TPM+R P+E+A+V FLAS++ASY+TG+ Sbjct: 200 CGIGPGTILTDMVADVIMTSPEARHNVLSRTPMERCGEPREIASVAAFLASDDASYMTGQ 259 Query: 239 TVRVDGGHLA 248 TV DGG +A Sbjct: 260 TVYPDGGRMA 269 >A1VLM8.1 A1VLM8_POLNA Short-chain dehydrogenase/reductase SDR Length = 260 Score = 173 bits (439), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 107/247 (43%), Positives = 141/247 (57%), Gaps = 8/247 (3%) Query: 3 LAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEVCD 62 LAG+ +VTG A+GIG A RRF EGA+VV+ D + A EL G L V CD Sbjct: 12 LAGRVCIVTGGAQGIGEACIRRFAREGAQVVVADIDDARGAALAGEL----GGLYVH-CD 66 Query: 63 LRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFRIGQ 122 + D AQ++ A+A A G ID++VNNAGI FL+++ ++AV IN++ F +GQ Sbjct: 67 VGDKAQVDALVAQAMAAHGRIDVLVNNAGIFKAADFLEVTEADFDAVLRINLKGSFLVGQ 126 Query: 123 LAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIRVNA 182 AR+M NMSS N + A +A YN SK + LT MA+ LA GIRVNA Sbjct: 127 AVAREM-AKAGQGSIVNMSSVNAVLAIPTIASYNVSKGGINQLTRVMALALADKGIRVNA 185 Query: 183 VAPGFVDTPLDRR--LREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGETV 240 VAPG + T L + L E+ TPMKR P E+A+ +LAS+ ASY+TGE V Sbjct: 186 VAPGTIATELAAKAVLTSEEAKARIMSRTPMKRLGEPSEIADTVAYLASDAASYITGEIV 245 Query: 241 RVDGGHL 247 DGG + Sbjct: 246 VADGGRM 252 >Q5KZB7.1 Q5KZB7_GEOKA 3-oxoacyl-[acyl-carrier protein] reductase (3-ketoacyl-[acyl carrier protein] reductase) Length = 256 Score = 172 bits (435), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 102/254 (40%), Positives = 149/254 (58%), Gaps = 7/254 (2%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELA--DYTGSLMV 58 MR A ++TG GIG A R +EGA VVL+ R+ +L V ++ G Sbjct: 1 MRFANTVAVITGGGSGIGRATAVRLAQEGATVVLVGRTAVKLEETVQDITRLGVPGVADR 60 Query: 59 EVCDLRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVF 118 D+ D Q+ AA +G + ++VNNAGI+ +L+++ + W+ V +N+++VF Sbjct: 61 FAADVTDHEQVRALAAYVQNRYGDLHVLVNNAGISTHTKWLELTEQEWDDVQRVNMKSVF 120 Query: 119 RIGQLAARQMXXXXX----XXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELA 174 + Q A M N++S +G A AE+ HY+A+KA VI LT+S+A+ELA Sbjct: 121 LVSQTLAPLMIEGAKRERANRAIVNVASLSGHQAGAEIPHYSAAKAGVINLTKSLALELA 180 Query: 175 RYGIRVNAVAPGFVDTPL-DRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEAS 233 YGIRVN+V+PGFV+TPL +R L+ E + + +T + R AP+E+ANV FLAS EAS Sbjct: 181 PYGIRVNSVSPGFVETPLTERGLQNERFVKAIERNTALGRVGAPEEIANVIAFLASSEAS 240 Query: 234 YVTGETVRVDGGHL 247 Y+TG V VDGG L Sbjct: 241 YMTGSDVLVDGGWL 254 >A2SJY5.1 A2SJY5_METPP Dehydrogenase Length = 261 Score = 168 bits (426), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 103/248 (41%), Positives = 139/248 (56%), Gaps = 3/248 (1%) Query: 3 LAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELADYTGSLMVEVCD 62 L+G+ +VTGAA+GIG A RF EGA V L D + LA + CD Sbjct: 8 LSGRVTVVTGAAQGIGAACAERFAREGAPVALWDVDDERGQTMAARLAAAGVQAIYRHCD 67 Query: 63 LRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVFRIGQ 122 + Q++ + A A FG ID++VNNAGI FLD++ W+AV +N++ F +GQ Sbjct: 68 VSRADQVQAAHAATLAAFGRIDVLVNNAGIFRAADFLDVTEADWDAVIGVNLKGAFLVGQ 127 Query: 123 LAARQMXXXXXXXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELARYGIRVNA 182 AR M NMSS NG+ A +A YNASK + LT +MA+ LA +G+RVNA Sbjct: 128 ACARTM-ASQGRGVIVNMSSVNGLMAIPTIASYNASKGGINQLTRAMALALADHGVRVNA 186 Query: 183 VAPGFVDTPLDRR--LREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEASYVTGETV 240 VAPG + T L + L ++ TP+KR P E+A+V FLAS+ ASY+TG+ V Sbjct: 187 VAPGTIATELATQAVLTSDEARQRILGRTPLKRLGEPAEIADVVAFLASDAASYLTGQIV 246 Query: 241 RVDGGHLA 248 DGG LA Sbjct: 247 YADGGRLA 254 >A4INJ4.1 A4INJ4_GEOTN 3-oxoacyl-(Acyl carrier protein) reductase-like protein Length = 256 Score = 168 bits (425), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 97/254 (38%), Positives = 150/254 (59%), Gaps = 7/254 (2%) Query: 1 MRLAGKRVLVTGAARGIGLAITRRFLEEGARVVLLDRSETELHAAVDELA--DYTGSLMV 58 MR + +VTG GIG A R +EGA VVL+ R+ +L V E+A + + Sbjct: 1 MRFSNTVAVVTGGGSGIGRATAIRLAQEGAHVVLVGRTAAKLEETVQEIARLEAPAAADW 60 Query: 59 EVCDLRDLAQLEQSAAKAFAHFGGIDIVVNNAGIAYREPFLDISPEHWNAVHEINVRAVF 118 D+ D Q++ A + +G + +++NNAGI+ +L+++ + W+ V +N+++VF Sbjct: 61 FAADVTDREQVKALAEYVESRYGDLHVLINNAGISTHTKWLELTEQEWDDVQRVNIKSVF 120 Query: 119 RIGQLAARQMXXXXX----XXXXXNMSSKNGIAASAELAHYNASKAAVILLTESMAVELA 174 + Q A M N++S +G A A + HY+A+KA VI LT+S+A+ELA Sbjct: 121 LVSQTLAPLMIEGAKRERVNRAIVNVASLSGHQAGAHIPHYSAAKAGVINLTKSLALELA 180 Query: 175 RYGIRVNAVAPGFVDTPL-DRRLREEDGLPSHSEHTPMKRAAAPQEVANVFLFLASEEAS 233 YGIRVN+V+PGFV+TPL ++ L+ E + + +T + R P+E+A+V FLAS EAS Sbjct: 181 PYGIRVNSVSPGFVETPLTEQGLQNERFVKAIERNTALGRVGTPEEIASVIAFLASSEAS 240 Query: 234 YVTGETVRVDGGHL 247 Y+TG V VDGG L Sbjct: 241 YMTGSDVLVDGGWL 254 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,287,666,114 Number of extensions: 46704988 Number of successful extensions: 271836 Number of sequences better than 1.0e-05: 31518 Number of HSP's gapped: 210291 Number of HSP's successfully gapped: 31631 Length of query: 253 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 120 Effective length of database: 1,807,701,208 Effective search space: 216924144960 Effective search space used: 216924144960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)