BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0008.p (279 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3ZZK2.1 A3ZZK2_9PLAN Putative uncharacterized protein 192 4e-47 A6CCX2.1 A6CCX2_9PLAN Amidohydrolase 2 186 2e-45 C1WXJ0.1 C1WXJ0_9ACTO Predicted TIM-barrel fold metal-dependent ... 179 3e-43 B5GHY6.1 B5GHY6_9ACTO Amidohydrolase 176 2e-42 A0ADQ7.1 A0ADQ7_STRAM Putative metal-dependent hydrolase of the ... 142 3e-32 >A3ZZK2.1 A3ZZK2_9PLAN Putative uncharacterized protein Length = 281 Score = 192 bits (487), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 103/280 (36%), Positives = 157/280 (56%), Gaps = 2/280 (0%) Query: 1 MIFDSHTHLFG-PGMVAGPTDAAIKRAW-GPDMNIEATPEQHGRNLEGFSGAIVLAMAAH 58 MI D H+H++ P RA G ++++ E + IV A Sbjct: 1 MIIDVHSHVWSYPQHFTDDFRNQASRARSGVEVDLTVRFEDYRATAPDDVRTIVFGGKAK 60 Query: 59 ATGLVVPNEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAAVRELGLKGLKLAPIYQNF 118 +G+ V + VAEY + P +L GF SVDP P + + + LGL+G+KL +Y F Sbjct: 61 LSGMWVDDHSVAEYVQQHPEQLIGFLSVDPTQPHWYDELQHGHQTLGLRGVKLLSMYAGF 120 Query: 119 YPDDPKHMSLYATAEQLNVPILWHQGTSFVPEGYLDASRPAMLDPIARAFPKLKMIVAHM 178 PD L+ Q +P+L H GT+F+ + LD + P LD +A +P+ +MI+AH+ Sbjct: 121 RPDSELLDPLWQYTSQHGLPVLLHTGTTFISQAPLDCTLPRYLDRVAARYPQQRMILAHL 180 Query: 179 GHPWVDECISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGVQDKLLFGSDYPFFGTQ 238 GHP+ EC++++RKH ++Y DVSAL RP+Q Y +L+ EYGV DK+LFG+DYPF Sbjct: 181 GHPYEGECVAVIRKHQHVYADVSALHYRPFQLYQSLMLVQEYGVWDKVLFGTDYPFTTVN 240 Query: 239 QMLDALYSINDLVEGTKLPHVPEDFIEAIIHRPTPEILGL 278 + + ++N LV+GT LP + E IE +I+R + +L L Sbjct: 241 DSIAGIRNLNSLVQGTSLPRLDEQKIEQMIYRDSLPLLNL 280 >A6CCX2.1 A6CCX2_9PLAN Amidohydrolase 2 Length = 295 Score = 186 bits (472), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 102/281 (36%), Positives = 157/281 (55%), Gaps = 3/281 (1%) Query: 1 MIFDSHTHLFG-PGMVAGP--TDAAIKRAWGPDMNIEATPEQHGRNLEGFSGAIVLAMAA 57 MI D H+H + P AA + G ++++ E + + IV A Sbjct: 13 MIIDVHSHAWKFPDHFNDDFRLQAARRAKAGVELDLTVEYEAYRNTAPAETRTIVFGGKA 72 Query: 58 HATGLVVPNEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAAVRELGLKGLKLAPIYQN 117 +GL V + YVA+Y P L G+ S+DP + LGLKG+KL +Y Sbjct: 73 RLSGLWVDDRYVADYVNAHPETLIGYMSLDPTQAGWQDEMREGYEVLGLKGIKLLSMYAG 132 Query: 118 FYPDDPKHMSLYATAEQLNVPILWHQGTSFVPEGYLDASRPAMLDPIARAFPKLKMIVAH 177 F PDD L+ A Q +P+L H GT+FV + LD + P +D +A FP++++++AH Sbjct: 133 FRPDDRLLDPLWEFATQNKLPVLLHTGTTFVAQAPLDCTLPIHIDRVAIRFPEVRIVMAH 192 Query: 178 MGHPWVDECISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGVQDKLLFGSDYPFFGT 237 + HP+ E + ++RKHP++Y D+SAL RP+Q Y++L+ EYGV DKLLFGSDYPF Sbjct: 193 LSHPYEGESVVVIRKHPHVYADISALHYRPFQLYHSLMLVQEYGVWDKLLFGSDYPFTTV 252 Query: 238 QQMLDALYSINDLVEGTKLPHVPEDFIEAIIHRPTPEILGL 278 ++ L ++N ++EGT LP + + IE+++ R +LGL Sbjct: 253 NASIEGLRNLNLMLEGTALPRLDTEQIESLLFRNALPLLGL 293 >C1WXJ0.1 C1WXJ0_9ACTO Predicted TIM-barrel fold metal-dependent hydrolase, COG2159 Length = 271 Score = 179 bits (453), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 86/196 (43%), Positives = 123/196 (62%), Gaps = 1/196 (0%) Query: 66 NEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAAVRELGLKGLKLAPIYQNFYPDDPKH 125 N AE+ DP R GF SV+P PD + + + RELGL G+KL P YQ+F P Sbjct: 76 NTATAEFVAADPARRIGFMSVNPTRPDALEEADRC-RELGLVGVKLGPNYQDFDPLGDAA 134 Query: 126 MSLYATAEQLNVPILWHQGTSFVPEGYLDASRPAMLDPIARAFPKLKMIVAHMGHPWVDE 185 + YA EQ +PIL+HQG S + L + P + D +A FP+L++++AHMGHPW E Sbjct: 135 LRFYAYCEQHGLPILFHQGASPIRHAPLRYTYPLVTDEVALRFPELRIVMAHMGHPWSRE 194 Query: 186 CISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGVQDKLLFGSDYPFFGTQQMLDALY 245 + +RKHP++Y DVS++ RPW Y +L++A E+G KLL GSD+P + + + L Sbjct: 195 TVVTIRKHPHVYADVSSIYLRPWVLYQSLINAVEWGAAGKLLLGSDFPIATSGEAIAGLR 254 Query: 246 SINDLVEGTKLPHVPE 261 ++ND++EGTKLP VP Sbjct: 255 TVNDMLEGTKLPRVPS 270 >B5GHY6.1 B5GHY6_9ACTO Amidohydrolase Length = 278 Score = 176 bits (447), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 101/277 (36%), Positives = 160/277 (57%), Gaps = 1/277 (0%) Query: 3 FDSHTHLFGPGMVAGPT-DAAIKRAWGPDMNIEATPEQHGRNLEGFSGAIVLAMAAHATG 61 D H+HLF T A RA ++++ E++ + + +V+ A +G Sbjct: 1 MDVHSHLFRHSHDFDATFRAESARAHAGEVDLTVRYEEYAASAPPGTRTVVVGGKARRSG 60 Query: 62 LVVPNEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAAVRELGLKGLKLAPIYQNFYPD 121 L V + VA Y P +L G+ ++DP P + +ELGL+G+K+ P+Y F P Sbjct: 61 LWVDDAAVAAYVARHPEQLIGYLAIDPTQPGWREELRYGHQELGLRGIKIMPMYAGFDPA 120 Query: 122 DPKHMSLYATAEQLNVPILWHQGTSFVPEGYLDASRPAMLDPIARAFPKLKMIVAHMGHP 181 DP++ L+ AE+ +P+L H GT+FV + L+ + P LD +A P L+M++AH+ HP Sbjct: 121 DPEYDDLFGYAEEHGLPLLVHTGTTFVSQAPLEYAMPRHLDRVAIRHPGLRMVLAHLSHP 180 Query: 182 WVDECISLVRKHPNLYMDVSALGSRPWQFYNALVSAAEYGVQDKLLFGSDYPFFGTQQML 241 + ECI+++RKHP++Y D+SAL RP+Q +++L +YGV DKLLFGSDYPF + Sbjct: 181 FEGECIAVIRKHPHVYADISALHYRPFQLWHSLRLVQDYGVWDKLLFGSDYPFTTVNDSI 240 Query: 242 DALYSINDLVEGTKLPHVPEDFIEAIIHRPTPEILGL 278 + L I + L + +D +EA+IHRP ++LGL Sbjct: 241 EGLRRIARVPGIPGLDPLDQDAVEALIHRPALDLLGL 277 >A0ADQ7.1 A0ADQ7_STRAM Putative metal-dependent hydrolase of the TIM-barrel fold Length = 257 Score = 142 bits (358), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 75/178 (42%), Positives = 101/178 (56%), Gaps = 6/178 (3%) Query: 61 GLVVPNEYVAEYCRTDPGRLFGFASVDPNDPDCVGKFEAAVRELGLKGLKLAPIYQNFYP 120 G VPNE+VAE C P R GFASVD P + AV LGL GLK AP Y P Sbjct: 21 GFSVPNEFVAELCARHPDRFTGFASVDAGTPGAAARLRHAVTALGLSGLKTAPCYLRMSP 80 Query: 121 DDPKHMSLYATAEQLNVPILWHQGTSFVPEGYLDAS--RPAMLDPIARAFPKLKMIVAHM 178 D + +Y TA+ L VP+L H G + P D P +++ +A+ FP L +I+AH+ Sbjct: 81 ADRRWYDVYETAQDLGVPVLMHTG--YTPSRNADPRYFSPLLVEQVAKDFPGLTVILAHL 138 Query: 179 GHPWVDECISLVRKHPNLYMDVSALGSR--PWQFYNALVSAAEYGVQDKLLFGSDYPF 234 G PW +C+ L+ + P LY D+S GS P +AL A + GV D+LL+G+D+PF Sbjct: 139 GTPWTRQCVDLLARRPRLYADLSIFGSYQPPATVASALAYARDSGVLDRLLWGTDFPF 196 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,865,236,312 Number of extensions: 79477316 Number of successful extensions: 161995 Number of sequences better than 1.0e-05: 356 Number of HSP's gapped: 161637 Number of HSP's successfully gapped: 356 Length of query: 279 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 145 Effective length of database: 1,798,280,193 Effective search space: 260750627985 Effective search space used: 260750627985 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)