BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0007.p (593 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q01U60.1 Q01U60_SOLUE Dihydroxyacid dehydratase EC=4.2.1.9 644 0.0 B1G0E7.1 B1G0E7_9BURK Dihydroxy-acid dehydratase EC=4.2.1.9 628 e-178 A9IQ59.1 A9IQ59_BORPD Dihydroxy-acid dehydratase EC=4.2.1.9 627 e-177 Q7WKV5.1 Q7WKV5_BORBR Putative dihydroxy-acid dehydratase EC=4.2... 622 e-176 Q7W7G6.1 Q7W7G6_BORPA Putative dihydroxy-acid dehydratase EC=4.2... 620 e-175 >Q01U60.1 Q01U60_SOLUE Dihydroxyacid dehydratase EC=4.2.1.9 Length = 565 Score = 644 bits (1660), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/558 (56%), Positives = 413/558 (74%), Gaps = 4/558 (0%) Query: 21 RLKSESYFREKDLWGFIHRSFTKSMGYTEEDLR-KPVIGICNTFSELNKCHSHFNELANY 79 +L+S S+F K+ + F R+ +++G + E KPVIGICN+FSELN C++H ++A+ Sbjct: 8 KLRSYSWFNGKEYYNFSRRANIRALGISREAFHGKPVIGICNSFSELNNCNAHLRQVADA 67 Query: 80 VKRGVWQAGGVPMEFPTISIGEPYVKPTTMLLRNLMAMDTEEMMKGHPIDGVVLLXXXGG 139 VKRGVW AGG+P+EFP IS+GE Y+KPTTML RNLMAMD EE ++ +P+DGVVLL G Sbjct: 68 VKRGVWAAGGLPLEFPVISLGEQYMKPTTMLFRNLMAMDVEECIRANPVDGVVLL---AG 124 Query: 140 CDKTVPAQLMAAASVNLPAIVLTGGPMLNGRLDGRSLGACTDCYGFTLEHKAGNLTDEEL 199 CDKT PA LM AAS ++PA+++TGGP L+G G +G TD ++ G ++ EEL Sbjct: 125 CDKTTPAMLMGAASADIPAMIVTGGPTLSGNYQGTPIGQGTDGRKIFDLYRMGRVSSEEL 184 Query: 200 AVAEDAICRSDGHCMVMGTASTMASIAEALGMALPGCAAIPAPDSRRRHLSERTGKQIVE 259 E + RS GHC VMGTASTMA +AEALGM+LPG AA+PAPDSRR ++E +G+++VE Sbjct: 185 QEIEGCMVRSAGHCTVMGTASTMAIVAEALGMSLPGSAAVPAPDSRRLAMAEMSGRRMVE 244 Query: 260 LVRRDIRPRDIMTAEAVENAITVTMASGGSTNAIIHLVAISQRLGRKLPLETFDAISRRT 319 +VR D+RP ++T +A ENAITV MA GGSTNA++HL+AI+ R KL L+ FDAISRRT Sbjct: 245 MVREDLRPSRVLTRQAFENAITVDMAVGGSTNAVVHLLAIAGRTPNKLVLDDFDAISRRT 304 Query: 320 PFILNLRPSGKYQMEEYFEAGGVPALMKELEPLLHGGCLTVTGKTVTENLVQAATLDREV 379 PF++N+RPSGK+ ME++F AGGVPA+M+EL PLLH CLTV GKTV EN+ +A +R+V Sbjct: 305 PFLVNVRPSGKHMMEDFFFAGGVPAVMRELLPLLHRECLTVNGKTVGENVGEAPCYNRDV 364 Query: 380 IRSMAEPLESQGGIAVLRGNLAPDGALIKQTAVSAHLKQHTGRAVVFESRADLLLRIDDP 439 IR+ + L ++GG VLRGNL PDGA++KQTA SA L +HTG A VFES + ID Sbjct: 365 IRTPDQALFAEGGTVVLRGNLCPDGAVLKQTAASAELCEHTGPAYVFESYQAMHDSIDRM 424 Query: 440 ELEVDEHSVLVLKNAGPKGAPAMPEIGQIPIPQKLLVRGIRDMVRISDARISGTSYGALI 499 +L +D +VLV+K GPKGAP MPE G IP+P+ LL RG+ D VRISDAR+SGTS+G ++ Sbjct: 425 DLPIDRDTVLVMKGCGPKGAPGMPEWGHIPMPKVLLERGVSDCVRISDARMSGTSFGTVV 484 Query: 500 VHAAPEAAVGGTLALVRDGDEIELDIAARKLVLKVPDRELARRRALWKAPEPHYDRGYGL 559 +H +PE+ VGG LA+V GD I L +AARKL L + + EL RR AL + PHY RGYG Sbjct: 485 LHVSPESGVGGPLAVVETGDRITLSVAARKLHLHITEEELRRRTALLQPAAPHYSRGYGK 544 Query: 560 LFHERVLQANLGCDFDFL 577 LF + V QA+LGCDFDFL Sbjct: 545 LFLDHVTQAHLGCDFDFL 562 >B1G0E7.1 B1G0E7_9BURK Dihydroxy-acid dehydratase EC=4.2.1.9 Length = 590 Score = 628 bits (1620), Expect = e-178, Method: Compositional matrix adjust. Identities = 309/564 (54%), Positives = 404/564 (71%), Gaps = 4/564 (0%) Query: 15 SRAAGHRLKSESYFREKDLWGFIHRSFTKSMGYTEED-LRKPVIGICNTFSELNKCHSHF 73 +R +L+S ++ DL F HRS T MGY D + KPVI + NT+SE+N CH+HF Sbjct: 3 NRKTSEQLRSYRWYGVNDLRSFGHRSRTAQMGYHASDYMGKPVIAVVNTWSEINSCHTHF 62 Query: 74 NELANYVKRGVWQAGGVPMEFPTISIGEPYVKPTTMLLRNLMAMDTEEMMKGHPIDGVVL 133 + VKRG+WQAGG P+E P +++ EP+ KPTTML RN +AM+TEE++K +P DG VL Sbjct: 63 KQRVEEVKRGIWQAGGFPVEMPVMTLAEPFQKPTTMLYRNFLAMETEEILKSYPFDGCVL 122 Query: 134 LXXXGGCDKTVPAQLMAAASVNLPAIVLTGGPMLNGRLDGRSLGACTDCYGFTLEHKAGN 193 + GGCDKT P LM A S+NLPAI L GPML G +GR+LG+ +D + + E +AGN Sbjct: 123 M---GGCDKTTPGLLMGAISMNLPAIFLPAGPMLRGNWNGRTLGSGSDTWKYWAELRAGN 179 Query: 194 LTDEELAVAEDAICRSDGHCMVMGTASTMASIAEALGMALPGCAAIPAPDSRRRHLSERT 253 +T++E E I RS GHCM MGTASTM S AEALG+ LPG ++IPA DSR + T Sbjct: 180 ITEDEWKGIESGIARSPGHCMTMGTASTMTSAAEALGLTLPGFSSIPAVDSRHAQFASLT 239 Query: 254 GKQIVELVRRDIRPRDIMTAEAVENAITVTMASGGSTNAIIHLVAISQRLGRKLPLETFD 313 G++IVE+V D++P DI+TA++ +NA+T +A GSTNAI+HLVA+++R G L FD Sbjct: 240 GQRIVEMVWTDLKPSDILTAKSFDNAVTTVLAMSGSTNAIVHLVAVARRAGIDLTTARFD 299 Query: 314 AISRRTPFILNLRPSGKYQMEEYFEAGGVPALMKELEPLLHGGCLTVTGKTVTENLVQAA 373 +SR TP I NLRPSG+Y ME++F AGG+ AL+ EL L+ G LTV G T+ EN+ A Sbjct: 300 ELSRITPVIGNLRPSGQYLMEDFFYAGGLRALLVELGDLIDGSQLTVNGATLGENISGAE 359 Query: 374 TLDREVIRSMAEPLESQGGIAVLRGNLAPDGALIKQTAVSAHLKQHTGRAVVFESRADLL 433 + +VIR + P+ + G+AVL GNLAPDGA+IK A+ AHL +H G AVVF+ AD+ Sbjct: 360 IFNDDVIRRRSNPVVASDGLAVLTGNLAPDGAVIKPAAMEAHLLKHRGPAVVFKDYADMA 419 Query: 434 LRIDDPELEVDEHSVLVLKNAGPKGAPAMPEIGQIPIPQKLLVRGIRDMVRISDARISGT 493 RID EL+V SV+VL++AGP GAP MPE GQ+PIPQKLL +G+RDMVRISDAR+SGT Sbjct: 420 ARIDMEELDVSADSVIVLQHAGPVGAPGMPEWGQLPIPQKLLKQGVRDMVRISDARMSGT 479 Query: 494 SYGALIVHAAPEAAVGGTLALVRDGDEIELDIAARKLVLKVPDRELARRRALWKAPEPHY 553 SYGA ++H APE+ VGG LALV+DGD IELD+AAR+L L V D ELA R+A W+ P+ + Sbjct: 480 SYGACVLHVAPESFVGGPLALVKDGDMIELDVAARRLHLDVTDEELAARKAAWQPPKLPF 539 Query: 554 DRGYGLLFHERVLQANLGCDFDFL 577 +RG+G++ VLQAN GCDFDFL Sbjct: 540 ERGFGVMHQLHVLQANKGCDFDFL 563 >A9IQ59.1 A9IQ59_BORPD Dihydroxy-acid dehydratase EC=4.2.1.9 Length = 581 Score = 627 bits (1617), Expect = e-177, Method: Compositional matrix adjust. Identities = 305/557 (54%), Positives = 404/557 (72%), Gaps = 4/557 (0%) Query: 22 LKSESYFREKDLWGFIHRSFTKSMGYTEEDLR-KPVIGICNTFSELNKCHSHFNELANYV 80 L+S ++ KDL F HRS T MGY D KPVI I NT+S++N CHSHF + V Sbjct: 13 LRSHRWYGVKDLRSFGHRSRTAQMGYHRSDYAGKPVIAIINTWSDINPCHSHFKQRVEEV 72 Query: 81 KRGVWQAGGVPMEFPTISIGEPYVKPTTMLLRNLMAMDTEEMMKGHPIDGVVLLXXXGGC 140 KRG+WQAGG P+E P +S+ EP+ KPTTML RNL+AM+TEE+++ +P DG VL+ GGC Sbjct: 73 KRGIWQAGGFPVEMPAMSLSEPFQKPTTMLYRNLLAMETEELLRSYPADGCVLM---GGC 129 Query: 141 DKTVPAQLMAAASVNLPAIVLTGGPMLNGRLDGRSLGACTDCYGFTLEHKAGNLTDEELA 200 DKT PA LM AAS++LP I + GPML G +G++LG+ +D + + E +AGN+T+E+ Sbjct: 130 DKTTPALLMGAASMDLPTIFVPAGPMLRGNWNGQTLGSGSDTWKYWAELRAGNITEEDWQ 189 Query: 201 VAEDAICRSDGHCMVMGTASTMASIAEALGMALPGCAAIPAPDSRRRHLSERTGKQIVEL 260 ED I RS GHCM MGTASTM + EALGM L G ++IPAPDSR ++ +GK+IVE+ Sbjct: 190 GVEDGIARSPGHCMTMGTASTMTAAVEALGMCLSGYSSIPAPDSRHAQMATLSGKRIVEM 249 Query: 261 VRRDIRPRDIMTAEAVENAITVTMASGGSTNAIIHLVAISQRLGRKLPLETFDAISRRTP 320 V +DI P ++TA + +NA+ +A GSTNA++HL+A+++R G L L+ FD ++R TP Sbjct: 250 VWQDITPSSLLTAASFDNAVRTVLALSGSTNAVVHLIAMARRCGFPLDLDRFDNLARSTP 309 Query: 321 FILNLRPSGKYQMEEYFEAGGVPALMKELEPLLHGGCLTVTGKTVTENLVQAATLDREVI 380 + NLRPSGKY ME+++ AGG+ ALM +L LL TV G+T+ EN+ A +VI Sbjct: 310 VLANLRPSGKYLMEDFYYAGGLRALMVQLGDLLDTTQQTVDGRTLGENIAGARVFHEDVI 369 Query: 381 RSMAEPLESQGGIAVLRGNLAPDGALIKQTAVSAHLKQHTGRAVVFESRADLLLRIDDPE 440 RS + L G+AVLRGNLAPDGA+IK A+ A L+ HTGRAVVF+ D+ RIDDP Sbjct: 370 RSREQALVPCDGLAVLRGNLAPDGAVIKPPAMEARLQTHTGRAVVFKDYNDMAARIDDPN 429 Query: 441 LEVDEHSVLVLKNAGPKGAPAMPEIGQIPIPQKLLVRGIRDMVRISDARISGTSYGALIV 500 L+VD SV+VL+NAGP+GAP MPE GQ+PIPQKLL +G+RDM+RISDAR+SGTSYGA ++ Sbjct: 430 LDVDADSVIVLQNAGPQGAPGMPEWGQLPIPQKLLKQGVRDMLRISDARMSGTSYGACVL 489 Query: 501 HAAPEAAVGGTLALVRDGDEIELDIAARKLVLKVPDRELARRRALWKAPEPHYDRGYGLL 560 H APE+ VGG LALVRDGD I LD+ R+L +++ D ELA RRA W+AP P ++RGYG+L Sbjct: 490 HVAPESYVGGPLALVRDGDLITLDVPNRRLDVRLSDAELAERRAAWQAPPPRFERGYGVL 549 Query: 561 FHERVLQANLGCDFDFL 577 + + + QA+ GCDFDFL Sbjct: 550 YLKHISQADSGCDFDFL 566 >Q7WKV5.1 Q7WKV5_BORBR Putative dihydroxy-acid dehydratase EC=4.2.1.9 Length = 581 Score = 622 bits (1603), Expect = e-176, Method: Compositional matrix adjust. Identities = 301/567 (53%), Positives = 405/567 (71%), Gaps = 4/567 (0%) Query: 12 NAESRAAGHRLKSESYFREKDLWGFIHRSFTKSMGYTEEDLR-KPVIGICNTFSELNKCH 70 A R L+S ++ KDL F HRS T MGY D KPVI I NT+S++N CH Sbjct: 3 QAPKRKRPEDLRSHRWYGVKDLRAFGHRSRTAQMGYHRSDYAGKPVIAIINTWSDINPCH 62 Query: 71 SHFNELANYVKRGVWQAGGVPMEFPTISIGEPYVKPTTMLLRNLMAMDTEEMMKGHPIDG 130 SHF + VKRG+WQAGG P+E P +S+ EP+ KPTTML RNL+AM+TEE+++ +P DG Sbjct: 63 SHFKQRVEEVKRGIWQAGGFPVEMPAMSLSEPFQKPTTMLYRNLLAMETEELLRSYPADG 122 Query: 131 VVLLXXXGGCDKTVPAQLMAAASVNLPAIVLTGGPMLNGRLDGRSLGACTDCYGFTLEHK 190 VL+ GGCDKT PA LM A S+ LP + + GPML G +G +LG+ +D + + E + Sbjct: 123 CVLM---GGCDKTTPALLMGAVSMGLPTVFVPAGPMLRGNWNGNTLGSGSDTWKYWAELR 179 Query: 191 AGNLTDEELAVAEDAICRSDGHCMVMGTASTMASIAEALGMALPGCAAIPAPDSRRRHLS 250 AGN+T+E+ ED I RS GHCM MGTASTM + EALG+ L G ++IPAPDSR ++ Sbjct: 180 AGNITEEDWQGVEDGIARSPGHCMTMGTASTMTAAVEALGLCLSGYSSIPAPDSRHAQMA 239 Query: 251 ERTGKQIVELVRRDIRPRDIMTAEAVENAITVTMASGGSTNAIIHLVAISQRLGRKLPLE 310 TGK+IVE+V D++P DI+TA + +NA+ +A GSTNA++HL+A+++R G L LE Sbjct: 240 SLTGKRIVEMVWEDLKPADILTAASFDNAVRTVLALSGSTNAVVHLIALARRAGFGLDLE 299 Query: 311 TFDAISRRTPFILNLRPSGKYQMEEYFEAGGVPALMKELEPLLHGGCLTVTGKTVTENLV 370 FD ++R TP + NLRP+G+Y ME+++ AGG+ AL+ +L LL +TV G+T+ EN+ Sbjct: 300 RFDHLARSTPVLANLRPAGQYLMEDFYYAGGLRALLAQLGDLLDTRQMTVDGRTLGENIA 359 Query: 371 QAATLDREVIRSMAEPLESQGGIAVLRGNLAPDGALIKQTAVSAHLKQHTGRAVVFESRA 430 A + EVIRS L + G+AVLRGNLAPDGA+IK A+ A L++HTGRAVVF+ Sbjct: 360 GARVFNDEVIRSRERALIERDGLAVLRGNLAPDGAVIKPAAMEARLQRHTGRAVVFKDYN 419 Query: 431 DLLLRIDDPELEVDEHSVLVLKNAGPKGAPAMPEIGQIPIPQKLLVRGIRDMVRISDARI 490 D+ RID P+L++D SV+VL+NAGP+GAP MPE GQ+PIPQKLL +G+RDMVRISDAR+ Sbjct: 420 DMAERIDAPDLDIDADSVIVLQNAGPQGAPGMPEWGQLPIPQKLLKQGVRDMVRISDARM 479 Query: 491 SGTSYGALIVHAAPEAAVGGTLALVRDGDEIELDIAARKLVLKVPDRELARRRALWKAPE 550 SGTSYGA ++H APEA VGG LALVR GD I LD+ R++ + + D ELA R+A W+AP Sbjct: 480 SGTSYGACVLHVAPEAYVGGPLALVRSGDRIALDVPGRRIDVLISDEELAARKAAWQAPP 539 Query: 551 PHYDRGYGLLFHERVLQANLGCDFDFL 577 P ++RG+G+L+ + + QA+ GCDFDFL Sbjct: 540 PKFERGFGVLYLKHIGQADSGCDFDFL 566 >Q7W7G6.1 Q7W7G6_BORPA Putative dihydroxy-acid dehydratase EC=4.2.1.9 Length = 581 Score = 620 bits (1600), Expect = e-175, Method: Compositional matrix adjust. Identities = 300/567 (52%), Positives = 404/567 (71%), Gaps = 4/567 (0%) Query: 12 NAESRAAGHRLKSESYFREKDLWGFIHRSFTKSMGYTEEDLR-KPVIGICNTFSELNKCH 70 A R L+S ++ KDL F HRS T MGY D KPVI I NT+S++N CH Sbjct: 3 QAPKRKRPEDLRSHRWYGVKDLRAFGHRSRTAQMGYHRSDYAGKPVIAIINTWSDINPCH 62 Query: 71 SHFNELANYVKRGVWQAGGVPMEFPTISIGEPYVKPTTMLLRNLMAMDTEEMMKGHPIDG 130 SHF + VKRG+WQAGG P+E P +S+ EP+ KPTTML RNL+AM+TEE+++ +P DG Sbjct: 63 SHFKQRVEEVKRGIWQAGGFPVEMPAMSLSEPFQKPTTMLYRNLLAMETEELLRSYPADG 122 Query: 131 VVLLXXXGGCDKTVPAQLMAAASVNLPAIVLTGGPMLNGRLDGRSLGACTDCYGFTLEHK 190 VL+ GGCDKT PA LM A S+ LP + + GPML G +G +LG+ +D + + E + Sbjct: 123 CVLM---GGCDKTTPALLMGAVSMGLPTVFVPAGPMLRGNWNGNTLGSGSDTWKYWAELR 179 Query: 191 AGNLTDEELAVAEDAICRSDGHCMVMGTASTMASIAEALGMALPGCAAIPAPDSRRRHLS 250 AGN+T+E+ ED I RS GHCM MGTASTM + EALG+ L G ++IPAPDSR ++ Sbjct: 180 AGNITEEDWQGVEDGIARSPGHCMTMGTASTMTAAVEALGLCLSGYSSIPAPDSRHAQMA 239 Query: 251 ERTGKQIVELVRRDIRPRDIMTAEAVENAITVTMASGGSTNAIIHLVAISQRLGRKLPLE 310 TGK+IVE+V D++P DI+TA + +NA+ +A GSTNA++HL+A+++R G L LE Sbjct: 240 SLTGKRIVEMVWEDLKPADILTAASFDNAVRTVLALSGSTNAVVHLIALARRAGFGLDLE 299 Query: 311 TFDAISRRTPFILNLRPSGKYQMEEYFEAGGVPALMKELEPLLHGGCLTVTGKTVTENLV 370 FD ++R TP + NLRP+G+Y ME+++ AGG+ AL+ +L LL +TV G+T+ EN+ Sbjct: 300 RFDHLARSTPVLANLRPAGQYLMEDFYYAGGLRALLAQLGDLLDTRQMTVDGRTLGENIA 359 Query: 371 QAATLDREVIRSMAEPLESQGGIAVLRGNLAPDGALIKQTAVSAHLKQHTGRAVVFESRA 430 A + EVIRS L + G+AVLRGNLAPDGA+IK A+ A L++HTGRAVVF+ Sbjct: 360 GARVFNEEVIRSRERALIERDGLAVLRGNLAPDGAVIKPAAMEARLQRHTGRAVVFKDYN 419 Query: 431 DLLLRIDDPELEVDEHSVLVLKNAGPKGAPAMPEIGQIPIPQKLLVRGIRDMVRISDARI 490 D+ RID P+L++D SV+VL+NAGP+GAP MPE GQ+PIPQKLL +G+RDMVRISDAR+ Sbjct: 420 DMAERIDAPDLDIDADSVIVLQNAGPQGAPGMPEWGQLPIPQKLLKQGVRDMVRISDARM 479 Query: 491 SGTSYGALIVHAAPEAAVGGTLALVRDGDEIELDIAARKLVLKVPDRELARRRALWKAPE 550 SG SYGA ++H APEA VGG LALVR GD I LD+ R++ + + D ELA R+A W+AP Sbjct: 480 SGASYGACVLHVAPEAYVGGPLALVRSGDRIALDVPGRRIDVLISDEELAARKAAWQAPP 539 Query: 551 PHYDRGYGLLFHERVLQANLGCDFDFL 577 P ++RG+G+L+ + + QA+ GCDFDFL Sbjct: 540 PKFERGFGVLYLKHIGQADSGCDFDFL 566 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,551,475,072 Number of extensions: 150557731 Number of successful extensions: 401233 Number of sequences better than 1.0e-05: 2557 Number of HSP's gapped: 387914 Number of HSP's successfully gapped: 3126 Length of query: 593 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 451 Effective length of database: 1,722,912,073 Effective search space: 777033344923 Effective search space used: 777033344923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)