BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0373.0006.p (346 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K462.1 B3K462_9BACI NAD-dependent epimerase/dehydratase 475 e-132 B7R6B5.1 B7R6B5_9THEO NAD dependent epimerase/dehydratase family... 452 e-125 C1U3C0.1 C1U3C0_9SPHI Nucleoside-diphosphate-sugar epimerase 436 e-120 Q2BFB7.1 Q2BFB7_9BACI Putative uncharacterized protein 432 e-119 C1Z147.1 C1Z147_PEDHE NAD dependent epimerase/dehydratase family... 404 e-111 >B3K462.1 B3K462_9BACI NAD-dependent epimerase/dehydratase Length = 338 Score = 475 bits (1222), Expect = e-132, Method: Compositional matrix adjust. Identities = 217/336 (64%), Positives = 264/336 (78%) Query: 11 SVQELEDRLARPSAALLEELARVDGDIMLLGVGGKMGPSLARLAVNAIREAGLDKKVIGV 70 +V LE +LA PSA L+ +LA ++GDI++LGVGGKMGPSLARLA NA+RE G K++IGV Sbjct: 3 TVAALEAKLAEPSAQLVADLASLEGDILVLGVGGKMGPSLARLAANAVREGGGGKRIIGV 62 Query: 71 SRFSNDALKQELLEAGVEIISADLSDDSALQALPETKNILYMAGNKFGTTGNEHFTWMMN 130 SRFSN ++EL EAGV+ IS DL +D LQ LPE N++YMAGNKFGTTG E+FTW MN Sbjct: 63 SRFSNQEARRELEEAGVQTISCDLLNDGELQQLPEAPNVIYMAGNKFGTTGREYFTWAMN 122 Query: 131 AYLPGRVAERFRNSRIVVFSTGNVYPLTPVGQGGATERTSPNANGEYGQSCLGRERIFEH 190 +YLPGRVAE+F+ SR+VVFS+GNVYP +PVG GG E +P GEY QSCLGRER+FE+ Sbjct: 123 SYLPGRVAEKFKQSRMVVFSSGNVYPFSPVGSGGVNESVAPEPVGEYAQSCLGRERVFEY 182 Query: 191 FSHKNGTPMFIYRLNYAIDLRYGVLLELARSVKEQRPIDITMGHANVIWQGDANELALRA 250 FSH+NGTPM IYRLNYAID+RYGVL ELARS+ E RPI + MGHAN+IWQGDANE+ALR+ Sbjct: 183 FSHQNGTPMAIYRLNYAIDMRYGVLHELARSIHEDRPIHLAMGHANIIWQGDANEMALRS 242 Query: 251 LLRCSSPPNVMNITGPETISLRWAATEIGRRLGVEPEFTGEEXXXXXXXXXXXXXXXFGY 310 LL CSSPP ++NITGPET+S+RWAA E+ R+G E FTG E FGY Sbjct: 243 LLHCSSPPEIINITGPETMSIRWAAQELAARMGKEVTFTGTESETALLNNASKSHQLFGY 302 Query: 311 PQVTLAQMIDWVAEWVRLDGATWNKPTHFQEREGKY 346 P+V+L QM+DW A WV+ G +WNKPTHFQER+GK+ Sbjct: 303 PKVSLLQMLDWTAAWVQSGGESWNKPTHFQERKGKF 338 >B7R6B5.1 B7R6B5_9THEO NAD dependent epimerase/dehydratase family protein Length = 373 Score = 452 bits (1163), Expect = e-125, Method: Compositional matrix adjust. Identities = 209/338 (61%), Positives = 261/338 (77%) Query: 9 IRSVQELEDRLARPSAALLEELARVDGDIMLLGVGGKMGPSLARLAVNAIREAGLDKKVI 68 + +V+ELE +L+ PS AL+ E+A+++GDIM+LGVGGKMGPSLA+LA NAI +A +DKKVI Sbjct: 36 MHTVEELEAKLSEPSQALISEIAQLEGDIMILGVGGKMGPSLAKLAKNAIDKAKVDKKVI 95 Query: 69 GVSRFSNDALKQELLEAGVEIISADLSDDSALQALPETKNILYMAGNKFGTTGNEHFTWM 128 GVSRFSN L++EL + G++ I+ADL +D LQ+LPE KN+++MAG+KFGTTGNEHFTW Sbjct: 96 GVSRFSNKELQRELEDYGIQTINADLLEDDQLQSLPEVKNVIFMAGHKFGTTGNEHFTWA 155 Query: 129 MNAYLPGRVAERFRNSRIVVFSTGNVYPLTPVGQGGATERTSPNANGEYGQSCLGRERIF 188 MNAYLPG+VA++F+NSRIVVFS+GNVYPLTPV GGA+E GEY QS LGRER+F Sbjct: 156 MNAYLPGQVAKKFKNSRIVVFSSGNVYPLTPVFMGGASEEHPTGPVGEYAQSVLGRERVF 215 Query: 189 EHFSHKNGTPMFIYRLNYAIDLRYGVLLELARSVKEQRPIDITMGHANVIWQGDANELAL 248 E+FS K PM I RLNYAIDLRYGVL E+A VKEQ+PID+ MGH NVIWQGDANE+AL Sbjct: 216 EYFSRKYNIPMLIMRLNYAIDLRYGVLHEIATLVKEQKPIDLRMGHVNVIWQGDANEIAL 275 Query: 249 RALLRCSSPPNVMNITGPETISLRWAATEIGRRLGVEPEFTGEEXXXXXXXXXXXXXXXF 308 R+L C PP ++N+TGPETIS+RW A G + G+ P F EE F Sbjct: 276 RSLRFCEVPPKILNVTGPETISIRWLAERFGEKFGIRPVFVNEEEDTALLSNASKAHYLF 335 Query: 309 GYPQVTLAQMIDWVAEWVRLDGATWNKPTHFQEREGKY 346 GYP+V+L QMI+W AEW++ G + KPTHFQEREGK+ Sbjct: 336 GYPRVSLRQMIEWTAEWIKSGGVSLGKPTHFQEREGKF 373 >C1U3C0.1 C1U3C0_9SPHI Nucleoside-diphosphate-sugar epimerase Length = 338 Score = 436 bits (1120), Expect = e-120, Method: Compositional matrix adjust. Identities = 200/338 (59%), Positives = 253/338 (74%) Query: 9 IRSVQELEDRLARPSAALLEELARVDGDIMLLGVGGKMGPSLARLAVNAIREAGLDKKVI 68 + ++ +E++ PS AL+E++ R+DGDIM+LG GGK+GPS+ARL + AI AGLDK+VI Sbjct: 1 MNGIESIEEQWLSPSEALVEDMKRIDGDIMILGAGGKIGPSIARLTMQAIERAGLDKRVI 60 Query: 69 GVSRFSNDALKQELLEAGVEIISADLSDDSALQALPETKNILYMAGNKFGTTGNEHFTWM 128 VSRFS + L +EL G+E I A++ DD+ LQ LPE +N+LY+AG KFGT GNE +TW Sbjct: 61 AVSRFSEEGLVEELNAEGIETIVANILDDTQLQDLPEVENVLYLAGQKFGTAGNEPYTWA 120 Query: 129 MNAYLPGRVAERFRNSRIVVFSTGNVYPLTPVGQGGATERTSPNANGEYGQSCLGRERIF 188 MN YLPGRVAE+FR SRIVV+STGNVYP TPVG GGATE + GEYGQSCLGRER+F Sbjct: 121 MNTYLPGRVAEKFRKSRIVVYSTGNVYPFTPVGAGGATEDMRADPVGEYGQSCLGRERMF 180 Query: 189 EHFSHKNGTPMFIYRLNYAIDLRYGVLLELARSVKEQRPIDITMGHANVIWQGDANELAL 248 ++FS GTP+ +YRLNYAID +YGVLLE+A+SV +P+DITMGH NVIWQGDANE+A+ Sbjct: 181 QYFSSVYGTPILLYRLNYAIDFKYGVLLEVAKSVLNGKPLDITMGHVNVIWQGDANEMAI 240 Query: 249 RALLRCSSPPNVMNITGPETISLRWAATEIGRRLGVEPEFTGEEXXXXXXXXXXXXXXXF 308 R+LL C SP ++NITGPET+SLRW A E GR +EP+ GEE F Sbjct: 241 RSLLHCESPSKILNITGPETVSLRWLAMEFGRIFNIEPQLVGEEQSTALLSNAAESFRLF 300 Query: 309 GYPQVTLAQMIDWVAEWVRLDGATWNKPTHFQEREGKY 346 GYP+ TL QMI+ A+W+ G T NKPTHFQER+G++ Sbjct: 301 GYPRTTLKQMIEITAQWLLEGGKTINKPTHFQERKGQF 338 >Q2BFB7.1 Q2BFB7_9BACI Putative uncharacterized protein Length = 341 Score = 432 bits (1112), Expect = e-119, Method: Compositional matrix adjust. Identities = 196/338 (57%), Positives = 253/338 (74%) Query: 9 IRSVQELEDRLARPSAALLEELARVDGDIMLLGVGGKMGPSLARLAVNAIREAGLDKKVI 68 ++++ E+E+ + PS L+ +++R+DGDIM+LG GGKMGP+LA+LA A++ + K+VI Sbjct: 4 MKTIAEMEEVMTNPSQELVNDISRLDGDIMILGAGGKMGPTLAKLAAKAVQRSDTGKRVI 63 Query: 69 GVSRFSNDALKQELLEAGVEIISADLSDDSALQALPETKNILYMAGNKFGTTGNEHFTWM 128 VSRFS+ L++EL EAG+E I+ADL ++ L LP+ KN++YMAGNKFGT GNEHFTW Sbjct: 64 AVSRFSSGTLQKELEEAGIETIAADLLNEEELNGLPDIKNVIYMAGNKFGTNGNEHFTWA 123 Query: 129 MNAYLPGRVAERFRNSRIVVFSTGNVYPLTPVGQGGATERTSPNANGEYGQSCLGRERIF 188 MNAYLPGRVA+RFRNSRIV FSTGNVYPL P+ +GG E GEY QSCLGRERIF Sbjct: 124 MNAYLPGRVADRFRNSRIVAFSTGNVYPLVPLTKGGCDEEYPVGPVGEYAQSCLGRERIF 183 Query: 189 EHFSHKNGTPMFIYRLNYAIDLRYGVLLELARSVKEQRPIDITMGHANVIWQGDANELAL 248 +FS KN TP+ I+RLNYAID+RYGVLLE+AR V +++P+D++MGH NVIWQGDANE AL Sbjct: 184 TYFSSKNQTPVTIFRLNYAIDMRYGVLLEIARQVYDEKPVDLSMGHVNVIWQGDANEYAL 243 Query: 249 RALLRCSSPPNVMNITGPETISLRWAATEIGRRLGVEPEFTGEEXXXXXXXXXXXXXXXF 308 R+LL C SPP+++N+TGPET+S+RW A + + + F EE F Sbjct: 244 RSLLICDSPPSLLNVTGPETVSVRWLAQQFSKTFNKQAVFINEEQPSALLNNSGKAHRLF 303 Query: 309 GYPQVTLAQMIDWVAEWVRLDGATWNKPTHFQEREGKY 346 GYP+V+L QMI W AEWV DGA NKPTHFQ+R+G Y Sbjct: 304 GYPKVSLEQMIMWTAEWVSGDGAVLNKPTHFQQRQGVY 341 >C1Z147.1 C1Z147_PEDHE NAD dependent epimerase/dehydratase family protein Length = 338 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 190/326 (58%), Positives = 242/326 (74%) Query: 21 RPSAALLEELARVDGDIMLLGVGGKMGPSLARLAVNAIREAGLDKKVIGVSRFSNDALKQ 80 +PS L+ +L +++GDIMLLGVGGKMGPS+A+LA AI E+GL K++IGVSRFS+ A ++ Sbjct: 13 KPSDRLIADLRKIEGDIMLLGVGGKMGPSMAKLAKLAIDESGLKKRIIGVSRFSDTAARE 72 Query: 81 ELLEAGVEIISADLSDDSALQALPETKNILYMAGNKFGTTGNEHFTWMMNAYLPGRVAER 140 +L AG+E ISADL ++ L ALP+ NI+Y+AG KFGTTG E FTW MNAYLPGRVAER Sbjct: 73 DLEAAGIETISADLLNEDDLAALPDAANIIYLAGTKFGTTGKEAFTWAMNAYLPGRVAER 132 Query: 141 FRNSRIVVFSTGNVYPLTPVGQGGATERTSPNANGEYGQSCLGRERIFEHFSHKNGTPMF 200 F+ S IVVFSTGNVYP TPV GG +E GEYGQSCLGRERIF+HFS KN TP+ Sbjct: 133 FKGSNIVVFSTGNVYPFTPVTSGGLSEDHPVAPVGEYGQSCLGRERIFQHFSEKNNTPVL 192 Query: 201 IYRLNYAIDLRYGVLLELARSVKEQRPIDITMGHANVIWQGDANELALRALLRCSSPPNV 260 IYRLNYAIDLRYGVLLE+A++V E R ID+T G+ NVIWQGDANE+A+R+LL CS+P V Sbjct: 193 IYRLNYAIDLRYGVLLEIAKAVNEGRAIDLTTGNVNVIWQGDANEIAIRSLLHCSTPAKV 252 Query: 261 MNITGPETISLRWAATEIGRRLGVEPEFTGEEXXXXXXXXXXXXXXXFGYPQVTLAQMID 320 +N+TGPET+S++W A + G + + F E FGYP+VT+ +M++ Sbjct: 253 LNVTGPETLSVKWLAEQFGLLMNKQALFENEVQPTALLNNASEAHKLFGYPRVTIREMLE 312 Query: 321 WVAEWVRLDGATWNKPTHFQEREGKY 346 W++ G NKPTHFQER+G++ Sbjct: 313 MTVSWLQGGGKISNKPTHFQERKGQF 338 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,074,800,651 Number of extensions: 84005546 Number of successful extensions: 201376 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 201357 Number of HSP's successfully gapped: 30 Length of query: 346 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 210 Effective length of database: 1,779,438,163 Effective search space: 373682014230 Effective search space used: 373682014230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)