BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0058.p (156 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEB1.1 B3KEB1_9BACI D-isomer specific 2-hydroxyacid dehydrogen... 181 2e-44 C1PE15.1 C1PE15_BACCO D-isomer specific 2-hydroxyacid dehydrogen... 165 1e-39 Q8ES01.1 Q8ES01_OCEIH Dehydrogenase 165 1e-39 Q2BEZ9.1 Q2BEZ9_9BACI Dehydrogenase 160 5e-38 A6CNS0.1 A6CNS0_9BACI Dehydrogenase 158 2e-37 >B3KEB1.1 B3KEB1_9BACI D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding Length = 317 Score = 181 bits (458), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 82/156 (52%), Positives = 111/156 (71%) Query: 1 MTTIGVSRSGKPVEGFDEVYTTDRLQDAVSQGDFLINTLPLTDETRLMFGADIFAACKQG 60 M T+GV SGK + +Y D+L +A++QGD+++N LPLTDETR +F F+A K Sbjct: 162 MRTVGVRNSGKSDPYVESMYKVDQLDEALAQGDYIVNILPLTDETRGLFNTARFSAMKVS 221 Query: 61 SYYINIGRGATTDTQALMDALNSGHLAGAGLDVFETEPLPEDHPLWGMEQVVITPHCAGV 120 ++++N+GRG T T ++ AL SG LAGAGLDVFE EPLP DHPLW ++ V++TPH AG Sbjct: 222 AFFVNVGRGQTVVTDDMVQALQSGGLAGAGLDVFEEEPLPADHPLWNLDNVIMTPHMAGD 281 Query: 121 TDRYADRVVEIFTENMKSYLKSGTPSRNLVDYSRQY 156 TDRY +R V+IF EN+K Y+ SRN+VDYS+ Y Sbjct: 282 TDRYGERAVDIFLENLKRYVNGEPLSRNVVDYSKSY 317 >C1PE15.1 C1PE15_BACCO D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding Length = 320 Score = 165 bits (418), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 80/157 (50%), Positives = 103/157 (65%), Gaps = 1/157 (0%) Query: 1 MTTIGVSRSGKPVEGFDEVYTTDRLQDAVSQGDFLINTLPLTDETRLMFGADIFAACKQG 60 M +GV RSG P E DE+YT +L D + D++ LPLT ET +FG + FA K Sbjct: 164 MKVLGVRRSGAPAEFVDEMYTNGQLHDMLPLCDYVAVCLPLTKETYHLFGKEAFARMKPS 223 Query: 61 SYYINIGRGATTDTQALMDALNSGHLAGAGLDVFETEPLPEDHPLWGMEQVVITPHCAGV 120 +++INIGRG + + L DAL SG +AGAGLDVFE EPLPE+ PLW +E V+ITPH AG Sbjct: 224 AFFINIGRGEIANEKELADALASGEIAGAGLDVFEKEPLPENSPLWELENVIITPHTAGS 283 Query: 121 TDRYADRVV-EIFTENMKSYLKSGTPSRNLVDYSRQY 156 T+ Y +RVV +IF N+K YL P +LVDY + Y Sbjct: 284 TEHYDERVVRDIFIPNLKRYLAGDKPDIHLVDYKKGY 320 >Q8ES01.1 Q8ES01_OCEIH Dehydrogenase Length = 317 Score = 165 bits (418), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 79/157 (50%), Positives = 102/157 (64%), Gaps = 1/157 (0%) Query: 1 MTTIGVSRSGKPVEGFDEVYTTDRLQDAVSQGDFLINTLPLTDETRLMFGADIFAACKQG 60 M +GV SG+P+E DE+YT L + Q DF++ TLPLT +T MFG + FA K Sbjct: 161 MKVLGVRHSGRPLEYVDEMYTPADLSLVLEQSDFVVVTLPLTKDTHHMFGKEQFAQMKNS 220 Query: 61 SYYINIGRGATTDTQALMDALNSGHLAGAGLDVFETEPLPEDHPLWGMEQVVITPHCAGV 120 +++INIGRG D AL+ AL +AGAGLDVFETEPL D PLW ME V+ITPH AG Sbjct: 221 AFFINIGRGPIVDESALISALQQKEIAGAGLDVFETEPLQSDSPLWEMENVIITPHSAGA 280 Query: 121 TDRYADRVV-EIFTENMKSYLKSGTPSRNLVDYSRQY 156 T+ Y +RV+ +IF N+KSYL G P+ N+ + Y Sbjct: 281 TEYYTNRVIHDIFIPNLKSYLDKGEPTINVFQHKLGY 317 >Q2BEZ9.1 Q2BEZ9_9BACI Dehydrogenase Length = 313 Score = 160 bits (404), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 77/157 (49%), Positives = 101/157 (64%), Gaps = 1/157 (0%) Query: 1 MTTIGVSRSGKPVEGFDEVYTTDRLQDAVSQGDFLINTLPLTDETRLMFGADIFAACKQG 60 M IGV SGK + FDE++T ++L D + D+++ TLPLTDET +F A F K Sbjct: 157 MKVIGVRLSGKEEQHFDEMHTAEKLDDILPHCDYIVITLPLTDETHSLFSASQFKRMKNT 216 Query: 61 SYYINIGRGATTDTQALMDALNSGHLAGAGLDVFETEPLPEDHPLWGMEQVVITPHCAGV 120 ++ INIGRG Q L+ AL G +AGAGLDVFE EPL E PLW ME V+ITPH +G Sbjct: 217 AFLINIGRGEILVEQDLISALKEGEIAGAGLDVFEKEPLQESSPLWEMENVIITPHTSGS 276 Query: 121 TDRYADRVV-EIFTENMKSYLKSGTPSRNLVDYSRQY 156 T+ Y +RV+ +I N+K YL+ P+ NLVDY + Y Sbjct: 277 TEYYDERVINDILVPNLKEYLEGKKPAHNLVDYRKGY 313 >A6CNS0.1 A6CNS0_9BACI Dehydrogenase Length = 318 Score = 158 bits (399), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 77/157 (49%), Positives = 103/157 (65%), Gaps = 1/157 (0%) Query: 1 MTTIGVSRSGKPVEGFDEVYTTDRLQDAVSQGDFLINTLPLTDETRLMFGADIFAACKQG 60 M +GV SGK E DE++ TD+L D + + D+++ T+PLT ETR MFG F K Sbjct: 162 MNVLGVRHSGKENEYVDEMFKTDQLHDVLPRCDYIVITVPLTPETRGMFGQKEFQLMKDT 221 Query: 61 SYYINIGRGATTDTQALMDALNSGHLAGAGLDVFETEPLPEDHPLWGMEQVVITPHCAGV 120 ++ INIGRG T + L++AL + +AGAGLDVF EPL +D+PLW ME V+ITPH AG Sbjct: 222 AFLINIGRGETVNEAQLINALQTKKIAGAGLDVFVQEPLEKDNPLWEMENVIITPHTAGS 281 Query: 121 TDRYADRVV-EIFTENMKSYLKSGTPSRNLVDYSRQY 156 T+ Y RV+ +IF N+K Y+ PS NLVDY + Y Sbjct: 282 TEFYNKRVIDDIFIPNLKEYIAGRKPSVNLVDYQKGY 318 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 943,011,565 Number of extensions: 38555857 Number of successful extensions: 92344 Number of sequences better than 1.0e-05: 5402 Number of HSP's gapped: 90877 Number of HSP's successfully gapped: 5404 Length of query: 156 Length of database: 3,060,696,203 Length adjustment: 117 Effective length of query: 39 Effective length of database: 1,958,437,448 Effective search space: 76379060472 Effective search space used: 76379060472 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)