BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0057.p (157 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEB1.1 B3KEB1_9BACI D-isomer specific 2-hydroxyacid dehydrogen... 139 1e-31 A6CNS0.1 A6CNS0_9BACI Dehydrogenase 109 6e-23 Q2BEZ9.1 Q2BEZ9_9BACI Dehydrogenase 109 7e-23 C1PE15.1 C1PE15_BACCO D-isomer specific 2-hydroxyacid dehydrogen... 109 7e-23 A8U5K1.1 A8U5K1_9LACT D-isomer specific 2-hydroxyacid dehydrogen... 95 2e-18 >B3KEB1.1 B3KEB1_9BACI D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding Length = 317 Score = 139 bits (349), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 66/133 (49%), Positives = 90/133 (67%) Query: 1 LQKLSHRQQDLIRDAAPGYTLTLGDGKAPDLAQLAEAEIVIGWGKGISDTLLSAESPLRW 60 + L+ QQ I++ AP Y L + K + + AEI+IGW + + D +LSA+S L+W Sbjct: 7 IHDLTAEQQQKIKEIAPDYDLLVTKAKELTGSIVRNAEIMIGWSRSMQDEVLSADSKLKW 66 Query: 61 VQAWSAGVEKLPLAAMENKGILLTNASGVHAEPITAVIFGFMLLFTRNLHTAVRNQLNRR 120 +QAWSAGV+K+PL +E KGI LTNASGVH+ PIT IF +L F RNLH A+R Q N + Sbjct: 67 IQAWSAGVDKMPLRELEEKGIQLTNASGVHSVPITEHIFAMILAFNRNLHLAIRQQSNNK 126 Query: 121 WHSEGSESELTGK 133 W + G+ +ELTGK Sbjct: 127 WDTSGTFTELTGK 139 >A6CNS0.1 A6CNS0_9BACI Dehydrogenase Length = 318 Score = 109 bits (273), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 55/132 (41%), Positives = 75/132 (56%) Query: 2 QKLSHRQQDLIRDAAPGYTLTLGDGKAPDLAQLAEAEIVIGWGKGISDTLLSAESPLRWV 61 Q + Q + I+ P + + G L EAEI+ GW K + L + LRW+ Sbjct: 8 QNIEKEQTEHIQKIVPHWEVITGKEPEHWENHLGEAEIIAGWKKSMETKTLQNPAALRWI 67 Query: 62 QAWSAGVEKLPLAAMENKGILLTNASGVHAEPITAVIFGFMLLFTRNLHTAVRNQLNRRW 121 Q WSAGV+ LPL ME+K +L+T+A+GVHA PI+ IF ML TR +HT VRNQ + W Sbjct: 68 QTWSAGVDSLPLMQMESKDVLVTSANGVHAYPISETIFAMMLGLTRKIHTYVRNQAQKEW 127 Query: 122 HSEGSESELTGK 133 G + E+ GK Sbjct: 128 AHSGLKLEIHGK 139 >Q2BEZ9.1 Q2BEZ9_9BACI Dehydrogenase Length = 313 Score = 109 bits (273), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 55/125 (44%), Positives = 78/125 (62%), Gaps = 8/125 (6%) Query: 12 IRDAAPGYTLTLGDGKAPDL--AQLAEAEIVIGWGKGISDTLLSAESP-LRWVQAWSAGV 68 +++ PG+ + GK P++ + L +AEI+ GW K DT+ +A+S LRW+Q WSAGV Sbjct: 15 VKNVMPGWNII--SGKDPEIWKSHLMDAEIIAGWKK---DTVQAADSNNLRWIQTWSAGV 69 Query: 69 EKLPLAAMENKGILLTNASGVHAEPITAVIFGFMLLFTRNLHTAVRNQLNRRWHSEGSES 128 LPL +E + LT+A+GVHA PI+ IFG ML TR +HT VRNQ ++WH Sbjct: 70 NNLPLEELEKNNVFLTSANGVHAYPISETIFGLMLALTRKIHTYVRNQEEKKWHHSHMNQ 129 Query: 129 ELTGK 133 E+ K Sbjct: 130 EIHKK 134 >C1PE15.1 C1PE15_BACCO D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding Length = 320 Score = 109 bits (273), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 54/124 (43%), Positives = 74/124 (59%) Query: 10 DLIRDAAPGYTLTLGDGKAPDLAQLAEAEIVIGWGKGISDTLLSAESPLRWVQAWSAGVE 69 D IR P + + G A ++ +AE++ + K + LS +S LRWVQ+WSAGV Sbjct: 18 DEIRKLVPEWRILAGKDPAHWQNEVKKAEVIASFRKKMEKDALSEDSALRWVQSWSAGVN 77 Query: 70 KLPLAAMENKGILLTNASGVHAEPITAVIFGFMLLFTRNLHTAVRNQLNRRWHSEGSESE 129 PL E KG+ LT+ASGVHA PI+ IF ML TR +HT VRNQ+ ++W G + E Sbjct: 78 SFPLEQFEKKGLFLTSASGVHAYPISETIFAMMLALTRKIHTYVRNQMKKKWDHAGLKLE 137 Query: 130 LTGK 133 + GK Sbjct: 138 MHGK 141 >A8U5K1.1 A8U5K1_9LACT D-isomer specific 2-hydroxyacid dehydrogenase family protein Length = 320 Score = 94.7 bits (234), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 50/134 (37%), Positives = 74/134 (55%), Gaps = 1/134 (0%) Query: 1 LQKLSHRQQDLIRDAAPGYTLTLGDGKAPDLAQLAEAEIVIGWGKGISDTLLSAESP-LR 59 +QK ++ Q + ++D AP Y L G L EI+ GW ++ LL+ + P LR Sbjct: 9 IQKTTNEQIETLKDLAPDYDLIKGWETDEKEVSLESVEIIYGWTGQRTEELLADDKPVLR 68 Query: 60 WVQAWSAGVEKLPLAAMENKGILLTNASGVHAEPITAVIFGFMLLFTRNLHTAVRNQLNR 119 W Q +AGV+ L L +E K ILLTN SG+H+ PI +FG +L + R + AV++Q + Sbjct: 69 WAQGKAAGVDFLDLKKLEEKQILLTNGSGIHSIPIAESVFGLLLAYARGIQKAVKDQQTK 128 Query: 120 RWHSEGSESELTGK 133 +W EL GK Sbjct: 129 KWDQVDKLMELNGK 142 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 880,523,051 Number of extensions: 31892065 Number of successful extensions: 57873 Number of sequences better than 1.0e-05: 109 Number of HSP's gapped: 57909 Number of HSP's successfully gapped: 109 Length of query: 157 Length of database: 3,060,696,203 Length adjustment: 118 Effective length of query: 39 Effective length of database: 1,949,016,433 Effective search space: 76011640887 Effective search space used: 76011640887 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 125 (52.8 bits)