BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0044.p (488 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KE70.1 B3KE70_9BACI Phospholipase D/Transphosphatidylase 532 e-149 Q9K8Z4.1 CLS_BACHD Cardiolipin synthetase EC=2.7.8.- 400 e-109 O66043.1 CLS_BACPF Cardiolipin synthetase EC=2.7.8.- 394 e-108 C3J1X0.1 C3J1X0_9BACI Phospholipase D/Transphosphatidylase 393 e-107 B7YB40.1 B7YB40_9BACI Phospholipase D/Transphosphatidylase 393 e-107 >B3KE70.1 B3KE70_9BACI Phospholipase D/Transphosphatidylase Length = 476 Score = 532 bits (1370), Expect = e-149, Method: Compositional matrix adjust. Identities = 254/455 (55%), Positives = 331/455 (72%) Query: 34 EFRRPQRALAWLFIAFCCPPAGLLFYYFFGRDYWQSRKLHKRCISLFREIRAHLSGKIKL 93 EFR P +A+AWLFI FC P G + Y+F +DY + K+ KR LF+EIRAHL + + Sbjct: 22 EFRNPSKAVAWLFILFCLPFIGFVLYFFVAQDYQKRLKIRKRGSRLFQEIRAHLWDRAAV 81 Query: 94 VADAADSGNPHFADHKXXXXXXXXXXXXPVTGRNKCQVLFDGREAFEAMLDAMEAARDNI 153 V D NP F + P+TG N+ +VL DG E +EAML+AME A+D+I Sbjct: 82 VRRKGDMNNPGFHHNDRLFNLLNHLSESPITGCNQSRVLTDGEETYEAMLNAMEEAKDHI 141 Query: 154 HMEFYIFRDDEIGERFQDVMIRKSRQGVRVRLLCDGLGSHKLGRRFIRTLRNAGVEVHFF 213 H+EFYIFR D IG +FQDVMIRK+++GV+VRL+CDGLGS+KL + FI+ + +GVE +FF Sbjct: 142 HVEFYIFRHDRIGTKFQDVMIRKAQEGVKVRLVCDGLGSYKLKKTFIQRFKESGVEFYFF 201 Query: 214 LPPLISLLDRRFNFRNHRKIIVVDGRVGFTGGLNIGDDYLGKDPKLGYWRDTHLRLEGDA 273 LP ++S+ RR N+RNHRKI+VVDG +GF GG+N+GDDYLG+ P +GYWRDTHL ++GDA Sbjct: 202 LPAMVSMFSRRINYRNHRKIVVVDGTIGFLGGINVGDDYLGEYPNMGYWRDTHLEVKGDA 261 Query: 274 VYSLQYIFLKDWRLATGEGMNHPRFFPAHKCTDQEAVLMVASGPDGALDASQEMYFASIV 333 VY LQ +FL DW+LA+GE + FP H+CT +E V +++SGPD +A QEM F +I Sbjct: 262 VYFLQNVFLHDWKLASGERIADFGLFPEHQCTGEEQVQILSSGPDLNWNAIQEMCFGAIS 321 Query: 334 AAKQRIWITSPYFIPDPTICRALKNAVLSGVDVRIIIPAIPDNRLVYYASLSYLENLQDA 393 AK+RIWIT+PYFIPD I ALK A +SGVDVRIIIP D+RLV ASLSY+E L A Sbjct: 322 VAKERIWITTPYFIPDEGIYEALKTAAVSGVDVRIIIPDHADSRLVKMASLSYVEELLAA 381 Query: 394 GVKFYRYQKGFMHAKVMIIDDLLGTVGSANLDMRSFYSNFELTAVLLRPEVIAGLAASVE 453 GVKFY Y+KGF+HAKV+I+D+LLG+VG+AN+DMRSF+ NFELTAVL I L + + Sbjct: 382 GVKFYEYEKGFIHAKVLIVDELLGSVGTANMDMRSFFCNFELTAVLFDRLPIERLVSDFK 441 Query: 454 QDLQHSEFIEPVIFRRRGNHVKLIESLCQLLSPLL 488 +DLQHS I+ +FR+RG K E +C++LSPLL Sbjct: 442 EDLQHSRLIDAEVFRQRGRWQKAAEIMCRMLSPLL 476 >Q9K8Z4.1 CLS_BACHD Cardiolipin synthetase EC=2.7.8.- Length = 503 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 195/457 (42%), Positives = 290/457 (63%), Gaps = 6/457 (1%) Query: 34 EFRRPQRALAWLFIAFCCPPAGLLFYYFFGRDYWQSRKLHKRCISLFREIRAHLSGKIKL 93 E R P + L WL + P G +FY FG+++ +S+ K+ +S E + G +L Sbjct: 51 ENRHPTKTLTWLMVLAVFPVVGFIFYLMFGQNHRKSKTFMKKALSD-EEAFEKIEGNRQL 109 Query: 94 VADAADSGNPHFADHKXXXXXXXXXXXXPVTGRNKCQVLFDGREAFEAMLDAMEAARDNI 153 + H + P++ +VL DG+E F + A+ A +I Sbjct: 110 NEEQLQKMGGH---QQLLFRLAHRLANNPISFSTNTKVLTDGKETFAHIKQALRMATHHI 166 Query: 154 HMEFYIFRDDEIGERFQDVMIRKSRQGVRVRLLCDGLGSHKLGRRFIRTLRNAGVEVHFF 213 H+E+YI RDDEIG+ ++++++K+++G+ VR L DG+GS KL + +I+ L+ AGVE+ F Sbjct: 167 HLEYYIVRDDEIGQEIKEILMQKAKEGIHVRFLYDGVGSWKLSKSYIQDLKQAGVEIVPF 226 Query: 214 LPPLISLLDRRFNFRNHRKIIVVDGRVGFTGGLNIGDDYLGKDPKLGYWRDTHLRLEGDA 273 P + ++ N+RNHRKIIV+DG VGF GGLNIGD+YLGKDP G+WRDTHL + G+A Sbjct: 227 APVKLPFINHTINYRNHRKIIVIDGTVGFVGGLNIGDEYLGKDPYFGFWRDTHLYVRGEA 286 Query: 274 VYSLQYIFLKDWRLATGEGMNHPRFF-PA-HKCTDQEAVLMVASGPDGALDASQEMYFAS 331 V +LQ IFL+DW TGE + P + PA D V M+ASGPD + +++++F+ Sbjct: 287 VRTLQLIFLRDWAHETGETILKPSYLSPALTNMKDDGGVQMIASGPDTRWEINKKLFFSM 346 Query: 332 IVAAKQRIWITSPYFIPDPTICRALKNAVLSGVDVRIIIPAIPDNRLVYYASLSYLENLQ 391 I +AK+ IWITSPYFIPD I ALK A LSG+DVRI++P PD R+V++AS SY L Sbjct: 347 ITSAKKSIWITSPYFIPDEDILSALKIAALSGIDVRILVPNRPDKRIVFHASRSYFPELL 406 Query: 392 DAGVKFYRYQKGFMHAKVMIIDDLLGTVGSANLDMRSFYSNFELTAVLLRPEVIAGLAAS 451 +AGVK Y Y +GF+H+K++I+D+ + ++G++N+DMRSF+ NFE+ A L R + + L + Sbjct: 407 EAGVKVYEYTRGFLHSKIIIVDNEIASIGTSNMDMRSFHLNFEVNAFLYRTKSVTTLVSD 466 Query: 452 VEQDLQHSEFIEPVIFRRRGNHVKLIESLCQLLSPLL 488 DL+H+ I FR R + +++ES C+LLSPLL Sbjct: 467 FVYDLEHTNQIRFEQFRNRAWYYRVLESTCRLLSPLL 503 >O66043.1 CLS_BACPF Cardiolipin synthetase EC=2.7.8.- Length = 503 Score = 394 bits (1013), Expect = e-108, Method: Compositional matrix adjust. Identities = 196/457 (42%), Positives = 287/457 (62%), Gaps = 6/457 (1%) Query: 34 EFRRPQRALAWLFIAFCCPPAGLLFYYFFGRDYWQSRKLHKRCISLFREIRAHLSGKIKL 93 E R P + L WL + P G FY FG+++ +S++ K+ I R + + G+ +L Sbjct: 51 ENRHPTKTLTWLLVLAAFPVVGFFFYLMFGQNHRKSKRFSKKAIEDERAFQK-IEGQRQL 109 Query: 94 VADAADSGNPHFADHKXXXXXXXXXXXXPVTGRNKCQVLFDGREAFEAMLDAMEAARDNI 153 + H + P++ ++ +VL DG+E + +L A++ A +I Sbjct: 110 NEEQLKKMGGH---QQLLFRLAHKLGKNPISFSSETKVLTDGKETYAHILQALKMAEHHI 166 Query: 154 HMEFYIFRDDEIGERFQDVMIRKSRQGVRVRLLCDGLGSHKLGRRFIRTLRNAGVEVHFF 213 H+E+YI R D++G + +D++I K+++GV VR L DG+GS KL + ++ LR+AGVE+ F Sbjct: 167 HLEYYIVRHDDLGNQIKDILISKAKEGVHVRFLYDGVGSWKLSKSYVEELRDAGVEMVSF 226 Query: 214 LPPLISLLDRRFNFRNHRKIIVVDGRVGFTGGLNIGDDYLGKDPKLGYWRDTHLRLEGDA 273 P + L N+RNHRKIIV+DG VGF GGLNIGD+YLGKD GYWRDTHL + G+A Sbjct: 227 SPVKLPFLTHTINYRNHRKIIVIDGVVGFVGGLNIGDEYLGKDAYFGYWRDTHLYVRGEA 286 Query: 274 VYSLQYIFLKDWRLATGEG-MNHPRFFPAHKCTDQE-AVLMVASGPDGALDASQEMYFAS 331 V +LQ IFL+DW TGE +N P+ T + V M+ASGPD + +++++F+ Sbjct: 287 VRTLQLIFLQDWHYQTGETILNQTYLSPSLSMTKGDGGVQMIASGPDTRWEVNKKLFFSM 346 Query: 332 IVAAKQRIWITSPYFIPDPTICRALKNAVLSGVDVRIIIPAIPDNRLVYYASLSYLENLQ 391 I +AK+ IWI SPYFIPD I ALK A LSG+DVRI++P PD R+V++AS SY L Sbjct: 347 ITSAKKSIWIASPYFIPDDDILSALKIAALSGIDVRILVPNRPDKRIVFHASRSYFPELL 406 Query: 392 DAGVKFYRYQKGFMHAKVMIIDDLLGTVGSANLDMRSFYSNFELTAVLLRPEVIAGLAAS 451 +AGVK Y Y +GFMH+K++I+D + ++G++N+DMRSF+ NFE+ A L R + L + Sbjct: 407 EAGVKVYEYNRGFMHSKIIIVDHEIASIGTSNMDMRSFHLNFEVNAYLYRTSSVTKLVSD 466 Query: 452 VEQDLQHSEFIEPVIFRRRGNHVKLIESLCQLLSPLL 488 DL+HS I +F+ R +LIES +LLSPLL Sbjct: 467 YVYDLEHSNQINFSLFKNRPFFHRLIESTSRLLSPLL 503 >C3J1X0.1 C3J1X0_9BACI Phospholipase D/Transphosphatidylase Length = 502 Score = 393 bits (1010), Expect = e-107, Method: Compositional matrix adjust. Identities = 204/490 (41%), Positives = 292/490 (59%), Gaps = 7/490 (1%) Query: 1 VAYYKKYLEGEGLKIFAFIXXXXXXXXXXXXXXEFRRPQRALAWLFIAFCCPPAGLLFYY 60 +A + EG+ L +F+ + E R+P + +AWL + P G LFY Sbjct: 18 LALTNGFWEGKLLGLFSVLMSCSVIFIALVISLENRKPAQTIAWLAVLGSFPIVGFLFYL 77 Query: 61 FFGRDYWQSRKLHKRCISLFREIRAHLSGKIKLVADAADSGNPHFADHKXXXXXXXXXXX 120 FGR+YWQ R+ K+ + + K + + A P + Sbjct: 78 LFGRNYWQQRRYKKKA-----DFDEAVLLKFQEPSPIAVERLPMAPHQRPLLRLAYRIGQ 132 Query: 121 XPVTGRNKCQVLFDGREAFEAMLDAMEAARDNIHMEFYIFRDDEIGERFQDVMIRKSRQG 180 PV+ ++ VL +G E F ++ +E A +IH+E+YI R DEIG++ + V++ K+RQG Sbjct: 133 HPVSLASQTAVLTNGEETFSSIFAELEKAEHHIHLEYYIVRHDEIGQQLKRVLMEKARQG 192 Query: 181 VRVRLLCDGLGSHKLGRRFIRTLRNAGVEVHFFLPPLISLLDRRFNFRNHRKIIVVDGRV 240 VRVR L D +GS KL +I LR AGVE+ F P + L + NFRNHRKIIV+DG V Sbjct: 193 VRVRFLYDAVGSWKLSNAYIEELRAAGVEMIPFSPVRLPFLSNQINFRNHRKIIVIDGGV 252 Query: 241 GFTGGLNIGDDYLGKDPKLGYWRDTHLRLEGDAVYSLQYIFLKDWRLATGEGMNHPRFF- 299 GF GGLNIGD+YLGK+ G+WRDTHL + G+AV +LQ IFL+DW TGE + P + Sbjct: 253 GFVGGLNIGDEYLGKNKYFGFWRDTHLLIRGEAVRTLQLIFLQDWYYMTGERLLTPDYLS 312 Query: 300 -PAHKCTDQEAVLMVASGPDGALDASQEMYFASIVAAKQRIWITSPYFIPDPTICRALKN 358 P Q V ++A GPD + +++YFA I +AKQ IW+ SPYF+PD I ALK Sbjct: 313 PPLIVEEGQGGVQLIAGGPDQKWEVIKQLYFAMITSAKQSIWVASPYFVPDEDILTALKV 372 Query: 359 AVLSGVDVRIIIPAIPDNRLVYYASLSYLENLQDAGVKFYRYQKGFMHAKVMIIDDLLGT 418 A LSG+DVR++ P PD ++V+YAS SY L +AGVK Y Y+KGF+H+KV+++D L + Sbjct: 373 AALSGIDVRLLAPKRPDKKIVFYASRSYFPELLEAGVKIYEYEKGFLHSKVIVVDGELAS 432 Query: 419 VGSANLDMRSFYSNFELTAVLLRPEVIAGLAASVEQDLQHSEFIEPVIFRRRGNHVKLIE 478 +G+AN+DMRSF+ NFE+ A L + I L +D +H+ I+ F++R V++ E Sbjct: 433 IGTANMDMRSFHLNFEVNAFLYYTDSIHKLVRDFLEDFRHASMIDYEQFQQRPFRVRIAE 492 Query: 479 SLCQLLSPLL 488 S+ +LLSPLL Sbjct: 493 SVSRLLSPLL 502 >B7YB40.1 B7YB40_9BACI Phospholipase D/Transphosphatidylase Length = 502 Score = 393 bits (1010), Expect = e-107, Method: Compositional matrix adjust. Identities = 204/490 (41%), Positives = 292/490 (59%), Gaps = 7/490 (1%) Query: 1 VAYYKKYLEGEGLKIFAFIXXXXXXXXXXXXXXEFRRPQRALAWLFIAFCCPPAGLLFYY 60 +A + EG+ L +F+ + E R+P + +AWL + P G LFY Sbjct: 18 LALTNGFWEGKLLGLFSVLMSCSVIFIALVISLENRKPAQTIAWLAVLGSFPIVGFLFYL 77 Query: 61 FFGRDYWQSRKLHKRCISLFREIRAHLSGKIKLVADAADSGNPHFADHKXXXXXXXXXXX 120 FGR+YWQ R+ K+ + + K + + A P + Sbjct: 78 LFGRNYWQQRRYKKKA-----DFDEAVLLKFQEPSPIAVERLPMAPHQRPLLRLAYRIGQ 132 Query: 121 XPVTGRNKCQVLFDGREAFEAMLDAMEAARDNIHMEFYIFRDDEIGERFQDVMIRKSRQG 180 PV+ ++ VL +G E F ++ +E A +IH+E+YI R DEIG++ + V++ K+RQG Sbjct: 133 HPVSLASQTAVLTNGEETFSSIFAELEKAEHHIHLEYYIVRHDEIGQQLKRVLMEKARQG 192 Query: 181 VRVRLLCDGLGSHKLGRRFIRTLRNAGVEVHFFLPPLISLLDRRFNFRNHRKIIVVDGRV 240 VRVR L D +GS KL +I LR AGVE+ F P + L + NFRNHRKIIV+DG V Sbjct: 193 VRVRFLYDAVGSWKLSNAYIEELRAAGVEMIPFSPVRLPFLSNQINFRNHRKIIVIDGGV 252 Query: 241 GFTGGLNIGDDYLGKDPKLGYWRDTHLRLEGDAVYSLQYIFLKDWRLATGEGMNHPRFF- 299 GF GGLNIGD+YLGK+ G+WRDTHL + G+AV +LQ IFL+DW TGE + P + Sbjct: 253 GFVGGLNIGDEYLGKNKYFGFWRDTHLLIRGEAVRTLQLIFLQDWYYMTGERLLTPDYLS 312 Query: 300 -PAHKCTDQEAVLMVASGPDGALDASQEMYFASIVAAKQRIWITSPYFIPDPTICRALKN 358 P Q V ++A GPD + +++YFA I +AKQ IW+ SPYF+PD I ALK Sbjct: 313 PPLIVEEGQGGVQLIAGGPDQKWEVIKQLYFAMITSAKQSIWVASPYFVPDEDILTALKV 372 Query: 359 AVLSGVDVRIIIPAIPDNRLVYYASLSYLENLQDAGVKFYRYQKGFMHAKVMIIDDLLGT 418 A LSG+DVR++ P PD ++V+YAS SY L +AGVK Y Y+KGF+H+KV+++D L + Sbjct: 373 AALSGIDVRLLAPKRPDKKIVFYASRSYFPELLEAGVKIYEYEKGFLHSKVIVVDGELAS 432 Query: 419 VGSANLDMRSFYSNFELTAVLLRPEVIAGLAASVEQDLQHSEFIEPVIFRRRGNHVKLIE 478 +G+AN+DMRSF+ NFE+ A L + I L +D +H+ I+ F++R V++ E Sbjct: 433 IGTANMDMRSFHLNFEVNAFLYYTDSIHKLVRDFLEDFRHASMIDYEQFQQRPFRVRIAE 492 Query: 479 SLCQLLSPLL 488 S+ +LLSPLL Sbjct: 493 SVSRLLSPLL 502 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.144 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,804,373,697 Number of extensions: 113988434 Number of successful extensions: 294273 Number of sequences better than 1.0e-05: 2232 Number of HSP's gapped: 287369 Number of HSP's successfully gapped: 2333 Length of query: 488 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 348 Effective length of database: 1,741,754,103 Effective search space: 606130427844 Effective search space used: 606130427844 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)