BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0029.p (518 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KE53.1 B3KE53_9BACI 2-isopropylmalate synthase 783 0.0 B1DH41.1 B1DH41_9BACL 2-isopropylmalate synthase 634 e-180 C0Z635.1 C0Z635_BREBN Probable 2-isopropylmalate synthase EC=2.3... 632 e-179 Q2RG97.1 Q2RG97_MOOTA 2-isopropylmalate synthase EC=2.3.3.13 456 e-126 B9KB95.1 LEU1_THENN 2-isopropylmalate synthase EC=2.3.3.13 453 e-125 >B3KE53.1 B3KE53_9BACI 2-isopropylmalate synthase Length = 507 Score = 783 bits (2021), Expect = 0.0, Method: Compositional matrix adjust. Identities = 377/493 (76%), Positives = 426/493 (86%) Query: 6 KKRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETI 65 K+ I+IFDTTLRDGEQAPGASL PEQK++LA KLAELGVDV+EPGFPVSSPG+FAAV+ I Sbjct: 5 KRTIRIFDTTLRDGEQAPGASLQPEQKMVLAGKLAELGVDVMEPGFPVSSPGEFAAVQAI 64 Query: 66 SRKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAA 125 SR++Q VEICGFARAV+GDIDAAV+AT+DA RRR+HLFISSSDIHLRHQLRMSR EVVA Sbjct: 65 SRQLQQVEICGFARAVKGDIDAAVKATQDAARRRIHLFISSSDIHLRHQLRMSRPEVVAR 124 Query: 126 AREMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYG 185 AREMTAYARQF +VVEFTAMDA+RTGIDDLIEMVEAVIEEGATIINLPDTVGYALP EYG Sbjct: 125 AREMTAYARQFSDVVEFTAMDASRTGIDDLIEMVEAVIEEGATIINLPDTVGYALPNEYG 184 Query: 186 EMFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCAL 245 EMFRRVR+GARGG V+YSAHCHNDLGLAVANSLAAIE GA+QIEVTVNGVGERTGNCAL Sbjct: 185 EMFRRVREGARGGKRVAYSAHCHNDLGLAVANSLAAIENGASQIEVTVNGVGERTGNCAL 244 Query: 246 EELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIHQ 305 EELVMAL TRGD L ETGI +KLY+ SR++SGAMHFPIA+NKPVVGRNAFQHESGIHQ Sbjct: 245 EELVMALSTRGDMLRGETGITAEKLYEVSRLVSGAMHFPIAFNKPVVGRNAFQHESGIHQ 304 Query: 306 DGLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESFK 365 DGLLKDRSTYEIMDPE+LGIPRSMI+LGKHSGRHALKDR A+YG+ L E+++LY++FK Sbjct: 305 DGLLKDRSTYEIMDPEKLGIPRSMIVLGKHSGRHALKDRLARYGVRLEGAEMESLYDTFK 364 Query: 366 ETADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEETS 425 ETADRQK VSDD+LL+MVS T G+ A+VYEL E QV++G+ RVAAVT+R L E + Sbjct: 365 ETADRQKVVSDDQLLQMVSRTLGKPAQVYELAEAQVISGTGSSRVAAVTLRQLSEQTEAT 424 Query: 426 HTSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKFRGTAI 485 ++S +GP+ F LELHSL SGE+A AEAAV VE G FRGTA+ Sbjct: 425 YSSVADGPVQAVISAISQGISSDIRFGDLELHSLSSGEDAHAEAAVTVEKAGKIFRGTAV 484 Query: 486 HQDIVMAAGIAYI 498 HQDIVMAAG+AY+ Sbjct: 485 HQDIVMAAGLAYV 497 >B1DH41.1 B1DH41_9BACL 2-isopropylmalate synthase Length = 513 Score = 634 bits (1634), Expect = e-180, Method: Compositional matrix adjust. Identities = 301/484 (62%), Positives = 381/484 (78%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 K+IQIFDTTLRDGEQ+PGAS+ PE+KII+A +LA+LGVD++EPGF +SSPG+FAA++ IS Sbjct: 2 KKIQIFDTTLRDGEQSPGASIEPERKIIIARQLAKLGVDIIEPGFAISSPGEFAAIQQIS 61 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAAA 126 R++Q VEICGFARAV+ DIDAAV+AT+DA +RRLHLFISSSDIHL HQ+R +R+EVV A Sbjct: 62 RELQNVEICGFARAVKVDIDAAVKATQDAAKRRLHLFISSSDIHLSHQIRKTRSEVVQIA 121 Query: 127 REMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYGE 186 R+M +YA+QF + ++F AMD+ R+ D +IE+VEA IE GATIINLPDT+GYALP E + Sbjct: 122 RDMISYAKQFVDEIQFAAMDSTRSDRDFVIELVEAAIESGATIINLPDTLGYALPNEMVD 181 Query: 187 MFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCALE 246 +F VRQ ARGGD V YS H HNDLG+AVANSLAA++ GATQ+EVTVNGVGER GNC+LE Sbjct: 182 LFTSVRQQARGGDKVRYSTHNHNDLGMAVANSLAAVQAGATQVEVTVNGVGERAGNCSLE 241 Query: 247 ELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIHQD 306 EL M +ETR AL ET I ++Y TS ++S AMH+PIAYNKP+VGRNAFQHESGIHQD Sbjct: 242 ELAMLIETRKSALEIETNIEQSEIYTTSHMVSRAMHYPIAYNKPIVGRNAFQHESGIHQD 301 Query: 307 GLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESFKE 366 GLLKDRSTYEIMDPE +GI RSMIILG+HSGRHA+K R ++YGIA++ EL+ +Y+ FK+ Sbjct: 302 GLLKDRSTYEIMDPEAMGISRSMIILGRHSGRHAIKHRLSEYGIAVADNELNDVYDKFKQ 361 Query: 367 TADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEETSH 426 AD +K V+DDEL+R+ T + Y+L ++QVLAGS + R+A+VT+R + G E S+ Sbjct: 362 VADEKKIVTDDELIRIAGEATETQPDPYKLMDLQVLAGSQKSRIASVTIRDGEDGGERSY 421 Query: 427 TSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKFRGTAIH 486 + G GPL F LELHSL +GE+A EA V + EG +FRGT+IH Sbjct: 422 SGMGEGPLEAVIHCIQQAIPVAAQFEDLELHSLSTGEDAHGEAVVSIVIEGERFRGTSIH 481 Query: 487 QDIV 490 +DI+ Sbjct: 482 KDII 485 >C0Z635.1 C0Z635_BREBN Probable 2-isopropylmalate synthase EC=2.3.3.13 Length = 514 Score = 632 bits (1630), Expect = e-179, Method: Compositional matrix adjust. Identities = 306/492 (62%), Positives = 377/492 (76%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 KRI IFDTTLRDGEQAPGASL+PEQKI++A LA+LGVDV+EPGFP+SSPGDF AV+ IS Sbjct: 3 KRIHIFDTTLRDGEQAPGASLSPEQKIVIAKHLADLGVDVIEPGFPISSPGDFMAVQRIS 62 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAAA 126 ++V GVEICGFARAV+ DIDAAVRAT+ AERRRLH+F+SSS IHL QLR SR +V+ A Sbjct: 63 QEVHGVEICGFARAVKEDIDAAVRATQAAERRRLHMFLSSSSIHLDFQLRKSREQVIKIA 122 Query: 127 REMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYGE 186 REM AY +QF + EF+ MDA RTG + L EM+EA IEEGATIIN+PDTVGYALP+EYG Sbjct: 123 REMIAYGKQFVDRFEFSPMDATRTGDEFLFEMIEAAIEEGATIINIPDTVGYALPEEYGA 182 Query: 187 MFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCALE 246 MF RV + RG D V +SAHCHNDLGLAVANSLAAI G TQ+EVTVNGVGER GNC+LE Sbjct: 183 MFTRVMKNVRGSDKVEFSAHCHNDLGLAVANSLAAIRAGVTQVEVTVNGVGERAGNCSLE 242 Query: 247 ELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIHQD 306 ELVMA+ETR +AL AET I + +Y TS+ +S AM FPIA+NKP+VGRNAFQHE+GIHQD Sbjct: 243 ELVMAIETRKEALDAETRIRPEHIYTTSQKVSRAMSFPIAFNKPIVGRNAFQHEAGIHQD 302 Query: 307 GLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESFKE 366 GLLK+RSTYEIMDPE LG+PR+MIILGKHSGRHA+K R ++GI L+ +++ALY FKE Sbjct: 303 GLLKERSTYEIMDPESLGVPRNMIILGKHSGRHAIKHRVNEFGIQLTDEQMEALYVQFKE 362 Query: 367 TADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEETSH 426 AD+QK V D +LL +V T + + L QVL G++ R+A+ T+ + +GEE ++ Sbjct: 363 LADKQKVVQDHQLLELVGHTVNAVLEPFTLVNAQVLTGTSGHRMASCTIMNNVTGEEQAY 422 Query: 427 TSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKFRGTAIH 486 + TG GP+ FT +EL+SL SGE+A EA V V G+ F+G A H Sbjct: 423 SGTGEGPVEALVAGIKQALPFDVEFTDMELYSLSSGEHATGEAIVTVSANGSHFKGVAEH 482 Query: 487 QDIVMAAGIAYI 498 +D++MA A++ Sbjct: 483 RDVLMAVAQAFM 494 >Q2RG97.1 Q2RG97_MOOTA 2-isopropylmalate synthase EC=2.3.3.13 Length = 517 Score = 456 bits (1173), Expect = e-126, Method: Compositional matrix adjust. Identities = 228/493 (46%), Positives = 324/493 (65%), Gaps = 4/493 (0%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 KRI IFDTTLRDGEQ+PG SL ++K+ +A +LA LGVDV+E GFP++SPGDF AV ++ Sbjct: 6 KRIYIFDTTLRDGEQSPGVSLNIQEKLEIARQLARLGVDVIEAGFPIASPGDFEAVRAVA 65 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRDAERRRLHLFISSSDIHLRHQLRMSRAEVVAAA 126 R+V+G I G AR DID A A ++A++ R+H+FI++SDIHL+++LRMSR EV+ A Sbjct: 66 REVEGPVIAGLARINERDIDRAWEALQEAQKPRIHVFIATSDIHLKYKLRMSRQEVLEAV 125 Query: 127 REMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEYGE 186 R A A+ +C+ +EF+ DA R+ +D L +++ IE GAT++N+PDTVGYA P+E+G Sbjct: 126 RRGVARAKGYCQDIEFSPEDAVRSDLDFLCQVLATAIEAGATVLNIPDTVGYATPEEFGR 185 Query: 187 MFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCALE 246 + ++RQ G D V S HCHNDLGLAVANSLAAIE GA Q+E +NG+GER GN ALE Sbjct: 186 LISQIRQRVPGIDKVRISVHCHNDLGLAVANSLAAIENGALQVEGAINGIGERAGNAALE 245 Query: 247 ELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIHQD 306 E++MAL TR D G TGIV +++Y TS+++S P+ YNK +VG+NAF HE+GIHQD Sbjct: 246 EVIMALYTRRDYYGCRTGIVTEEIYRTSKLVSSLTGMPVQYNKAIVGKNAFTHEAGIHQD 305 Query: 307 GLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESFKE 366 G+LK+R+TYEIM+P +G+ ++ I LGKHSGRHAL+ R + G L+ ELD + FKE Sbjct: 306 GVLKERTTYEIMNPAMIGLVQNNIFLGKHSGRHALRSRLEELGFKLTEAELDKAFARFKE 365 Query: 367 TADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEE-TS 425 ADR+K +SD +L +V + + + L + + G+ RV ++ GEE Sbjct: 366 LADRKKEISDRDLEAIVEHEVKRIPEKFVLEHIHISTGN---RVVPTATIGIRVGEELKE 422 Query: 426 HTSTGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKFRGTAI 485 + G GP+ L+++ G++A E V +E++G F G I Sbjct: 423 EAACGEGPVDAAFKAIDKLTRIPVCLKSYNLNAVTGGKDAVGEVTVKIEYDGRVFIGRGI 482 Query: 486 HQDIVMAAGIAYI 498 D++ A+ AY+ Sbjct: 483 STDVLEASARAYL 495 >B9KB95.1 LEU1_THENN 2-isopropylmalate synthase EC=2.3.3.13 Length = 513 Score = 453 bits (1166), Expect = e-125, Method: Compositional matrix adjust. Identities = 232/495 (46%), Positives = 317/495 (64%), Gaps = 6/495 (1%) Query: 7 KRIQIFDTTLRDGEQAPGASLTPEQKIILAYKLAELGVDVLEPGFPVSSPGDFAAVETIS 66 +RI+IFDTTLRDGEQ+PGAS++ E+K+ +A L +LGVD++E GFPVSSP F AV+ ++ Sbjct: 2 RRIKIFDTTLRDGEQSPGASMSVEEKVEMALMLEDLGVDLIEAGFPVSSPVQFEAVKRVA 61 Query: 67 RKVQGVEICGFARAVRGDIDAAVRATRD--AERRRLHLFISSSDIHLRHQLRMSRAEVVA 124 VQ + G AR V DIDAA A +D ++R +H+FI++S IH +++LRM + E++ Sbjct: 62 EAVQRPIVVGLARCVEKDIDAAYEALKDRPKDKRMIHVFIATSPIHRKYKLRMEKEEILE 121 Query: 125 AAREMTAYARQFCEVVEFTAMDAARTGIDDLIEMVEAVIEEGATIINLPDTVGYALPQEY 184 R+ YARQF ++VEF+A DA+RT + LIE+ IE GAT IN+PDTVGYALP E+ Sbjct: 122 RVRKYVTYARQFFDLVEFSAEDASRTEVPFLIEVYRTAIESGATTINVPDTVGYALPDEF 181 Query: 185 GEMFRRVRQGARGGDTVSYSAHCHNDLGLAVANSLAAIEGGATQIEVTVNGVGERTGNCA 244 GE+ R +++G G + V S HCHNDLGLAVANS+AA++ GATQ+EVT+NG+GER GNCA Sbjct: 182 GELIRTLKEGVPGIENVDLSVHCHNDLGLAVANSIAAVQNGATQVEVTLNGIGERAGNCA 241 Query: 245 LEELVMALETRGDALGAETGIVLDKLYDTSRIISGAMHFPIAYNKPVVGRNAFQHESGIH 304 LEE VMAL+ R D L ETGI + +Y SR+++ A NKP+VG N F HESGIH Sbjct: 242 LEEFVMALKVRKDRLPYETGIRTELIYPASRLLTHITGLIPARNKPIVGENVFLHESGIH 301 Query: 305 QDGLLKDRSTYEIMDPERLGIPRSMIILGKHSGRHALKDRAAKYGIALSPGELDALYESF 364 QDG+LK R TYEIM P +G ++LG+HSG+HAL+ + YGI L ++E F Sbjct: 302 QDGVLKHRETYEIMKPSDIGRSSETLVLGRHSGKHALRKKLESYGIKLDDETFQKVFEKF 361 Query: 365 KETADRQKAVSDDELLRMVSTTTGQEAKVYELGEVQVLAGSAQRRVAAVTVRHLKSGEET 424 E ADR+K V DD+L +VS + Y L V G+ AAV L+ G E Sbjct: 362 TELADRKKEVYDDDLFSIVSEVLKEPLNGYRLVHFHVHTGNTLLPTAAVV---LQVGNEK 418 Query: 425 SHTS-TGNGPLXXXXXXXXXXXXXXXXFTGLELHSLGSGENAQAEAAVMVEWEGNKFRGT 483 + TGNGP+ + ++G+G+NAQ E + ++ +G + G Sbjct: 419 KEAAETGNGPVDAIFKAIDKALGLQPKLEEYIIQAVGTGKNAQGEVKLTLKIDGELYSGR 478 Query: 484 AIHQDIVMAAGIAYI 498 + DIV A+ IAYI Sbjct: 479 GVSTDIVEASAIAYI 493 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,723,408,028 Number of extensions: 109984822 Number of successful extensions: 316519 Number of sequences better than 1.0e-05: 3195 Number of HSP's gapped: 310443 Number of HSP's successfully gapped: 3198 Length of query: 518 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 378 Effective length of database: 1,741,754,103 Effective search space: 658383050934 Effective search space used: 658383050934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)