BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0024.p (215 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KE51.1 B3KE51_9BACI 5-formyltetrahydrofolate cyclo-ligase 114 6e-24 B1DH51.1 B1DH51_9BACL 5-formyltetrahydrofolate cyclo-ligase 103 8e-21 Q3AE50.1 Q3AE50_CARHZ 5-formyltetrahydrofolate cyclo-ligase fami... 102 2e-20 A4A119.1 A4A119_9PLAN 5-formyltetrahydrofolate cyclo-ligase 100 9e-20 C1T3X7.1 C1T3X7_DESBA 5-formyltetrahydrofolate cyclo-ligase 98 5e-19 >B3KE51.1 B3KE51_9BACI 5-formyltetrahydrofolate cyclo-ligase Length = 215 Score = 114 bits (285), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 73/178 (41%), Positives = 107/178 (60%), Gaps = 6/178 (3%) Query: 29 DERASMSARVCSHALDWLRKEKAASLLAYVPFRSELDCRPLIAGAWALGREVLLPRVIAE 88 +ERA S + C+ A + + + S++ YVPFRSELD RPLI AW G +V++PR + + Sbjct: 30 EERAKRSKQACAFAAEVMDRLHLQSMMIYVPFRSELDSRPLIEWAWKQGIQVIVPRSLTQ 89 Query: 89 AGTMSLHTVGSWEELAPGAYGILEPLLPEGNGQEREXXXXXXXXXXXGLAFDRQGGRLGY 148 +M L+ + +W+EL GAYGI E P+ ++ GLAFDR GGRLGY Sbjct: 90 DRSMELYKLQAWDELVSGAYGISE---PDPLRSQKWNHPGPDIVWVPGLAFDRMGGRLGY 146 Query: 149 GKGYYDRMQASLK---QESGFALKPPVWIGLAFSQQLVPEVPMDGHDAYMDMLITEEG 203 G GYYDR+ L Q +K P+W+G+ + Q++ +VPM+ HD +D L+TE+G Sbjct: 147 GGGYYDRLSDRLNQGAQADARRIKKPLWVGIGYEVQVLEQVPMEEHDRVLDGLVTEQG 204 >B1DH51.1 B1DH51_9BACL 5-formyltetrahydrofolate cyclo-ligase Length = 212 Score = 103 bits (258), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 71/179 (39%), Positives = 96/179 (53%), Gaps = 18/179 (10%) Query: 29 DERASMSARVCSHALDWLRKEKAASLLAYVPFRSELDCRPLIAGAWALGREVLLPRVIAE 88 +ER S C + +W+ + AS + YVPFRSEL+ R LI W G EV++P+ Sbjct: 37 EERIIRSEAACRYLTNWMMEHGVASFMCYVPFRSELNTRSLIEWGWKTGAEVVVPKCYPA 96 Query: 89 AGTMSLHTVGSWEELAPGAYGILE------PLLPEGNGQEREXXXXXXXXXXXGLAFDRQ 142 +M+LH + +W L GAYGILE P LP+ G+AFD++ Sbjct: 97 DRSMTLHKLDNWSGLKAGAYGILEPDPDRCPALPDS--------YVPGLVVAPGIAFDKK 148 Query: 143 GGRLGYGKGYYDRMQASLKQESGFALKPPVWIGLAFSQQLVPEVPMDGHDAYMDMLITE 201 GGRLGYG GYYDR L +E W+G+AF QLV ++PM HD MD ++TE Sbjct: 149 GGRLGYGGGYYDRFAERLGRERADV----TWLGIAFEDQLVDDIPMQTHDRRMDGIVTE 203 >Q3AE50.1 Q3AE50_CARHZ 5-formyltetrahydrofolate cyclo-ligase family protein Length = 192 Score = 102 bits (254), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 63/180 (35%), Positives = 100/180 (55%), Gaps = 12/180 (6%) Query: 30 ERASMSARVCSHALDWLRKEKAASLLAYVPFRSELDCRPLIAGAWALGREVLLP-RVIAE 88 E A+ S+R+ + + ++A +++ Y+ FR E+ R +I WA G++V++P V++E Sbjct: 22 EIAAKSSRILTLLKAFAPYQQARTVMVYLDFRGEVKTREIIRDLWAYGKKVVIPVTVLSE 81 Query: 89 AGTMSLHTVGSWEELAPGAYGILEPLLPEGNGQEREXXXXXXXXXXXGLAFDRQGGRLGY 148 + ++ + + +L G YGILEP E E G+AFD +G RLGY Sbjct: 82 KKLLPVY-LRDFGDLVEGTYGILEPKEEARTIAEPE---VIDLVLVPGVAFDLKGNRLGY 137 Query: 149 GKGYYDRMQASLKQESGFALKPPVWIGLAFSQQLVPEVPMDGHDAYMDMLITEEGIFHCR 208 G G+YDR +L+ A+K + LAF Q+VP++P HD MD +ITEE I +CR Sbjct: 138 GAGFYDRFLPNLQP----AVKK---VALAFELQIVPKIPTGPHDIPMDYIITEERIINCR 190 >A4A119.1 A4A119_9PLAN 5-formyltetrahydrofolate cyclo-ligase Length = 197 Score = 100 bits (249), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 60/182 (32%), Positives = 95/182 (52%), Gaps = 9/182 (4%) Query: 30 ERASMSARVCSHALDWLRKEKAASLLAYVPFRSELDCRPLIAGAWALGREVLLPRVIAEA 89 +R+ S R+ + +WL ++ AA+LL YV R E+D R L+ A R V++P + + Sbjct: 25 DRSERSVRIQARISNWLPQQSAATLLCYVNARCEVDTRSLLHQLLADERRVVVPYCLDD- 83 Query: 90 GTMSLHTVGSWEELAPGAYGILEPLLPEGNGQEREXXXXXXXXXXXGLAFDRQGGRLGYG 149 + L + + ELA G +GILEP + +R G+AFD G RLGYG Sbjct: 84 NHLGLFLLSNMSELATGRFGILEPR-HQLRDSKRISPVEIDLAILPGVAFDLHGNRLGYG 142 Query: 150 KGYYDRMQASLKQESGFALKPPVWIGLAFSQQLVPEVPMDGHDAYMDMLITEEGIFHCRK 209 KGY+DR+ + ++ ++ V LAF QL ++P + HD +D L+TE+ + Sbjct: 143 KGYFDRLLSEMRPQA-------VTCALAFECQLTAQIPTEPHDLPIDYLVTEDRLIRSAA 195 Query: 210 EN 211 N Sbjct: 196 AN 197 >C1T3X7.1 C1T3X7_DESBA 5-formyltetrahydrofolate cyclo-ligase Length = 196 Score = 98.2 bits (243), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 64/183 (34%), Positives = 97/183 (53%), Gaps = 23/183 (12%) Query: 35 SARVCSHALDWLRKEKAASLLAYVPFRSELDCRPLIAGAWALGREVLLPRVI-AEAGTMS 93 SAR+ + L R A S++ Y+P R E+D PL+ WA G E+LLPR G M Sbjct: 29 SARIRDNLLSLERIRAACSVMLYLPARGEVDTWPLLDHFWARGSEILLPRCCDGSPGIMD 88 Query: 94 LHTVGSWEELAPGAYGILEP------LLPEGNGQEREXXXXXXXXXXXGLAFDRQGGRLG 147 ++ V S E+L PG +G++EP L+P + LAFD++G RLG Sbjct: 89 VYAVTSREDLGPGCFGLIEPRAQRALLVPAPQPE---------VIIVPALAFDQRGYRLG 139 Query: 148 YGKGYYDRMQASLKQESGFALKPPVWIGLAFSQQLVPEVPMDGHDAYMDMLITEEGIFHC 207 +G GYYDR +L G A P+ +G A++ Q++ +P++ D ++M++T E I H Sbjct: 140 FGGGYYDRFLPTL----GCA---PLLVGPAYAFQVLESLPVEPWDRPVEMVVTPESILHI 192 Query: 208 RKE 210 E Sbjct: 193 PAE 195 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,161,700,829 Number of extensions: 44456193 Number of successful extensions: 79787 Number of sequences better than 1.0e-05: 694 Number of HSP's gapped: 79346 Number of HSP's successfully gapped: 694 Length of query: 215 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 85 Effective length of database: 1,835,964,253 Effective search space: 156056961505 Effective search space used: 156056961505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)