BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0020.p (257 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KE47.1 B3KE47_9BACI Sec-independent protein translocase, TatC ... 347 6e-94 B1DH56.1 B1DH56_9BACL Sec-independent protein translocase, TatC ... 282 2e-74 C0ZK48.1 C0ZK48_BREBN Sec-independent protein translocase protein 234 7e-60 B1D667.1 B1D667_9BACL Sec-independent protein translocase, TatC ... 227 6e-58 B3K4Q9.1 B3K4Q9_9BACI Sec-independent protein translocase, TatC ... 211 4e-53 >B3KE47.1 B3KE47_9BACI Sec-independent protein translocase, TatC subunit Length = 257 Score = 347 bits (890), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 172/256 (67%), Positives = 201/256 (78%) Query: 1 MSLEAEEMSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAF 60 MS + ++M++V+HL ELR+RII PIY YL A+ A+ F LHAF Sbjct: 1 MSEQVKDMALVEHLGELRKRIIIVLVVFVAGLVGGLFVADPIYHYLTAAEEAKNFELHAF 60 Query: 61 SFWDGIGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYVPYVFVLFILGI 120 SFWDGIG+Y+KIA SL +SVP I YQLWAF+ PGLR E+ AA+RYVPY FVLFI+GI Sbjct: 61 SFWDGIGIYMKIAAIFSLVISVPLIFYQLWAFVKPGLRKEEQKAAIRYVPYAFVLFIVGI 120 Query: 121 AFAYYIVFPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTKL 180 F YYIVFPM LSFT IT+ MGL+ETYGI Y +FMF+LV+PLALLFELPLVVMFLT++ Sbjct: 121 TFGYYIVFPMTLSFTTAITQGMGLQETYGITNYLNFMFNLVLPLALLFELPLVVMFLTRI 180 Query: 181 RILNPLRLRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYRKQL 240 RILNPLRLRKMR+ AYFALVFIAVVITPPDFISDFLVTIPLL+LYEFSVFLSS +YRKQL Sbjct: 181 RILNPLRLRKMRKVAYFALVFIAVVITPPDFISDFLVTIPLLLLYEFSVFLSSVIYRKQL 240 Query: 241 AMDRELEARYVRKEES 256 D E ++RYVR +E Sbjct: 241 EADMERDSRYVRADEE 256 >B1DH56.1 B1DH56_9BACL Sec-independent protein translocase, TatC subunit Length = 270 Score = 282 bits (722), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 130/251 (51%), Positives = 182/251 (72%) Query: 4 EAEEMSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAFSFW 63 E M + +HL ELR+RIIY +P Y +L+ + A+G LHAFS W Sbjct: 20 EEGLMPLFEHLGELRKRIIYMLIVIVAGLIIGLVIAEPAYNFLMEQEPAKGLQLHAFSLW 79 Query: 64 DGIGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYVPYVFVLFILGIAFA 123 DGIG+Y+K A ++L +++PF YQLWAF+ P L+P E+ A ++Y+P+ ++F++G+AF+ Sbjct: 80 DGIGIYMKFAFVIALLLAIPFTAYQLWAFVKPALKPNEQRATIKYIPFALIMFLIGLAFS 139 Query: 124 YYIVFPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTKLRIL 183 Y++VFP+A FT ++ ++ LEET GI QYFSFMF+++IP++LLFELPLV+MFLT +RIL Sbjct: 140 YFVVFPLAFHFTRKVSANLNLEETIGITQYFSFMFNILIPISLLFELPLVIMFLTAIRIL 199 Query: 184 NPLRLRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYRKQLAMD 243 NP+RLRKMRR AYF ++FI VV+TPPDF+SD LV IPL+ILYE SV LS VY+KQLA Sbjct: 200 NPVRLRKMRRIAYFLMIFIGVVVTPPDFVSDSLVAIPLIILYEISVLLSGTVYKKQLAAA 259 Query: 244 RELEARYVRKE 254 + EA + + Sbjct: 260 EKREAEWAAGQ 270 >C0ZK48.1 C0ZK48_BREBN Sec-independent protein translocase protein Length = 253 Score = 234 bits (596), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 114/250 (45%), Positives = 159/250 (63%) Query: 8 MSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAFSFWDGIG 67 M++V+HLTELR+RII+ P+ YL +A G + + D + Sbjct: 1 MTLVEHLTELRKRIIWVLAVFIVALIVGFFLAGPLVDYLKQEPIADGVPIVSLHPSDALR 60 Query: 68 MYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYVPYVFVLFILGIAFAYYIV 127 +Y+++A V +++P +Y +W F+SPGL E+ L ++P LFI+G+ F YY+V Sbjct: 61 VYMQVAFLVGAVITLPVALYHVWRFVSPGLEEQEKRGTLYFIPAACFLFIIGVLFGYYVV 120 Query: 128 FPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTKLRILNPLR 187 FPM + F IT SMG + YGIAQYF FMF++VIP +LF+LP++VMFLT+LRILNP+R Sbjct: 121 FPMVMQFMTGITASMGADPNYGIAQYFGFMFNMVIPFGILFQLPIIVMFLTRLRILNPMR 180 Query: 188 LRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYRKQLAMDRELE 247 L K RRYAYFAL + + +TPP+ +SD LVTIPLL+LYE S++LS VYRKQL E Sbjct: 181 LAKARRYAYFALAVVGITLTPPEIVSDILVTIPLLLLYELSIWLSRIVYRKQLKEQEAWE 240 Query: 248 ARYVRKEESR 257 Y E + Sbjct: 241 QEYGSLETNE 250 >B1D667.1 B1D667_9BACL Sec-independent protein translocase, TatC subunit Length = 250 Score = 227 bits (579), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 121/236 (51%), Positives = 158/236 (66%) Query: 6 EEMSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAFSFWDG 65 E M+++ HLTELR RI + YL + FS WDG Sbjct: 6 ETMTLLGHLTELRSRIWKILIVVLLSLGGGLYLSPKVLRYLKSVPPGSEMTWNVFSPWDG 65 Query: 66 IGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYVPYVFVLFILGIAFAYY 125 I MY+ +A+ ++LAVS+PFI+YQLW F+ GL P ER+AALRYVPY + F G+AF Y+ Sbjct: 66 IRMYMSVALVIALAVSLPFILYQLWGFVKQGLHPGERNAALRYVPYSVICFFTGLAFGYF 125 Query: 126 IVFPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTKLRILNP 185 +VFPM+ SFT IT+SM L ETYG+AQYF FMF++V+PLA+ FELP++VMFLTK+ +L P Sbjct: 126 VVFPMSFSFTSKITKSMELVETYGVAQYFGFMFNIVLPLAIAFELPVIVMFLTKIGVLTP 185 Query: 186 LRLRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYRKQLA 241 L KMRRYAY LV +A +I+PP+ IS LV +PL++LYE SV LS VY K+ A Sbjct: 186 KALHKMRRYAYLVLVIVASLISPPELISHLLVFVPLVVLYEISVLLSRIVYGKRKA 241 >B3K4Q9.1 B3K4Q9_9BACI Sec-independent protein translocase, TatC subunit Length = 256 Score = 211 bits (538), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 100/240 (41%), Positives = 158/240 (65%) Query: 6 EEMSVVDHLTELRRRIIYXXXXXXXXXXXXXXXXKPIYLYLIHADMAQGFVLHAFSFWDG 65 M++++H+ ELR+RI+ + L H A + FS WDG Sbjct: 5 NTMTLLEHIAELRKRILLVAAVFLLSMAAGLFAAPHVLSMLKHHSPAFQVTWNVFSPWDG 64 Query: 66 IGMYVKIAMAVSLAVSVPFIVYQLWAFISPGLRPVERSAALRYVPYVFVLFILGIAFAYY 125 + +Y+ I++ +SL ++PF ++Q+W F++ GL E+ AALR++P+ + F++G+AFAY+ Sbjct: 65 LRIYMSISIVLSLLPTLPFALHQIWLFVNQGLNGREQKAALRFIPFSALCFLIGVAFAYF 124 Query: 126 IVFPMALSFTITITRSMGLEETYGIAQYFSFMFSLVIPLALLFELPLVVMFLTKLRILNP 185 +VFPM+L+FT I M L ETYG+AQYF FMF+++IPL+L FELP++V+FLT++ IL+P Sbjct: 125 VVFPMSLAFTTGINSQMDLVETYGVAQYFGFMFNIIIPLSLAFELPVIVLFLTRIGILSP 184 Query: 186 LRLRKMRRYAYFALVFIAVVITPPDFISDFLVTIPLLILYEFSVFLSSFVYRKQLAMDRE 245 L MRRYAY LV +A +I+PPD +S +V +PL+ LYE S +L+ +++RK + E Sbjct: 185 AILHTMRRYAYVVLVIVAALISPPDLVSHLMVAVPLIGLYEISTWLAGWMHRKGKHAEHE 244 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.335 0.147 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,264,074,101 Number of extensions: 47061298 Number of successful extensions: 235281 Number of sequences better than 1.0e-05: 1304 Number of HSP's gapped: 232876 Number of HSP's successfully gapped: 1308 Length of query: 257 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 124 Effective length of database: 1,807,701,208 Effective search space: 224154949792 Effective search space used: 224154949792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 130 (54.7 bits)