BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0017.p (433 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DDI7.1 B1DDI7_9BACL Ammonium transporter 437 e-121 C1TA41.1 C1TA41_9FIRM Ammonium transporter 354 1e-95 C3J707.1 C3J707_9BACI Ammonium transporter 333 3e-89 B7Y6N8.1 B7Y6N8_9BACI Ammonium transporter 333 3e-89 A4XMB9.1 A4XMB9_CALS8 Ammonium transporter 314 1e-83 >B1DDI7.1 B1DDI7_9BACL Ammonium transporter Length = 430 Score = 437 bits (1124), Expect = e-121, Method: Compositional matrix adjust. Identities = 258/423 (60%), Positives = 310/423 (73%), Gaps = 12/423 (2%) Query: 7 SLGGGVDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLIFYIFG 66 L G+DTIWVVL+AAMILLMEGGFALLEAGFVR KN+V+IIMKVF DI G L+FY FG Sbjct: 5 ELSRGLDTIWVVLTAAMILLMEGGFALLEAGFVRQKNAVSIIMKVFVDIVFGALVFYFFG 64 Query: 67 FGLMYGKDAGGLVGTSGFMLGGDLSNLHFTISVDTFWLFQAAFTIAVISIVSGAVAERIN 126 F +MYGKD GGL GTSGFML GDL++++ IS +T+WLFQ AF IAVISIVSGAVAERI+ Sbjct: 65 FAIMYGKDIGGLFGTSGFMLNGDLTHINLNISHETYWLFQCAFVIAVISIVSGAVAERIH 124 Query: 127 FRAYLLYVIFMTGLIYPLX-XXXXXXXXXXXXXXXXDFAGSAVIHALGGFSALAAAIVIG 185 FRAY+L I MTGLIYPL DFAGSA IHALGGF+ALAA + IG Sbjct: 125 FRAYILLTIAMTGLIYPLSGHWVWAVNGWLGNLGMIDFAGSAAIHALGGFAALAATLFIG 184 Query: 186 PRKGKFNQAGNSTVALPSNLPLASVXXXXXXXXXXXXXXXSTLSATDARIGHIAITTMLS 245 PR GKF+ G + PSNLPLASV STLSAT+A IGHIAITTMLS Sbjct: 185 PRIGKFSPDGKVNIVSPSNLPLASVGAFILWFGWFGFNAGSTLSATNAAIGHIAITTMLS 244 Query: 246 AAAGGGITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAILIGAVSGLLMMA 305 AA+GG I +++T+FRY+++D P VINGSLAGLVGITAGCAFVSDGAAI IG++ G+ M+ Sbjct: 245 AASGGAICILFTMFRYRKADPPMVINGSLAGLVGITAGCAFVSDGAAIWIGSICGIAMVL 304 Query: 306 ATNWLEARRIDDPVGAFPVHAVSGAWGTVAVGLFA----TDG------GLLYGGGWHQLG 355 T ++E ++IDDPVGAF VH +SG+ GT+AVGL A T+G GLLYGGGW+ LG Sbjct: 305 VTEFMERKQIDDPVGAFAVHGISGSIGTLAVGLCAIPELTEGVGNGYYGLLYGGGWNLLG 364 Query: 356 VQILGLVTLAAWGFATTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGMIAAHRSAEFIEL 415 Q LGLV +A WGF TW L LI K++ VR S++EE +GLD+ HG+ A + +FI++ Sbjct: 365 AQALGLVVIAIWGFGLTWAVLWLIRKVMPVRVSKDEELVGLDVGIHGVPAYSQENDFIDV 424 Query: 416 EDH 418 DH Sbjct: 425 -DH 426 >C1TA41.1 C1TA41_9FIRM Ammonium transporter Length = 445 Score = 354 bits (908), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 189/406 (46%), Positives = 265/406 (65%), Gaps = 1/406 (0%) Query: 1 MNVTLESLGGGVDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTL 60 M V+ +SL G+DT+WV+L AA++ LMEGGFA LEAGF+R KNSVNI+MKVF D T+G L Sbjct: 1 MEVSSQSLAVGLDTVWVLLCAALVFLMEGGFAFLEAGFIRAKNSVNIVMKVFTDTTVGML 60 Query: 61 IFYIFGFGLMYGKDAGGLVGTSGFMLGGDLSNLHFTISVDTFWLFQAAFTIAVISIVSGA 120 +++ GF +M+G D GL G+ G+M+ G S+L I + FWLFQAAF +A+ SIVSGA Sbjct: 61 AYWLIGFSVMFGLDHSGLWGSGGYMVSGSFSHLGLRIPICAFWLFQAAFAMALASIVSGA 120 Query: 121 VAERINFRAYLLYVIFMTGLIYPLXXXXXXXXXX-XXXXXXXDFAGSAVIHALGGFSALA 179 VAER+ F Y++Y +YP+ DFAGSAV+HA+GG+SALA Sbjct: 121 VAERMKFFPYMVYTFCAAAFVYPIAGHWVWNSGGWLAQMGMKDFAGSAVVHAVGGWSALA 180 Query: 180 AAIVIGPRKGKFNQAGNSTVALPSNLPLASVXXXXXXXXXXXXXXXSTLSATDARIGHIA 239 A +V+GPR+GKFN+ G+ V NL LA + S+LS D I IA Sbjct: 181 AVMVLGPRRGKFNEDGSVNVIPAHNLHLAFLGAFILWFGWFGFNPGSSLSGLDGNIASIA 240 Query: 240 ITTMLSAAAGGGITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAILIGAVS 299 +TT LSAAAGG + +++T +Y ++D +NG+LAGLV IT G A+V+ G++++IG ++ Sbjct: 241 MTTNLSAAAGGFMGMVFTRLKYGKADPSMAMNGALAGLVAITGGTAYVTPGSSVIIGLIA 300 Query: 300 GLLMMAATNWLEARRIDDPVGAFPVHAVSGAWGTVAVGLFATDGGLLYGGGWHQLGVQIL 359 G+L++ A + + + DDPVGA VH V+G WG +A+GLFA DGGL YGGG L +Q L Sbjct: 301 GILVVLAVEFFDKVKADDPVGAIAVHGVNGVWGALALGLFAKDGGLFYGGGVKFLSIQAL 360 Query: 360 GLVTLAAWGFATTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGMIA 405 G+V+++ W F T+ L+ V +R + +EE GLD+S HG+ A Sbjct: 361 GVVSVSVWAFGLTFLIFSLLKATVGIRVTADEEMEGLDLSEHGIPA 406 >C3J707.1 C3J707_9BACI Ammonium transporter Length = 433 Score = 333 bits (853), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 189/406 (46%), Positives = 257/406 (63%), Gaps = 2/406 (0%) Query: 12 VDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLIFYIFGFGLMY 71 V+ +W+V++AAM+L MEGGF+LLEAG VR KN+VN+ MK+F D+T+G L F+ GFGLM+ Sbjct: 5 VNAVWIVIAAAMVLFMEGGFSLLEAGLVRTKNAVNVTMKIFVDLTVGALAFWFIGFGLMF 64 Query: 72 GKDAGGLVGTSGFMLGGDLSNLHFTISVDTFWLFQAAFTIAVISIVSGAVAERINFRAYL 131 G DA G VGT+ F +S L + F LFQ F +A +SI+SGAVAER+NF+AY+ Sbjct: 65 GDDAFGWVGTTLFGTPERIS-LSVPLPSAAFVLFQIGFAVACVSIISGAVAERMNFKAYI 123 Query: 132 LYVIFMTGLIYPLXXXXXXXXXX-XXXXXXXDFAGSAVIHALGGFSALAAAIVIGPRKGK 190 + V+ + +YPL DFAGSA IHALGGF+ALA A +GPRKG+ Sbjct: 124 VTVLLVCAAVYPLSGHWIWHSDGWLAKLGMKDFAGSAAIHALGGFAALAIAKRLGPRKGR 183 Query: 191 FNQAGNSTVALPSNLPLASVXXXXXXXXXXXXXXXSTLSATDARIGHIAITTMLSAAAGG 250 FN G+ V PSN+PLAS STL A++ + IA+ TMLS AAGG Sbjct: 184 FNSDGSVNVFAPSNIPLASAGAFILWFGWFAFNAGSTLDASNKALASIALNTMLSGAAGG 243 Query: 251 GITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAILIGAVSGLLMMAATNWL 310 L T+ +Y ++D V+NG L+GLV ITAGCAFVS +A+LIG VSG++++ AT + Sbjct: 244 ASALFVTMKKYGKADPSMVMNGVLSGLVAITAGCAFVSQWSAVLIGLVSGVIVVYATLLV 303 Query: 311 EARRIDDPVGAFPVHAVSGAWGTVAVGLFATDGGLLYGGGWHQLGVQILGLVTLAAWGFA 370 +A +IDDPVGA VH +G +GT+AVGLF + GLL G Q+LG +T+ WGF Sbjct: 304 DALKIDDPVGAVAVHGFNGVFGTLAVGLFDSTQGLLTTGHAWLFTTQLLGALTVIVWGFV 363 Query: 371 TTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGMIAAHRSAEFIELE 416 + + V +R++E EEE GLD++YHG+ A + F +L Sbjct: 364 SGALIANVCDSTVGLRATEREEEEGLDMAYHGIPAYNELERFADLP 409 >B7Y6N8.1 B7Y6N8_9BACI Ammonium transporter Length = 433 Score = 333 bits (853), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 189/406 (46%), Positives = 257/406 (63%), Gaps = 2/406 (0%) Query: 12 VDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLIFYIFGFGLMY 71 V+ +W+V++AAM+L MEGGF+LLEAG VR KN+VN+ MK+F D+T+G L F+ GFGLM+ Sbjct: 5 VNAVWIVIAAAMVLFMEGGFSLLEAGLVRTKNAVNVTMKIFVDLTVGALAFWFIGFGLMF 64 Query: 72 GKDAGGLVGTSGFMLGGDLSNLHFTISVDTFWLFQAAFTIAVISIVSGAVAERINFRAYL 131 G DA G VGT+ F +S L + F LFQ F +A +SI+SGAVAER+NF+AY+ Sbjct: 65 GDDAFGWVGTTLFGTPERIS-LSVPLPSAAFVLFQIGFAVACVSIISGAVAERMNFKAYI 123 Query: 132 LYVIFMTGLIYPLXXXXXXXXXX-XXXXXXXDFAGSAVIHALGGFSALAAAIVIGPRKGK 190 + V+ + +YPL DFAGSA IHALGGF+ALA A +GPRKG+ Sbjct: 124 VTVLLVCAAVYPLSGHWIWHSDGWLAKLGMKDFAGSAAIHALGGFAALAIAKRLGPRKGR 183 Query: 191 FNQAGNSTVALPSNLPLASVXXXXXXXXXXXXXXXSTLSATDARIGHIAITTMLSAAAGG 250 FN G+ V PSN+PLAS STL A++ + IA+ TMLS AAGG Sbjct: 184 FNSDGSVNVFAPSNIPLASAGAFILWFGWFAFNAGSTLDASNKALASIALNTMLSGAAGG 243 Query: 251 GITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAILIGAVSGLLMMAATNWL 310 L T+ +Y ++D V+NG L+GLV ITAGCAFVS +A+LIG VSG++++ AT + Sbjct: 244 ASALFVTMKKYGKADPSMVMNGVLSGLVAITAGCAFVSQWSAVLIGLVSGVIVVYATLLV 303 Query: 311 EARRIDDPVGAFPVHAVSGAWGTVAVGLFATDGGLLYGGGWHQLGVQILGLVTLAAWGFA 370 +A +IDDPVGA VH +G +GT+AVGLF + GLL G Q+LG +T+ WGF Sbjct: 304 DALKIDDPVGAVAVHGFNGVFGTLAVGLFDSTQGLLTTGHAWLFTTQLLGALTVIVWGFV 363 Query: 371 TTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGMIAAHRSAEFIELE 416 + + V +R++E EEE GLD++YHG+ A + F +L Sbjct: 364 SGALIANVCDSTVGLRATEREEEEGLDMAYHGIPAYNELERFADLP 409 >A4XMB9.1 A4XMB9_CALS8 Ammonium transporter Length = 445 Score = 314 bits (805), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 171/405 (42%), Positives = 248/405 (61%), Gaps = 1/405 (0%) Query: 2 NVTLESLGGGVDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLI 61 +T E + +D +WV+++A ++ M+ GFA++EAGF R KN+ NI+MK D IG++I Sbjct: 29 QITPEKVATAIDNVWVLITAFLVFFMQAGFAMVEAGFTRAKNASNIVMKNLMDFAIGSVI 88 Query: 62 FYIFGFGLMYGKDAGGLVGTSGFMLGGDLSNLHFTISVDTFWLFQAAFTIAVISIVSGAV 121 F++FGF M+GKDAGG +GTSGF L +L +I + +F +FQ F +IVSGA+ Sbjct: 89 FWLFGFAFMFGKDAGGFIGTSGFFLSDSFKHLGLSIPLTSFLMFQTVFAATAATIVSGAM 148 Query: 122 AERINFRAYLLYVIFMTGLIYPLXXXXXXXXXXXXXXXXXDFAGSAVIHALGGFSALAAA 181 AER F AY +Y ++ +IYP+ DFAGS V+H++GG+SAL A Sbjct: 149 AERTKFIAYCIYSAVISFIIYPVVGHWAWGGGWLSKLGFIDFAGSTVVHSVGGWSALIGA 208 Query: 182 IVIGPRKGKFNQAGNSTVALPSNLPLASVXXXXXXXXXXXXXXXSTLSATDARIGHIAIT 241 ++GPR GK+ + G ++ LA++ STLS+ + +IG IA+ Sbjct: 209 AMLGPRIGKYTKDGKVNAIPGHSITLAALGTFILWFGWFGFNPGSTLSSMNDKIGDIAVN 268 Query: 242 TMLSAAAGGGITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAILIGAVSGL 301 T L+AA G + +IYT +YK+ D +NG+LAGLV ITAGCA V+ A +IG ++G+ Sbjct: 269 TNLAAAMGANLAMIYTWLKYKKPDVSMTLNGALAGLVAITAGCASVNAWGAAIIGGLAGI 328 Query: 302 LMMAATNWLEAR-RIDDPVGAFPVHAVSGAWGTVAVGLFATDGGLLYGGGWHQLGVQILG 360 L++ A +++ + +IDDPVGA VH V GA+GT+ VGLFATDGGL YGGG Q VQ+ G Sbjct: 329 LVVVAVEFIDKKLKIDDPVGAISVHGVCGAFGTLMVGLFATDGGLFYGGGIKQFLVQLAG 388 Query: 361 LVTLAAWGFATTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGMIA 405 + + W T + +I V +R SEEEE GLD++ HG A Sbjct: 389 VASTFIWTTITAFILFAIIKLTVGLRVSEEEEIEGLDVTEHGTAA 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.140 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,302,348,165 Number of extensions: 92870880 Number of successful extensions: 268135 Number of sequences better than 1.0e-05: 2159 Number of HSP's gapped: 259590 Number of HSP's successfully gapped: 2297 Length of query: 433 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 294 Effective length of database: 1,751,175,118 Effective search space: 514845484692 Effective search space used: 514845484692 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)