BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0025.p (257 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9AKT4.1 NIFH2_PAEAZ Nitrogenase iron protein 2 EC=1.18.6.1 421 e-116 Q24Q92.1 Q24Q92_DESHY Nitrogenase iron protein EC=1.18.6.1 382 e-104 B8G291.1 B8G291_DESHD Nitrogenase iron protein EC=1.18.6.1 382 e-104 B9M031.1 B9M031_GEOSF Nitrogenase iron protein EC=1.18.6.1 372 e-101 Q2RW96.1 Q2RW96_RHORT Nitrogenase iron protein EC=1.18.6.1 369 e-100 >Q9AKT4.1 NIFH2_PAEAZ Nitrogenase iron protein 2 EC=1.18.6.1 Length = 292 Score = 421 bits (1081), Expect = e-116, Method: Compositional matrix adjust. Identities = 200/257 (77%), Positives = 224/257 (87%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDNHAVRAQDVIFEGFNGIYXXXXXXXXXXX 60 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRD VRA DVIFEGFNGIY Sbjct: 36 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDKQIVRAHDVIFEGFNGIYCVEAGGPAPGV 95 Query: 61 XXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVTSAD 120 IITSVSLLKQQ++FEELDLD+VIYDVLGDVVCGGFAVPVREGIAEHV+TVTSAD Sbjct: 96 GCAGRGIITSVSLLKQQKVFEELDLDYVIYDVLGDVVCGGFAVPVREGIAEHVFTVTSAD 155 Query: 121 FMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYVPRS 180 FMA+YAANNLFKGI KYS +GGALLGGVIANSINAPYAK+IVDDFV RT TQV+EYVPRS Sbjct: 156 FMALYAANNLFKGIHKYSTEGGALLGGVIANSINAPYAKEIVDDFVARTHTQVMEYVPRS 215 Query: 181 VTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLDTQELRQWAAEWGKQ 240 V+VTQ+ELQGKTTIEA P+S+QA++Y++LA+KI DHT SKVP PL+T ELR+WA+ WGKQ Sbjct: 216 VSVTQAELQGKTTIEADPNSKQAQIYKSLAQKIVDHTESKVPVPLETSELREWASNWGKQ 275 Query: 241 LVALESGLVAPAAVGNL 257 LV LE+G+++PAA GNL Sbjct: 276 LVELEAGVLSPAAAGNL 292 >Q24Q92.1 Q24Q92_DESHY Nitrogenase iron protein EC=1.18.6.1 Length = 292 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 180/249 (72%), Positives = 206/249 (82%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDNHAVRAQDVIFEGFNGIYXXXXXXXXXXX 60 VMQFGCDPKSDSTNTLRGG+YIPTVLDTLR+ V+A +VIFEGFNGIY Sbjct: 36 VMQFGCDPKSDSTNTLRGGKYIPTVLDTLREKSVVKASEVIFEGFNGIYCVEAGGPAPGV 95 Query: 61 XXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVTSAD 120 IIT+V L KQQ++F LDLDFVIYDVLGDVVCGGFA+P+REGIAEHV+TV+S+D Sbjct: 96 GCAGRGIITAVELFKQQKVFASLDLDFVIYDVLGDVVCGGFAIPIREGIAEHVFTVSSSD 155 Query: 121 FMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYVPRS 180 FM+IYA+NNLFKGI+KYS GGALLGGVIANSIN YAK IVDDFV+RT TQVVEY+PRS Sbjct: 156 FMSIYASNNLFKGIKKYSNSGGALLGGVIANSINQGYAKAIVDDFVERTKTQVVEYIPRS 215 Query: 181 VTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLDTQELRQWAAEWGKQ 240 +TVTQSELQGKTTIEAAP+SEQAKVY LA KI +H SKVPAPL+ +EL++WA W Q Sbjct: 216 ITVTQSELQGKTTIEAAPESEQAKVYTRLAEKIVEHKSSKVPAPLEIKELQEWATSWSDQ 275 Query: 241 LVALESGLV 249 L+ALE G+V Sbjct: 276 LLALERGVV 284 >B8G291.1 B8G291_DESHD Nitrogenase iron protein EC=1.18.6.1 Length = 292 Score = 382 bits (980), Expect = e-104, Method: Compositional matrix adjust. Identities = 180/249 (72%), Positives = 206/249 (82%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDNHAVRAQDVIFEGFNGIYXXXXXXXXXXX 60 VMQFGCDPKSDSTNTLRGG+YIPTVLDTLR+ V+A +VIFEGFNGIY Sbjct: 36 VMQFGCDPKSDSTNTLRGGKYIPTVLDTLREKSVVKASEVIFEGFNGIYCVEAGGPAPGV 95 Query: 61 XXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVTSAD 120 IIT+V L KQQ++F LDLDFVIYDVLGDVVCGGFA+P+REGIAEHV+TV+S+D Sbjct: 96 GCAGRGIITAVELFKQQKVFASLDLDFVIYDVLGDVVCGGFAIPIREGIAEHVFTVSSSD 155 Query: 121 FMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYVPRS 180 FM+IYA+NNLFKGI+KYS GGALLGGVIANSIN YAK IVDDFV+RT TQVVEY+PRS Sbjct: 156 FMSIYASNNLFKGIKKYSNSGGALLGGVIANSINQGYAKAIVDDFVERTQTQVVEYIPRS 215 Query: 181 VTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLDTQELRQWAAEWGKQ 240 +TVTQSELQGKTTIEAAP+SEQAKVY LA KI +H SKVPAPL+ +EL++WA W Q Sbjct: 216 ITVTQSELQGKTTIEAAPESEQAKVYTRLAEKIVEHKSSKVPAPLEIKELQEWATSWSDQ 275 Query: 241 LVALESGLV 249 L+ALE G+V Sbjct: 276 LLALERGVV 284 >B9M031.1 B9M031_GEOSF Nitrogenase iron protein EC=1.18.6.1 Length = 292 Score = 372 bits (955), Expect = e-101, Method: Compositional matrix adjust. Identities = 177/253 (69%), Positives = 207/253 (81%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDNHAVRAQDVIFEGFNGIYXXXXXXXXXXX 60 VMQ GCDPKSDST TLRGG YIPT+LDTLRD +V++ ++IF+GFNGIY Sbjct: 37 VMQIGCDPKSDSTTTLRGGGYIPTILDTLRDKKSVKSNEIIFDGFNGIYCVEAGGPAPGV 96 Query: 61 XXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVTSAD 120 IITSV LLKQ R+F+ELDLD V+YDVLGDVVCGGFAVP+REGIA+HV+TV+S+D Sbjct: 97 GCAGRGIITSVELLKQLRVFDELDLDVVVYDVLGDVVCGGFAVPIREGIADHVFTVSSSD 156 Query: 121 FMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYVPRS 180 FMAIYAANNLFKGIQKYS +GGALLGGVIANS+N YAK+I+DDFV +T TQV+EYVPRS Sbjct: 157 FMAIYAANNLFKGIQKYSNNGGALLGGVIANSVNTGYAKEIIDDFVTQTKTQVIEYVPRS 216 Query: 181 VTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLDTQELRQWAAEWGKQ 240 VTVTQSELQGKTTIEA P+SEQAKVYR LA+KI DHT SKVP PL+ +EL +WA +W Sbjct: 217 VTVTQSELQGKTTIEAFPESEQAKVYRGLAQKIYDHTESKVPTPLNDKELHEWAFKWADT 276 Query: 241 LVALESGLVAPAA 253 L+A+E+G V A Sbjct: 277 LLAMETGEVRSKA 289 >Q2RW96.1 Q2RW96_RHORT Nitrogenase iron protein EC=1.18.6.1 Length = 291 Score = 369 bits (947), Expect = e-100, Method: Compositional matrix adjust. Identities = 174/245 (71%), Positives = 201/245 (82%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDNHAVRAQDVIFEGFNGIYXXXXXXXXXXX 60 VMQFGCDPKSDSTNTLRGG+YIP+VLD LR+N V A + IF+GF GIY Sbjct: 36 VMQFGCDPKSDSTNTLRGGDYIPSVLDLLRENARVDAHEAIFQGFGGIYCVEAGGPAPGV 95 Query: 61 XXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVTSAD 120 IIT+V LLKQQ +FEELDLD+VI+DVLGDVVCGGFAVP+REGIAEHV+TV+S+D Sbjct: 96 GCAGRGIITAVELLKQQNVFEELDLDYVIFDVLGDVVCGGFAVPIREGIAEHVFTVSSSD 155 Query: 121 FMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYVPRS 180 FMAIYAANNLFKGIQKYS GGALLGGVIANSIN + +DI+DDFV RT TQVV+YVPRS Sbjct: 156 FMAIYAANNLFKGIQKYSNAGGALLGGVIANSINTDFHRDIIDDFVARTQTQVVQYVPRS 215 Query: 181 VTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLDTQELRQWAAEWGKQ 240 +TVTQ+ELQG+TTIEAAP+S QA++YR LAR IADHT SKVP PL+ QELR W+A W Q Sbjct: 216 LTVTQAELQGRTTIEAAPESAQAEIYRTLARSIADHTDSKVPTPLNAQELRDWSASWANQ 275 Query: 241 LVALE 245 L+ +E Sbjct: 276 LIEIE 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,357,291,822 Number of extensions: 49355572 Number of successful extensions: 151766 Number of sequences better than 1.0e-05: 10366 Number of HSP's gapped: 143414 Number of HSP's successfully gapped: 10366 Length of query: 257 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 124 Effective length of database: 1,807,701,208 Effective search space: 224154949792 Effective search space used: 224154949792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)