BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0024.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9X2B9.1 B9X2B9_9BACL NifB protein 264 6e-69 Q9AKT9.1 Q9AKT9_PAEAZ NifB2 protein 260 9e-68 B9X299.1 B9X299_9BACL NifB protein 259 2e-67 B2J6Z1.1 B2J6Z1_NOSP7 Nitrogenase cofactor biosynthesis protein ... 258 5e-67 Q56JU5.1 Q56JU5_9BACL NifB 257 1e-66 >B9X2B9.1 B9X2B9_9BACL NifB protein Length = 458 Score = 264 bits (675), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 135/251 (53%), Positives = 172/251 (68%) Query: 9 AMQDIHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPGE 68 A ++I RHPCY E AH FARMH+ VAP V+ESRPGVVS VL+P + Sbjct: 12 AEEEISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRPGVVSEVLTPQQ 71 Query: 69 AYRQVQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLPD 128 A R+V+ L QL+VVGIAGPGDPLANP TF+TF + +PD+ LCLSTNGL L Sbjct: 72 AERKVKGVAAQLMQLSVVGIAGPGDPLANPEATFDTFARVKEHVPDVSLCLSTNGLTLYR 131 Query: 129 YLEEIKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAVA 188 Y++EI E GIRHVT+T+NA+DP +G IY G Y G++AAE+LI+RQL G+ +A Sbjct: 132 YVDEIVELGIRHVTITINAIDPDVGREIYPWAVDEGIRYEGREAAELLITRQLLGVEMLA 191 Query: 189 ALGIKVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISPGSRFERDGRQPPDPE 248 GI VKVNS++IPG+ND HL+ VS+ VK GA HN+ PLII+PGS++E+DGR+ P P+ Sbjct: 192 KRGILVKVNSIMIPGVNDHHLVAVSKKVKELGATLHNVTPLIIAPGSQYEKDGRKAPRPK 251 Query: 249 LTLKVQEDSSR 259 VQE R Sbjct: 252 ELHTVQEQLGR 262 >Q9AKT9.1 Q9AKT9_PAEAZ NifB2 protein Length = 458 Score = 260 bits (665), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 133/251 (52%), Positives = 172/251 (68%) Query: 8 SAMQDIHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPG 67 +A DI RHPCY E AH FARMH+ VAP V+ESRPGVVS VL+P Sbjct: 12 AAEDDISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRPGVVSEVLTPE 71 Query: 68 EAYRQVQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLP 127 +A R+V+ L QL+VVGIAGPGDPLAN +TF+TF + + +PD+ CLSTNGL L Sbjct: 72 QAERKVKGVAAQLMQLSVVGIAGPGDPLANADKTFDTFARVKKHVPDVITCLSTNGLTLY 131 Query: 128 DYLEEIKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAV 187 +++ I E GI HVT+T+NA+DP +G IY + G Y G++AA +LISRQL+G+ + Sbjct: 132 RHIDRIVELGIGHVTITINAIDPDVGKEIYPWVSDEGVRYEGREAAALLISRQLQGVEEL 191 Query: 188 AALGIKVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISPGSRFERDGRQPPDP 247 A GI VKVNS+LIPGIND HL+EVS+ VK GA HN+ PLII+PGS++E+DGR+ P P Sbjct: 192 AKRGILVKVNSILIPGINDKHLVEVSKKVKELGATLHNVTPLIIAPGSQYEKDGRKAPRP 251 Query: 248 ELTLKVQEDSS 258 ++QE S Sbjct: 252 RELNQLQEQLS 262 >B9X299.1 B9X299_9BACL NifB protein Length = 458 Score = 259 bits (663), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 130/251 (51%), Positives = 173/251 (68%) Query: 8 SAMQDIHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPG 67 +A DI RHPCY E AH FARMH+ VAP V+ESRPGVVS VL+P Sbjct: 12 AAEDDISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRPGVVSEVLTPE 71 Query: 68 EAYRQVQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLP 127 +A R+V+ L QL+VVGIAGPGDPLAN +TF+TF + + +PD+ CLSTNGL L Sbjct: 72 QAERKVKGVAAQLMQLSVVGIAGPGDPLANADKTFDTFARVKKHVPDVMTCLSTNGLTLY 131 Query: 128 DYLEEIKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAV 187 +++ I E GI HVT+T+NA+DP +G IY ++ G Y G++AA +LISRQL+ + + Sbjct: 132 RHIDRIVELGIGHVTITINAIDPDVGKEIYPWVFDEGVRYEGREAAALLISRQLQEVEEL 191 Query: 188 AALGIKVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISPGSRFERDGRQPPDP 247 A GI VKVNS++IPG+ND HL+EVS+ VK GA HN+ PLII+PGS++E+DGR+ P P Sbjct: 192 AKRGILVKVNSIMIPGVNDKHLVEVSKKVKELGATLHNVTPLIIAPGSQYEKDGRKAPRP 251 Query: 248 ELTLKVQEDSS 258 + ++QE S Sbjct: 252 KELNQLQEQLS 262 >B2J6Z1.1 B2J6Z1_NOSP7 Nitrogenase cofactor biosynthesis protein NifB Length = 483 Score = 258 bits (658), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 131/248 (52%), Positives = 171/248 (68%), Gaps = 2/248 (0%) Query: 13 IHRHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPGEAYRQ 72 I +HPCY E AH +ARMHVAVAP +ESRPGVVS +L+P EA + Sbjct: 45 IAKHPCYSEDAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEAAHK 104 Query: 73 VQDTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLPDYLEE 132 +PQ+TVVGIAGPGDPLANP +TF TF L++ + PD++LCLSTNGL LPDY++ Sbjct: 105 ALVIGGKIPQMTVVGIAGPGDPLANPEKTFRTFELIAAQAPDIKLCLSTNGLMLPDYIDR 164 Query: 133 IKECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAVAALGI 192 IK+ I HVT+T+N VDP IG IY ++YR K YRG + A+IL RQ+EG++A+ I Sbjct: 165 IKQLNIDHVTITINMVDPEIGAKIYPWVHYRRKRYRGLEGAKILHERQMEGLQALKDADI 224 Query: 193 KVKVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISP--GSRFERDGRQPPDPELT 250 KVNSV+IPGIND HL+EV+RV++ GAF HNIMPLI +P G+ F G++ P P+ Sbjct: 225 LCKVNSVMIPGINDHHLVEVNRVIREKGAFLHNIMPLISAPEHGTHFGLIGQRGPTPKEL 284 Query: 251 LKVQEDSS 258 +VQ++ S Sbjct: 285 KEVQDNCS 292 >Q56JU5.1 Q56JU5_9BACL NifB Length = 473 Score = 257 bits (656), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 133/244 (54%), Positives = 166/244 (68%) Query: 15 RHPCYDELAHEVFARMHVAVAPXXXXXXXXXXXXXXXVSESRPGVVSRVLSPGEAYRQVQ 74 RHPCY+E AH FARM + VAP V+ESRPGVVS +L+P A R+ Sbjct: 29 RHPCYNEDAHRYFARMRLPVAPACNIQCHYCNRKFDCVNESRPGVVSELLTPELAARKTI 88 Query: 75 DTLKVLPQLTVVGIAGPGDPLANPVQTFETFRLLSREMPDLQLCLSTNGLKLPDYLEEIK 134 L QL+VVGIAGPGDPLANP TFETFR + + D+ CLSTNGL L ++EEI Sbjct: 89 GVAAQLMQLSVVGIAGPGDPLANPKATFETFRQVRDSVKDVIFCLSTNGLTLIRHVEEIV 148 Query: 135 ECGIRHVTVTMNAVDPVIGGLIYHSIYYRGKAYRGKDAAEILISRQLEGIRAVAALGIKV 194 GI HVT+T+NAVDP IG IY +Y G Y G++ A +LI RQLEG+ +++ G+ V Sbjct: 149 NLGISHVTITINAVDPCIGSRIYGWVYDGGVRYEGEEGAALLIQRQLEGLSMLSSRGVLV 208 Query: 195 KVNSVLIPGINDGHLLEVSRVVKAAGAFSHNIMPLIISPGSRFERDGRQPPDPELTLKVQ 254 KVNSVLIP +ND HL EV+RVVK GA HNIMPLII+PGSR+E++GRQ P P++ +VQ Sbjct: 209 KVNSVLIPDVNDAHLPEVARVVKGHGAAIHNIMPLIIAPGSRYEQEGRQAPRPQVVRQVQ 268 Query: 255 EDSS 258 E + Sbjct: 269 EQCA 272 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,567,461,989 Number of extensions: 59706865 Number of successful extensions: 129035 Number of sequences better than 1.0e-05: 263 Number of HSP's gapped: 129462 Number of HSP's successfully gapped: 263 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)