BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0023.p (162 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q7WTA9.1 Q7WTA9_PAEAZ NifB1 protein (Fragment) 122 7e-27 B9X299.1 B9X299_9BACL NifB protein 122 7e-27 B9X2B9.1 B9X2B9_9BACL NifB protein 122 8e-27 B9X241.1 B9X241_9BACL NifB protein (Fragment) 122 8e-27 Q9AKT9.1 Q9AKT9_PAEAZ NifB2 protein 122 1e-26 >Q7WTA9.1 Q7WTA9_PAEAZ NifB1 protein (Fragment) Length = 320 Score = 122 bits (307), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 67/158 (42%), Positives = 99/158 (62%), Gaps = 10/158 (6%) Query: 3 ALPVYTLEERENELQKLDNELQVRRDRRQN--GTAPGSGGIRIAVASRGGGKVNQHFGHA 60 A PV L+ RE Q+LD +++ R ++N + R+AVASRGG KVNQHFGHA Sbjct: 160 ADPVIDLDAREQFQQELDIKIRQRLIAKENRRTILDENKKTRVAVASRGGDKVNQHFGHA 219 Query: 61 REFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGIG 120 EF++++ + +G+R+IQAYC+GTA C G++ L+E +L DCR+LL +GIG Sbjct: 220 TEFMIFDTDGTEVKFIGIRKIQAYCHGTADCNGDKDET--LQEITSILSDCRILLSSGIG 277 Query: 121 PVPQEVLHRAEITVIVTKDAIESSL---VKYGRLLSYF 155 P+ VL++A + +V K I+ ++ VKYG SYF Sbjct: 278 DAPRAVLNKAGVLPLVRKGGIQEAILESVKYG---SYF 312 >B9X299.1 B9X299_9BACL NifB protein Length = 458 Score = 122 bits (307), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 63/155 (40%), Positives = 97/155 (62%), Gaps = 4/155 (2%) Query: 3 ALPVYTLEERENELQKLDNELQVRRDRRQN--GTAPGSGGIRIAVASRGGGKVNQHFGHA 60 A PV L+ RE ++LD +++ R ++N R+AVASRGG KVNQHFGHA Sbjct: 298 ADPVIDLDAREQFQKELDGKIRQRLIAKENRRTILDEDKKTRVAVASRGGDKVNQHFGHA 357 Query: 61 REFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGIG 120 EF++++ + +G+R+IQAYC+GTA C G++ L+E +L DCR+LL +GIG Sbjct: 358 TEFMIFDTDGTEVKFIGIRKIQAYCHGTADCNGDKDET--LQEITSILSDCRILLSSGIG 415 Query: 121 PVPQEVLHRAEITVIVTKDAIESSLVKYGRLLSYF 155 P+ VL+RA + +V K I+ ++++ + SYF Sbjct: 416 DAPRAVLNRAGVLPLVRKGGIQEAILESVKYGSYF 450 >B9X2B9.1 B9X2B9_9BACL NifB protein Length = 458 Score = 122 bits (307), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 67/165 (40%), Positives = 98/165 (59%), Gaps = 17/165 (10%) Query: 4 LPVYTLEERENELQKLDNELQVRRD-------------RRQNGTAPGSGGIRIAVASRGG 50 P+ +EE N L +D +Q + D R+ G G+ RIAVA+RGG Sbjct: 290 FPLEAMEE--NPLINMDARMQFQSDLDIKIRERNAAKQARREGVRQGAVASRIAVATRGG 347 Query: 51 GKVNQHFGHAREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRD 110 KVNQHFGHA EF++Y+ +L+GVR+IQAYC+GTA C G++ A L+E + +L D Sbjct: 348 DKVNQHFGHATEFMIYDTDGTDIKLIGVRKIQAYCHGTADCNGDK--AATLQEILSILSD 405 Query: 111 CRLLLCAGIGPVPQEVLHRAEITVIVTKDAIESSLVKYGRLLSYF 155 CR+LLC+GIG P+ L++ I +V K I ++++ + SYF Sbjct: 406 CRILLCSGIGEGPKIHLNKRGILPLVRKGDIREAILESVKYSSYF 450 >B9X241.1 B9X241_9BACL NifB protein (Fragment) Length = 304 Score = 122 bits (307), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 63/138 (45%), Positives = 90/138 (65%), Gaps = 5/138 (3%) Query: 18 KLDNELQVRRDRRQNGTAPGSGGIRIAVASRGGGKVNQHFGHAREFLVYEVKAESARLLG 77 K+ +Q +R R AP IR+AVA+RGG KVNQHFGHA EFLVY+ +LLG Sbjct: 164 KIRERVQAKRARLLQENAPK---IRVAVATRGGDKVNQHFGHATEFLVYDTDGAVVQLLG 220 Query: 78 VRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGIGPVPQEVLHRAEITVIVT 137 VR+IQAYC+G A C G++ A L+E + +L DCR+LLC+GIG P+ L++ + +V Sbjct: 221 VRKIQAYCHGKADCNGDK--AATLQEIISILSDCRILLCSGIGEGPRTSLNKIGVLPLVR 278 Query: 138 KDAIESSLVKYGRLLSYF 155 K I+ ++++ + SYF Sbjct: 279 KGGIQEAILESVKYSSYF 296 >Q9AKT9.1 Q9AKT9_PAEAZ NifB2 protein Length = 458 Score = 122 bits (306), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 66/158 (41%), Positives = 99/158 (62%), Gaps = 10/158 (6%) Query: 3 ALPVYTLEERENELQKLDNELQVRRDRRQN--GTAPGSGGIRIAVASRGGGKVNQHFGHA 60 A PV L+ RE Q+LD++++ R ++N + R+AVASRGG KVNQHFGHA Sbjct: 298 ADPVIDLDAREQFQQELDSKIRQRLIAKENRRSILDENKKTRVAVASRGGDKVNQHFGHA 357 Query: 61 REFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGIG 120 EF++++ + +G+R+IQAYC+GTA C G++ L+E +L DCR+LL +GIG Sbjct: 358 TEFMIFDTDGAEVKFIGIRKIQAYCHGTADCNGDK--GETLQEITSILSDCRILLSSGIG 415 Query: 121 PVPQEVLHRAEITVIVTKDAIESSL---VKYGRLLSYF 155 P+ L++A + +V K I+ ++ VKYG SYF Sbjct: 416 DAPRAALNKAGVLPLVRKGGIQEAILESVKYG---SYF 450 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 949,925,920 Number of extensions: 36764784 Number of successful extensions: 101466 Number of sequences better than 1.0e-05: 143 Number of HSP's gapped: 101423 Number of HSP's successfully gapped: 143 Length of query: 162 Length of database: 3,060,696,203 Length adjustment: 122 Effective length of query: 40 Effective length of database: 1,911,332,373 Effective search space: 76453294920 Effective search space used: 76453294920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)