BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0022.p (342 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9E389.1 B9E389_CLOK1 Putative uncharacterized protein 414 e-114 A5MZ94.1 A5MZ94_CLOK5 O-acetyl-L-homoserine sulfhydrylase-relate... 414 e-114 B9M072.1 B9M072_GEOSF O-acetylhomoserine aminocarboxypropyltrans... 407 e-112 A6LR29.1 A6LR29_CLOB8 Cysteine synthase EC=2.5.1.47 402 e-110 A3DFR6.1 A3DFR6_CLOTH O-acetylhomoserine aminocarboxypropyltrans... 394 e-107 >B9E389.1 B9E389_CLOK1 Putative uncharacterized protein Length = 442 Score = 414 bits (1063), Expect = e-114, Method: Compositional matrix adjust. Identities = 192/333 (57%), Positives = 243/333 (72%), Gaps = 1/333 (0%) Query: 3 MTDKKLGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRI 62 M+ + L FDT+K+R GY+S+EHN++V+VPIY+TA++DLGS ER KLF E G++Y+R+ Sbjct: 1 MSKENLRFDTIKIRGGYNSKEHNHSVSVPIYETAAFDLGSTERAGKLFSFSELGFIYSRL 60 Query: 63 GNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALK 122 NPTV VLE+R+ ALD TY LLN+AE GGRILTTP LYGGT D K Sbjct: 61 SNPTVEVLEKRVAALDGAAGDIALSSGMAAITYTLLNVAEDGGRILTTPYLYGGTLDGFK 120 Query: 123 HLYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGI 182 LYPKFG+ +D ED ++PEA + I PDTKAI VESISNPN+ V DIE IA+ AH+ I Sbjct: 121 KLYPKFGIKIDKFEDFNNPEALAKQIKPDTKAIFVESISNPNAAVADIEKIAKVAHEYDI 180 Query: 183 PLVIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFE 242 PL++DNT TPYL + +GADIVIYSATKA+ GHG + G+ILE+G FNW NG+FP F Sbjct: 181 PLIVDNTLATPYLLNPIKYGADIVIYSATKALNGHGNVIAGIILESGKFNWENGKFPQFL 240 Query: 243 EPQYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSER 302 EP Y LR+ E GR R+ +EVFPD PF R+RLNYL YFGA L PFDA+L+L G+ETLSER Sbjct: 241 EPYYTLRDPE-GRARNFIEVFPDFPFITRIRLNYLNYFGAALSPFDAYLVLLGLETLSER 299 Query: 303 ISKQVANALRIVDYLQKHDQVSWVSHPVAEGNP 335 I KQ++N +I+ YLQ+ D V W+ +P +P Sbjct: 300 IEKQLSNTKKIISYLQESDNVQWIKYPSLNDSP 332 >A5MZ94.1 A5MZ94_CLOK5 O-acetyl-L-homoserine sulfhydrylase-related protein Length = 442 Score = 414 bits (1063), Expect = e-114, Method: Compositional matrix adjust. Identities = 192/333 (57%), Positives = 243/333 (72%), Gaps = 1/333 (0%) Query: 3 MTDKKLGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRI 62 M+ + L FDT+K+R GY+S+EHN++V+VPIY+TA++DLGS ER KLF E G++Y+R+ Sbjct: 1 MSKENLRFDTIKIRGGYNSKEHNHSVSVPIYETAAFDLGSTERAGKLFSFSELGFIYSRL 60 Query: 63 GNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALK 122 NPTV VLE+R+ ALD TY LLN+AE GGRILTTP LYGGT D K Sbjct: 61 SNPTVEVLEKRVAALDGAAGDIALSSGMAAITYTLLNVAEDGGRILTTPYLYGGTLDGFK 120 Query: 123 HLYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGI 182 LYPKFG+ +D ED ++PEA + I PDTKAI VESISNPN+ V DIE IA+ AH+ I Sbjct: 121 KLYPKFGIKIDKFEDFNNPEALAKQIKPDTKAIFVESISNPNAAVADIEKIAKVAHEYDI 180 Query: 183 PLVIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFE 242 PL++DNT TPYL + +GADIVIYSATKA+ GHG + G+ILE+G FNW NG+FP F Sbjct: 181 PLIVDNTLATPYLLNPIKYGADIVIYSATKALNGHGNVIAGIILESGKFNWENGKFPQFL 240 Query: 243 EPQYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSER 302 EP Y LR+ E GR R+ +EVFPD PF R+RLNYL YFGA L PFDA+L+L G+ETLSER Sbjct: 241 EPYYTLRDPE-GRARNFIEVFPDFPFITRIRLNYLNYFGAALSPFDAYLVLLGLETLSER 299 Query: 303 ISKQVANALRIVDYLQKHDQVSWVSHPVAEGNP 335 I KQ++N +I+ YLQ+ D V W+ +P +P Sbjct: 300 IEKQLSNTKKIISYLQESDNVQWIKYPSLNDSP 332 >B9M072.1 B9M072_GEOSF O-acetylhomoserine aminocarboxypropyltransferase EC=2.5.1.49 Length = 437 Score = 407 bits (1046), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/333 (59%), Positives = 252/333 (75%), Gaps = 1/333 (0%) Query: 3 MTDKKLGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRI 62 M L FDT+KVR GYD + HN AV+VPIYQTAS++LG ER E+L +E G++YTR+ Sbjct: 1 MAYGDLSFDTVKVRGGYDPKAHNLAVSVPIYQTASFELGDTERVERLLRFKELGFVYTRL 60 Query: 63 GNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALK 122 GNPTV VLE+R+ ALD TYAL N+AEGGGRILTT LYGGT D+ K Sbjct: 61 GNPTVQVLEERIAALDGGSAAIAVGSGMAAVTYALFNVAEGGGRILTTRNLYGGTEDSFK 120 Query: 123 HLYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGI 182 L+P FG+ +D V+D+DD + FRR I P+T+AI VESISNPN+T+LD+E IA+ AH+NGI Sbjct: 121 KLFPTFGIGIDQVDDADDVDQFRRGIKPETRAIFVESISNPNATLLDLERIADVAHENGI 180 Query: 183 PLVIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFE 242 PL++DNTF TPYLF+ HGADIV+YSATKA+GGHG T+ GVI+E G F+W NG+FP F Sbjct: 181 PLIVDNTFATPYLFNPLKHGADIVVYSATKALGGHGNTIAGVIVEGGTFDWGNGKFPQFT 240 Query: 243 EPQYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSER 302 +P YLLR+ +TG ERS LE+ + PFT RVRLNYL YFGAVL PFDA+L+LQG+ETLSER Sbjct: 241 KPHYLLRD-DTGVERSFLEITREFPFTLRVRLNYLVYFGAVLSPFDAYLILQGVETLSER 299 Query: 303 ISKQVANALRIVDYLQKHDQVSWVSHPVAEGNP 335 + KQ+ N +I+ YL+ +V+W+ HP A+ +P Sbjct: 300 VQKQMTNTEKIIRYLESKGEVAWIKHPSAKESP 332 >A6LR29.1 A6LR29_CLOB8 Cysteine synthase EC=2.5.1.47 Length = 432 Score = 402 bits (1032), Expect = e-110, Method: Compositional matrix adjust. Identities = 183/326 (56%), Positives = 247/326 (75%), Gaps = 1/326 (0%) Query: 10 FDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRIGNPTVAV 69 FDT+KVR GY+ +EHN AV+VPIY TAS+++G ER ++L + E G++Y+R+GNPTV+V Sbjct: 6 FDTIKVRGGYNPKEHNDAVSVPIYATASFEVGEAERFDRLASLSEEGFIYSRLGNPTVSV 65 Query: 70 LEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALKHLYPKFG 129 LE R+ +LD TYALL +AEGGGRILTT +LYGGT DALK LYPKFG Sbjct: 66 LENRIASLDGGIAAVGVASGMAAITYALLAVAEGGGRILTTHQLYGGTVDALKKLYPKFG 125 Query: 130 VHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGIPLVIDNT 189 + +D +ED +D E FR+ I DTKAI +E+ISNPN+ + DI AIA+ AH+N IPL++DNT Sbjct: 126 IQIDRIEDDNDIEEFRKSIKEDTKAIFIETISNPNAALYDIGAIAKIAHENNIPLIVDNT 185 Query: 190 FGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFEEPQYLLR 249 F TPYLF+ +GADIVIYSATKA+ GHG + GVI+++G FNWANGR+P F EP + L+ Sbjct: 186 FATPYLFNPIKYGADIVIYSATKALSGHGNVIAGVIVDSGKFNWANGRYPQFSEPYFTLK 245 Query: 250 EAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSERISKQVAN 309 + E G ERS ++VF ++ F ++RL+YL YFGAVL PFDA+L+L G+ETLSER+ KQ+ N Sbjct: 246 DLE-GNERSYIDVFKESAFAGKIRLDYLTYFGAVLSPFDAYLILIGLETLSERVQKQINN 304 Query: 310 ALRIVDYLQKHDQVSWVSHPVAEGNP 335 +I+DYL+ + V+W+++P EG+P Sbjct: 305 TRKIIDYLKNEESVAWINYPEIEGSP 330 >A3DFR6.1 A3DFR6_CLOTH O-acetylhomoserine aminocarboxypropyltransferase EC=2.5.1.49 Length = 438 Score = 394 bits (1011), Expect = e-107, Method: Compositional matrix adjust. Identities = 185/326 (56%), Positives = 237/326 (72%), Gaps = 1/326 (0%) Query: 10 FDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRIGNPTVAV 69 FDT ++RAGYD +EHNYAV+ PIYQT S+D VE + LFG+ E G LYTRIGNPTVAV Sbjct: 12 FDTQRLRAGYDPKEHNYAVSPPIYQTTSFDFRDVEHAKALFGLRELGNLYTRIGNPTVAV 71 Query: 70 LEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALKHLYPKFG 129 LEQR+ ALD +Y LLN+AEGGGRILT+P LYGG+ D+ K +YPKFG Sbjct: 72 LEQRVAALDGASGAVALASGMAAISYTLLNVAEGGGRILTSPYLYGGSADSFKKVYPKFG 131 Query: 130 VHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGIPLVIDNT 189 + DF ++ ++PE +I PDTKAI VESISNPN+ +LDI+AIA+ AH++GIPL++DNT Sbjct: 132 ITFDFAKNIENPEKLSEEIKPDTKAIYVESISNPNAALLDIDAIAKVAHEHGIPLIVDNT 191 Query: 190 FGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFEEPQYLLR 249 TPYL++ +HGADIV+YSATK + GHG + G++LE+G FNW + +FP F E Y LR Sbjct: 192 VATPYLYNPISHGADIVVYSATKGLTGHGNVIAGLVLESGKFNWQSDKFPQFSEKYYTLR 251 Query: 250 EAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSERISKQVAN 309 + RS LEVFP+APFT R+R NYL YFGA L PFDA+L+L G+ETLSER+ KQV N Sbjct: 252 DINDNY-RSFLEVFPEAPFTGRIRFNYLNYFGAALSPFDAYLVLIGLETLSERVEKQVRN 310 Query: 310 ALRIVDYLQKHDQVSWVSHPVAEGNP 335 A + +YL+ H V WV +P + +P Sbjct: 311 ASILAEYLKSHKSVEWVRYPGLKDSP 336 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,107,482,000 Number of extensions: 89497027 Number of successful extensions: 213781 Number of sequences better than 1.0e-05: 4294 Number of HSP's gapped: 205295 Number of HSP's successfully gapped: 4326 Length of query: 342 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 206 Effective length of database: 1,779,438,163 Effective search space: 366564261578 Effective search space used: 366564261578 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)