BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0018.p (493 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LR27.1 A6LR27_CLOB8 Nitrogenase EC=1.18.6.1 748 0.0 B9DZW7.1 B9DZW7_CLOK1 Putative uncharacterized protein 647 0.0 A5N6E7.1 A5N6E7_CLOK5 NifD1 EC=1.18.6.1 647 0.0 A3DFR2.1 A3DFR2_CLOTH Nitrogenase EC=1.18.6.1 576 e-162 B4BA92.1 B4BA92_CLOTM Nitrogenase EC=1.18.6.1 576 e-162 >A6LR27.1 A6LR27_CLOB8 Nitrogenase EC=1.18.6.1 Length = 493 Score = 748 bits (1931), Expect = 0.0, Method: Compositional matrix adjust. Identities = 345/493 (69%), Positives = 406/493 (82%) Query: 1 MSKVNLNTPEVPVRELRLGSITGFAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFC 60 M +NL V +RE RLGSITGF G+A L++C++ G +KD R FSQCMGC++G+AFC Sbjct: 1 MGTINLKESSVTIRETRLGSITGFNGSAKELVTCAHAGKLKDSPRKFSQCMGCNAGHAFC 60 Query: 61 QLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTNIEEMDTVFGGA 120 QLSMI DA +VNHAP+GCAGDFFG+NF YRV QM+R LP IGRYF+T IEE DTVFG A Sbjct: 61 QLSMIRDAVVVNHAPIGCAGDFFGFNFTYRVEQMKRELPATIGRYFSTCIEEKDTVFGAA 120 Query: 121 RKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKSK 180 +KLE T++ AY+R KPNAIFVTTSCASGIIG+DV+++T ++KELGIPV++C CEGF+SK Sbjct: 121 KKLEDTVREAYKRTKPNAIFVTTSCASGIIGEDVDAITENLSKELGIPVISCSCEGFRSK 180 Query: 181 IWTSGFDSAYHSIVRKIVKPPEKKTNKVNIINFWGSDVFSGLLNKLGYEADYIVPFSTVA 240 IWT+GFD+AYHSI+RK+VKPP KKTNK+N+ NFWGS +F LN+LGYEA YIVPFSTVA Sbjct: 181 IWTTGFDAAYHSILRKLVKPPRKKTNKINVANFWGSHIFDEFLNRLGYEAQYIVPFSTVA 240 Query: 241 QLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLDRX 300 +LE ISEAAATI ICP+L TYLGAALEQ YGV EIKAP AYG+ GTD WMRE+GRVL + Sbjct: 241 ELEYISEAAATIHICPSLSTYLGAALEQEYGVKEIKAPPAYGIEGTDIWMREIGRVLGKE 300 Query: 301 XXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAIFH 360 VLP+LE YR KL G Y+TAG+AHGHA+IA+LRELG+ VQGAAIFH Sbjct: 301 EEIEEIIKEEHERVLPKLEEYREKLKGKIAYVTAGAAHGHAIIAMLRELGLIVQGAAIFH 360 Query: 361 HDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDLMIARHGGMTHW 420 HDP+YDN D +D L TV YGD+ +YNVCNKQAYEL NILNRV PDLMIARHGGMT W Sbjct: 361 HDPLYDNKDKTSDALAQTVNIYGDIKHYNVCNKQAYELVNILNRVKPDLMIARHGGMTMW 420 Query: 421 GAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTMPYTKWWMEQNP 480 GAKLGIPTLLIGDE F +GY+G+LNYA RI+ETLDNREFV+NLAKHSTMPYTKWW++Q+P Sbjct: 421 GAKLGIPTLLIGDEQFGFGYQGVLNYASRIVETLDNREFVSNLAKHSTMPYTKWWLDQDP 480 Query: 481 YTFLGGNVHVEAY 493 +TFLGGN VE Y Sbjct: 481 FTFLGGNNSVENY 493 >B9DZW7.1 B9DZW7_CLOK1 Putative uncharacterized protein Length = 488 Score = 647 bits (1668), Expect = 0.0, Method: Compositional matrix adjust. Identities = 300/486 (61%), Positives = 370/486 (76%), Gaps = 1/486 (0%) Query: 4 VNLNTPEVPVRELRLGSITGFAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFCQLS 63 +N+ +PEV +RE RLGSITGFAGTA L + S + D RSFSQC+GC+SG + CQL Sbjct: 3 INIKSPEVEIRESRLGSITGFAGTARELCNRSKGKRLCDRRRSFSQCLGCNSGYSLCQLI 62 Query: 64 MITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTNIEEMDTVFGGARKL 123 MI D+ ++NHAP+GC D +NF RVGQ++RNLP R NTN+ E DT++GG KL Sbjct: 63 MIQDSVVINHAPIGCCADLPDFNFTNRVGQIKRNLPLRNARLLNTNLTEKDTIYGGEEKL 122 Query: 124 EATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKSKIWT 183 TIK AY+R KPNAIFVTTSCASGIIGDDVE VTN+ KELGIP+V CEGF+SKIWT Sbjct: 123 RKTIKEAYKRFKPNAIFVTTSCASGIIGDDVEVVTNEAEKELGIPIVFVACEGFRSKIWT 182 Query: 184 SGFDSAYHSIVRKIVKPP-EKKTNKVNIINFWGSDVFSGLLNKLGYEADYIVPFSTVAQL 242 +GFD+AYH+I+RKIVKPP K+ + VN+INFWG+D+F+ L ++G + +YIVPFST+AQL Sbjct: 183 TGFDAAYHAILRKIVKPPVNKREDLVNVINFWGTDIFTELFGRIGLKPNYIVPFSTIAQL 242 Query: 243 ERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLDRXXX 302 E+ISEAAAT+QICPTLGTYL A LEQ YGVPE+KAP AYG+ GTD W RELGRV + Sbjct: 243 EKISEAAATVQICPTLGTYLAAGLEQEYGVPEVKAPPAYGLKGTDLWFRELGRVTGKEKE 302 Query: 303 XXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAIFHHD 362 + P+L+ R+KL G YITAG+AHGH+LIALL+EL + V GAAIFHHD Sbjct: 303 VEELIKSERERIAPKLKELRSKLKGKRVYITAGAAHGHSLIALLKELHMEVAGAAIFHHD 362 Query: 363 PVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDLMIARHGGMTHWGA 422 P DN DP ++ L H VK YGDV N+NVCNKQ +EL N+LNR+ PDL IARHGGM WG Sbjct: 363 PCCDNKDPRSESLPHVVKNYGDVKNFNVCNKQPFELVNVLNRLKPDLFIARHGGMAAWGP 422 Query: 423 KLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTMPYTKWWMEQNPYT 482 KLGIPT L+GDE F GY+GLLNY ++I+ L +EFVTN+A HS +PYT WW++Q+PYT Sbjct: 423 KLGIPTFLMGDEQFGLGYQGLLNYGDKIIGVLLTKEFVTNIAAHSKLPYTDWWLKQDPYT 482 Query: 483 FLGGNV 488 FLGG++ Sbjct: 483 FLGGDI 488 >A5N6E7.1 A5N6E7_CLOK5 NifD1 EC=1.18.6.1 Length = 488 Score = 647 bits (1668), Expect = 0.0, Method: Compositional matrix adjust. Identities = 300/486 (61%), Positives = 370/486 (76%), Gaps = 1/486 (0%) Query: 4 VNLNTPEVPVRELRLGSITGFAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFCQLS 63 +N+ +PEV +RE RLGSITGFAGTA L + S + D RSFSQC+GC+SG + CQL Sbjct: 3 INIKSPEVEIRESRLGSITGFAGTARELCNRSKGKRLCDRRRSFSQCLGCNSGYSLCQLI 62 Query: 64 MITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTNIEEMDTVFGGARKL 123 MI D+ ++NHAP+GC D +NF RVGQ++RNLP R NTN+ E DT++GG KL Sbjct: 63 MIQDSVVINHAPIGCCADLPDFNFTNRVGQIKRNLPLRNARLLNTNLTEKDTIYGGEEKL 122 Query: 124 EATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKSKIWT 183 TIK AY+R KPNAIFVTTSCASGIIGDDVE VTN+ KELGIP+V CEGF+SKIWT Sbjct: 123 RKTIKEAYKRFKPNAIFVTTSCASGIIGDDVEVVTNEAEKELGIPIVFVACEGFRSKIWT 182 Query: 184 SGFDSAYHSIVRKIVKPP-EKKTNKVNIINFWGSDVFSGLLNKLGYEADYIVPFSTVAQL 242 +GFD+AYH+I+RKIVKPP K+ + VN+INFWG+D+F+ L ++G + +YIVPFST+AQL Sbjct: 183 TGFDAAYHAILRKIVKPPVNKREDLVNVINFWGTDIFTELFGRIGLKPNYIVPFSTIAQL 242 Query: 243 ERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLDRXXX 302 E+ISEAAAT+QICPTLGTYL A LEQ YGVPE+KAP AYG+ GTD W RELGRV + Sbjct: 243 EKISEAAATVQICPTLGTYLAAGLEQEYGVPEVKAPPAYGLKGTDLWFRELGRVTGKEKE 302 Query: 303 XXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAIFHHD 362 + P+L+ R+KL G YITAG+AHGH+LIALL+EL + V GAAIFHHD Sbjct: 303 VEELIKSERERIAPKLKELRSKLKGKRVYITAGAAHGHSLIALLKELHMEVAGAAIFHHD 362 Query: 363 PVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDLMIARHGGMTHWGA 422 P DN DP ++ L H VK YGDV N+NVCNKQ +EL N+LNR+ PDL IARHGGM WG Sbjct: 363 PCCDNKDPRSESLPHVVKNYGDVKNFNVCNKQPFELVNVLNRLKPDLFIARHGGMAAWGP 422 Query: 423 KLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTMPYTKWWMEQNPYT 482 KLGIPT L+GDE F GY+GLLNY ++I+ L +EFVTN+A HS +PYT WW++Q+PYT Sbjct: 423 KLGIPTFLMGDEQFGLGYQGLLNYGDKIIGVLLTKEFVTNIAAHSKLPYTDWWLKQDPYT 482 Query: 483 FLGGNV 488 FLGG++ Sbjct: 483 FLGGDI 488 >A3DFR2.1 A3DFR2_CLOTH Nitrogenase EC=1.18.6.1 Length = 491 Score = 576 bits (1485), Expect = e-162, Method: Compositional matrix adjust. Identities = 277/488 (56%), Positives = 347/488 (71%), Gaps = 3/488 (0%) Query: 1 MSKVNLNTPEVPVRELRLGSITGFAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFC 60 MSK+NL+ PEV +RE+R+ SITG+ G A L+ + G+KD RSFSQC+GC++ A C Sbjct: 1 MSKINLSLPEVQIREIRINSITGYQGDAKELVE-AREFGLKDKERSFSQCLGCATSKAAC 59 Query: 61 QLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLP-PVIGRYFNTNIEEMDTVFGG 119 +I DAA+++H PVGCA + F YRV R + P R F+TN++E DTV+GG Sbjct: 60 MTVLIQDAAVISHGPVGCASCLHEFAFTYRVNYPLRGIERPTPRRIFSTNLKEKDTVYGG 119 Query: 120 ARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKS 179 KL TI+ YER NAIFV T+CA+GIIGDDVESV N+ +ELGIPVV FCEGF+S Sbjct: 120 NIKLANTIREVYERTHANAIFVLTTCAAGIIGDDVESVCNEAEEELGIPVVAIFCEGFRS 179 Query: 180 KIWTSGFDSAYHSIVRKIV-KPPEKKTNKVNIINFWGSDVFSGLLNKLGYEADYIVPFST 238 K+WT+GFD+AYH I RK++ KP ++ + +N+INFWGSDVF G + +YI+PFST Sbjct: 180 KVWTTGFDAAYHGIARKLIQKPRRRRDDMINVINFWGSDVFYEWFAPFGAKPNYIIPFST 239 Query: 239 VAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLD 298 V L+ SEAAAT+Q C TLG+YLGA LEQ +GVPEI A YG+A TD W R LG++L Sbjct: 240 VNGLKYASEAAATVQACSTLGSYLGAVLEQDFGVPEIPAAPPYGIAQTDRWFRALGKILG 299 Query: 299 RXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAI 358 + LP++EA R KLAG T Y+TAG+AHGHAL+ +L ELGI GAAI Sbjct: 300 KEEIAEKIIAEKKKEYLPKIEALREKLAGKTAYVTAGAAHGHALLDVLGELGIKAVGAAI 359 Query: 359 FHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDLMIARHGGMT 418 FHHDP+YD+G D L V YG+V NYNVCNKQ +EL N LNR+ PD+++ARHGGMT Sbjct: 360 FHHDPIYDSGREENDQLAQRVADYGNVFNYNVCNKQEFELVNALNRLRPDVLLARHGGMT 419 Query: 419 HWGAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTMPYTKWWMEQ 478 WGAKLGIP+LLIGDEH+S GY GL+NY ERILE ++N EFV NL KH+ PYTKWW+EQ Sbjct: 420 LWGAKLGIPSLLIGDEHYSMGYEGLVNYGERILEVIENDEFVKNLEKHAINPYTKWWLEQ 479 Query: 479 NPYTFLGG 486 PY FL G Sbjct: 480 PPYYFLKG 487 >B4BA92.1 B4BA92_CLOTM Nitrogenase EC=1.18.6.1 Length = 491 Score = 576 bits (1485), Expect = e-162, Method: Compositional matrix adjust. Identities = 277/488 (56%), Positives = 347/488 (71%), Gaps = 3/488 (0%) Query: 1 MSKVNLNTPEVPVRELRLGSITGFAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFC 60 MSK+NL+ PEV +RE+R+ SITG+ G A L+ + G+KD RSFSQC+GC++ A C Sbjct: 1 MSKINLSLPEVQIREIRINSITGYQGDAKELVE-AREFGLKDKERSFSQCLGCATSKAAC 59 Query: 61 QLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLP-PVIGRYFNTNIEEMDTVFGG 119 +I DAA+++H PVGCA + F YRV R + P R F+TN++E DTV+GG Sbjct: 60 MTVLIQDAAVISHGPVGCASCLHEFAFTYRVNYPLRGIERPTPRRIFSTNLKEKDTVYGG 119 Query: 120 ARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKS 179 KL TI+ YER NAIFV T+CA+GIIGDDVESV N+ +ELGIPVV FCEGF+S Sbjct: 120 NIKLANTIREVYERTHANAIFVLTTCAAGIIGDDVESVCNEAEEELGIPVVAIFCEGFRS 179 Query: 180 KIWTSGFDSAYHSIVRKIV-KPPEKKTNKVNIINFWGSDVFSGLLNKLGYEADYIVPFST 238 K+WT+GFD+AYH I RK++ KP ++ + +N+INFWGSDVF G + +YI+PFST Sbjct: 180 KVWTTGFDAAYHGIARKLIQKPRRRRDDMINVINFWGSDVFYEWFAPFGAKPNYIIPFST 239 Query: 239 VAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLD 298 V L+ SEAAAT+Q C TLG+YLGA LEQ +GVPEI A YG+A TD W R LG++L Sbjct: 240 VNGLKYASEAAATVQACSTLGSYLGAVLEQDFGVPEIPAAPPYGIAQTDRWFRALGKILG 299 Query: 299 RXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAI 358 + LP++EA R KLAG T Y+TAG+AHGHAL+ +L ELGI GAAI Sbjct: 300 KEEIAEKIIAEKKKEYLPKIEALREKLAGKTAYVTAGAAHGHALLDVLGELGIKAVGAAI 359 Query: 359 FHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYELANILNRVCPDLMIARHGGMT 418 FHHDP+YD+G D L V YG+V NYNVCNKQ +EL N LNR+ PD+++ARHGGMT Sbjct: 360 FHHDPIYDSGREENDQLAQRVADYGNVFNYNVCNKQEFELVNALNRLRPDVLLARHGGMT 419 Query: 419 HWGAKLGIPTLLIGDEHFSWGYRGLLNYAERILETLDNREFVTNLAKHSTMPYTKWWMEQ 478 WGAKLGIP+LLIGDEH+S GY GL+NY ERILE ++N EFV NL KH+ PYTKWW+EQ Sbjct: 420 LWGAKLGIPSLLIGDEHYSMGYEGLVNYGERILEVIENDEFVKNLEKHAINPYTKWWLEQ 479 Query: 479 NPYTFLGG 486 PY FL G Sbjct: 480 PPYYFLKG 487 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,011,995,917 Number of extensions: 125874091 Number of successful extensions: 291281 Number of sequences better than 1.0e-05: 797 Number of HSP's gapped: 289957 Number of HSP's successfully gapped: 797 Length of query: 493 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 353 Effective length of database: 1,741,754,103 Effective search space: 614839198359 Effective search space used: 614839198359 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)