BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0016.p (196 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LR28.1 A6LR28_CLOB8 Nitrogenase EC=1.18.6.1 228 2e-58 A5N6E8.1 A5N6E8_CLOK5 NifK1 EC=1.18.6.1 187 6e-46 B9DZW8.1 B9DZW8_CLOK1 Putative uncharacterized protein 187 6e-46 B9E0H8.1 B9E0H8_CLOK1 Putative uncharacterized protein 178 2e-43 A5N709.1 A5N709_CLOK5 NifK4 EC=1.18.6.1 178 2e-43 >A6LR28.1 A6LR28_CLOB8 Nitrogenase EC=1.18.6.1 Length = 442 Score = 228 bits (581), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 109/191 (57%), Positives = 143/191 (74%), Gaps = 5/191 (2%) Query: 2 SAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEER 61 S+W GL AE L+NKY TP+ HFPYLP+G I T +FLR++ +F +D+ E FI EE Sbjct: 219 SSWVGLNIAEHLKNKYKTPYVHFPYLPIGAIATSKFLRQIGEFGHLDKNKVENFIKKEEE 278 Query: 62 KFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTPE 121 KFY HIE+TADFMLEFRYGLPR FY++LDA+Y++GF+K+LLNELGILP + Q+++D+TPE Sbjct: 279 KFYFHIEKTADFMLEFRYGLPRKFYSILDASYSLGFSKFLLNELGILPGK-QFIIDDTPE 337 Query: 122 EYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQKKPKQ----RALILGSGWERDLAD 177 +Y+ I ++F IS RSA+VEF D GA+Q +I ++K K RALILGSGW+RDLA Sbjct: 338 QYKESIIKEFESISEYRSAEVEFNVDAGAVQLEIEKEKEKDFQEGRALILGSGWDRDLAA 397 Query: 178 ELGAXXXXQSV 188 +L A SV Sbjct: 398 KLNADLLITSV 408 >A5N6E8.1 A5N6E8_CLOK5 NifK1 EC=1.18.6.1 Length = 442 Score = 187 bits (474), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 89/182 (48%), Positives = 124/182 (68%), Gaps = 2/182 (1%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 +S W GL +A+LLE KYGTP+ H+P LP+G ET +FLR V DF+ +D+ AEAFI +EE Sbjct: 221 LSPWVGLNTAKLLEKKYGTPYLHYPILPIGAKETSKFLRTVGDFAKLDKEKAEAFINEEE 280 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 +FY +I+R+ DF +EFRY + F + D+ YA+G +K+L NELGI+P E QY+ D+TP Sbjct: 281 ERFYYYIDRSIDFFIEFRYSVADRFSNITDSFYALGISKFLTNELGIVPGE-QYITDDTP 339 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQ-KKPKQRALILGSGWERDLADEL 179 E+YR I +F +S+ SAKV F DGG I + +R+ + LILGS W+RD+ EL Sbjct: 340 EQYRKSIEHEFEHLSNSTSAKVIFEVDGGKIHQRLRKYEYGNHTPLILGSSWDRDIVSEL 399 Query: 180 GA 181 Sbjct: 400 NG 401 >B9DZW8.1 B9DZW8_CLOK1 Putative uncharacterized protein Length = 443 Score = 187 bits (474), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 89/182 (48%), Positives = 124/182 (68%), Gaps = 2/182 (1%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 +S W GL +A+LLE KYGTP+ H+P LP+G ET +FLR V DF+ +D+ AEAFI +EE Sbjct: 222 LSPWVGLNTAKLLEKKYGTPYLHYPILPIGAKETSKFLRTVGDFAKLDKEKAEAFINEEE 281 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 +FY +I+R+ DF +EFRY + F + D+ YA+G +K+L NELGI+P E QY+ D+TP Sbjct: 282 ERFYYYIDRSIDFFIEFRYSVADRFSNITDSFYALGISKFLTNELGIVPGE-QYITDDTP 340 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQ-KKPKQRALILGSGWERDLADEL 179 E+YR I +F +S+ SAKV F DGG I + +R+ + LILGS W+RD+ EL Sbjct: 341 EQYRKSIEHEFEHLSNSTSAKVIFEVDGGKIHQRLRKYEYGNHTPLILGSSWDRDIVSEL 400 Query: 180 GA 181 Sbjct: 401 NG 402 >B9E0H8.1 B9E0H8_CLOK1 Putative uncharacterized protein Length = 442 Score = 178 bits (452), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 88/182 (48%), Positives = 123/182 (67%), Gaps = 2/182 (1%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 VS W G+++AE L+ KYGTP+ H+ P+G ET +FLR V +F+++D + E FI +EE Sbjct: 221 VSPWVGIETAEFLKEKYGTPYLHYALPPIGAGETSKFLRTVGEFANLDFSRVEDFIKNEE 280 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 FY ++ER ADF LEFRY +P F+ + D+ YA+G +++LLNELGILP E QY+ D+TP Sbjct: 281 DNFYYYLERAADFFLEFRYSIPDRFFNITDSFYALGISQFLLNELGILPGE-QYITDDTP 339 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQKKPKQRA-LILGSGWERDLADEL 179 ++Y+ I +F KISS R +V F DGG I E +R+ LILGS W+RD+ EL Sbjct: 340 KKYQNDIINEFSKISSTRKFEVFFELDGGKIHERLRKYNLGMHIPLILGSSWDRDITREL 399 Query: 180 GA 181 A Sbjct: 400 NA 401 >A5N709.1 A5N709_CLOK5 NifK4 EC=1.18.6.1 Length = 442 Score = 178 bits (452), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 88/182 (48%), Positives = 123/182 (67%), Gaps = 2/182 (1%) Query: 1 VSAWPGLKSAELLENKYGTPFYHFPYLPVGGIETGRFLREVADFSSVDRAAAEAFILDEE 60 VS W G+++AE L+ KYGTP+ H+ P+G ET +FLR V +F+++D + E FI +EE Sbjct: 221 VSPWVGIETAEFLKEKYGTPYLHYALPPIGAGETSKFLRTVGEFANLDFSRVEDFIKNEE 280 Query: 61 RKFYAHIERTADFMLEFRYGLPRVFYTMLDATYAVGFAKYLLNELGILPAETQYVVDNTP 120 FY ++ER ADF LEFRY +P F+ + D+ YA+G +++LLNELGILP E QY+ D+TP Sbjct: 281 DNFYYYLERAADFFLEFRYSIPDRFFNITDSFYALGISQFLLNELGILPGE-QYITDDTP 339 Query: 121 EEYRAGIAEQFRKISSRRSAKVEFVEDGGAIQEDIRQKKPKQRA-LILGSGWERDLADEL 179 ++Y+ I +F KISS R +V F DGG I E +R+ LILGS W+RD+ EL Sbjct: 340 KKYQNDIINEFSKISSTRKFEVFFELDGGKIHERLRKYNLGMHIPLILGSSWDRDITREL 399 Query: 180 GA 181 A Sbjct: 400 NA 401 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,151,082,475 Number of extensions: 44114340 Number of successful extensions: 100418 Number of sequences better than 1.0e-05: 38 Number of HSP's gapped: 100368 Number of HSP's successfully gapped: 38 Length of query: 196 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 67 Effective length of database: 1,845,385,268 Effective search space: 123640812956 Effective search space used: 123640812956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)