BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0006.p (340 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIM3.1 B3KIM3_9BACI Cobalamin synthesis protein P47K 405 e-111 B1DH78.1 B1DH78_9BACL Cobalamin synthesis protein P47K 353 2e-95 Q9KFV5.1 Q9KFV5_BACHD BH0366 protein 200 2e-49 A6CHD0.1 A6CHD0_9BACI Possible GTPase 194 8e-48 C0ZHD3.1 C0ZHD3_BREBN Putative uncharacterized protein 186 3e-45 >B3KIM3.1 B3KIM3_9BACI Cobalamin synthesis protein P47K Length = 356 Score = 405 bits (1041), Expect = e-111, Method: Compositional matrix adjust. Identities = 200/352 (56%), Positives = 245/352 (69%), Gaps = 24/352 (6%) Query: 1 MEQAMPVYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMA 60 ME P+YILSGFLGSGKTTLL RL+ +K QGLRPA+VMNE+GEVN++GL +++VP A Sbjct: 1 MEHVTPIYILSGFLGSGKTTLLSRLIASFKQQGLRPAIVMNEIGEVNIEGLAADEAVPTA 60 Query: 61 EMLGGCICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELK 120 EML GCICC+IR DLS+E+A LI++E PDV+VIEATG ANP+EI+DA E SLY +++K Sbjct: 61 EMLSGCICCTIRSDLSSEIAMLIQRERPDVIVIEATGIANPMEILDATAEASLYMRIDVK 120 Query: 121 GLITVVDAAHLIELYRSQQGATYRLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWN 180 LITVVDAAHL+ L Q+G TYRLMQEQIR AS L+LNK D V ++ + I ++++WN Sbjct: 121 KLITVVDAAHLLHLNIEQKGKTYRLMQEQIRAASALVLNKMDLVEDRDRETIRELVKRWN 180 Query: 181 AYAEILPAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAV-------- 232 +A I+P+VRC L E LL+ G E E H A Sbjct: 181 PHASIVPSVRCDLNVEDLLQSDPGDRNEHSIVHHREAGDAHEHRDKNKQANSHEHDHSHS 240 Query: 233 ----------------HGSHDHVMAYTHYFKRPVNSEEFEQFVKELPRDVYRAKGIVTFS 276 HGSHDHVM+YT+YF PVNS EFE+ + LPRDVYRAKGI+TFS Sbjct: 241 HEHDHNHNHDHDQTHDHGSHDHVMSYTYYFSHPVNSVEFERLIAGLPRDVYRAKGILTFS 300 Query: 277 DTSSRFLFQYAYREADFMKITPQGDVPDVAVFIGEHFSSSELRTRLLQLEKR 328 DT+SRFLFQYA+RE+DFMKITPQG VPDV VFIGEHF S LR +L QLE + Sbjct: 301 DTNSRFLFQYAFRESDFMKITPQGQVPDVVVFIGEHFQRSLLREQLEQLEGK 352 >B1DH78.1 B1DH78_9BACL Cobalamin synthesis protein P47K Length = 331 Score = 353 bits (905), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 179/328 (54%), Positives = 234/328 (71%), Gaps = 14/328 (4%) Query: 5 MPVYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMAEMLG 64 +P+Y+LSGFLGSGKTTLL LL+ WKAQGL+ AV+MNELG++NLDG ++ VPM+EML Sbjct: 6 IPIYLLSGFLGSGKTTLLTGLLEQWKAQGLKAAVIMNELGDINLDGQMIGDKVPMSEMLS 65 Query: 65 GCICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLIT 124 GCICC+IR DLS E+ TLI PDV+VIE+TGAANP+EI+D +TE ++Y +EL+ +IT Sbjct: 66 GCICCTIRSDLSLEIKTLIDTHQPDVIVIESTGAANPMEIMDGITEAAMYCRIELQSVIT 125 Query: 125 VVDAAHLIELYRSQQGATYRLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYAE 184 VVD L++ RS +G TYRLM++QIRCA+ L+LNKTD++ P E E ++R+ NA A Sbjct: 126 VVDGPELLQRSRSGKGKTYRLMRDQIRCATRLLLNKTDKLAPDELVEAQQLIREINAVAP 185 Query: 185 ILPAVRCALEPEALLEGMGGIHAEARFQED-DEMQAGHAGDSSE----AGAAVHGSHDHV 239 + VRC +E A +E R ED ++ GH ++ A H +H+HV Sbjct: 186 VRTTVRCQMEDWAFIE---------RQAEDAPQLSGGHQEEAERKHGHHDAHHHHTHEHV 236 Query: 240 MAYTHYFKRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAYREADFMKITPQ 299 M THY V+SE FE F+K+LP VYRAKGIV+ SDT+SRFLFQYAYRE DF++ITPQ Sbjct: 237 MVMTHYMNGAVDSEAFEAFLKQLPDHVYRAKGIVSLSDTASRFLFQYAYRETDFIRITPQ 296 Query: 300 GDVPDVAVFIGEHFSSSELRTRLLQLEK 327 G+V DVAVFIGEHFS + L +LE+ Sbjct: 297 GEVNDVAVFIGEHFSKDAVLEELHKLER 324 >Q9KFV5.1 Q9KFV5_BACHD BH0366 protein Length = 311 Score = 200 bits (508), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 121/322 (37%), Positives = 186/322 (57%), Gaps = 19/322 (5%) Query: 7 VYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMAEMLGGC 66 +YILSGFLGSGK+TLL+RL++ +A+G + V+MNELGEV++D +V + P+ EML GC Sbjct: 8 LYILSGFLGSGKSTLLKRLIEQEQARGNKIGVLMNELGEVSVDSSIVPKDTPLKEMLNGC 67 Query: 67 ICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLITVV 126 ICC+I+G+LS +L + I++ DV+ IEATGAA+PLE+V+A T V ++G++TVV Sbjct: 68 ICCTIQGELSAQLQSFIEEYELDVIFIEATGAAHPLEVVEACTHPVFAGQVRVRGVLTVV 127 Query: 127 DAAHLIELYRSQQGATYRLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYAEIL 186 +A IE S + +LM+ Q+ A ++++NK D+VT + +++T ++ N A + Sbjct: 128 NARQWIEGKMSIK--IKKLMEAQVTFADLIVVNKVDQVTDAQVEQLTTSIKDLNPKAINI 185 Query: 187 PAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAVHGSHDHVMAYTHYF 246 P LEP +H+E R A + SE+ A VH H H+ Sbjct: 186 PTTFSNLEP---------VHSE-RLHH----AAAPSSRGSESKAHVH-EHLHLRTMAVPI 230 Query: 247 KRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAYREADFMKI-TPQGDVPDV 305 + PV+ E F+K + +YR KG + ++ FLF YAY + F + T Q P V Sbjct: 231 QHPVDRMELLSFLKGIQGQIYRVKGFIHLKESPGLFLFNYAYGDPMFERHRTDQAYAP-V 289 Query: 306 AVFIGEHFSSSELRTRLLQLEK 327 VFIG+ + L QL++ Sbjct: 290 LVFIGDDLDHQAIEEGLQQLQQ 311 >A6CHD0.1 A6CHD0_9BACI Possible GTPase Length = 309 Score = 194 bits (494), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 116/323 (35%), Positives = 177/323 (54%), Gaps = 25/323 (7%) Query: 7 VYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQSVPMAEMLGGC 66 VY+LSGFLGSGKTTLL+ L+ K + L+PAV+MNELG +++D VE+ P+ E+L GC Sbjct: 5 VYLLSGFLGSGKTTLLKNLITALKDKNLKPAVLMNELGSISIDSQEVEEGTPLKELLDGC 64 Query: 67 ICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLITVV 126 ICCSI+ L ++L L+ ++ DV+VIE TGAA+P+E+VDAV KG+ITVV Sbjct: 65 ICCSIQDKLESQLQELLFQDEFDVLVIETTGAAHPVEVVDAVMSPLFADRFNFKGIITVV 124 Query: 127 DAAHLIE--LYRSQQGATYRLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYAE 184 D + Y Q +LM+EQIR A +L+ NK D ++ E +++ N A+ Sbjct: 125 DGKQWVSRGAYSPQ---VLQLMREQIRHAHLLVFNKEDLLSEMEKGTSLFEIQQINPKAK 181 Query: 185 ILPAVRCALEPEALLEGMGGIHAEARFQEDDEMQAGHAGDSSEAGAAVHGSHDHVMAYTH 244 ++ + LL E +M S E + G ++ AY H Sbjct: 182 LIMTNYSKVSSSDLLN-----------LEGKQM-------SGEREKSAIGHQLNLRAYVH 223 Query: 245 YFKRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYAYREADFMKITPQGDVPD 304 F ++ +FE++++ LP +YR KG + FS F+FQY+Y + + ++P Sbjct: 224 RFTDSISQTDFEEWLRNLPDSIYRMKGYIPFSGRRYPFMFQYSYGMP--LLLPEDMNLPK 281 Query: 305 VAVFIGEHFSSSELRTRLLQLEK 327 V IGE ++L+T L +LE+ Sbjct: 282 TLVIIGEDLDENKLKTELQELER 304 >C0ZHD3.1 C0ZHD3_BREBN Putative uncharacterized protein Length = 338 Score = 186 bits (472), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 110/327 (33%), Positives = 177/327 (54%), Gaps = 31/327 (9%) Query: 7 VYILSGFLGSGKTTLLQRLLDHWKAQGLRPAVVMNELGEVNLDGLLVEQ-SVPMAEMLGG 65 VY+L+G+LGSGKTTLLQ+ L H + + V+MNE+GE ++DG ++ P+ +ML G Sbjct: 5 VYLLTGYLGSGKTTLLQKWLTHLRITDQKVVVLMNEMGEEDIDGEQLQGFGFPVKKMLDG 64 Query: 66 CICCSIRGDLSTELATLIKKESPDVVVIEATGAANPLEIVDAVTEISLYQLVELKGLITV 125 CICCSI+G+L+ L ++ +PD ++IE TG A+PL+++D +T LY +ELKG I+V Sbjct: 65 CICCSIKGELTEGLKDIMNTIAPDRILIETTGVADPLDVIDTITHPELYDRLELKGTISV 124 Query: 126 VDAAHLIELYR--SQQGATYRLMQEQIRCASVLILNKTDRVTPQEADEITVILRKWNAYA 183 VDA+ ++L S + ++ Q+R A +L+LNKTD +P + + L + N A Sbjct: 125 VDASRFLDLNSRFSSTATLVKTIRNQVRYADLLLLNKTDLTSPDVIERVRQKLSEINPTA 184 Query: 184 EILPAVRCALEPEALLEGMGGIHAEA----------------RFQEDDEMQAGHAGDSSE 227 I V+ +E LL +H + R D ++ +S+ Sbjct: 185 PIHATVQAKIEVSRLLSVKRTVHERSPGNEISRPIAVQKSIGRMPTMDRLKQSLGLKTSQ 244 Query: 228 AGAAVHGSHDHVMAYTHYFKRPVNSEEFEQFVKELPRDVYRAKGIVTFSDTSSRFLFQYA 287 ++ + +++ F PV++++FE F+ +LP++VYRAKG V F FQ+ Sbjct: 245 PSL-----YNSIETFSYVFTGPVDAKKFEDFLYDLPKNVYRAKGYVQFHGKPELISFQHT 299 Query: 288 YREA---DFMKITPQGDVPDVAVFIGE 311 + F P+ VAVFIGE Sbjct: 300 DNQVLLFPFENFGPKM----VAVFIGE 322 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,905,538,730 Number of extensions: 76303642 Number of successful extensions: 281068 Number of sequences better than 1.0e-05: 2299 Number of HSP's gapped: 273785 Number of HSP's successfully gapped: 2302 Length of query: 340 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 204 Effective length of database: 1,779,438,163 Effective search space: 363005385252 Effective search space used: 363005385252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)