BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0033.p (458 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL19.1 B3KL19_9BACI FolC bifunctional protein 648 0.0 B1DDS1.1 B1DDS1_9BACL FolC bifunctional protein 566 e-159 C0ZAJ3.1 C0ZAJ3_BREBN Folylpolyglutamate synthase EC=6.3.2.17 356 4e-96 A9VIT1.1 A9VIT1_BACWK FolC bifunctional protein 349 3e-94 Q633Y7.1 Q633Y7_BACCZ Folylpolyglutamate synthase EC=6.3.2.17 349 4e-94 >B3KL19.1 B3KL19_9BACI FolC bifunctional protein Length = 467 Score = 648 bits (1672), Expect = 0.0, Method: Compositional matrix adjust. Identities = 314/447 (70%), Positives = 359/447 (80%) Query: 12 PLTSYTEAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLT 71 P +Y EAV WINGLIPFGIRPG++RI+RLME LG P RRLKFIHVAGTNGKGSTCAFLT Sbjct: 21 PFHTYEEAVAWINGLIPFGIRPGMDRIQRLMELLGHPERRLKFIHVAGTNGKGSTCAFLT 80 Query: 72 SVLIRSGYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQTELGSPT 131 VL++ G++VGT+TSPYITKFTNRFQYNG DIPE L ELANRL PLV E+A + LGSPT Sbjct: 81 KVLLQGGFTVGTYTSPYITKFTNRFQYNGEDIPEETLLELANRLEPLVAEVAGSGLGSPT 140 Query: 132 MFEVATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTL 191 MFEV+TALAILYY EV YPDVVVWETGLGGRLDVTNIV PI+SVITN+G DHTDVLGDT+ Sbjct: 141 MFEVSTALAILYYGEVSYPDVVVWETGLGGRLDVTNIVHPIISVITNIGMDHTDVLGDTI 200 Query: 192 EAIAGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQ 251 + IA EKAGIIKPGVP V+ V+QPE E FSY Q Sbjct: 201 QEIAMEKAGIIKPGVPVVSTVTQPEAVEILKETAAKNRTSLYLVDEQFSYERLRGDEESQ 260 Query: 252 TFTFKGPFRSLELGIAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFWAG 311 TF FKGPFR ++L I+M+GEHQ +N+A AMM LEVLRQYMAFI+++E++ GF+ TFWAG Sbjct: 261 TFRFKGPFRDMDLDISMQGEHQCANAAVAMMTLEVLRQYMAFIVDEEELRAGFKDTFWAG 320 Query: 312 RLEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYDKLNLLMGMLANKHHESYFKHILPI 371 RLE+V +SPRIVLDGAHNPEGAESLAKSLPQ YKY KLNL+MGMLANKHHESY +HILPI Sbjct: 321 RLEQVQSSPRIVLDGAHNPEGAESLAKSLPQIYKYRKLNLMMGMLANKHHESYLQHILPI 380 Query: 372 VDTLILTEPDFRKKMDAEDLQAIAERLREKYAKENLVIKVEHDWGQALQLLQSITAEQDL 431 VDTLILTEPDFRKK+DA+ L I E++REKYAK L I +E +W AL+ L+S T +DL Sbjct: 381 VDTLILTEPDFRKKLDAKALGDIVEQVREKYAKHTLQIIIEPNWKNALEELKSRTEAEDL 440 Query: 432 AVVSGTLYLISDVRSTLLRQPNSEKGW 458 VVSGTLYLI+DVRS LL +SEKGW Sbjct: 441 GVVSGTLYLIADVRSNLLGLSDSEKGW 467 >B1DDS1.1 B1DDS1_9BACL FolC bifunctional protein Length = 458 Score = 566 bits (1459), Expect = e-159, Method: Compositional matrix adjust. Identities = 279/448 (62%), Positives = 335/448 (74%), Gaps = 5/448 (1%) Query: 13 LTSYTEAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTS 72 L SY EAVDWINGLIPFGIRPGL+RIE+L+E+L PHRRLKFIH+AGTNGKGSTCA+LTS Sbjct: 14 LRSYKEAVDWINGLIPFGIRPGLDRIEQLLERLNNPHRRLKFIHIAGTNGKGSTCAYLTS 73 Query: 73 VLIRSGYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQTELGSPTM 132 VL++ GY VGTFTSPYITKFTNRFQYNG DI E L L+N L VEE+A+TE GSPTM Sbjct: 74 VLMKCGYDVGTFTSPYITKFTNRFQYNGQDIDEETLLRLSNVLKSHVEELAETEFGSPTM 133 Query: 133 FEVATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTLE 192 FEV+TALAIL+YA V YPD VVWETGLGGR+DVTNIVTPI+SVITNVGHDH D LGDTLE Sbjct: 134 FEVSTALAILFYATVTYPDYVVWETGLGGRMDVTNIVTPIISVITNVGHDHMDRLGDTLE 193 Query: 193 AIAGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQT 252 A+A EKAGIIK GVP V+ V QPE G+ F+ QT Sbjct: 194 AVAFEKAGIIKAGVPVVSAVDQPEVVEVVKRTAAEKKSTLYLLGDQFNIKELSSEEDEQT 253 Query: 253 FTFKGPFRSLE-LGIAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFWAG 311 F F+G FRS++ L I + G HQ +N+A A+M LEVLRQY A I+ED+ + +G R W G Sbjct: 254 FRFEGMFRSIDPLTITLNGAHQRTNAAVAVMTLEVLRQYYALIVEDDALADGLREAKWPG 313 Query: 312 RLEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYDKLNLLMGMLANKHHESYFKHILPI 371 RLE VS SPRI++DGAHNPEGAESL ++L Y+YD+L+++M M+ NK+H KHILPI Sbjct: 314 RLEMVSRSPRILIDGAHNPEGAESLVQALRSTYRYDRLHVMMAMVENKNHRDVLKHILPI 373 Query: 372 VDTLILTEPDFRKKMDAEDLQAIAERLRE-KYAKENLVIKVEHDWGQALQLLQSITAEQD 430 VDTLI+TEPDFR K+DAE +A+ +R+ K A+ + + VE DW + L+ LQ +T E D Sbjct: 374 VDTLIVTEPDFRSKLDAE---LLADLVRDVKPAESSFELIVEKDWREGLRKLQQLTGETD 430 Query: 431 LAVVSGTLYLISDVRSTLLRQPNSEKGW 458 L VV+GTLYLI DVRS +L +SEKGW Sbjct: 431 LGVVTGTLYLIGDVRSRILYNSDSEKGW 458 >C0ZAJ3.1 C0ZAJ3_BREBN Folylpolyglutamate synthase EC=6.3.2.17 Length = 457 Score = 356 bits (913), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 201/447 (44%), Positives = 265/447 (59%), Gaps = 20/447 (4%) Query: 13 LTSYTEAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTS 72 YT+A+DW++GL+ FG RPGLER++ ++E++G P RR+ FIHVAGTNGKGSTCAFLT Sbjct: 7 FVEYTQALDWLHGLLRFGQRPGLERMQWVLEQVGHPERRIPFIHVAGTNGKGSTCAFLTQ 66 Query: 73 VLIRSGYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQ-TELGSPT 131 +L+ +GYSVGTFTSPY+ F R +YNG I E L +L N + V+ Q TE GSPT Sbjct: 67 MLMEAGYSVGTFTSPYLVDFRERIRYNGSMIAEEDLLQLVNEVKVWVQRCEQETEYGSPT 126 Query: 132 MFEVATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTL 191 FEV T +AILY++ V P VVVWETGLGGRLD TN+V P+VSVITN+G DHT VLG++L Sbjct: 127 EFEVITLIAILYFSRVARPAVVVWETGLGGRLDSTNVVLPLVSVITNIGMDHTQVLGESL 186 Query: 192 EAIAGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQ 251 E IA EKAGIIKPGVP VT +PE GE F G Q Sbjct: 187 EDIAREKAGIIKPGVPVVTGEVRPELLAVFEERARQMKSTLYHIGEHFQAEQVEEQLGDQ 246 Query: 252 TFTFKGPFRSLELG--IAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFW 309 F +K R E + M G HQ +N+A A+M ++VLR Y +F+LE+E++ G + T W Sbjct: 247 QFGYKSIHRRAEASYRLTMNGIHQTTNAAVALMTIDVLRNYFSFLLEEEEISRGLQQTRW 306 Query: 310 AGRLEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYD-KLNLLMGMLANKHHESYFKHI 368 GRLE +S P ++LDGAHN EG E+L SL + +L+LL LA+K + + Sbjct: 307 PGRLEVISPRPMVILDGAHNQEGMEALVSSLDRLLPDGVQLHLLFSGLADKPLAKMAESL 366 Query: 369 LPI---VDTLILTEPDFRKKMDAEDLQAIAERLREKYAK---ENLVIKVEHDWGQAL--- 419 P+ + L +T DF + +A LQ E + E + DW L Sbjct: 367 APVKSKMKKLHVTTFDFPRAAEANSLQ-------ESFLAGGWEQEFLDAVPDWRAFLLSW 419 Query: 420 QLLQSITAEQDLAVVSGTLYLISDVRS 446 + + D VV G+LY I+ VR+ Sbjct: 420 MREKQLAKADDWLVVCGSLYFIAQVRA 446 >A9VIT1.1 A9VIT1_BACWK FolC bifunctional protein Length = 433 Score = 349 bits (896), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 172/442 (38%), Positives = 271/442 (61%), Gaps = 11/442 (2%) Query: 13 LTSYTEAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTS 72 + +Y EA++WI+ + FGI+PGLER++ ++E+LG P R +K +H+AGTNGKGST ++ Sbjct: 2 IHTYEEAINWIHSRLKFGIKPGLERMQWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRY 61 Query: 73 VLIRSGYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQTELGSPTM 132 +L + Y VGTFTSPYI F R NG+ I + + EL N + P+VE++ +T+LG T Sbjct: 62 MLEGAKYKVGTFTSPYIETFNERISVNGIPIADEEIAELVNMVKPVVEKLDETDLGEATE 121 Query: 133 FEVATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTLE 192 FE+ T +AI Y+ +V + DVV++ETGLGGR D TN++ P++++ITN+GHDH +LGDTL Sbjct: 122 FEIITVMAIYYFGKVNFCDVVLFETGLGGRFDSTNVIHPVLTIITNIGHDHMHILGDTLG 181 Query: 193 AIAGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQT 252 IA EKAGIIK GVP +T V E G+ F+ + + Sbjct: 182 EIAYEKAGIIKSGVPVITGVKNEEALQVIQKVAKENHASLYELGKQFTILHKHSNKDGEH 241 Query: 253 FTFKGPFRSLE-LGIAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFWAG 311 F F PF S E + I+MKG HQ+ N+A A+MA+ L+ Y++F++ +E++ G + +W G Sbjct: 242 FDFDCPFASFENVRISMKGIHQVGNAALALMAVMYLKTYLSFLINEEEMKAGLQEAYWIG 301 Query: 312 RLEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYDKLNLLMGMLANKHHESYFKHILPI 371 R E++ ++P I++DGAHNPEG ESL K++ YK + +L L +K + + + Sbjct: 302 RFEKLQSNPDIIIDGAHNPEGIESLVKTVEAHYKKKNVIVLFTALGDKQLHNMVGQLEKV 361 Query: 372 VDTLILTEPDFRKKMDAEDLQAIAERLREKYAKENLVIKVEHDWGQALQLLQSITAEQDL 431 D +I T F + + A+ L + +++ + LV + DW +A+ + E D+ Sbjct: 362 ADEIIFTTFAFDRAISADKLASYSQQ------ESKLVFE---DWKEAIDTKADMIGEHDV 412 Query: 432 AVVSGTLYLISDVRSTLLRQPN 453 +++G+LY IS+VR +R+ N Sbjct: 413 FIITGSLYFISEVRK-YIREKN 433 >Q633Y7.1 Q633Y7_BACCZ Folylpolyglutamate synthase EC=6.3.2.17 Length = 433 Score = 349 bits (895), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 175/442 (39%), Positives = 266/442 (60%), Gaps = 11/442 (2%) Query: 13 LTSYTEAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTS 72 + +Y EA+ WI+ + FGI+PGLER+ ++E+LG P R +K +H+AGTNGKGST ++ Sbjct: 2 IHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRY 61 Query: 73 VLIRSGYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQTELGSPTM 132 +L + Y VGTFTSPYI F R NG I + +TEL + P+VE++ +T+LG T Sbjct: 62 MLEGAKYKVGTFTSPYIETFNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEATE 121 Query: 133 FEVATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTLE 192 FE+ T +AI Y+ +V + DVV++ETGLGGR D TN++ P++++ITN+GHDH +LG+TLE Sbjct: 122 FEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHPVLTIITNIGHDHMHILGNTLE 181 Query: 193 AIAGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQT 252 IA EKAGIIK GVP +T V E G F+ S + Sbjct: 182 EIAYEKAGIIKSGVPVITGVQDEEALQVIQKIAKENQANLYEMGNHFTSLHKQSSEDGEQ 241 Query: 253 FTFKGPFRSLE-LGIAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFWAG 311 F F PF S E + I MKG HQ+ N+A A+MA+ ++ Y++F++E+E++ G + +W G Sbjct: 242 FDFTCPFASFEDVRITMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWVG 301 Query: 312 RLEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYDKLNLLMGMLANKHHESYFKHILPI 371 R E++ ++P I++DGAHNPEG ESL K++ YK + +L L +K + + I Sbjct: 302 RFEKLQSNPDIIIDGAHNPEGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVDQLETI 361 Query: 372 VDTLILTEPDFRKKMDAEDLQAIAERLREKYAKENLVIKVEHDWGQALQLLQSITAEQDL 431 D +I T F + + A +L + A++ K EN W +A+ + E D+ Sbjct: 362 ADEIIFTTFAFDRAISAGELASYAKK-ESKLVFEN--------WKEAIDTKVEMIGENDV 412 Query: 432 AVVSGTLYLISDVRSTLLRQPN 453 +++G+LY IS+VR +R+ N Sbjct: 413 FIITGSLYFISEVRK-YIREKN 433 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,613,678,220 Number of extensions: 104038406 Number of successful extensions: 284180 Number of sequences better than 1.0e-05: 1890 Number of HSP's gapped: 279361 Number of HSP's successfully gapped: 2141 Length of query: 458 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 319 Effective length of database: 1,751,175,118 Effective search space: 558624862642 Effective search space used: 558624862642 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)