BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0032.p (452 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DDS0.1 B1DDS0_9BACL UDP-N-acetylmuramate--alanine ligase 674 0.0 B3KL20.1 B3KL20_9BACI UDP-N-acetylmuramate--alanine ligase 634 e-180 C0ZAJ4.1 MURC_BREBN UDP-N-acetylmuramate--L-alanine ligase EC=6.... 562 e-158 C1PTP1.1 C1PTP1_ALIAC UDP-N-acetylmuramate--alanine ligase EC=6.... 540 e-151 B7DU10.1 B7DU10_9BACL UDP-N-acetylmuramate/alanine ligase 538 e-151 >B1DDS0.1 B1DDS0_9BACL UDP-N-acetylmuramate--alanine ligase Length = 446 Score = 674 bits (1739), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/444 (73%), Positives = 378/444 (85%), Gaps = 5/444 (1%) Query: 11 MSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADLVVYSTALASD 70 MSAIARVMLEMGY +TGSDVA QELTEKL AKGA++YIGH AE VKGADLVVYSTAL+ D Sbjct: 1 MSAIARVMLEMGYKITGSDVARQELTEKLAAKGAQIYIGHEAEHVKGADLVVYSTALSKD 60 Query: 71 NVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIMEECGVDPTYI 130 NVE + AE LNIPVLHR+QMLARL+N KGVA+AGAHGKTTTSSMIAL+ME+C DPTYI Sbjct: 61 NVERMAAEELNIPVLHRAQMLARLMNAGKGVAVAGAHGKTTTSSMIALVMEKCDADPTYI 120 Query: 131 IGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLENYGGDFGRLK 190 IGGEI+NVGTNAKAG+GEYVVAEADESDGSFLQYHP + IVTNIE DHLENY GDFG+LK Sbjct: 121 IGGEIVNVGTNAKAGKGEYVVAEADESDGSFLQYHPSIAIVTNIEPDHLENYDGDFGKLK 180 Query: 191 AAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGN----VITYGIHSPSADYTATDIVLG 246 AAYV+F++Q++E+G AIVCADDET LLP++ + +ITYGI S S +Y A +I LG Sbjct: 181 AAYVKFLSQVKENGKAIVCADDETITELLPKLDADTASKLITYGIDSDS-EYKAENISLG 239 Query: 247 DRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESILKFHGAKR 306 DR+V++ M LG +ELS+PG++N+YN+MA VI CL+AG+ F IA++I +F GAKR Sbjct: 240 DRKVSFEMTHRGTTLGTLELSVPGRHNVYNAMATVITCLEAGLSFDNIAKAIHEFIGAKR 299 Query: 307 RFQVLGEANDILVIDDYAHHPTEIQATISAAKATGKRIIAVFQPQRYTRTFFLLDAFSRA 366 RFQVLGE NDILVIDDYAHHPTEIQATISAAKATGKRIIAVFQPQRYTRTFFLL+ FSRA Sbjct: 300 RFQVLGEVNDILVIDDYAHHPTEIQATISAAKATGKRIIAVFQPQRYTRTFFLLEQFSRA 359 Query: 367 FSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVLADLQDRIAP 426 F EADEVI+TDIYSPAGE+QIEGV S KLVE+I NSNAN ++PTK+ VL L ++AP Sbjct: 360 FPEADEVIITDIYSPAGEQQIEGVHSRKLVEMIKTNSNANTSYIPTKEEVLVHLTAKVAP 419 Query: 427 GDLVITMGAGDIWKVGYALAEGLK 450 GDLVITMGAGDIWK ALA+ LK Sbjct: 420 GDLVITMGAGDIWKTADALAKTLK 443 >B3KL20.1 B3KL20_9BACI UDP-N-acetylmuramate--alanine ligase Length = 393 Score = 634 bits (1635), Expect = e-180, Method: Compositional matrix adjust. Identities = 302/385 (78%), Positives = 346/385 (89%), Gaps = 1/385 (0%) Query: 66 ALASDNVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIMEECGV 125 AL+ DNVE VEAE+LNIPVLHR+QMLARLLNERKGVA+AGAHGKTTTSSMIAL+MEECGV Sbjct: 7 ALSKDNVERVEAEKLNIPVLHRAQMLARLLNERKGVAVAGAHGKTTTSSMIALVMEECGV 66 Query: 126 DPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLENYGGD 185 DPT+IIGGEIMNVGTNAKAG+GE+VVAEADESDGSFLQYHPWLG+V NIEADHLENY GD Sbjct: 67 DPTFIIGGEIMNVGTNAKAGEGEHVVAEADESDGSFLQYHPWLGVVLNIEADHLENYEGD 126 Query: 186 FGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYTATDIVL 245 F RLKAAYVQF++Q++ DGTA+VCADD ++LP++K NV TYGIH+ A+YTAT++VL Sbjct: 127 FNRLKAAYVQFLSQIKADGTAVVCADDNNIQAILPDLKCNVTTYGIHA-DAEYTATNLVL 185 Query: 246 GDRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESILKFHGAK 305 GDR V++TM LG +ELS+PGK+N+YN+MA VI CLK+GI F I E+I+KF+GAK Sbjct: 186 GDRHVSFTMLHNGMELGTVELSVPGKHNVYNAMATVICCLKSGISFGAIVEAIVKFNGAK 245 Query: 306 RRFQVLGEANDILVIDDYAHHPTEIQATISAAKATGKRIIAVFQPQRYTRTFFLLDAFSR 365 RRFQVLGEA ILVIDDYAHHPTEI+ATISAAKATGKRIIAVFQPQRY+RTFFLLDAFSR Sbjct: 246 RRFQVLGEAEGILVIDDYAHHPTEIEATISAAKATGKRIIAVFQPQRYSRTFFLLDAFSR 305 Query: 366 AFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVLADLQDRIA 425 AF EADEVI+TDIYSPAGEKQIEG+SS KLVELIVQNSNA AR+LPTK+ VL+DL+ R+ Sbjct: 306 AFGEADEVIITDIYSPAGEKQIEGISSGKLVELIVQNSNAGARYLPTKEDVLSDLKGRVK 365 Query: 426 PGDLVITMGAGDIWKVGYALAEGLK 450 GDLV+TMGAGDIWKVG ALAE +K Sbjct: 366 EGDLVLTMGAGDIWKVGAALAEFIK 390 >C0ZAJ4.1 MURC_BREBN UDP-N-acetylmuramate--L-alanine ligase EC=6.3.2.8 Length = 460 Score = 562 bits (1448), Expect = e-158, Method: Compositional matrix adjust. Identities = 264/454 (58%), Positives = 350/454 (77%), Gaps = 3/454 (0%) Query: 1 VHFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADL 60 VHF+GIGGYGMSAIARV++++GY VTGSDVA +L +KL A+GA ++IGH A V+GAD Sbjct: 7 VHFVGIGGYGMSAIARVLIDLGYKVTGSDVAMNDLAKKLEARGAVIHIGHNAAHVEGADS 66 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIM 120 +VYST+++ DN E + + L IPV+HRSQMLAR+LNER GVA+AGAHGKTTT+SMI+ ++ Sbjct: 67 IVYSTSISKDNPEVIAGQALGIPVMHRSQMLARILNERTGVAVAGAHGKTTTTSMISHVL 126 Query: 121 EECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLE 180 EECGVDPT+IIGGE+M+ GTNAKAG +YV+AEADESDGSFL+Y P IVT+IE DHLE Sbjct: 127 EECGVDPTFIIGGELMSAGTNAKAGTSDYVIAEADESDGSFLEYRPMYEIVTSIEPDHLE 186 Query: 181 NYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHS---PSAD 237 ++ GDF LK AYVQF++ ++ DG AI+C DD ++P V+ V+TY + A Sbjct: 187 HFDGDFNNLKQAYVQFLSHLKPDGKAILCIDDSHVREVMPSVEKTVVTYAVDPNFVAQAQ 246 Query: 238 YTATDIVLGDRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAES 297 ++AT I GDR + LG + L +PGK+N+ N++AA++ CL G+ F IA++ Sbjct: 247 FSATSIECGDRCSTCQVYHKDERLGELCLVVPGKHNIANALAAIVVCLDIGLSFDKIAQA 306 Query: 298 ILKFHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKATGKRIIAVFQPQRYTRTF 357 + F GAKRRFQ++G+A +LV+DDYAHHPTEI AT+S A+A+G+R+IAVFQPQRYTRT+ Sbjct: 307 LSTFSGAKRRFQIIGDARGVLVVDDYAHHPTEIIATLSGARASGRRVIAVFQPQRYTRTY 366 Query: 358 FLLDAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVL 417 FL+D FSRAF+EADEVI+TDIYSPAGE++IEGV+S LV+ I NS+ A+++PTK+ + Sbjct: 367 FLMDEFSRAFAEADEVIITDIYSPAGEEKIEGVTSEVLVQKIRTNSHPRAQYIPTKEQMQ 426 Query: 418 ADLQDRIAPGDLVITMGAGDIWKVGYALAEGLKK 451 + + GDLV+TMGAGDIWK Y LAE L+K Sbjct: 427 EHVFKELQEGDLVLTMGAGDIWKSAYWLAEKLEK 460 >C1PTP1.1 C1PTP1_ALIAC UDP-N-acetylmuramate--alanine ligase EC=6.3.2.8 Length = 463 Score = 540 bits (1391), Expect = e-151, Method: Compositional matrix adjust. Identities = 252/446 (56%), Positives = 338/446 (75%), Gaps = 1/446 (0%) Query: 1 VHFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADL 60 VHF+GIGGYGMSAIARVML++GY ++GSDV+ ELTEKL ++GA VY GH E V+GAD+ Sbjct: 7 VHFVGIGGYGMSAIARVMLDLGYQISGSDVSRHELTEKLASRGATVYYGHRPEHVEGADI 66 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIM 120 VV+STA+ DNVE A LNIPV+HRS+MLARL+++R G+A+ GAHGKTTT+SMIA M Sbjct: 67 VVHSTAVKQDNVELQAARALNIPVIHRSEMLARLMDDRVGIAVTGAHGKTTTTSMIAFAM 126 Query: 121 EECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLE 180 E+ G+DPT+I+GG + ++G NAKAG+G +VVAEADESDGSFL Y P + +VTN+E DHLE Sbjct: 127 EQNGLDPTFIVGGVVADLGDNAKAGKGPFVVAEADESDGSFLHYRPAIAVVTNVEPDHLE 186 Query: 181 NYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYTA 240 +Y G F L AY F+ Q+ +G ++ ADD+ L + V+TYG + AD TA Sbjct: 187 HYDGKFENLVRAYETFIRQIPSEGLLVMSADDDKLRELRSAARCRVVTYGF-AEDADITA 245 Query: 241 TDIVLGDRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESILK 300 + LGDR + +G ++L++PGK+N+ N++AA+ C++ G+ F+ A ++ + Sbjct: 246 RHVELGDRASRSDVLVNGKFVGTLQLALPGKHNVLNALAAIAVCMEVGLSFEAAAGALSR 305 Query: 301 FHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKATGKRIIAVFQPQRYTRTFFLL 360 FHGAKRRFQV+ E D+L++DDYAHHPTEI+ATI+AAK+TG+RI+AVFQPQRYTRTFFL Sbjct: 306 FHGAKRRFQVVAEVGDVLIVDDYAHHPTEIRATIAAAKSTGRRIVAVFQPQRYTRTFFLF 365 Query: 361 DAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVLADL 420 + F++AF EADEVIL DIYSPAGE++I+GVS+A+L I + SN + R++PT D V+ L Sbjct: 366 EEFAKAFGEADEVILCDIYSPAGERKIDGVSAARLASEIARMSNPHTRYIPTHDDVIEYL 425 Query: 421 QDRIAPGDLVITMGAGDIWKVGYALA 446 + PGDLV+TMGAGD+WKV LA Sbjct: 426 VQSVRPGDLVLTMGAGDVWKVAKGLA 451 >B7DU10.1 B7DU10_9BACL UDP-N-acetylmuramate/alanine ligase Length = 463 Score = 538 bits (1385), Expect = e-151, Method: Compositional matrix adjust. Identities = 252/446 (56%), Positives = 337/446 (75%), Gaps = 1/446 (0%) Query: 1 VHFIGIGGYGMSAIARVMLEMGYTVTGSDVAAQELTEKLIAKGAKVYIGHTAEQVKGADL 60 VHF+GIGGYGMSAIARVML++GY V+GSDV+ ELTEKL ++GA VY GH E V+GAD+ Sbjct: 7 VHFVGIGGYGMSAIARVMLDLGYRVSGSDVSRHELTEKLASRGATVYYGHRPEHVEGADI 66 Query: 61 VVYSTALASDNVEWVEAERLNIPVLHRSQMLARLLNERKGVAIAGAHGKTTTSSMIALIM 120 VV+STA+ DNVE A LNIPV+HRS+MLARL+++R G+A+ GAHGKTTT+SMIA M Sbjct: 67 VVHSTAVKQDNVELQAARALNIPVIHRSEMLARLMDDRVGIAVTGAHGKTTTTSMIAFTM 126 Query: 121 EECGVDPTYIIGGEIMNVGTNAKAGQGEYVVAEADESDGSFLQYHPWLGIVTNIEADHLE 180 E+ G+DPT+I+GG + ++G NAKAG+G +VVAEADESDGSFL Y P + +VTN+E DHLE Sbjct: 127 EQNGLDPTFIVGGVVADLGDNAKAGKGPFVVAEADESDGSFLHYRPAIAVVTNVEPDHLE 186 Query: 181 NYGGDFGRLKAAYVQFMNQMQEDGTAIVCADDETAASLLPEVKGNVITYGIHSPSADYTA 240 +Y G F L AY F+ Q+ +G ++ ADD+ L + V+TYG + AD TA Sbjct: 187 HYDGKFENLVRAYETFIRQIPSEGLLVMSADDDKLRELRSAARCRVVTYGF-AEDADITA 245 Query: 241 TDIVLGDRQVAYTMNFGSAVLGRIELSIPGKYNLYNSMAAVIACLKAGIPFQGIAESILK 300 + LGDR + +G ++L++PGK+N+ N++AA+ C++ G+ F+ A ++ + Sbjct: 246 RHVELGDRASRSDVLVNGEFVGTLQLALPGKHNVLNALAAIAVCMEVGLSFEAAAGALSR 305 Query: 301 FHGAKRRFQVLGEANDILVIDDYAHHPTEIQATISAAKATGKRIIAVFQPQRYTRTFFLL 360 FHGAKRRFQV+ E +L++DDYAHHPTEI+ATI+AAK+TG+RI+AVFQPQRYTRTFFL Sbjct: 306 FHGAKRRFQVVAEMGGVLIVDDYAHHPTEIRATIAAAKSTGRRIVAVFQPQRYTRTFFLF 365 Query: 361 DAFSRAFSEADEVILTDIYSPAGEKQIEGVSSAKLVELIVQNSNANARHLPTKDAVLADL 420 + F++AF EADEVIL DIYSPAGE++I+GVS+A+L I + SN + R++PT D V+ L Sbjct: 366 EEFAKAFGEADEVILCDIYSPAGERKIDGVSAARLASEIARMSNPHTRYIPTHDDVIEYL 425 Query: 421 QDRIAPGDLVITMGAGDIWKVGYALA 446 + PGDLV+TMGAGD+WKV LA Sbjct: 426 AQNVRPGDLVLTMGAGDVWKVAKGLA 451 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,590,298,262 Number of extensions: 104835306 Number of successful extensions: 316844 Number of sequences better than 1.0e-05: 4009 Number of HSP's gapped: 308419 Number of HSP's successfully gapped: 4083 Length of query: 452 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 313 Effective length of database: 1,751,175,118 Effective search space: 548117811934 Effective search space used: 548117811934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)