BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0020.p (286 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL30.1 B3KL30_9BACI Peptidase M23B 235 4e-60 B1DDQ9.1 B1DDQ9_9BACL Peptidase M23B 167 9e-40 A6CTK4.1 A6CTK4_9BACI Stage IV sporulation protein FA 87 2e-15 A7GTC7.1 A7GTC7_BACCN Peptidase M23B 78 1e-12 Q65GM1.1 Q65GM1_BACLD SpoIVFA 78 1e-12 >B3KL30.1 B3KL30_9BACI Peptidase M23B Length = 302 Score = 235 bits (599), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 134/306 (43%), Positives = 179/306 (58%), Gaps = 24/306 (7%) Query: 1 MEQKSGIKGRRKERIRSLLE------LDDSPH-TAVP---PLYALSDRTTTFKEWGEGAA 50 M+ K+ +K RR+ RIR LLE DD P T +P P + + K+ + Sbjct: 1 MDIKTSVKNRREARIRELLEGEQFTAQDDKPSWTGLPDKTPANPHNPKPGFIKDNIANPS 60 Query: 51 HDYSTDREPDPEALWKQRRGGWVDE----GGGDKPR---FASGFMRRTIASIVVFGAVWG 103 Y+ + EPDPE LWKQ GW D G+ PR F SG +RR S +VFG WG Sbjct: 61 PRYA-EHEPDPERLWKQGHRGWYDTLDPGAPGEPPRKASFLSGLIRRVAVSALVFGLAWG 119 Query: 104 IFAIQQPWALKVQAFITDALSRDMDFAAAQVWYEEHFSGAPAFIPIFGGEDQPAEKVTAA 163 +F+ QQPWAL+ QAFI + LS +MDF AAQVWYEEHF AP+FIPIFG D+ + KV A Sbjct: 120 VFSFQQPWALRAQAFIIEGLSHEMDFQAAQVWYEEHFGSAPSFIPIFGQTDEHSTKVNAG 179 Query: 164 HGLSTPLAGSIVRPFAATMNGVEIMPAEASNGTL---TVKSVDTGRVLSVAKEAQGGIRI 220 L PL+G +V+ FA + G+ + EA G+L VKS++TGRVL V + GI + Sbjct: 180 TSLVPPLSGRVVQSFAVDLKGIVL---EAGGGSLADRAVKSIETGRVLEVKEHPDNGITV 236 Query: 221 TVRHTGDITAEYGHLSGTKLAADDWVQSGDAVGWLEETENASVPLLFFAIMKDKTYIDPA 280 ++HTG+ TA Y L+ T L +DWVQ GD +G L + S P L+F + + +DPA Sbjct: 237 RIQHTGERTAAYSRLAETGLKVNDWVQGGDIIGTLASSGTGSPPSLYFELKEGDRDVDPA 296 Query: 281 EVVSFD 286 EV+ FD Sbjct: 297 EVIPFD 302 >B1DDQ9.1 B1DDQ9_9BACL Peptidase M23B Length = 326 Score = 167 bits (423), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 108/330 (32%), Positives = 161/330 (48%), Gaps = 48/330 (14%) Query: 1 MEQKSGIKGRRKERIRSLLELDD-----------SPHTAVPP--LYALSDRTTTFKEWGE 47 M +K GI+ RR+E+IR LLE + V P + + ++ E Sbjct: 1 MSKKDGIRERRQEKIRQLLEQQERQAKLWQQQERKQEQQVMPAKVRPVKEKQNPRYEEQS 60 Query: 48 GAAHDYSTDR----EPDPEALWKQRRGGW-----------------------VDEGGGDK 80 Y D E DPE LWK W ++EGGG + Sbjct: 61 FLPVPYHMDTGGGDELDPEKLWKTNPHPWLGWESGDGKNGRHGGDIASIYESINEGGGRR 120 Query: 81 PRFASGFMRRTIASIVVFGAVWGIFAIQQPWALKVQAFITDALSRDMDFAAAQVWYEEHF 140 P F S + I + ++F A+WG+F + P A K Q + +AL+ +MDF A WY+E F Sbjct: 121 PFFRSELKWKVIIAALLFAAIWGVFHNESPLAQKGQTIVKNALTDEMDFTAVATWYKETF 180 Query: 141 SGAPAFIPIFGGEDQPAEKVTAAHGLS--TPL-AGSIVRPFAATMNGVEIMPAEASNGTL 197 +GAP+FIPIF + AE + L +PL +G+IVR FA +NGVE+ A Sbjct: 181 AGAPSFIPIFNHNTKEAESANGSVELPVVSPLPSGAIVRTFAELLNGVEL----AGTSKE 236 Query: 198 TVKSVDTGRVLSVAKEAQG-GIRITVRHTGDITAEYGHLSGTKLAADDWVQSGDAVGWLE 256 V +++TGRVL V++ G G + + H + YG L + +DWV++GD +G L+ Sbjct: 237 EVSAIETGRVLVVSEAENGSGFTVVIEHANGRDSVYGKLGAANVKQNDWVEAGDGIGTLK 296 Query: 257 ETENASVPLLFFAIMKDKTYIDPAEVVSFD 286 ET + LL+FAI ++ Y+DP V+ D Sbjct: 297 ETASEEPSLLYFAIRQNNQYVDPVGVIPID 326 >A6CTK4.1 A6CTK4_9BACI Stage IV sporulation protein FA Length = 228 Score = 87.0 bits (214), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 54/216 (25%), Positives = 106/216 (49%), Gaps = 10/216 (4%) Query: 75 EGG---GDKPRFASG-FMRRTIASIVVFGAVWGIFAIQQPWALKVQAFITDALSRDMDFA 130 EGG G P F+ F+ + + + V+ +F Q P K ++++ +S + FA Sbjct: 19 EGGPSEGGHPLFSKEIFILKILGTSVIVLCTAILFQNQVPGFDKARSYVEKTMSNEFQFA 78 Query: 131 AAQVWYEEHFSGAPAFIPIFGGEDQPAEKVTAAHGLSTPLAGSIVRPFAATMNGVEIMPA 190 A WYE+ F A +P G++ T + P +G ++ F + G+ + Sbjct: 79 AISTWYEDQFGKPLALLP--AGKESSETGTTETAEYALPASGKVIENFKSNGQGIMVETG 136 Query: 191 EASNGTLTVKSVDTGRVLSVAKEAQGGIRITVRHTGDITAEYGHLSGTKLAADDWVQSGD 250 + S V+++ G V+ K+A G + ++H + YGHL+ ++ + V++G+ Sbjct: 137 KGSK----VEAMKGGLVIFAGKKADLGQTVIIQHDDKSESWYGHLNTIEVTQYETVKAGN 192 Query: 251 AVGWLEETENASVPLLFFAIMKDKTYIDPAEVVSFD 286 VG + + ++ + +FAI K+ +IDP +V+SF+ Sbjct: 193 PVGTVTDEQDTATGEFYFAIKKEDAFIDPIQVISFE 228 >A7GTC7.1 A7GTC7_BACCN Peptidase M23B Length = 247 Score = 77.8 bits (190), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 49/200 (24%), Positives = 89/200 (44%), Gaps = 9/200 (4%) Query: 87 FMRRTIASIVVFGAVWGIFAIQQPWALKVQAFITDALSRDMDFAAAQVWYEEHFSGAPAF 146 F+ + + + ++ +V +F P + +T + + FAA WYE HF AF Sbjct: 57 FLFKVLLAAILVLSVAILFKNAPPSFDGARVVVTKVMKEEFQFAAVSKWYEAHFGKPLAF 116 Query: 147 IPIFGGEDQPAEKVTAAHGLSTPLAGSIVRPFAATMNGVEIMPAEASNGTLTVKSVDTGR 206 +P ++ T + P +G + F GV + + TV+SV+ G Sbjct: 117 LPA-----SEKKQNTQQKNYAVPASGKVTEGFQKNGQGVLVQTVTGA----TVESVNEGV 167 Query: 207 VLSVAKEAQGGIRITVRHTGDITAEYGHLSGTKLAADDWVQSGDAVGWLEETENASVPLL 266 V+ K+ G I ++H + YG+L + D+V+ +G + + Sbjct: 168 VIFAGKKEGLGNTIEIQHADGTESWYGNLGEMSVKLYDYVEKKQKIGTVSNDDKNKNGKF 227 Query: 267 FFAIMKDKTYIDPAEVVSFD 286 +FAI K++ +IDP +V+SFD Sbjct: 228 YFAIKKNEKFIDPIQVISFD 247 >Q65GM1.1 Q65GM1_BACLD SpoIVFA Length = 263 Score = 77.8 bits (190), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 44/175 (25%), Positives = 87/175 (49%), Gaps = 10/175 (5%) Query: 112 ALKVQAFITDALSRDMDFAAAQVWYEEHFSGAPAFIPIFGGEDQPAEKVTAAHGLSTPLA 171 A +++ I+ + + FAA Q WYE F AF G +P+ +V L+ P Sbjct: 99 ANQIKPVISQVFTEEFQFAALQNWYESKFGDPLAFFQPKGA--KPSGQVEVNQDLAVPAV 156 Query: 172 GSIVRPFAATMNGVEIMPAEASNGTLTVKSVDTGRVLSVAKEAQGGIRITVRHTGDITAE 231 G + F+ G+++ + T++S+ G V+ V K + G+ + ++H + Sbjct: 157 GKVQEKFSG--QGIKVETEDE-----TIRSMKEGYVIEVDKNPETGLTVVLQHADNSYTY 209 Query: 232 YGHLSGTKLAADDWVQSGDAVGWLEETENASVPLLFFAIMKDKTYIDPAEVVSFD 286 YG L +A D++ G +G +E+ +N + +FAI + + ++DP +V++F+ Sbjct: 210 YGQLKKADVALYDYIDKGTKLGTIEQDKNQK-GIYYFAIKQGEEFVDPIQVITFE 263 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,800,788,664 Number of extensions: 77313245 Number of successful extensions: 189936 Number of sequences better than 1.0e-05: 94 Number of HSP's gapped: 190010 Number of HSP's successfully gapped: 94 Length of query: 286 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 152 Effective length of database: 1,798,280,193 Effective search space: 273338589336 Effective search space used: 273338589336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)