BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0019.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL31.1 B3KL31_9BACI Peptidase M50 295 2e-78 B1DDQ8.1 B1DDQ8_9BACL Peptidase M50 233 2e-59 C0ZAL0.1 C0ZAL0_BREBN Stage IV sporulation protein FB EC=3.4.24.- 144 6e-33 C2ZUW5.1 C2ZUW5_BACCE Stage IV sporulation protein FB 139 2e-31 C2ZDD1.1 C2ZDD1_BACCE Stage IV sporulation protein FB 139 2e-31 >B3KL31.1 B3KL31_9BACI Peptidase M50 Length = 287 Score = 295 bits (756), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 148/278 (53%), Positives = 192/278 (69%) Query: 1 LIRIRGIQLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKSVQ 60 +I+ +G L+LHPLFV++ML SV TG+FLELLTLF IVFIHE+GH AA G +V+++Q Sbjct: 1 MIKFKGTVLSLHPLFVIVMLASVFTGRFLELLTLFIIVFIHELGHAAAAAAMGFKVRAIQ 60 Query: 61 LLPFGGVAVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQAN 120 +LPFGGVAV+ED GR+TA++E+ IA+AGPLQNV++I + + G+G L Y IQ N Sbjct: 61 MLPFGGVAVIEDDGRMTAMKEIIIALAGPLQNVIMIGITLACQWAGWGDEQLLSYIIQGN 120 Query: 121 AIIALFNLLPVLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXXXX 180 +IALFNLLP+LPLDGGK+ QA+ W R I S++++GY + Sbjct: 121 LVIALFNLLPILPLDGGKVLQAVISLLAPYHATLLWASRAGILCSLIMIGYGLQPLFSGG 180 Query: 181 XXXXXXXMIGAFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAKPL 240 MIG FL YSN D RN+ YRF+ FL+NR VYER A+PIVA S+KPL Sbjct: 181 GVRLNVLMIGLFLAYSNFEDYRNVHYRFLRFLVNRSEVYERNLDGLGPAQPIVADSSKPL 240 Query: 241 DDILRLFKRNQYHFIYVMNEDKSIVAVVPEQKLISSYF 278 DDI+RLFKR +YH IYVM+ S++AVVPEQ +ISSYF Sbjct: 241 DDIMRLFKREKYHLIYVMSGRGSLLAVVPEQHVISSYF 278 >B1DDQ8.1 B1DDQ8_9BACL Peptidase M50 Length = 293 Score = 233 bits (593), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 122/278 (43%), Positives = 172/278 (61%), Gaps = 1/278 (0%) Query: 1 LIRIRGIQLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKSVQ 60 +I+ +GI+ ++HPLFVL+M+ SVVTG F EL TLF IV +HE+GHV A + V+ V+ Sbjct: 1 MIKWKGIRWSVHPLFVLVMIGSVVTGYFAELFTLFLIVLVHEIGHVVVARAFDLTVREVK 60 Query: 61 LLPFGGVAVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQAN 120 LLPFGGVA +ED G + A ++ IAIAGPLQNV + + A+G Q G+ + Y QAN Sbjct: 61 LLPFGGVAEIEDAGSIPAKQDSLIAIAGPLQNVWMGLAAWGCGQLGWWDPEWAAYVWQAN 120 Query: 121 AIIALFNLLPVLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXXXX 180 +I LFNLLP+ PLDGGK+ QA W +SI+ S+L++GYA+ Sbjct: 121 LMIGLFNLLPIYPLDGGKLMQAAFSYGFNYYSMMKWTSWISISLSMLMIGYALSNAFRPD 180 Query: 181 XXXXXXXM-IGAFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAKP 239 + +G FLL +N T RN+PY FV FLM+R+ V R G+ ARPI+ +P Sbjct: 181 AGIQLNLLVVGLFLLMTNWTYYRNMPYLFVRFLMHRDRVAVRAISRGTLARPIIVNLKQP 240 Query: 240 LDDILRLFKRNQYHFIYVMNEDKSIVAVVPEQKLISSY 277 + ++RL R QYH Y+M + +V VVPE++++ Y Sbjct: 241 IIGVVRLLWREQYHLFYLMELEGRVVKVVPEKEIVDRY 278 >C0ZAL0.1 C0ZAL0_BREBN Stage IV sporulation protein FB EC=3.4.24.- Length = 278 Score = 144 bits (364), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 96/271 (35%), Positives = 142/271 (52%), Gaps = 15/271 (5%) Query: 7 IQLALHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKSVQLLPFGG 66 I+ +H LF +++ LSVVTG FLE++TLF IV IHE+GHV A G VK VQLLPFGG Sbjct: 9 IRFRIHLLFWVVIGLSVVTGHFLEVITLFVIVLIHELGHVAMARELGWTVKEVQLLPFGG 68 Query: 67 VAVMEDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQANAIIALF 126 VA MED + E+ +A+AGP N++++ +Y G T + +F+ +N IALF Sbjct: 69 VATMEDSYATDPLDEIVVALAGPFLNMVMMAASYLFWFMGIWTEEWARFFLVSNLTIALF 128 Query: 127 NLLPVLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXXXXXXXXXX 186 NLLP+ PLDGG+I A+ +S+ S L G + Sbjct: 129 NLLPIWPLDGGRILLAVLCWFMSYRQATM----ISMTGSTLFAG--IMVGMSSLELKYNL 182 Query: 187 XMIGAFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAKPLDDILRL 246 +IG +LL N+ PY+F FL+ + Y+R Q G+A + I + + + Sbjct: 183 AVIGIYLLTLNIQAFLRFPYQFFRFLVEK---YDR-QLDGAAVQAIRVHPEETVLAVSHK 238 Query: 247 FKRNQYHFIYV-----MNEDKSIVAVVPEQK 272 +R H YV + E++ + A++ EQK Sbjct: 239 LRRGCSHLFYVQGAGLLAEEQLLYALLFEQK 269 >C2ZUW5.1 C2ZUW5_BACCE Stage IV sporulation protein FB Length = 274 Score = 139 bits (351), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 87/275 (31%), Positives = 137/275 (49%), Gaps = 16/275 (5%) Query: 11 LHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKSVQLLPFGGVAVM 70 +HPLF +I+++ + T +F ELL LF IV +HE+GH AA ++K +QLLPFGGVA M Sbjct: 1 MHPLFWVIIVIGIFTARFKELLLLFCIVLVHELGHAFAAAYYNWRIKQIQLLPFGGVAEM 60 Query: 71 EDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQANAIIALFNLLP 130 E+HG + E+ + +AGP+Q++ +I +AY L Q G+ T +F+ N II FNLLP Sbjct: 61 EEHGNKSLKEELIVVMAGPIQHIWMIAVAYILHQAGWVTDDLYHFFVWNNVIILGFNLLP 120 Query: 131 VLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXXXXXXXXXXXMIG 190 + PLDGGK+ + ++S +++G+ + + Sbjct: 121 IWPLDGGKVLFNVLSYRFPYLQAHEKMMKLSCVFFSVILGWQLLWNSNNIMMWVLLVFL- 179 Query: 191 AFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAKPLDDILRLFKRN 250 A LY +R Y F+ FL+ R Y ++ PI + L I F+R Sbjct: 180 AISLYQEWKQRR---YAFMRFLLER---YYGNKRDIEKIAPIEVQTEDRLYTIFTKFRRG 233 Query: 251 QYHFI---------YVMNEDKSIVAVVPEQKLISS 276 H I Y ++E++ + A E++ SS Sbjct: 234 YKHSIIVHGKYKEHYTLDENELLYAYFAEKRTTSS 268 >C2ZDD1.1 C2ZDD1_BACCE Stage IV sporulation protein FB Length = 274 Score = 139 bits (351), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 87/275 (31%), Positives = 137/275 (49%), Gaps = 16/275 (5%) Query: 11 LHPLFVLIMLLSVVTGQFLELLTLFTIVFIHEMGHVCAALLCGVQVKSVQLLPFGGVAVM 70 +HPLF +I+++ + T +F ELL LF IV +HE+GH AA ++K +QLLPFGGVA M Sbjct: 1 MHPLFWVIIVIGIFTARFKELLLLFCIVLVHELGHAFAAAYYNWRIKQIQLLPFGGVAEM 60 Query: 71 EDHGRLTAVREMGIAIAGPLQNVLLIVMAYGLRQTGYGTSAFLDYFIQANAIIALFNLLP 130 E+HG + E+ + +AGP+Q++ +I +AY L Q G+ T +F+ N II FNLLP Sbjct: 61 EEHGNKSLKEELIVVMAGPIQHIWMIAVAYILHQAGWVTDDLYHFFVWNNVIILGFNLLP 120 Query: 131 VLPLDGGKIFQAMXXXXXXXXXXXXWCGRVSIAASVLVVGYAMXXXXXXXXXXXXXXMIG 190 + PLDGGK+ + ++S +++G+ + + Sbjct: 121 IWPLDGGKVLFNVLSYRFPYLQAHEKMMKLSCVFFSVILGWQLLWNSNNIMMWVLLVFL- 179 Query: 191 AFLLYSNLTDQRNLPYRFVSFLMNREAVYERYQQSGSAARPIVAFSAKPLDDILRLFKRN 250 A LY +R Y F+ FL+ R Y ++ PI + L I F+R Sbjct: 180 AISLYQEWKQRR---YAFMRFLLER---YYGNKRDIEKIAPIEVQTEDRLYTIFTKFRRG 233 Query: 251 QYHFI---------YVMNEDKSIVAVVPEQKLISS 276 H I Y ++E++ + A E++ SS Sbjct: 234 YKHSIIVHGKYKEHYTLDENELLYAYFAEKRTTSS 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.144 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,322,054,594 Number of extensions: 45049484 Number of successful extensions: 135885 Number of sequences better than 1.0e-05: 566 Number of HSP's gapped: 135483 Number of HSP's successfully gapped: 566 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)