BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0011.p (390 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL38.1 B3KL38_9BACI Antifreeze protein type I 462 e-128 B1DC69.1 B1DC69_9BACL Antifreeze protein type I 441 e-122 A9KKC7.1 A9KKC7_CLOPH Antifreeze protein type I 403 e-110 C3WZM4.1 C3WZM4_9FUSO Antifreeze protein type I 334 8e-90 A1SUG8.1 A1SUG8_PSYIN Predicted antifreeze protein type I 279 4e-73 >B3KL38.1 B3KL38_9BACI Antifreeze protein type I Length = 435 Score = 462 bits (1188), Expect = e-128, Method: Compositional matrix adjust. Identities = 236/437 (54%), Positives = 286/437 (65%), Gaps = 53/437 (12%) Query: 1 MAIIEVVKYDGPPGVFAWKYPNQELGTWTQLIVNESQEAILFKGGQALDSFTAGRHTLST 60 MAIIEVVKY+GPP VFAW+YPNQELGTWTQLIV+E+QEAIL+KGGQALDSF AGRHTLST Sbjct: 1 MAIIEVVKYEGPPDVFAWRYPNQELGTWTQLIVHETQEAILYKGGQALDSFPAGRHTLST 60 Query: 61 ANIPLLSNLVNLPFGGKSPFTAEIWYVNKLNSMNVKWGTSAPLQLQDPKYKMMIAVRAFG 120 ANIP+LSN++NLPFGGKSPFTAE+W+VNKL S++VKWGTS+P+QLQDPKY +++++RAFG Sbjct: 61 ANIPILSNVINLPFGGKSPFTAEVWFVNKLRSLDVKWGTSSPIQLQDPKYNIIVSLRAFG 120 Query: 121 QFGVRIEEPRKFLLKLVGTLPQFDQSTMLSYFRGLLMSNINELISTYLVHKKISILEINA 180 QFGV+I +PRKFL +VGTLP FDQ T++ Y+RG+LMSNI E+IS+Y+V KKIS++EINA Sbjct: 121 QFGVQISDPRKFLGTMVGTLPTFDQGTLVKYYRGVLMSNITEIISSYIVRKKISVVEINA 180 Query: 181 YVSEISKHIQGRLASTFLESGIELNNFYXXXXXXXXXXXATVRLKEALAKKAEMDIIGFT 240 Y++EISKHI +A +F E GI L NFY A VR+KEALAKKAEMDIIG+T Sbjct: 181 YIAEISKHIMEAIAPSFEEMGITLLNFYVDSINIPEHDPAAVRIKEALAKKAEMDIIGYT 240 Query: 241 YQQERSFNTMEEAAGNPGSIGAGMVNTXXXXXXXXXFAGPASEMMNRMTRGIMEGGASAA 300 Y QER+FNT+E AA NPGS A M GP E M + Sbjct: 241 YHQERTFNTLEGAAKNPGSPAAVMGTGLGMGLGM---VGPMYETARHMFDHAKPQEKNDL 297 Query: 301 GSTIKTCPSCGAGNSAEARFCSGCGRSLQQPEPEGTPADRTACRNCGEQVVPG------- 353 K C CG N EARFCSGCG+S Q + E PA C +CG+ + P Sbjct: 298 QIEQKACTKCGMLNGEEARFCSGCGQSFQT-QAEEAPAKAILCNDCGQPLAPNAKFCLHC 356 Query: 354 ------------------------------------------AKFCPHCGTSVIIPCAGC 371 AKFCPHCG+S + C GC Sbjct: 357 GDPYHACPKCGHDHPAGAAECPDCGAPLPMPCRACGELVDAEAKFCPHCGSSHALTCPGC 416 Query: 372 GHVLQPGQKFCPECGTR 388 H ++PGQKFC ECG + Sbjct: 417 DHEIKPGQKFCMECGHK 433 >B1DC69.1 B1DC69_9BACL Antifreeze protein type I Length = 435 Score = 441 bits (1134), Expect = e-122, Method: Compositional matrix adjust. Identities = 221/432 (51%), Positives = 285/432 (65%), Gaps = 44/432 (10%) Query: 1 MAIIEVVKYDGPPGVFAWKYPNQELGTWTQLIVNESQEAILFKGGQALDSFTAGRHTLST 60 MA++EVVK+DGPP +F WKYPNQE+GTWTQLIVNE+QEA+LFKGG+ALD F GRHTLST Sbjct: 1 MAVVEVVKFDGPPDLFVWKYPNQEMGTWTQLIVNETQEAVLFKGGKALDLFGPGRHTLST 60 Query: 61 ANIPLLSNLVNLPFGGKSPFTAEIWYVNKLNSMNVKWGTSAPLQLQDPKYKMMIAVRAFG 120 ANIP + +LVNLPFGG+SPFTAE+WYVNK+NS++VKWGT++P+QLQDPKY+++++VR+FG Sbjct: 61 ANIPFIQSLVNLPFGGRSPFTAEVWYVNKINSLDVKWGTASPIQLQDPKYRILLSVRSFG 120 Query: 121 QFGVRIEEPRKFLLKLVGTLPQFDQSTMLSYFRGLLMSNINELISTYLVHKKISILEINA 180 QFGV+IE+ RKFL+KL+GTLP FD+ ++ YFR LLM NINELI+TYL KKISILEINA Sbjct: 121 QFGVQIEDSRKFLIKLIGTLPAFDKDALVKYFRSLLMMNINELITTYLAVKKISILEINA 180 Query: 181 YVSEISKHIQGRLASTFLESGIELNNFYXXXXXXXXXXXATVRLKEALAKKAEMDIIGFT 240 Y++EI+KHI+ R+ F + GI+L NFY T RLKEALAKKAEMDIIG+T Sbjct: 181 YITEIAKHIEERIGPVFQDYGIKLLNFYIDSINTPDDDPTTNRLKEALAKKAEMDIIGYT 240 Query: 241 YQQERSFNTMEEAAGNPGSIGAGMVNTXXXXXXXXXFAGPASEMMNRMTRGI-------- 292 YQQ+R+F+T+E AA N + +G++ G M+ +T+ + Sbjct: 241 YQQQRTFDTLEGAARNENTGSSGIMGAGIGLGMGAGIGGAFGGQMSGLTQQMNMTADKPC 300 Query: 293 -------MEG---------------GASAAGSTIKTCPSCGAGNSAEARFCSGCGRSLQQ 330 EG A A G C CG SA A+FC CG + Sbjct: 301 PQCHTTNREGSRFCNSCGYSFVSAPSAPAVGPKETECSVCGKSFSAAAKFCPHCGDKNRL 360 Query: 331 PEPEGTPADRTA--------------CRNCGEQVVPGAKFCPHCGTSVIIPCAGCGHVLQ 376 + GT A C +CG+ +V GAKFCP+CG S ++ C C H ++ Sbjct: 361 CQNCGTDNPDEAEFCVKCGHTVGIKLCSSCGDPLVAGAKFCPNCGASAVLKCGQCQHEVK 420 Query: 377 PGQKFCPECGTR 388 PGQKFC ECG + Sbjct: 421 PGQKFCLECGNK 432 >A9KKC7.1 A9KKC7_CLOPH Antifreeze protein type I Length = 433 Score = 403 bits (1035), Expect = e-110, Method: Compositional matrix adjust. Identities = 202/412 (49%), Positives = 259/412 (62%), Gaps = 32/412 (7%) Query: 1 MAIIEVVKYDGPPGVFAWKYPNQELGTWTQLIVNESQEAILFKGGQALDSFTAGRHTLST 60 MAI+EVVKYDG P VFAWKYPN ELGTWTQLIVNESQEA+LFKGG+ALD F GRHTL T Sbjct: 1 MAIVEVVKYDGAPNVFAWKYPNNELGTWTQLIVNESQEAVLFKGGKALDVFGGGRHTLET 60 Query: 61 ANIPLLSNLVNLPFGGKSPFTAEIWYVNKLNSMNVKWGTSAPLQLQDPKYKMMIAVRAFG 120 ANIPLL+N++NLPFG +SPF+AE+WYVNK+ S+N+KWGT++P+Q+QDPKY + I VR++G Sbjct: 61 ANIPLLNNIINLPFGRRSPFSAEVWYVNKVYSLNIKWGTTSPIQIQDPKYGIFIPVRSYG 120 Query: 121 QFGVRIEEPRKFLLKLVGTLPQFDQSTMLSYFRGLLMSNINELISTYLVHKKISILEINA 180 QFG+RIE+ +KFL+KLVGTL FD + +L YFRGL ++ + IS+YL+ KKIS+LEINA Sbjct: 121 QFGIRIEDSKKFLIKLVGTLNVFDDNNILQYFRGLYLTMAKDTISSYLIQKKISVLEINA 180 Query: 181 YVSEISKHIQGRLASTFLESGIELNNFYXXXXXXXXXXXATVRLKEALAKKAEMDIIGFT 240 Y+ E+S +I R+ T E GI L NFY A +LK+ALAK+AEM+IIG+ Sbjct: 181 YLDEMSNYIVERIKPTMDEYGIGLTNFYVNDINVPEEDTAVKKLKDALAKRAEMNIIGYD 240 Query: 241 YQQERSFNTMEEAAGNPGSIGAGMVNTXXXXXXXXXFAGPASEMMNRMTRGIMEGGASAA 300 YQQ+RSF+T+E AA N G + +G++ G + + Sbjct: 241 YQQQRSFDTLESAASNQGGVSSGIMGAGMGLGMGINVGNAIGTQF-----GSVSNALNTK 295 Query: 301 GSTIKTCPSCGAGNSAEARFCSGCGRSLQQPEPEGTPADRTACRNCGEQVVPGAKFCPHC 360 G K C C A A RFC CG + E E + + C NCG KFCP C Sbjct: 296 GGNKKECVKCHAAIEAGTRFCPSCGNDTSKTESENS--NTIKCDNCGAFYSANTKFCPEC 353 Query: 361 GT------------------------SVIIPCAGCGHVLQPGQ-KFCPECGT 387 G + IPC GCG+++ + KFCPECGT Sbjct: 354 GNRYNPCPNCKADIPDGATNCSSCGYELPIPCPGCGNLVHSKKHKFCPECGT 405 >C3WZM4.1 C3WZM4_9FUSO Antifreeze protein type I Length = 372 Score = 334 bits (857), Expect = 8e-90, Method: Compositional matrix adjust. Identities = 174/389 (44%), Positives = 241/389 (61%), Gaps = 20/389 (5%) Query: 1 MAIIEVVKYDGPPGVFAWKYPNQELGTWTQLIVNESQEAILFKGGQALDSFTAGRHTLST 60 MA++++VKY G F WKYP +LGTWTQL+VNE+QEA+L K G+ D FT G++ L + Sbjct: 1 MALVDIVKYSGTLEEFIWKYPRDDLGTWTQLVVNETQEALLLKDGKICDLFTTGKYILES 60 Query: 61 ANIPLLSNLVNLPFGGKSPFTAEIWYVNKLNSMNVKWGTSAPLQLQDPKYKMMIAVRAFG 120 NIP+L+ ++NLPFGG+SPF E+W++NK +++VKWGT +P+QLQD KYK+ I VR+FG Sbjct: 61 KNIPILNKIINLPFGGESPFKVEVWFINKRYNLDVKWGTPSPIQLQDAKYKIFIPVRSFG 120 Query: 121 QFGVRIEEPRKFLLKLVGTLPQFDQSTMLSYFRGLLMSNINELISTYLVHKKISILEINA 180 QFG+RIE+ + F K+VGT F ++ + +FRGL ++ I ++IS+YLV +KISILEINA Sbjct: 121 QFGIRIEDSKLFFSKIVGTKTIFTKNEINLFFRGLYLTKIKDMISSYLVKRKISILEINA 180 Query: 181 YVSEISKHIQGRLASTFLESGIELNNFYXXXXXXXXXXXATVRLKEALAKKAEMDIIGFT 240 Y+SE+S +I + F GIEL NFY RLKEALAK+AEMDI+G+ Sbjct: 181 YISELSDYISDSMKQVFKGYGIELVNFYINDINIPEDDSGVKRLKEALAKRAEMDIVGYN 240 Query: 241 YQQERSFNTMEEAAGNPGSIGAGMVNTXXXXXXXXXFAGPASEMMNRMTRGIMEGGASAA 300 Y QERSF+T+E AA N G+ + +N+ G + N++ ++ + Sbjct: 241 YLQERSFDTLEGAAKNAGT-SSNFINSGIGMAMG---VGLGNNFSNKVNSNLL---INTE 293 Query: 301 GSTIKTCPSCGAGNSAEARFCSGCGRSLQQPEPEGTPADRTACRNCGE-QVVPGAKFCPH 359 IK C CG A+FC CG + Q+ C NCG Q AKFC Sbjct: 294 EEKIK-CIKCGTELKRGAKFCFECGEAQQK-----------KCPNCGALQENQNAKFCSE 341 Query: 360 CGTSVIIPCAGCGHVLQPGQKFCPECGTR 388 CG ++ C CG V++ KFCPECG R Sbjct: 342 CGIPLVKKCKKCGKVVEENIKFCPECGNR 370 >A1SUG8.1 A1SUG8_PSYIN Predicted antifreeze protein type I Length = 351 Score = 279 bits (713), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 152/363 (41%), Positives = 216/363 (59%), Gaps = 17/363 (4%) Query: 1 MAIIEVVKYDGPPGVFAWKYPNQELGTWTQLIVNESQEAILFKGGQALDSFTAGRHTLST 60 M II+ VKY+GP VFAW++P+ L TQ+IVN++QEA+ +K G+ALD GRHTL + Sbjct: 1 MNIIDRVKYNGPGDVFAWRWPHDSLVWGTQVIVNQAQEAVFYKDGKALDVLGPGRHTLKS 60 Query: 61 ANIPLLSNLVNLPFGGKSPFTAEIWYVNKLNSMNVKWGTSAPLQLQDPKYKMMIAVRAFG 120 ANIPLL +LVN+ F +SPF AEI+YVNK ++N+KWGT P+ + +P Y + I +RAFG Sbjct: 61 ANIPLLEHLVNISFDNQSPFAAEIYYVNKAVNLNMKWGTQEPIPILEPVYNIYIPLRAFG 120 Query: 121 QFGVRIEEPRKFLLKLVGTLPQFDQSTMLSYFRGLLMSNINELISTYLVHKKISILEINA 180 QFG++I + +KF+ LVGT+ +FD +L+YFRG LMS I + IS +++ +IS+L I+A Sbjct: 121 QFGIKITDAKKFVTTLVGTVDEFDAEQILNYFRGHLMSRIKDFISKKVINDRISVLNISA 180 Query: 181 YVSEISKHIQGRLASTFLESGIELNNFYXXXXXXXXXXXATVRLKEALAKKAEMDIIGFT 240 +++E+S +Q + + F GIE+ NFY + +RLKE LAKKAE DI G T Sbjct: 181 HLNEMSAALQNDIMAEFKRFGIEIVNFYLNSINVPQNDDSVIRLKEILAKKAEFDIYGET 240 Query: 241 YQQERSFNTMEEAAGNPGSIGAGMVNTXXXXXXXXXFAGPASEMMNRMTRGIMEGGASA- 299 Y + F+ E+AA N G+ G G ++MM ++ ME S Sbjct: 241 YNTVKMFDVAEKAASNEGAGGVAGAGVGMGVG-----FGIGNQMM-KLNSTSMENEQSTI 294 Query: 300 -AGSTIKTCPSCGAGNSAEARFCSGCGRSLQQPEPEGTPADRTACRNCGEQVVPGAKFCP 358 AG + C SC A+FC CG Q + + C +CG + AKFCP Sbjct: 295 MAGPS---CSSCQQTIPQGAKFCPSCGSEQVQAQV------KKFCVDCGTERSLDAKFCP 345 Query: 359 HCG 361 CG Sbjct: 346 GCG 348 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,184,888,484 Number of extensions: 84542961 Number of successful extensions: 234654 Number of sequences better than 1.0e-05: 204 Number of HSP's gapped: 234527 Number of HSP's successfully gapped: 216 Length of query: 390 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 252 Effective length of database: 1,760,596,133 Effective search space: 443670225516 Effective search space used: 443670225516 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)