BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0008.p (145 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL42.1 B3KL42_9BACI Amino acid-binding ACT domain protein 225 1e-57 B1DDQ1.1 B1DDQ1_9BACL Amino acid-binding ACT domain protein 193 4e-48 C0ZAM1.1 C0ZAM1_BREBN ACT domain protein PheB 164 2e-39 A1HTV0.1 A1HTV0_9FIRM Amino acid-binding ACT domain protein 154 3e-36 Q5WHS7.1 Y1543_BACSK UPF0735 ACT domain-containing protein ABC1543 152 7e-36 >B3KL42.1 B3KL42_9BACI Amino acid-binding ACT domain protein Length = 201 Score = 225 bits (573), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 113/145 (77%), Positives = 127/145 (87%) Query: 1 VKERYYLVREDILPDAVLKTMQVKQLLEAGDAKTVHEGVEQVGLSRSAFYKYKDGIHLIH 60 VKERYYLVREDILP+AV+KTMQVK+LL +GD +TVHE VEQVGLSRSAFYKYKDGIH I+ Sbjct: 57 VKERYYLVREDILPEAVVKTMQVKKLLASGDVRTVHEAVEQVGLSRSAFYKYKDGIHPIN 116 Query: 61 QLERERIVTISIDLEHESGMLSKVLGSVAVHGANVLTIHQSIPLQGRANVVISVEISHLN 120 QLER+ IVTISID+EH SGMLS+VLG VA G NVLTIHQSIPLQG ANVVISVE+S LN Sbjct: 117 QLERDEIVTISIDMEHRSGMLSEVLGLVAGEGGNVLTIHQSIPLQGIANVVISVEVSRLN 176 Query: 121 EELGDMLDSLKAIPGVKRALIIGQG 145 EEL D+LD L+ I GV+R +IGQG Sbjct: 177 EELDDLLDGLRRIAGVRRVQMIGQG 201 >B1DDQ1.1 B1DDQ1_9BACL Amino acid-binding ACT domain protein Length = 145 Score = 193 bits (490), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 92/143 (64%), Positives = 122/143 (85%) Query: 3 ERYYLVREDILPDAVLKTMQVKQLLEAGDAKTVHEGVEQVGLSRSAFYKYKDGIHLIHQL 62 +RYY+VRED+LP+A++KT+QVK++L G+A TVHE VE+VGLSRSAFYKYKDG++ + +L Sbjct: 3 QRYYVVREDMLPEAIVKTIQVKEMLGRGEASTVHEAVEKVGLSRSAFYKYKDGVYALQEL 62 Query: 63 ERERIVTISIDLEHESGMLSKVLGSVAVHGANVLTIHQSIPLQGRANVVISVEISHLNEE 122 ERERIVTIS+DLEH SG+LSKVL VA NVLT++QSIPLQG ANVV+SV+IS LNEE Sbjct: 63 ERERIVTISMDLEHRSGVLSKVLALVASLEGNVLTMNQSIPLQGLANVVLSVDISQLNEE 122 Query: 123 LGDMLDSLKAIPGVKRALIIGQG 145 L +++ +++ GV++A +IGQG Sbjct: 123 LTTLIEVIRSQEGVRKAAVIGQG 145 >C0ZAM1.1 C0ZAM1_BREBN ACT domain protein PheB Length = 147 Score = 164 bits (415), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 75/144 (52%), Positives = 111/144 (77%) Query: 2 KERYYLVREDILPDAVLKTMQVKQLLEAGDAKTVHEGVEQVGLSRSAFYKYKDGIHLIHQ 61 +E++YL+R DILP+++ KT++ K++LE+G+ TV+E VE+VGLSRSAFYKYKDGI + Sbjct: 4 EEKFYLIRSDILPESIAKTIEAKKMLESGEVDTVNEAVERVGLSRSAFYKYKDGIFPFNA 63 Query: 62 LERERIVTISIDLEHESGMLSKVLGSVAVHGANVLTIHQSIPLQGRANVVISVEISHLNE 121 + E+I+T + L+H SG LSKVL VA G NVLTI+Q+IPLQG A + +SV++++L Sbjct: 64 MMSEKIMTFTFSLDHMSGYLSKVLTYVADQGGNVLTINQTIPLQGIATISMSVDMANLRV 123 Query: 122 ELGDMLDSLKAIPGVKRALIIGQG 145 + LD L+ IPGV++A+I+G+G Sbjct: 124 SSTEFLDGLQLIPGVRKAMIVGRG 147 >A1HTV0.1 A1HTV0_9FIRM Amino acid-binding ACT domain protein Length = 149 Score = 154 bits (389), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 74/144 (51%), Positives = 110/144 (76%) Query: 2 KERYYLVREDILPDAVLKTMQVKQLLEAGDAKTVHEGVEQVGLSRSAFYKYKDGIHLIHQ 61 K +YLVRE+ILP+A+ KT++VK+LL+ G+AKT++E VE++GLSRSA+YKYKD + ++ Sbjct: 6 KSAFYLVREEILPEAIKKTIKVKELLKRGEAKTINEAVEKMGLSRSAYYKYKDFVFPFYE 65 Query: 62 LERERIVTISIDLEHESGMLSKVLGSVAVHGANVLTIHQSIPLQGRANVVISVEISHLNE 121 +E+IVTI++ LEH+ G+LS+VL ++A +VLTI+Q IPLQG AN IS+E + L Sbjct: 66 ASKEKIVTIALLLEHKPGVLSRVLNTIAGDRGSVLTINQGIPLQGVANATISIETADLAV 125 Query: 122 ELGDMLDSLKAIPGVKRALIIGQG 145 +L +LD L+ + GVKR ++GQ Sbjct: 126 DLEALLDKLRMVEGVKRLELLGQA 149 >Q5WHS7.1 Y1543_BACSK UPF0735 ACT domain-containing protein ABC1543 Length = 147 Score = 152 bits (385), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 70/144 (48%), Positives = 107/144 (74%) Query: 2 KERYYLVREDILPDAVLKTMQVKQLLEAGDAKTVHEGVEQVGLSRSAFYKYKDGIHLIHQ 61 K+++YLVRED+L DA+ +T++ K LL AG + ++E V +VGLSRSAFYKYKDGI H Sbjct: 3 KKQFYLVREDMLTDAMQRTLEAKALLSAGKVRKINEAVHRVGLSRSAFYKYKDGIFPFHA 62 Query: 62 LERERIVTISIDLEHESGMLSKVLGSVAVHGANVLTIHQSIPLQGRANVVISVEISHLNE 121 + +ERI+T SI+L SG LS++L VA GAN+LTI+Q+IPLQGRAN+ +SV+ + + Sbjct: 63 IAKERIMTFSINLADRSGTLSQLLNIVADTGANILTINQTIPLQGRANITLSVDTAPIEI 122 Query: 122 ELGDMLDSLKAIPGVKRALIIGQG 145 ++ ++ + ++A+ V+R ++G G Sbjct: 123 DINELFERMEALESVERVELVGSG 146 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 797,081,719 Number of extensions: 28891822 Number of successful extensions: 82625 Number of sequences better than 1.0e-05: 146 Number of HSP's gapped: 82586 Number of HSP's successfully gapped: 146 Length of query: 145 Length of database: 3,060,696,203 Length adjustment: 108 Effective length of query: 37 Effective length of database: 2,043,226,583 Effective search space: 75599383571 Effective search space used: 75599383571 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)