BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0005.p (324 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL45.1 B3KL45_9BACI Homoserine kinase EC=2.7.1.39 423 e-116 B1DDP9.1 B1DDP9_9BACL Homoserine kinase 380 e-103 C0ZAM4.1 C0ZAM4_BREBN Homoserine kinase EC=2.7.1.39 297 1e-78 Q1IW37.1 Q1IW37_DEIGD Homoserine kinase 254 8e-66 C1CYA9.1 C1CYA9_DEIDV Putative Homoserine kinase 234 1e-59 >B3KL45.1 B3KL45_9BACI Homoserine kinase EC=2.7.1.39 Length = 322 Score = 423 bits (1087), Expect = e-116, Method: Compositional matrix adjust. Identities = 210/316 (66%), Positives = 247/316 (78%), Gaps = 4/316 (1%) Query: 6 RVKVPASTANLGPGFDTLGMALSLYAWIEMEESAETEFHLYGDEMAGVAKDKSNLLYQVA 65 RVKVPASTANLGPGFDTLGMALSLY+WIEM+ S +T FHL+GDEM G+ DKSNL+Y+VA Sbjct: 8 RVKVPASTANLGPGFDTLGMALSLYSWIEMKASEKTVFHLHGDEMGGLPTDKSNLIYKVA 67 Query: 66 QMVFSEAGVAVPELSITMYSDIPLTRGLGSSASAIVGGMAAANAMIGSPLSNAKLFDMAT 125 QMVF E G AVP+L I+MYS+IPLTRGLGSSASAIVG +AAANA+IGSPL ++KLFDMAT Sbjct: 68 QMVFEEVGQAVPDLEISMYSEIPLTRGLGSSASAIVGALAAANALIGSPLPDSKLFDMAT 127 Query: 126 ALEKHPDNVGASLFGGIITAVWDGEHADYIRIEPPEALEILVVIPDFELSTTKARGVLPS 185 LEKHPDNVGASLFGGII AVWDG HADY+RIEPP L LVVIPDFEL+T+KAR LP+ Sbjct: 128 NLEKHPDNVGASLFGGIIAAVWDGSHADYMRIEPPADLGTLVVIPDFELATSKAREALPA 187 Query: 186 QITVGDAVHNIXXXXXXXXXXXXXXXDLIGRAMQDRLHQPYRAPLVPGMEKLLAEATGHG 245 Q+++ DAV+NI DLI AM+DR+HQPYRAPLVPGM K+LAEA HG Sbjct: 188 QVSLADAVYNISRSSLMTAALSAGRLDLIRAAMKDRIHQPYRAPLVPGMSKILAEAPDHG 247 Query: 246 ALGIALSGAGPTLLCMVDRQESRKPELELFLKQTMEEHGIPARTCWLPPSSSGVAVEQIE 305 ALGIALSGAGPT++ +VDR E R EL +L +TM+ GI + L P + GV + Sbjct: 248 ALGIALSGAGPTMIALVDRNEPRAEELGTYLTETMKAEGILSSCYPLNPVAEGVQLL--- 304 Query: 306 PNQMQNRSFLDMIKGE 321 + + NRSF DMI+GE Sbjct: 305 -DDINNRSFSDMIRGE 319 >B1DDP9.1 B1DDP9_9BACL Homoserine kinase Length = 324 Score = 380 bits (975), Expect = e-103, Method: Compositional matrix adjust. Identities = 190/318 (59%), Positives = 224/318 (70%), Gaps = 1/318 (0%) Query: 4 RSRVKVPASTANLGPGFDTLGMALSLYAWIEMEESAETEFHLYGDEMAGVAKDKSNLLYQ 63 R VKVPASTANLGPGFDTLGMALSLYAWIEM + T F LYGD+M G+ DKSNLLY+ Sbjct: 5 RVIVKVPASTANLGPGFDTLGMALSLYAWIEMSAAETTTFRLYGDQMQGIPTDKSNLLYK 64 Query: 64 VAQMVFSEAGVAVPELSITMYSDIPLTRGLGSSASAIVGGMAAANAMIGSPLSNAKLFDM 123 VAQ+VF EAGV+VPEL + MYSDIPL RGLGSSASAIVG + AANA+IGSPL++ KLF + Sbjct: 65 VAQLVFEEAGVSVPELDVAMYSDIPLARGLGSSASAIVGALVAANALIGSPLTDHKLFQL 124 Query: 124 ATALEKHPDNVGASLFGGIITAVWDGEHADYIRIEPPEALEILVVIPDFELSTTKARGVL 183 AT LE HPDNVGASLFGGI+ + WDGE ADY+R+ P LE LV IP F+L+T KAR L Sbjct: 125 ATKLEGHPDNVGASLFGGIVVSAWDGERADYVRLAPHAKLETLVAIPAFQLATEKARHAL 184 Query: 184 PSQITVGDAVHNIXXXXXXXXXXXXXXXDLIGRAMQDRLHQPYRAPLVPGMEKLLAEATG 243 P QI++ DAV N+ LI AM+DRLHQPYRAPL+PGM +L +A Sbjct: 185 PKQISMADAVFNVGRSSLLVAALASGELGLIRHAMRDRLHQPYRAPLIPGMSTILEQAVE 244 Query: 244 HGALGIALSGAGPTLLCMVDRQESRKPELELFLKQTMEEHGIPARTCWLPPSSSGVAVEQ 303 +GALG ALSGAGPTL+ VD K ELE FL T+++ GI A T WL P G + Sbjct: 245 YGALGAALSGAGPTLIAFVDADSKSKSELEQFLLATLKQEGIEAETMWLKPGGEGPLI-T 303 Query: 304 IEPNQMQNRSFLDMIKGE 321 +E ++ IKGE Sbjct: 304 VEDAATPTPGLMERIKGE 321 >C0ZAM4.1 C0ZAM4_BREBN Homoserine kinase EC=2.7.1.39 Length = 309 Score = 297 bits (760), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 155/302 (51%), Positives = 196/302 (64%), Gaps = 4/302 (1%) Query: 4 RSRVKVPASTANLGPGFDTLGMALSLYAWIEMEESAETEFHLYGDEMAGVAKDKSNLLYQ 63 R +V +PA+TANLGPGFD LGMA LY+ +EME S T + G E+ G DK+NLLY+ Sbjct: 5 RVKVTIPATTANLGPGFDALGMAFQLYSVVEMEISDHTTVEMMGKELQGTPTDKNNLLYK 64 Query: 64 VAQMVFSEAGVAVPELSITMYSDIPLTRGLGSSASAIVGGMAAANAMIGSPLSNAKLFDM 123 VA +F EAG+A+PEL+I S+ PLTRGLGSSA+AIVG + AAN + G P + +LF+ Sbjct: 65 VAAGLFQEAGLAIPELAIRASSEAPLTRGLGSSAAAIVGALVAANHLAGEPFTREQLFEK 124 Query: 124 ATALEKHPDNVGASLFGGIITAVW---DGEHADYIRIEPPEALEILVVIPDFELSTTKAR 180 AT LE HPDN GAS+FGGII A G+ Y+R P L+ LVVIP+F LST KAR Sbjct: 125 ATRLEGHPDNAGASMFGGIIVATMPEVPGDPVPYVRFSAPAGLQTLVVIPEFMLSTEKAR 184 Query: 181 GVLPSQITVGDAVHNIXXXXXXXXXXXXXXXDLIGRAMQDRLHQPYRAPLVPGMEKLLAE 240 VLP + D V+N+ DL+GRAM DRLHQPYRA LVPG+ ++L + Sbjct: 185 NVLPQVYSREDVVYNVGHSSLLVAALAQGRLDLMGRAMSDRLHQPYRAKLVPGLNEILDK 244 Query: 241 ATGHGALGIALSGAGPTLLCMVDRQESRKPELELFLKQTMEEHGIPARTCWLPPSSSGVA 300 AT +GALG ALSGAGPT+LC E + +L F+ + M+ H I R L P +GV Sbjct: 245 ATENGALGAALSGAGPTILCFFSSDEELE-KLRAFVDRVMKGHDISYRVMILQPDENGVQ 303 Query: 301 VE 302 VE Sbjct: 304 VE 305 >Q1IW37.1 Q1IW37_DEIGD Homoserine kinase Length = 317 Score = 254 bits (649), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 135/308 (43%), Positives = 190/308 (61%), Gaps = 3/308 (0%) Query: 7 VKVPASTANLGPGFDTLGMALSLYAWIEMEESAETEFHLYGDEMAGVAKDKSNLLYQVAQ 66 V+ PAS+ANLGPGFD+LG+++ L+ + + TE G +AG DKSN +Y+ Q Sbjct: 5 VRAPASSANLGPGFDSLGLSVPLFTTLRVTPQEVTEVVPLGPALAGTPADKSNYVYRAMQ 64 Query: 67 MVFSEAGVAVPELSITMYSDIPLTRGLGSSASAIVGGMAAANAMIGSPLSNAKLFDMATA 126 + AG +P + + +++PL RGLGSSA+A+V G+ AAN ++G PL + L D+A Sbjct: 65 LAAKRAGRPLPPARVEIETEVPLARGLGSSAAALVAGIVAANELLGRPLDDEALLDVAAR 124 Query: 127 LEKHPDNVGASLFGGIITAVWDGEHADYIRIEPPEALEILVVIPDFELSTTKARGVLPSQ 186 E HPDNV +LFGGI+ A D Y+R+EPP L + V+IPDFELST+KAR VLP + Sbjct: 125 EEGHPDNVAPALFGGIVVATLDKLGTHYVRLEPPAHLGVTVLIPDFELSTSKARAVLPKE 184 Query: 187 ITVGDAVHNIXXXXXXXXXXXXXXXDLIGRAMQDRLHQPYRAPLVPGMEKLLAEATGHGA 246 + DAVH + DL+ AMQD +HQ +RAPLVPG+ +L EA HGA Sbjct: 185 YSRADAVHALSHAALLAAALAQGRLDLLKHAMQDYIHQIWRAPLVPGLSDILEEAHRHGA 244 Query: 247 LGIALSGAGPTLLCMVDRQESRKPELELFLKQTMEEHGIPARTCWLPPSSSGVAVE--QI 304 LG ALSGAGPT+LC D ++ + +L +L+ + ++G+ R LP ++G VE Q Sbjct: 245 LGAALSGAGPTVLCFHDTRD-KTDDLHRYLQGVLNKNGLTGRVLDLPIDTAGTVVEAAQC 303 Query: 305 EPNQMQNR 312 P+ Q R Sbjct: 304 LPSADQGR 311 >C1CYA9.1 C1CYA9_DEIDV Putative Homoserine kinase Length = 315 Score = 234 bits (596), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 122/282 (43%), Positives = 172/282 (60%), Gaps = 1/282 (0%) Query: 7 VKVPASTANLGPGFDTLGMALSLYAWIEMEESAETEFHLYGDEMAGVAKDKSNLLYQVAQ 66 V+ PAS+ANLGPGFD+LG+++ LY + + TE G E+ D+SN +YQ Sbjct: 20 VRAPASSANLGPGFDSLGLSVPLYTTLRVTPQEVTEVVPCGPELENTPADESNYVYQAML 79 Query: 67 MVFSEAGVAVPELSITMYSDIPLTRGLGSSASAIVGGMAAANAMIGSPLSNAKLFDMATA 126 + AG +P I + +++PL RGLGSSA+A++ G+ A N ++G PL + + D+A Sbjct: 80 LSAQRAGRTLPPARIEIETEVPLARGLGSSAAALIAGIVAGNELLGRPLDHETVLDVAAR 139 Query: 127 LEKHPDNVGASLFGGIITAVWDGEHADYIRIEPPEALEILVVIPDFELSTTKARGVLPSQ 186 E HPDNV +LFGGI+ A D Y+R++PP L + V+IPDFELST+KAR VLP + Sbjct: 140 EEGHPDNVAPALFGGIVIATLDKLGTHYVRLDPPAHLGVTVLIPDFELSTSKARAVLPRE 199 Query: 187 ITVGDAVHNIXXXXXXXXXXXXXXXDLIGRAMQDRLHQPYRAPLVPGMEKLLAEATGHGA 246 + DAVH + DL+ AMQD +HQ +RAPLVPG+ +L +A +GA Sbjct: 200 YSRADAVHALSHAALLAAALSVGRLDLLRHAMQDYIHQVWRAPLVPGLSDILDDAHRYGA 259 Query: 247 LGIALSGAGPTLLCMVDRQESRKPELELFLKQTMEEHGIPAR 288 LG ALSGAGPT+LC D +E L +L M+ +G+ R Sbjct: 260 LGAALSGAGPTVLCFHDTREPTS-RLHTYLHSVMKRNGLSGR 300 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,815,805,194 Number of extensions: 71050555 Number of successful extensions: 235821 Number of sequences better than 1.0e-05: 1083 Number of HSP's gapped: 233386 Number of HSP's successfully gapped: 1133 Length of query: 324 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 188 Effective length of database: 1,779,438,163 Effective search space: 334534374644 Effective search space used: 334534374644 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)