BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0015.p (473 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ61.1 B3KJ61_9BACI Nitrate reductase, alpha subunit EC=1.7.99.4 845 0.0 Q5WK51.1 Q5WK51_BACSK Respiratory nitrate reductase alpha chain ... 823 0.0 B9E9D2.1 B9E9D2_MACCJ Respiratory nitrate reductase alpha chain 785 0.0 C2LWN3.1 C2LWN3_STAHO Nitrate reductase, alpha subunit EC=1.7.99.4 777 0.0 Q6GE36.1 Q6GE36_STAAR Nitrate reductase alpha chain EC=1.7.99.4 775 0.0 >B3KJ61.1 B3KJ61_9BACI Nitrate reductase, alpha subunit EC=1.7.99.4 Length = 1224 Score = 845 bits (2182), Expect = 0.0, Method: Compositional matrix adjust. Identities = 397/473 (83%), Positives = 433/473 (91%), Gaps = 2/473 (0%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +VALDFRMT+TP+YAD+VLPAATWYEKTDLSSTDMHPFVHPFNPAV+PLWESRSDWDIYR Sbjct: 754 LVALDFRMTSTPMYADVVLPAATWYEKTDLSSTDMHPFVHPFNPAVDPLWESRSDWDIYR 813 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 +AEVFS+MAK+ LPGVYKDLV SPLAHDS SEI+QP G VKDW KGEVEAVPGKTMPNL Sbjct: 814 TIAEVFSDMAKTHLPGVYKDLVTSPLAHDSASEIAQPYGEVKDWRKGEVEAVPGKTMPNL 873 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 ++VERDYTKI+DKY++LGPNL GK GAHG+SFSVAEEYEELKRLNG +YD+SIKNGLPK Sbjct: 874 TVVERDYTKIYDKYVTLGPNLATGKVGAHGVSFSVAEEYEELKRLNGTYYDDSIKNGLPK 933 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 + TAR AD IL+LSSATNGRV+Q+A+E AE+D+GV L+DISADRAAE+ITFQSITAQPR Sbjct: 934 LHTARHAADAILNLSSATNGRVAQRAWESAEQDTGVQLKDISADRAAERITFQSITAQPR 993 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 EVIPTPVF K RRYSPFTTNIERLVPFRTLTGRQHFYIDHEIF QFGEALPVYKPT Sbjct: 994 EVIPTPVFSGSNKMGRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFLQFGEALPVYKPT 1053 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 LPPMVFGPRD+++ GG+D LVLRYLTPHGKWNIHSTYQDN HMLTLFRGGPT INN+ Sbjct: 1054 LPPMVFGPRDKQITGGKDTLVLRYLTPHGKWNIHSTYQDNLHMLTLFRGGPT--VWINND 1111 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 DA + I DNDWLEVYNRNGVVTARAVVSHRMP+GTM+MYHAQDKHI VPGSEIT RGG Sbjct: 1112 DAAANDIQDNDWLEVYNRNGVVTARAVVSHRMPKGTMYMYHAQDKHINVPGSEITKDRGG 1171 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 SHNAPTRIH+KPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVR KEV+WLED Sbjct: 1172 SHNAPTRIHVKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRKMKEVDWLED 1224 >Q5WK51.1 Q5WK51_BACSK Respiratory nitrate reductase alpha chain EC=1.7.99.4 Length = 1224 Score = 823 bits (2126), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/473 (82%), Positives = 425/473 (89%), Gaps = 2/473 (0%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +VALDFRMT TP+YADIVLPAATWYEKTDLSSTDMHPFVHPFNPAV+PLWESRSDWDIYR Sbjct: 754 LVALDFRMTATPIYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVDPLWESRSDWDIYR 813 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 LA+ FSEMAK LPGV+KDLVA+PLAHDS SEI+QP G+VKDW KGE+EAVPGKTMPNL Sbjct: 814 TLAKTFSEMAKDHLPGVHKDLVATPLAHDSASEIAQPYGVVKDWRKGEIEAVPGKTMPNL 873 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 +IVERDYT+I++KYISLGP L GK GAHG+SF VAEEYEELKR+NG++ D++IK GLPK Sbjct: 874 TIVERDYTQIYNKYISLGPGLANGKVGAHGVSFPVAEEYEELKRINGIYEDDTIKQGLPK 933 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 + TAR A+TIL LSSATNGRVSQ+A+E E+D+GV L DISADR+AE+ITFQSITAQPR Sbjct: 934 LYTARHAAETILTLSSATNGRVSQRAWEALEQDTGVQLADISADRSAERITFQSITAQPR 993 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 EVIPTPVF K RRYSPFTTNIERLVPFRTLTGRQHFYIDHEIF QFGEALPVYKPT Sbjct: 994 EVIPTPVFTGSNKMGRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFLQFGEALPVYKPT 1053 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 LPPMVFGPRDR+V G + LVLRYLTPHGKWNIHSTYQDN HMLTLFRGGPT +NNE Sbjct: 1054 LPPMVFGPRDRQVDGSDETLVLRYLTPHGKWNIHSTYQDNLHMLTLFRGGPT--VWLNNE 1111 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 DA H I DNDWLEVYNRNGVVTARAVVSHRMP+GTMFMYHAQDKHI VPGSEIT RGG Sbjct: 1112 DAAAHHIQDNDWLEVYNRNGVVTARAVVSHRMPKGTMFMYHAQDKHIYVPGSEITKNRGG 1171 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 SHNAPTRIH+KPTQ+VGGYAQLSYGFNYYGPIGNQRDVYVAVR KEV+WLED Sbjct: 1172 SHNAPTRIHVKPTQVVGGYAQLSYGFNYYGPIGNQRDVYVAVRKMKEVDWLED 1224 >B9E9D2.1 B9E9D2_MACCJ Respiratory nitrate reductase alpha chain Length = 1224 Score = 785 bits (2026), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/473 (77%), Positives = 416/473 (87%), Gaps = 2/473 (0%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +VALDFRMTTTPLYAD+VLPAATWYEK D+SSTDMHPFVHPFNPAV+PLWESRSDWDIY+ Sbjct: 754 LVALDFRMTTTPLYADVVLPAATWYEKHDISSTDMHPFVHPFNPAVDPLWESRSDWDIYK 813 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 +A+ FSEM++ L G YKD+V +PLAHDS EIS PMG+V+DW KGEVE +PGKTMP Sbjct: 814 SIAKTFSEMSERHLKGTYKDVVTAPLAHDSQQEISTPMGIVRDWTKGEVEPIPGKTMPGF 873 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 ++V+R YT I+DKY+S+GP L GK GAHG+SFSV EEY EL+ + G + D+++KNGLP+ Sbjct: 874 AVVDRTYTDIYDKYVSVGPLLENGKVGAHGVSFSVKEEYNELRSMVGTYEDDTVKNGLPR 933 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 I TA++VAD IL++SSATNG VSQK+YE E +G+ L+DISA+R AEKITFQ+ITAQPR Sbjct: 934 IDTAKRVADVILNVSSATNGHVSQKSYEDLENQTGMELKDISAERTAEKITFQNITAQPR 993 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 EVIPT VF K RRYSPFTTNIERLVPFRTLTGRQ +YIDHE+FQQFGE+LPVYKPT Sbjct: 994 EVIPTAVFPGSNKLGRRYSPFTTNIERLVPFRTLTGRQSYYIDHEVFQQFGESLPVYKPT 1053 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 LPPMVFG +D+ +KGG D+LVLRYLTPHGKWNIHSTYQDN HMLTLFRGGPT INNE Sbjct: 1054 LPPMVFGTKDKTIKGGVDSLVLRYLTPHGKWNIHSTYQDNLHMLTLFRGGPT--VWINNE 1111 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 DA QH I+DNDWLEVYNRNG+VTARAVVSHRMPRGTMFMYHAQDKHIQ PGSEITDTRGG Sbjct: 1112 DAAQHNINDNDWLEVYNRNGLVTARAVVSHRMPRGTMFMYHAQDKHIQTPGSEITDTRGG 1171 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 SHNAPTRIHLKPTQ+VGGYAQ+SYGFNYYGPIGNQRDVYVAVR KEV+WLED Sbjct: 1172 SHNAPTRIHLKPTQLVGGYAQISYGFNYYGPIGNQRDVYVAVRKMKEVDWLED 1224 >C2LWN3.1 C2LWN3_STAHO Nitrate reductase, alpha subunit EC=1.7.99.4 Length = 1227 Score = 777 bits (2006), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/476 (76%), Positives = 416/476 (87%), Gaps = 5/476 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +V+LDFRMT TPLY+DIVLPAATWYEK DLSSTDMHPF+HPFNPA++PLWESRSDWDIY+ Sbjct: 754 LVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPFIHPFNPAIDPLWESRSDWDIYK 813 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 L++ SEMAK LPG +KD+V +PLAHDS E+ G+VKDW+KGEVE +PGKTMPN Sbjct: 814 TLSKAISEMAKDYLPGTFKDVVTTPLAHDSKQELGSEYGIVKDWSKGEVEGMPGKTMPNF 873 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDE---SIKNG 177 SIVERDYTKI+DKY++LGP L GK GAHG+SFSV EEYEELK + G + D+ S++N Sbjct: 874 SIVERDYTKIYDKYVTLGPLLEKGKVGAHGVSFSVKEEYEELKSMIGTWNDDDEHSVRND 933 Query: 178 LPKIQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITA 237 P+I TAR+VAD IL++SSATNG++SQK+YE E +G+ L+DIS +RA+EKI+F +ITA Sbjct: 934 RPRIDTARKVADAILNISSATNGKLSQKSYEDLENQTGMELKDISKERASEKISFLNITA 993 Query: 238 QPREVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVY 297 QPREVIPT VF K RRYSPFTTNIERLVPFRTLTGRQ +YIDHE+FQQFGE+LPVY Sbjct: 994 QPREVIPTAVFPGSNKNNRRYSPFTTNIERLVPFRTLTGRQSYYIDHEVFQQFGESLPVY 1053 Query: 298 KPTLPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXI 357 KPTLPPMVFG RD++VKGGQDALVLRYLTPHGKWNIHSTYQDN+ MLTLFRGGP + Sbjct: 1054 KPTLPPMVFGARDKKVKGGQDALVLRYLTPHGKWNIHSTYQDNERMLTLFRGGPV--VWL 1111 Query: 358 NNEDAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDT 417 +NEDAEQHGI+DNDWLEVYNRNGVVTARAV SHRMP+GTMFMYHAQDKHI+ PGSEITDT Sbjct: 1112 SNEDAEQHGINDNDWLEVYNRNGVVTARAVTSHRMPKGTMFMYHAQDKHIETPGSEITDT 1171 Query: 418 RGGSHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 RGGSHNAPTRIHLKPTQ+VGGYAQ+SY FNYYGPIGNQRDVYVAVR KEVNWLED Sbjct: 1172 RGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDVYVAVRKMKEVNWLED 1227 >Q6GE36.1 Q6GE36_STAAR Nitrate reductase alpha chain EC=1.7.99.4 Length = 1229 Score = 775 bits (2001), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/476 (75%), Positives = 420/476 (88%), Gaps = 5/476 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +V+LDFRMT TPLY+DIVLPAATWYEK DLSSTDMHP+VHPFNPA++PLWESRSDWDIY+ Sbjct: 756 VVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFNPAIDPLWESRSDWDIYK 815 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 LA+ FSEMAK LPG +KD+V +PL+HD+ EIS P G+VKDW+KGE+EAVPG+TMPN Sbjct: 816 TLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVVKDWSKGEIEAVPGRTMPNF 875 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYD---ESIKNG 177 +IVERDYTKI+DKY++LGP L GK GAHG+SF V+E+YEELK + G + D +S++ Sbjct: 876 AIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQYEELKSMLGTWSDTNDDSVRAN 935 Query: 178 LPKIQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITA 237 P+I TAR VAD IL++SSATNG++SQK+YE E+ +G+ L+DIS++RAAEKI+F +IT+ Sbjct: 936 RPRIDTARNVADAILNISSATNGKLSQKSYEDLEEQTGMPLKDISSERAAEKISFLNITS 995 Query: 238 QPREVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVY 297 QPREVIPT VF K+ RRYSPFTTN+ERLVPFRTLTGRQ +Y+DHE+FQQFGE+LPVY Sbjct: 996 QPREVIPTAVFPGSNKQGRRYSPFTTNVERLVPFRTLTGRQSYYVDHEVFQQFGESLPVY 1055 Query: 298 KPTLPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXI 357 KPTLPPMVFG RD+++KGG DALVLRYLTPHGKWNIHS YQDN+HMLTLFRGGPT I Sbjct: 1056 KPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGKWNIHSMYQDNKHMLTLFRGGPT--VWI 1113 Query: 358 NNEDAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDT 417 +NEDAE+H I DNDWLEVYNRNGVVTARAV+SHRMP+GTMFMYHAQDKHIQ PGSEITDT Sbjct: 1114 SNEDAEKHDIQDNDWLEVYNRNGVVTARAVISHRMPKGTMFMYHAQDKHIQTPGSEITDT 1173 Query: 418 RGGSHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 RGGSHNAPTRIHLKPTQ+VGGYAQ+SY FNYYGPIGNQRD+YVAVR KEVNWLED Sbjct: 1174 RGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDLYVAVRKMKEVNWLED 1229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,968,731,765 Number of extensions: 127906311 Number of successful extensions: 266337 Number of sequences better than 1.0e-05: 590 Number of HSP's gapped: 265344 Number of HSP's successfully gapped: 609 Length of query: 473 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 333 Effective length of database: 1,741,754,103 Effective search space: 580004116299 Effective search space used: 580004116299 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)