BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0014.p (517 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ62.1 B3KJ62_9BACI Nitrate reductase, beta subunit 903 0.0 B9E9D3.1 B9E9D3_MACCJ Respiratory nitrate reductase beta chain 899 0.0 Q5WK50.1 Q5WK50_BACSK Respiratory nitrate reductase beta chain E... 850 0.0 C2G8E2.1 C2G8E2_STAAU Nitrate reductase beta chain EC=1.7.99.4 836 0.0 Q6GE37.1 Q6GE37_STAAR Nitrate reductase beta chain EC=1.7.99.4 836 0.0 >B3KJ62.1 B3KJ62_9BACI Nitrate reductase, beta subunit Length = 511 Score = 903 bits (2334), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/517 (81%), Positives = 463/517 (89%), Gaps = 14/517 (2%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGWQL 60 MVMNLDKCIGCHTCSVTCK+TWTNR GAEYMWFNNVETKPG+GYP RWEDQE YKGGW L Sbjct: 9 MVMNLDKCIGCHTCSVTCKSTWTNRPGAEYMWFNNVETKPGVGYPIRWEDQEKYKGGWTL 68 Query: 61 RKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAHS 120 +KGKL+LKSG+KLSK+ALGKIFYNPDMPEMKDYYEPWTYNYEHLT AGE+KH PVAR S Sbjct: 69 KKGKLQLKSGSKLSKIALGKIFYNPDMPEMKDYYEPWTYNYEHLTNAGEKKHQPVARPKS 128 Query: 121 AVTGEKMDLEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHCLN 180 AVTGE M+LEWGPNWEDDLAGAHVTGP DPNI+KIEEEIKFNFEQ+FMMYLPRLCEHCLN Sbjct: 129 AVTGETMNLEWGPNWEDDLAGAHVTGPKDPNIEKIEEEIKFNFEQAFMMYLPRLCEHCLN 188 Query: 181 PSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFCFP 240 PSCVASCPSGAMYKR+EDGIVLVDQEACRGWRYCMTGCPYKKVYFNW+TNKAEKCTFCFP Sbjct: 189 PSCVASCPSGAMYKRDEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCFP 248 Query: 241 RVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPEVI 300 R+EAGLPTVCSETCTGRIRYLGVLLYDAD+V +AASTPD KDLYQAQ DLF++P+DP VI Sbjct: 249 RIEAGLPTVCSETCTGRIRYLGVLLYDADRVQEAASTPDEKDLYQAQLDLFLDPNDPGVI 308 Query: 301 AQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFEGK 360 QARKDG+SE+W+EAAQNSPVYKLAIE+KLAFPLHPEYRTLPMVWYVPPLSPIMNYFEG+ Sbjct: 309 EQARKDGLSEEWIEAAQNSPVYKLAIEYKLAFPLHPEYRTLPMVWYVPPLSPIMNYFEGR 368 Query: 361 DSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPFDL 420 DS++NPDMIFPA+EEMRTPIQYLAN+LT+GDT TVKEALQRMAMMRSYMRA+S+ FD Sbjct: 369 DSIENPDMIFPAIEEMRTPIQYLANLLTAGDTVTVKEALQRMAMMRSYMRAQSSGAEFDE 428 Query: 421 SRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGYGNRXXXXXX 480 +RL RVG+TAHQ ++MYRLLAIAKY+DRFV+PTSHKE M+ YR+QGS G+G Sbjct: 429 TRLARVGLTAHQMKQMYRLLAIAKYDDRFVVPTSHKESHMDVYRSQGSEGFG-------- 480 Query: 481 XXXXXXXXXXXXXXXXXXTKTAKEIYEENFYGGIWRD 517 KT KE+YEENFYGGIWRD Sbjct: 481 ------ASCGGCGPEGLQGKTGKELYEENFYGGIWRD 511 >B9E9D3.1 B9E9D3_MACCJ Respiratory nitrate reductase beta chain Length = 516 Score = 899 bits (2324), Expect = 0.0, Method: Compositional matrix adjust. Identities = 424/518 (81%), Positives = 461/518 (88%), Gaps = 11/518 (2%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGWQL 60 MVMNLDKCIGCHTCS+TCK+TWTNR GAEYMWFNNVETKPGIGYPKRWEDQE+YKGGWQL Sbjct: 9 MVMNLDKCIGCHTCSITCKSTWTNRPGAEYMWFNNVETKPGIGYPKRWEDQEVYKGGWQL 68 Query: 61 R-KGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 KGKLELKSG+KLSK+ALGKIFYNPDMPEMKDYYEPWTYNY +LT A EQKHSPVA A Sbjct: 69 NSKGKLELKSGSKLSKIALGKIFYNPDMPEMKDYYEPWTYNYANLTNAKEQKHSPVATAE 128 Query: 120 SAVTGEKMDLEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHCL 179 S V+G+KMDL+WGPNWEDDLAGAH+TGP DPNIQKIEEEIKFNFEQSFMMYLPRLCEHCL Sbjct: 129 SLVSGKKMDLQWGPNWEDDLAGAHITGPTDPNIQKIEEEIKFNFEQSFMMYLPRLCEHCL 188 Query: 180 NPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFCF 239 NPSCVASCPSGAMYKR+EDGIVLVDQ+ACRGWRYCMTGCPYKKVYFNW+TNKAEKCTFCF Sbjct: 189 NPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCF 248 Query: 240 PRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPEV 299 PRVEAGLPTVCSETCTGR+RYLGVLLYDAD+VL+AA+ D +DLYQAQ DLF++PHDPEV Sbjct: 249 PRVEAGLPTVCSETCTGRMRYLGVLLYDADRVLEAATVKDPQDLYQAQLDLFLDPHDPEV 308 Query: 300 IAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFEG 359 IAQA +DGI++DW+EAAQNSPVYKLAIE+KLAFPLHPEYRTLPMVWY PPLSPIMNYFEG Sbjct: 309 IAQAERDGIAQDWIEAAQNSPVYKLAIEYKLAFPLHPEYRTLPMVWYCPPLSPIMNYFEG 368 Query: 360 KDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPFD 419 KDS+KNPDMIFPA+EEMR PIQYLANMLT+GD QTVKEALQRMAMMRSYMRA S+ + FD Sbjct: 369 KDSIKNPDMIFPAIEEMRLPIQYLANMLTAGDAQTVKEALQRMAMMRSYMRAVSSGKDFD 428 Query: 420 LSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGYGNRXXXXX 479 SRLDRVGMTAHQ ++MYRLLAIAK+EDRFVIPTSHKE MN Y AQG GY + Sbjct: 429 ASRLDRVGMTAHQVKQMYRLLAIAKHEDRFVIPTSHKEGYMNTYSAQGGEGYTS------ 482 Query: 480 XXXXXXXXXXXXXXXXXXXTKTAKEIYEENFYGGIWRD 517 K+ KE+YE+NFYGGIWRD Sbjct: 483 ----DNFGADCDGCGPVPAGKSGKEVYEDNFYGGIWRD 516 >Q5WK50.1 Q5WK50_BACSK Respiratory nitrate reductase beta chain EC=1.7.99.4 Length = 511 Score = 850 bits (2196), Expect = 0.0, Method: Compositional matrix adjust. Identities = 408/517 (78%), Positives = 450/517 (87%), Gaps = 14/517 (2%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGWQL 60 MVMNLDKCIGCHTCSVTCK+TWTNR GAEY+WFNNVETKPGIGYP RWEDQE YKGGW L Sbjct: 9 MVMNLDKCIGCHTCSVTCKSTWTNRPGAEYIWFNNVETKPGIGYPVRWEDQEQYKGGWTL 68 Query: 61 RKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAHS 120 + GKLELKSG+KLSK+ALG IF+NPDMPEMKDYYEPWTYNYEHLT AGE+KH PVAR S Sbjct: 69 KNGKLELKSGSKLSKIALGSIFHNPDMPEMKDYYEPWTYNYEHLTNAGEKKHQPVARPKS 128 Query: 121 AVTGEKMDLEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHCLN 180 AVTG+ MDLEWGPNWEDDLAG HVTGP DPNI+KIEEEIKFNFEQ+FMMYLPRLCEHCLN Sbjct: 129 AVTGDTMDLEWGPNWEDDLAGGHVTGPRDPNIEKIEEEIKFNFEQTFMMYLPRLCEHCLN 188 Query: 181 PSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFCFP 240 PSCVASCPSGAMYKR+EDGIVLVDQEACRGWRYCMTGCPYKKVYFNW+TNKAEKCTFCFP Sbjct: 189 PSCVASCPSGAMYKRDEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCFP 248 Query: 241 RVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPEVI 300 R+EAGLPTVCSETCTGRIRYLGVLLYDAD+V +AAS + +DLYQ+QCDLF++P+DP +I Sbjct: 249 RIEAGLPTVCSETCTGRIRYLGVLLYDADRVQEAASVANEQDLYQSQCDLFLDPNDPAII 308 Query: 301 AQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFEGK 360 AQAR DG+ E+W+EAAQ SPVYKLAIE+KLAFPLHPEYRTLPMVWYVPPLSPIMN FEGK Sbjct: 309 AQARADGLPEEWIEAAQQSPVYKLAIEYKLAFPLHPEYRTLPMVWYVPPLSPIMNLFEGK 368 Query: 361 DSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPFDL 420 DS+ NPD+IFPA+EEMRTPIQYLAN+LT+GDT+TVK ALQ+MAMMRS MRA S+ FD Sbjct: 369 DSIGNPDLIFPAIEEMRTPIQYLANLLTAGDTETVKGALQKMAMMRSMMRAVSSGADFDE 428 Query: 421 SRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGYGNRXXXXXX 480 RL RVG+T+ Q +EMYRLLAIAKYEDRFVIPTSHKE M+ YR+QG G+G Sbjct: 429 RRLTRVGLTSTQVKEMYRLLAIAKYEDRFVIPTSHKEAYMDVYRSQGDEGFG-------- 480 Query: 481 XXXXXXXXXXXXXXXXXXTKTAKEIYEENFYGGIWRD 517 K+ KE+YEENFYGGIWRD Sbjct: 481 ------LGCTGCGPEGFGGKSGKEMYEENFYGGIWRD 511 >C2G8E2.1 C2G8E2_STAAU Nitrate reductase beta chain EC=1.7.99.4 Length = 519 Score = 836 bits (2160), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/518 (74%), Positives = 443/518 (85%), Gaps = 8/518 (1%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGWQL 60 MV+NLDKCIGCHTCSVTCK TWTNR GAEY+WFNNVETKPG+GYPKRWEDQE YKGGW L Sbjct: 9 MVLNLDKCIGCHTCSVTCKNTWTNRPGAEYLWFNNVETKPGVGYPKRWEDQEHYKGGWVL 68 Query: 61 -RKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 RKGKLELKSG+++SK+ALGKIFYNPDMP +KDYYEPW YNYEHLT A HSPVARA+ Sbjct: 69 NRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGNHSPVARAY 128 Query: 120 SAVTGEKMDLEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHCL 179 S +TG+ +++EWGPNWEDDLAG HVTGP DPNIQKIEE+IKF F+++FMMYLPRLCEHCL Sbjct: 129 SEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYLPRLCEHCL 188 Query: 180 NPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFCF 239 NPSCVASCPSGAMYKR+EDGIVLVDQ+ACRGWRYCMTGCPYKKVYFNW+TNKAEKCTFCF Sbjct: 189 NPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCF 248 Query: 240 PRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPEV 299 PR+EAG+PTVCSETCTGR+RYLGVLLYDAD+V +AAS D KDLY+ Q D+F+NP D EV Sbjct: 249 PRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIFLNPFDEEV 308 Query: 300 IAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFEG 359 IAQA KDGIS DW+EAAQNSP+YKLAIE+KLAFPLHPE+RT+PMVWY PPLSPIM+YFEG Sbjct: 309 IAQAEKDGISYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEG 368 Query: 360 KDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPFD 419 K++ +NPD IFPA+EEMR PI+YLAN+ T+GDT+ VKEALQRMAMMRSYMR++ QPFD Sbjct: 369 KNTNQNPDAIFPAIEEMRLPIEYLANIFTAGDTKPVKEALQRMAMMRSYMRSQVTQQPFD 428 Query: 420 LSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGYGNRXXXXX 479 SRL+R+G+T QT++MYRLL +AKYEDRFVIPTSHKE ++ Y AQGS GY Sbjct: 429 TSRLERLGLTERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGYN------- 481 Query: 480 XXXXXXXXXXXXXXXXXXXTKTAKEIYEENFYGGIWRD 517 KT +EIY ENFYGGI+RD Sbjct: 482 YGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD 519 >Q6GE37.1 Q6GE37_STAAR Nitrate reductase beta chain EC=1.7.99.4 Length = 519 Score = 836 bits (2159), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/518 (74%), Positives = 443/518 (85%), Gaps = 8/518 (1%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGWQL 60 MV+NLDKCIGCHTCSVTCK TWTNR GAEYMWFNNVETKPG+GYPKRWEDQE YKGGW L Sbjct: 9 MVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVL 68 Query: 61 -RKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 RKGKLELKSG+++SK+ALGKIFYNPDMP +KDYYEPW YNYEHLT A HSPVARA+ Sbjct: 69 NRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGNHSPVARAY 128 Query: 120 SAVTGEKMDLEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHCL 179 S +TG+ +++EWGPNWEDDLAG HVTGP DPNI+KIEE+IKF F+++FMMYLPRLCEHCL Sbjct: 129 SEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIKKIEEDIKFQFDETFMMYLPRLCEHCL 188 Query: 180 NPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFCF 239 NPSCVASCPSGAMYKR+EDGIVLVDQ+ACRGWRYCMTGCPYKKVYFNW+TNKAEKCTFCF Sbjct: 189 NPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCF 248 Query: 240 PRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPEV 299 PR+EAG+PTVCSETCTGR+RYLGVLLYDAD+V +AAS D KDLY+ Q D+F+NP D EV Sbjct: 249 PRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIFLNPFDEEV 308 Query: 300 IAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFEG 359 IAQA KDGIS DW+EAAQNSP+YKLAIE+KLAFPLHPE+RT+PMVWY PPLSPIM+YFEG Sbjct: 309 IAQAEKDGISYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEG 368 Query: 360 KDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPFD 419 K++ +NPD IFPA+EEMR PI+YLAN+ T+GDT+ VKEALQRMAMMRSYMR++ QPFD Sbjct: 369 KNTNQNPDAIFPAIEEMRLPIEYLANIFTAGDTKPVKEALQRMAMMRSYMRSQVTQQPFD 428 Query: 420 LSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGYGNRXXXXX 479 SRL+R+G+T QT++MYRLL +AKYEDRFVIPTSHKE ++ Y AQGS GY Sbjct: 429 TSRLERLGLTERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGYN------- 481 Query: 480 XXXXXXXXXXXXXXXXXXXTKTAKEIYEENFYGGIWRD 517 KT +EIY ENFYGGI+RD Sbjct: 482 YGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD 519 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,189,365,460 Number of extensions: 134432015 Number of successful extensions: 302304 Number of sequences better than 1.0e-05: 2923 Number of HSP's gapped: 299220 Number of HSP's successfully gapped: 2925 Length of query: 517 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 377 Effective length of database: 1,741,754,103 Effective search space: 656641296831 Effective search space used: 656641296831 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)