BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0011.p (228 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ64.1 B3KJ64_9BACI Respiratory nitrate reductase, gamma subun... 387 e-106 Q5WK48.1 Q5WK48_BACSK Respiratory nitrate reductase gamma chain ... 366 e-100 Q4L8Q9.1 Q4L8Q9_STAHJ Nitrate reductase gamma chain 345 1e-93 Q8CN74.1 Q8CN74_STAES Nitrate reductase gamma chain 342 2e-92 Q5HLK3.1 Q5HLK3_STAEQ Respiratory nitrate reductase, gamma subun... 342 2e-92 >B3KJ64.1 B3KJ64_9BACI Respiratory nitrate reductase, gamma subunit EC=1.7.99.4 Length = 230 Score = 387 bits (993), Expect = e-106, Method: Compositional matrix adjust. Identities = 175/227 (77%), Positives = 204/227 (89%) Query: 1 MNMFDQFLWVIFPYICLAVFIVGHIYRYRRDQFNWTAKSSEFIEKKQLKYGSILFHLGII 60 MNM DQFLWVIFPY+C+ VFIVGHIYRYR DQFNWTAKSSEFIEKK LK GS++FHLGII Sbjct: 1 MNMIDQFLWVIFPYVCIVVFIVGHIYRYRTDQFNWTAKSSEFIEKKHLKLGSLMFHLGII 60 Query: 61 PVIFGHIGGLAVPKSWMEALGVNDHLYHIGAVYIGGIFGMATLAGMLILTSRRFTIKNVR 120 PV GH+ GLA+P+SWME+LGV+DH+YHIGAVYIG FG TL GM++LT+RRF I+NVR Sbjct: 61 PVTLGHVAGLAIPQSWMESLGVSDHMYHIGAVYIGSAFGFLTLGGMILLTARRFAIRNVR 120 Query: 121 RLSSASDLIVNSLLLFIVFMGMYSTLVTNAVQPEFDYRETISIWFRGLFMFSPDPSLMKE 180 +LS+ASD++VN LLLFIVFMGMY TLVTNAVQPEF+YRET+S+WFRGLFMF P+ SLM++ Sbjct: 121 KLSTASDMVVNVLLLFIVFMGMYGTLVTNAVQPEFNYRETVSVWFRGLFMFRPEASLMED 180 Query: 181 VPVSFKIHVLSGFAIFAFWPFTRLVHVWSVPLNYVGRSYILYRRHKS 227 VP+SFKIH+L+GF IF WPFTRLVHVWSVPLNYVGR YILYR+H+ Sbjct: 181 VPLSFKIHILAGFGIFGLWPFTRLVHVWSVPLNYVGRRYILYRKHRK 227 >Q5WK48.1 Q5WK48_BACSK Respiratory nitrate reductase gamma chain EC=1.7.99.4 Length = 228 Score = 366 bits (939), Expect = e-100, Method: Compositional matrix adjust. Identities = 166/227 (73%), Positives = 201/227 (88%) Query: 1 MNMFDQFLWVIFPYICLAVFIVGHIYRYRRDQFNWTAKSSEFIEKKQLKYGSILFHLGII 60 M++ D FLWVI+PYIC +VF+VGHIYRYR DQFNWTAKSSEF+EKK+L+ GS++FHLGII Sbjct: 1 MSIMDPFLWVIYPYICFSVFVVGHIYRYRTDQFNWTAKSSEFVEKKRLRAGSLMFHLGII 60 Query: 61 PVIFGHIGGLAVPKSWMEALGVNDHLYHIGAVYIGGIFGMATLAGMLILTSRRFTIKNVR 120 PVI GH+ GL +P+SWMEA+GVN+HLYHIGAVYIG FG TLAGM++LT RRFT+KNVR Sbjct: 61 PVIMGHVAGLGIPQSWMEAVGVNEHLYHIGAVYIGSAFGFLTLAGMVLLTFRRFTMKNVR 120 Query: 121 RLSSASDLIVNSLLLFIVFMGMYSTLVTNAVQPEFDYRETISIWFRGLFMFSPDPSLMKE 180 +LSSASD+IVN LLLFI+ +GMYSTLVTNA+QP+F+YRET+S+WFR LF+F PD LM + Sbjct: 121 QLSSASDMIVNVLLLFIIAVGMYSTLVTNALQPDFNYRETVSVWFRQLFLFRPDAGLMID 180 Query: 181 VPVSFKIHVLSGFAIFAFWPFTRLVHVWSVPLNYVGRSYILYRRHKS 227 VP+SFKIH+ +G IFA WPFTRLVHVWSVPLNY+GR YILYR+H+ Sbjct: 181 VPLSFKIHITAGLLIFALWPFTRLVHVWSVPLNYIGRRYILYRKHRK 227 >Q4L8Q9.1 Q4L8Q9_STAHJ Nitrate reductase gamma chain Length = 227 Score = 345 bits (886), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 159/226 (70%), Positives = 192/226 (84%) Query: 3 MFDQFLWVIFPYICLAVFIVGHIYRYRRDQFNWTAKSSEFIEKKQLKYGSILFHLGIIPV 62 M +QFLW+IFPY+C+A+FIVGHI RY+ DQF+WTAKSSEF+EKKQLK+GS+LFHLGIIPV Sbjct: 1 MLNQFLWIIFPYLCVAIFIVGHIARYKYDQFSWTAKSSEFLEKKQLKWGSLLFHLGIIPV 60 Query: 63 IFGHIGGLAVPKSWMEALGVNDHLYHIGAVYIGGIFGMATLAGMLILTSRRFTIKNVRRL 122 FGH+ GLA+P W++ LGV++ +YH GAVYIG IFG+ T GML+LT+RR TIKNVR+L Sbjct: 61 FFGHVVGLAIPAKWIDGLGVSEEMYHFGAVYIGSIFGIMTFIGMLLLTARRITIKNVRKL 120 Query: 123 SSASDLIVNSLLLFIVFMGMYSTLVTNAVQPEFDYRETISIWFRGLFMFSPDPSLMKEVP 182 SSASD+ VN LL+ I+FMG YSTLVTNA+ +FDYR+TISIWFR LF FSPD SLM VP Sbjct: 121 SSASDIFVNFLLILIIFMGCYSTLVTNAMVSDFDYRQTISIWFRHLFTFSPDASLMTSVP 180 Query: 183 VSFKIHVLSGFAIFAFWPFTRLVHVWSVPLNYVGRSYILYRRHKSN 228 +SFKIH+L GF I A WPFTRLVHVWSVPL+Y R YI+YR+HK+ Sbjct: 181 LSFKIHILLGFTILACWPFTRLVHVWSVPLSYTNRRYIVYRKHKTR 226 >Q8CN74.1 Q8CN74_STAES Nitrate reductase gamma chain Length = 225 Score = 342 bits (876), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 156/224 (69%), Positives = 194/224 (86%) Query: 3 MFDQFLWVIFPYICLAVFIVGHIYRYRRDQFNWTAKSSEFIEKKQLKYGSILFHLGIIPV 62 M +QF+WVI+PY+CLA+F++GHI RY+ DQF+WTAKSSE IEKK+LK+GS+LFHLGIIPV Sbjct: 1 MLNQFVWVIYPYLCLAIFVIGHIARYKYDQFSWTAKSSEMIEKKRLKWGSLLFHLGIIPV 60 Query: 63 IFGHIGGLAVPKSWMEALGVNDHLYHIGAVYIGGIFGMATLAGMLILTSRRFTIKNVRRL 122 FGH+ GL +P +W+EA+GVN+H+YHIGAVYIG +FG+ TL GML+LT RR +IKNVRRL Sbjct: 61 FFGHVVGLLIPANWLEAIGVNNHIYHIGAVYIGSVFGIITLIGMLLLTLRRLSIKNVRRL 120 Query: 123 SSASDLIVNSLLLFIVFMGMYSTLVTNAVQPEFDYRETISIWFRGLFMFSPDPSLMKEVP 182 SS SD+ VN +LL I+ MG YSTLVTNA+QPEFDYR+TI+IWFR LFMFSP+ LM VP Sbjct: 121 SSFSDIFVNIVLLIILIMGCYSTLVTNAIQPEFDYRQTIAIWFRHLFMFSPNADLMLNVP 180 Query: 183 VSFKIHVLSGFAIFAFWPFTRLVHVWSVPLNYVGRSYILYRRHK 226 SFK+H+L GF +FA WPFTRLVHVWSVPL+Y+ R YI+YR++K Sbjct: 181 WSFKLHILLGFTVFACWPFTRLVHVWSVPLSYMNRRYIVYRKNK 224 >Q5HLK3.1 Q5HLK3_STAEQ Respiratory nitrate reductase, gamma subunit EC=1.7.99.4 Length = 225 Score = 342 bits (876), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 156/224 (69%), Positives = 194/224 (86%) Query: 3 MFDQFLWVIFPYICLAVFIVGHIYRYRRDQFNWTAKSSEFIEKKQLKYGSILFHLGIIPV 62 M +QF+WVI+PY+CLA+F++GHI RY+ DQF+WTAKSSE IEKK+LK+GS+LFHLGIIPV Sbjct: 1 MLNQFVWVIYPYLCLAIFVIGHIARYKYDQFSWTAKSSEMIEKKRLKWGSLLFHLGIIPV 60 Query: 63 IFGHIGGLAVPKSWMEALGVNDHLYHIGAVYIGGIFGMATLAGMLILTSRRFTIKNVRRL 122 FGH+ GL +P +W+EA+GVN+H+YHIGAVYIG +FG+ TL GML+LT RR +IKNVRRL Sbjct: 61 FFGHVVGLLIPANWLEAIGVNNHIYHIGAVYIGSVFGIITLIGMLLLTLRRLSIKNVRRL 120 Query: 123 SSASDLIVNSLLLFIVFMGMYSTLVTNAVQPEFDYRETISIWFRGLFMFSPDPSLMKEVP 182 SS SD+ VN +LL I+ MG YSTLVTNA+QPEFDYR+TI+IWFR LFMFSP+ LM VP Sbjct: 121 SSFSDIFVNIVLLIILIMGCYSTLVTNAIQPEFDYRQTIAIWFRHLFMFSPNADLMLNVP 180 Query: 183 VSFKIHVLSGFAIFAFWPFTRLVHVWSVPLNYVGRSYILYRRHK 226 SFK+H+L GF +FA WPFTRLVHVWSVPL+Y+ R YI+YR++K Sbjct: 181 WSFKLHILLGFTVFACWPFTRLVHVWSVPLSYMNRRYIVYRKNK 224 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.332 0.145 0.465 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,391,781,792 Number of extensions: 56129274 Number of successful extensions: 152923 Number of sequences better than 1.0e-05: 534 Number of HSP's gapped: 152123 Number of HSP's successfully gapped: 534 Length of query: 228 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 97 Effective length of database: 1,826,543,238 Effective search space: 177174694086 Effective search space used: 177174694086 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 129 (54.3 bits)