BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0010.p (152 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ65.1 B3KJ65_9BACI Control of nitrate reduction 153 4e-36 Q5WK47.1 Q5WK47_BACSK Control of nitrate reduction 147 3e-34 B9E9D6.1 B9E9D6_MACCJ Control of nitrate reduction protein NreA 114 3e-24 C2LWN7.1 C2LWN7_STAHO Putative uncharacterized protein 111 3e-23 B9DL91.1 B9DL91_STACT NreA protein 107 5e-22 >B3KJ65.1 B3KJ65_9BACI Control of nitrate reduction Length = 154 Score = 153 bits (387), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 75/151 (49%), Positives = 101/151 (66%), Gaps = 2/151 (1%) Query: 1 MKNQ--VDYQRELDQIRQALGYDFMSLALADPAEYDYVIRWKYASGNSNSRYKRIVLQSG 58 MKNQ DYQRE+D++R +DF+S+AL PAE +V+ W+YASGN N RYKRIVL SG Sbjct: 1 MKNQPIYDYQREMDKLRTRFHFDFVSMALVQPAEDRFVLTWQYASGNINERYKRIVLHSG 60 Query: 59 RGIAGIVFKTGKPFLLYSVQEQVKQDMLFSYPIINSEKLKSIGAVPLWNDARVAGVLLGG 118 +GIAG+VFKTGKP L+ +V ++ L++YPII +E+LKS+GA+PLW RV GVLL G Sbjct: 61 KGIAGMVFKTGKPMLIPNVNADIESRDLYNYPIIVAEQLKSLGAIPLWEGTRVMGVLLVG 120 Query: 119 FRGDQQVNDTMLRELQNTARLGIGDLNGKEL 149 +R + ++ Q G KE+ Sbjct: 121 YRKENMLDHKAFASFQEGIGTEFGAFYAKEV 151 >Q5WK47.1 Q5WK47_BACSK Control of nitrate reduction Length = 156 Score = 147 bits (371), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 73/145 (50%), Positives = 96/145 (66%) Query: 5 VDYQRELDQIRQALGYDFMSLALADPAEYDYVIRWKYASGNSNSRYKRIVLQSGRGIAGI 64 ++YQ+E+D IR+ +DF+SLAL PAE +V+ W+Y SGN N RYKRIVL SG+G+AGI Sbjct: 9 INYQQEIDDIRKRWKFDFVSLALVQPAEDRFVLTWQYVSGNMNDRYKRIVLHSGKGVAGI 68 Query: 65 VFKTGKPFLLYSVQEQVKQDMLFSYPIINSEKLKSIGAVPLWNDARVAGVLLGGFRGDQQ 124 VFK GK LL +V + LF+YPII SE+LKS+GA+PLW ARV GVLL GFR + Sbjct: 69 VFKIGKAILLQNVNSDIGTRDLFNYPIIVSEQLKSLGALPLWEGARVVGVLLVGFRKENV 128 Query: 125 VNDTMLRELQNTARLGIGDLNGKEL 149 +N Q + G +E+ Sbjct: 129 LNQPSFASFQESLGSTFGAFQIREV 153 >B9E9D6.1 B9E9D6_MACCJ Control of nitrate reduction protein NreA Length = 152 Score = 114 bits (285), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 59/147 (40%), Positives = 85/147 (57%) Query: 6 DYQRELDQIRQALGYDFMSLALADPAEYDYVIRWKYASGNSNSRYKRIVLQSGRGIAGIV 65 DYQ +DQ+R++L DF+ LA+ I W+Y SG +N RYK+I L+ G+G+AGIV Sbjct: 3 DYQAMIDQLRESLNVDFIGLAMPSDLILQTDIHWRYVSGETNERYKKIELKKGKGVAGIV 62 Query: 66 FKTGKPFLLYSVQEQVKQDMLFSYPIINSEKLKSIGAVPLWNDARVAGVLLGGFRGDQQV 125 K G+ ++ ++ Q LF +PII EKL S AVPLW +VA VLL G R + Sbjct: 63 IKIGRDWIELDIESSSLQTHLFDFPIIRFEKLTSFIAVPLWKYNKVAAVLLIGNRSKRPF 122 Query: 126 NDTMLRELQNTARLGIGDLNGKELLLS 152 TM +L+ G+G K+++ S Sbjct: 123 TLTMHEQLKKNLEQGLGAFYCKDVINS 149 >C2LWN7.1 C2LWN7_STAHO Putative uncharacterized protein Length = 150 Score = 111 bits (277), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 54/121 (44%), Positives = 79/121 (65%) Query: 5 VDYQRELDQIRQALGYDFMSLALADPAEYDYVIRWKYASGNSNSRYKRIVLQSGRGIAGI 64 V +Q+ELD +R+ DF +AL + E I+W+Y SGN N+RYK IVL+ GRG+AG Sbjct: 10 VHFQQELDTLRKKENCDFAGVALYENHEPSGPIKWQYVSGNLNNRYKLIVLRKGRGLAGT 69 Query: 65 VFKTGKPFLLYSVQEQVKQDMLFSYPIINSEKLKSIGAVPLWNDARVAGVLLGGFRGDQQ 124 V KTGK ++ +V + + + YPI+ SE L ++ A+PLW++ VAGVLL G RG + Sbjct: 70 VMKTGKRMVIENVSKTITPEEKIKYPILMSENLNALVAIPLWHEQHVAGVLLLGQRGSKT 129 Query: 125 V 125 + Sbjct: 130 L 130 >B9DL91.1 B9DL91_STACT NreA protein Length = 155 Score = 107 bits (266), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 52/115 (45%), Positives = 76/115 (66%) Query: 6 DYQRELDQIRQALGYDFMSLALADPAEYDYVIRWKYASGNSNSRYKRIVLQSGRGIAGIV 65 DYQ LD+IR+ +DF ++AL + + VI+WKYASGN N RY+ IVL+ G+G+AG+V Sbjct: 12 DYQDALDEIRETEKFDFAAIALPEDGLHSAVIKWKYASGNINYRYRMIVLRPGKGLAGLV 71 Query: 66 FKTGKPFLLYSVQEQVKQDMLFSYPIINSEKLKSIGAVPLWNDARVAGVLLGGFR 120 +TG ++ V ++ Q+ YPI+ SE L ++ A+PLW + RV G LL G R Sbjct: 72 IRTGSRKIVEDVDAELSQNDKLGYPIVLSEALTAMVAIPLWKNNRVYGALLLGQR 126 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 905,150,935 Number of extensions: 34941064 Number of successful extensions: 74492 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 74495 Number of HSP's successfully gapped: 34 Length of query: 152 Length of database: 3,060,696,203 Length adjustment: 114 Effective length of query: 38 Effective length of database: 1,986,700,493 Effective search space: 75494618734 Effective search space used: 75494618734 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)